Objectives: To explore the effect of acupuncture on cerebral infarction in rats and to try providing some experimental parameters for clinical practice. Methods: 27 healthy Wistar rats were randomly divided into pseud...Objectives: To explore the effect of acupuncture on cerebral infarction in rats and to try providing some experimental parameters for clinical practice. Methods: 27 healthy Wistar rats were randomly divided into pseudo operation (n=10), model (n=8) and acupuncture (n=9) groups. Neuro functional defect scoring, apoptosis of single brain slice and the number of bcl 2 immuno reaction (IR) positive neurons in CA1 area of the hippocampus were used as the indexes to investigate the possible mechanisms of acupuncture of "Nie San Zhen" (needling three acupoints in the temporal region) and "Si Shen Zhen" (needling four acupoints in the occiput region) in treating rats with cerebral infarction. Results: There existed significant differences between acupuncture group and model group in improving neurologic functional activities, inhibiting apoptosis of the brain cells and increasing bcl 2 IR positive neurons in the hippocampal CA1 area ( P <0.01). Conclusion: Acupuncture therapy can improve cerebral infarction in the rat by suppressing apoptosis and up regulation of the bcl 2 IR positive neuron expression.展开更多
Diabetes mellitus （DM） is a metabolic syndrome, characterized by long-standing hyperglycemia. The incidence of type 2 diabetes is higher than 90%. Treatment of diabetes by acupuncture existed in ancient times, and a...Diabetes mellitus （DM） is a metabolic syndrome, characterized by long-standing hyperglycemia. The incidence of type 2 diabetes is higher than 90%. Treatment of diabetes by acupuncture existed in ancient times, and as early as in Zhen Jiu Jia Yi Jing （A-B Canon of Acupuncture and Moxibustion）, it documented the points selection therapy in treating diabetes. In recent years, according to traditional Chinese medicine （TCM） theory, I treated 35 patients of type 2 diabetes by acupuncture therapy, and now it is reported as follows.展开更多
Objective:To observe the effects of electroacupuncture(EA)on the protein and gene expressions of Bax,Caspase-3 and Bcl-2 in cerebral cortex of type 2 diabetic rats with cognitive impairment(CI),and to explore the mech...Objective:To observe the effects of electroacupuncture(EA)on the protein and gene expressions of Bax,Caspase-3 and Bcl-2 in cerebral cortex of type 2 diabetic rats with cognitive impairment(CI),and to explore the mechanism of EA in improving the learning and memory abilities.Methods:A total of 100 Sprague-Dawley(SD)rats were divided into a normal group(n=10)and a model group(n=90)by random number table method.Rats in the model group were intraperitoneally injected with a small dose of streptozotocin(STZ)to establish the type 2 diabetic models,after being fed with high-fat and high-sugar diet for 1 month.Twenty CI rats were selected from the 50 successful model rats by the Morris water maze(MWM)test and randomly divided into a model group and an EA group according to the blood glucose level and MWM data(n=10).Rats in the EA group received acupuncture at Zusanli(ST 36),Neiting(ST 44)and Yishu(Extra),of which Zusanli(ST 36)and Neiting(ST 44)were stimulated by EA apparatus,20 min/time,once a day for 6 d a week and 4 consecutive weeks.The rats in the model and the normal groups were fixed without treatment.After 4-week treatment,the random blood glucose level of the rats was measured;the learning and memory abilities of rats were measured by MWM;terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL)assay was used to detect apoptotic cells;Western blot(WB)and real-time quantitative polymerase chain reaction(RT-qPCR)were used to detect the protein and gene expressions of Bax,Caspase-3 and Bcl-2 in cerebral cortex.Results:After modeling,the random blood glucose level and the escape latency tested by MWM were significantly increased,and the number of crossing the platform tested by the MWM was decreased in the EA and model groups,and were significantly different from those in the normal group(P<0.05 or P<0.01),while the differences between the model group and the EA group were not statistically significant(all P>0.05).After 4-week treatment,the random glucose level and the escape latency tested by MWM were significantly increased(both P<0.05),and the number of crossing the original platform tested by the MWM was significantly reduced(P<0.01),the protein and gene expressions of Bax and Caspase-3 were significantly increased(all P<0.001),the protein and gene expressions of Bcl-2 were significantly reduced(both P<0.001),and the number of neuron apoptosis was significantly increased(P<0.001)in the model group than in the normal group;the random blood glucose level was significantly reduced(P<0.05),the escape latency tested by MWM was significantly shortened(P<0.05),and the number of crossing the original platform tested by MWM was significantly increased(P<0.05),the protein and gene expressions of Bax and Caspase-3 were significantly reduced(all P<0.001),the protein and gene expressions of Bcl-2 were significantly increased(both P<0.001),and the number of neuron apoptosis was significantly reduced(P<0.001)in the EA group than in the model group.Conclusion:EA can improve the learning and memory damages induced by type 2 diabetic model rats with CI;the action mechanism may be achieved via anti-apoptosis.展开更多
