目的建立能同时测定冠心安口服液中蒙花苷、延胡索乙素和2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷含量的HPLC分析方法。方法Capcell Pak UG C_(18)色谱柱分离;蒙花苷、延胡索乙素和2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷检测...目的建立能同时测定冠心安口服液中蒙花苷、延胡索乙素和2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷含量的HPLC分析方法。方法Capcell Pak UG C_(18)色谱柱分离;蒙花苷、延胡索乙素和2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷检测波长分别设定为334、281、320 nm;柱温为室温;进样体积为10μL;以乙腈为流动相A,1 mL·L^(−1)磷酸溶液(三乙胺调节pH至6.0)为流动相B,梯度洗脱。结果蒙花苷、延胡索乙素和2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的质量浓度分别在0.0245~2.4500、0.0457~4.5700、0.0474~4.7400μg·mL^(−1)范围内线性良好;平均回收率分别为100.8%、99.9%、101.4%;精密度和重复性RSD值(n=6)均在5.0%以内;供试品溶液在24 h内稳定。结论此方法具有前处理简单、分析时间短和检测结果准确等优点,适用于冠心安口服液制剂的质量控制。展开更多
目的:分析25-羟基维生素D与DPN之间的关系。方法:选取2023年2月至2023年12月期间,在北华大学附属医院内分泌科住院的2型糖尿病患者99例,其中68例合并了周围神经病变(DPN),31例未合并周围神经病变(NDPN)。对两组之间的一般资料进行差异...目的:分析25-羟基维生素D与DPN之间的关系。方法:选取2023年2月至2023年12月期间,在北华大学附属医院内分泌科住院的2型糖尿病患者99例,其中68例合并了周围神经病变(DPN),31例未合并周围神经病变(NDPN)。对两组之间的一般资料进行差异性分析;logistic回归分析25-羟基维生素D与DPN之间的关系,并绘制受试者工作特征曲线(ROC)。结果:DPN组患者的年龄、糖尿病病程高于NDPN组患者(P P P P Objective: To analyze the relationship between 25-hydroxyvitamin D and DPN. Method: From February 2023 to December 2023, 99 patients with type 2 diabetes mellitus were hospitalized in the Department of Endocrinology, Affiliated Hospital of Beihua University, of which 68 had peripheral neuropathy (DPN) and 31 had no peripheral neuropathy (NDPN). Differences were analysed for general data between the two groups;logistic regression analysis was used to analyze the relationship between 25-hydroxyvitamin D and DPN, and the receiver operating characteristic curve (ROC) was plotted. Results: The age and duration of diabetes mellitus in the DPN group were higher than those in the NDPN group (P P P P < 0.05). Logistic regression multivariate analysis showed that the incidence of DPN increased by 19.1% for every 1 ng/ml decrease in 25-hydroxyvitamin D. The ROC curve area is 0.721. Conclusion: The level of 25-hydroxyvitamin D in patients with type 2 diabetes mellitus is significantly correlated with DPN. Evaluation of 25-hydroxyvitamin D may be a valuable part of the follow-up of patients with type 2 diabetes.展开更多
文摘目的建立能同时测定冠心安口服液中蒙花苷、延胡索乙素和2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷含量的HPLC分析方法。方法Capcell Pak UG C_(18)色谱柱分离;蒙花苷、延胡索乙素和2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷检测波长分别设定为334、281、320 nm;柱温为室温;进样体积为10μL;以乙腈为流动相A,1 mL·L^(−1)磷酸溶液(三乙胺调节pH至6.0)为流动相B,梯度洗脱。结果蒙花苷、延胡索乙素和2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的质量浓度分别在0.0245~2.4500、0.0457~4.5700、0.0474~4.7400μg·mL^(−1)范围内线性良好;平均回收率分别为100.8%、99.9%、101.4%;精密度和重复性RSD值(n=6)均在5.0%以内;供试品溶液在24 h内稳定。结论此方法具有前处理简单、分析时间短和检测结果准确等优点,适用于冠心安口服液制剂的质量控制。
文摘目的:分析25-羟基维生素D与DPN之间的关系。方法:选取2023年2月至2023年12月期间,在北华大学附属医院内分泌科住院的2型糖尿病患者99例,其中68例合并了周围神经病变(DPN),31例未合并周围神经病变(NDPN)。对两组之间的一般资料进行差异性分析;logistic回归分析25-羟基维生素D与DPN之间的关系,并绘制受试者工作特征曲线(ROC)。结果:DPN组患者的年龄、糖尿病病程高于NDPN组患者(P P P P Objective: To analyze the relationship between 25-hydroxyvitamin D and DPN. Method: From February 2023 to December 2023, 99 patients with type 2 diabetes mellitus were hospitalized in the Department of Endocrinology, Affiliated Hospital of Beihua University, of which 68 had peripheral neuropathy (DPN) and 31 had no peripheral neuropathy (NDPN). Differences were analysed for general data between the two groups;logistic regression analysis was used to analyze the relationship between 25-hydroxyvitamin D and DPN, and the receiver operating characteristic curve (ROC) was plotted. Results: The age and duration of diabetes mellitus in the DPN group were higher than those in the NDPN group (P P P P < 0.05). Logistic regression multivariate analysis showed that the incidence of DPN increased by 19.1% for every 1 ng/ml decrease in 25-hydroxyvitamin D. The ROC curve area is 0.721. Conclusion: The level of 25-hydroxyvitamin D in patients with type 2 diabetes mellitus is significantly correlated with DPN. Evaluation of 25-hydroxyvitamin D may be a valuable part of the follow-up of patients with type 2 diabetes.
