目的:优选Alcalase AF 2.4L碱性蛋白酶水解马鹿茸生成多肽的工艺。方法:采用L9(34)正交试验,通过甲醛滴定法测得多肽得率为评价指标,考察酶解温度、加酶量、PH和酶解时间等因素对酶解工艺的影响。结果:酶解过程在温度60℃,PH7.5,加酶量...目的:优选Alcalase AF 2.4L碱性蛋白酶水解马鹿茸生成多肽的工艺。方法:采用L9(34)正交试验,通过甲醛滴定法测得多肽得率为评价指标,考察酶解温度、加酶量、PH和酶解时间等因素对酶解工艺的影响。结果:酶解过程在温度60℃,PH7.5,加酶量4%,酶解1.5 h后多肽获得率最高。结论:分析正交试验结果,优选马鹿茸多肽最佳酶解工艺。展开更多
The formation of DNA-adducts, i.e. the covalent modification of DNA molecules with carcinogens, is the initial event in carcinogenesis. In the organisms, most of the genotoxic chemicals must be first converted into el...The formation of DNA-adducts, i.e. the covalent modification of DNA molecules with carcinogens, is the initial event in carcinogenesis. In the organisms, most of the genotoxic chemicals must be first converted into electrophilic metabolites, and then covalently hind with the nucleophilic sites of DNA molecules to form DNA-adducts.展开更多
文摘目的:优选Alcalase AF 2.4L碱性蛋白酶水解马鹿茸生成多肽的工艺。方法:采用L9(34)正交试验,通过甲醛滴定法测得多肽得率为评价指标,考察酶解温度、加酶量、PH和酶解时间等因素对酶解工艺的影响。结果:酶解过程在温度60℃,PH7.5,加酶量4%,酶解1.5 h后多肽获得率最高。结论:分析正交试验结果,优选马鹿茸多肽最佳酶解工艺。
文摘The formation of DNA-adducts, i.e. the covalent modification of DNA molecules with carcinogens, is the initial event in carcinogenesis. In the organisms, most of the genotoxic chemicals must be first converted into electrophilic metabolites, and then covalently hind with the nucleophilic sites of DNA molecules to form DNA-adducts.