Objective： To observe the effect of acupuncture on gastric motility, plasma motilin and serum gastrin in patients with diabetic gastroparesis （DGP） and evaluate its clinical efficacy. Methods： A total of 100 eligi...Objective： To observe the effect of acupuncture on gastric motility, plasma motilin and serum gastrin in patients with diabetic gastroparesis （DGP） and evaluate its clinical efficacy. Methods： A total of 100 eligible cases were randomly allocated into an acupuncture group （n=50） and a control group （n=50）. Patients in the acupuncture group were treated by needling Zhongwan （CV 12）, Zusanli （ST 36） and Neiguan （PC 6）, whereas patients in the control group were treated with oral administration of Domperidone. The clinical efficacies of the two groups were compared; and changes in gastric motility, plasma motilin and serum gastrin in both groups were observed before and after treatment. Results： After treatment, the symptom scores, gastric motility and contents of plasma motilin and serum gastrin were significantly improved in both groups （P〈0.05）. There were between-group statistically significant differences in symptom scores, gastric motility and levels of plasma motilin and serum gastrin after treatment （all P〈0.05）. The total effective rate was 96% in the treatment group, versus 78% in the control group, showing a statistically significant difference （P〈0.05）. Conclusion： Acupuncture is effective for DGP and can reduce the levels of plasma motilin and serum gastrin.展开更多
Objective:To review the systematic reviews of acupuncture for diabetic peripheral neuropathy(DPN)and to provide evidence for clinical decisions.Methods:Published systematic reviews targeting acupuncture treatment of D...Objective:To review the systematic reviews of acupuncture for diabetic peripheral neuropathy(DPN)and to provide evidence for clinical decisions.Methods:Published systematic reviews targeting acupuncture treatment of DPN were searched using computer through both Chinese and English databases till July 1,2019.Two researchers screened the papers based on inclusion and exclusion criteria and conducted report quality evaluation,methodological quality assessment and evidence quality grading using the preferred reporting items for systematic reviews and meta-analyses(PRISMA),assessment of multiple systematic review 2(AMSTAR 2)and grading of recommendations assessment,development and evaluation(GRADE).Results:Ten systematic reviews were included,involving 11 outcome measures.According to PRISMA,6 items were sufficiently reported while 1 item was not;AMSTAR 2 appraised that all the included systematic reviews were of low quality in the methodological evaluation;according to GRADE,of the 30 clinical evidences,only 5 were graded moderate while the remained were graded low or extremely low.Descriptive analysis showed that acupuncture can significantly improve DPN symptoms,accelerate the conduction velocities of sensory and motor nerves,and up-regulate the content of plasma nitric oxide(NO),while the adverse reaction rate was low.Conclusion:Acupuncture can produce satisfactory clinical efficacy in treating DPN,but the existing problems,such as low-quality evidence,unitary outcome measures,poor methodological quality of systematic reviews and nonstandard reporting,need to be treated cautiously;meanwhile,more high-quality clinical trials are required to elevate the level of evidence.展开更多
文摘Objectives: To explore the effect of acupuncture on cerebral infarction in rats and to try providing some experimental parameters for clinical practice. Methods: 27 healthy Wistar rats were randomly divided into pseudo operation (n=10), model (n=8) and acupuncture (n=9) groups. Neuro functional defect scoring, apoptosis of single brain slice and the number of bcl 2 immuno reaction (IR) positive neurons in CA1 area of the hippocampus were used as the indexes to investigate the possible mechanisms of acupuncture of "Nie San Zhen" (needling three acupoints in the temporal region) and "Si Shen Zhen" (needling four acupoints in the occiput region) in treating rats with cerebral infarction. Results: There existed significant differences between acupuncture group and model group in improving neurologic functional activities, inhibiting apoptosis of the brain cells and increasing bcl 2 IR positive neurons in the hippocampal CA1 area ( P <0.01). Conclusion: Acupuncture therapy can improve cerebral infarction in the rat by suppressing apoptosis and up regulation of the bcl 2 IR positive neuron expression.
文摘Diabetes mellitus （DM） is a metabolic syndrome, characterized by long-standing hyperglycemia. The incidence of type 2 diabetes is higher than 90%. Treatment of diabetes by acupuncture existed in ancient times, and as early as in Zhen Jiu Jia Yi Jing （A-B Canon of Acupuncture and Moxibustion）, it documented the points selection therapy in treating diabetes. In recent years, according to traditional Chinese medicine （TCM） theory, I treated 35 patients of type 2 diabetes by acupuncture therapy, and now it is reported as follows.
文摘Objective:To observe the effects of electroacupuncture(EA)on the protein and gene expressions of Bax,Caspase-3 and Bcl-2 in cerebral cortex of type 2 diabetic rats with cognitive impairment(CI),and to explore the mechanism of EA in improving the learning and memory abilities.Methods:A total of 100 Sprague-Dawley(SD)rats were divided into a normal group(n=10)and a model group(n=90)by random number table method.Rats in the model group were intraperitoneally injected with a small dose of streptozotocin(STZ)to establish the type 2 diabetic models,after being fed with high-fat and high-sugar diet for 1 month.Twenty CI rats were selected from the 50 successful model rats by the Morris water maze(MWM)test and randomly divided into a model group and an EA group according to the blood glucose level and MWM data(n=10).Rats in the EA group received acupuncture at Zusanli(ST 36),Neiting(ST 44)and Yishu(Extra),of which Zusanli(ST 36)and Neiting(ST 44)were stimulated by EA apparatus,20 min/time,once a day for 6 d a week and 4 consecutive weeks.The rats in the model and the normal groups were fixed without treatment.After 4-week treatment,the random blood glucose level of the rats was measured;the learning and memory abilities of rats were measured by MWM;terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL)assay was used to detect apoptotic cells;Western blot(WB)and real-time quantitative polymerase chain reaction(RT-qPCR)were used to detect the protein and gene expressions of Bax,Caspase-3 and Bcl-2 in cerebral cortex.Results:After modeling,the random blood glucose level and the escape latency tested by MWM were significantly increased,and the number of crossing the platform tested by the MWM was decreased in the EA and model groups,and were significantly different from those in the normal group(P<0.05 or P<0.01),while the differences between the model group and the EA group were not statistically significant(all P>0.05).After 4-week treatment,the random glucose level and the escape latency tested by MWM were significantly increased(both P<0.05),and the number of crossing the original platform tested by the MWM was significantly reduced(P<0.01),the protein and gene expressions of Bax and Caspase-3 were significantly increased(all P<0.001),the protein and gene expressions of Bcl-2 were significantly reduced(both P<0.001),and the number of neuron apoptosis was significantly increased(P<0.001)in the model group than in the normal group;the random blood glucose level was significantly reduced(P<0.05),the escape latency tested by MWM was significantly shortened(P<0.05),and the number of crossing the original platform tested by MWM was significantly increased(P<0.05),the protein and gene expressions of Bax and Caspase-3 were significantly reduced(all P<0.001),the protein and gene expressions of Bcl-2 were significantly increased(both P<0.001),and the number of neuron apoptosis was significantly reduced(P<0.001)in the EA group than in the model group.Conclusion:EA can improve the learning and memory damages induced by type 2 diabetic model rats with CI;the action mechanism may be achieved via anti-apoptosis.
基金supported by 2011 Science and Technology Planning Project of Wenzhou City,Zhejiang Province(No.Y20110240)~~
文摘Objective： To observe the effect of acupuncture on gastric motility, plasma motilin and serum gastrin in patients with diabetic gastroparesis （DGP） and evaluate its clinical efficacy. Methods： A total of 100 eligible cases were randomly allocated into an acupuncture group （n=50） and a control group （n=50）. Patients in the acupuncture group were treated by needling Zhongwan （CV 12）, Zusanli （ST 36） and Neiguan （PC 6）, whereas patients in the control group were treated with oral administration of Domperidone. The clinical efficacies of the two groups were compared; and changes in gastric motility, plasma motilin and serum gastrin in both groups were observed before and after treatment. Results： After treatment, the symptom scores, gastric motility and contents of plasma motilin and serum gastrin were significantly improved in both groups （P〈0.05）. There were between-group statistically significant differences in symptom scores, gastric motility and levels of plasma motilin and serum gastrin after treatment （all P〈0.05）. The total effective rate was 96% in the treatment group, versus 78% in the control group, showing a statistically significant difference （P〈0.05）. Conclusion： Acupuncture is effective for DGP and can reduce the levels of plasma motilin and serum gastrin.
文摘Objective:To review the systematic reviews of acupuncture for diabetic peripheral neuropathy(DPN)and to provide evidence for clinical decisions.Methods:Published systematic reviews targeting acupuncture treatment of DPN were searched using computer through both Chinese and English databases till July 1,2019.Two researchers screened the papers based on inclusion and exclusion criteria and conducted report quality evaluation,methodological quality assessment and evidence quality grading using the preferred reporting items for systematic reviews and meta-analyses(PRISMA),assessment of multiple systematic review 2(AMSTAR 2)and grading of recommendations assessment,development and evaluation(GRADE).Results:Ten systematic reviews were included,involving 11 outcome measures.According to PRISMA,6 items were sufficiently reported while 1 item was not;AMSTAR 2 appraised that all the included systematic reviews were of low quality in the methodological evaluation;according to GRADE,of the 30 clinical evidences,only 5 were graded moderate while the remained were graded low or extremely low.Descriptive analysis showed that acupuncture can significantly improve DPN symptoms,accelerate the conduction velocities of sensory and motor nerves,and up-regulate the content of plasma nitric oxide(NO),while the adverse reaction rate was low.Conclusion:Acupuncture can produce satisfactory clinical efficacy in treating DPN,but the existing problems,such as low-quality evidence,unitary outcome measures,poor methodological quality of systematic reviews and nonstandard reporting,need to be treated cautiously;meanwhile,more high-quality clinical trials are required to elevate the level of evidence.
