An efficient metal-free strategy for the synthesis of pharmaceutically relevant benzo[α]carbazoles from the derivatives of readily available 2-phenylindole and bio-renewable acetol in an aqueous biphasic system was d...An efficient metal-free strategy for the synthesis of pharmaceutically relevant benzo[α]carbazoles from the derivatives of readily available 2-phenylindole and bio-renewable acetol in an aqueous biphasic system was developed. This protocol employed a sulfone-containing Bronsted acidic ionic liquid as the catalyst, which could be used for five times without a noticeable decrease in its activity and selectivity. Various substituted 2-phenylindoles and α-hydroxyketones participated in the reaction smoothly, with water as the sole byproduct. Mechanistically, the reaction involved the conventional carbon-nucleophile-induced Heyns-type rearrangement and downstream intramolecular olefination.展开更多
Recently, it has been reported that the luteolytic ac-tion was the main mechanism of the termination of earlypregnancy by contraceptivest. In addition, some estro-gen receptor ligands such as droloxifene have beenshow...Recently, it has been reported that the luteolytic ac-tion was the main mechanism of the termination of earlypregnancy by contraceptivest. In addition, some estro-gen receptor ligands such as droloxifene have beenshown to inhibit the growth of cultured rat luteal cells.It has been known that 2-phenylindole derivatives couldcompete with estradiol for the estrogen receptor. Theseresults prompted us to design and synthesize the follow-ing six new 3-oxamoyl-2-phenylindole compounds 1a-fand test their effects on the growth of luteal cells, aimingat seeking new lead compounds possessing antifertility activities.展开更多
The fluorescent dye 4′,6-diamidino-2-phenylindole (DAPI) has been widely used to stain microorganisms in various environment media. We applied DAPI fluorescence enumeration to airborne microorganisms and found that n...The fluorescent dye 4′,6-diamidino-2-phenylindole (DAPI) has been widely used to stain microorganisms in various environment media. We applied DAPI fluorescence enumeration to airborne microorganisms and found that non-biological particles, including organic compounds, minerals, and soot, were also visible upon exposure to UV excitation under fluorescence microscope. Using laboratory-prepared biological particles as the control, we investigated the feasibility of identifying both biological and non-biological particles in the same sample with DAPI staining. We prepared biological (bacterial, fungi, and plant detritus) and non-biological (biochar, soot, mineral, metal, fly ash, salt) particles in the laboratory and enumerated the particles and their mixture with DAPI. We found that mineral particles were transparent, and biochar, soot, metals and fly ash particles were black under a filter set at excitation 350/50 nm and emission 460/50 nm bandpass (DAPI-BP), while biological particles were blue, as expected. Particles of the water-soluble salts NaCl and (NH_(4))_(2)SO_(4) were yellow under a filter set at excitation 340–380 nm and emission 425 nm long pass (DAPI-LP). Case studies with samples of dustfall, atmospheric aerosols and surface soils could allow for the quantification of the relative number of different types of particles and particles with organic matter or salt coating as well. Fluorescence enumeration with DAPI stain is thus able to identify the co-existence of biological and non-biological particles in the air, at least to the extent of those examined in this study.展开更多
基金supported by the National Natural Science Foundation of China(21761132014,21872060)the Fundamental Research Funds for the Central Universities of China(2016YXZD033)+1 种基金the Fundamental Research Funds for the Central Universities(2019kfyXJJS072)Opening fund of Hubei Key Laboratory of Material Chemistry and Service Failure(2017MCF01K)~~
文摘An efficient metal-free strategy for the synthesis of pharmaceutically relevant benzo[α]carbazoles from the derivatives of readily available 2-phenylindole and bio-renewable acetol in an aqueous biphasic system was developed. This protocol employed a sulfone-containing Bronsted acidic ionic liquid as the catalyst, which could be used for five times without a noticeable decrease in its activity and selectivity. Various substituted 2-phenylindoles and α-hydroxyketones participated in the reaction smoothly, with water as the sole byproduct. Mechanistically, the reaction involved the conventional carbon-nucleophile-induced Heyns-type rearrangement and downstream intramolecular olefination.
文摘Recently, it has been reported that the luteolytic ac-tion was the main mechanism of the termination of earlypregnancy by contraceptivest. In addition, some estro-gen receptor ligands such as droloxifene have beenshown to inhibit the growth of cultured rat luteal cells.It has been known that 2-phenylindole derivatives couldcompete with estradiol for the estrogen receptor. Theseresults prompted us to design and synthesize the follow-ing six new 3-oxamoyl-2-phenylindole compounds 1a-fand test their effects on the growth of luteal cells, aimingat seeking new lead compounds possessing antifertility activities.
基金supports from the Open Fund of Hubei Key Laboratory of Mine Environmental Pollution Control and Remediation(grant No.2014104)the Research Project of Hubei Provincial Department of Education(grant No.D20184502)the National Natural Science Foundation of China(grant No.42107088).
文摘The fluorescent dye 4′,6-diamidino-2-phenylindole (DAPI) has been widely used to stain microorganisms in various environment media. We applied DAPI fluorescence enumeration to airborne microorganisms and found that non-biological particles, including organic compounds, minerals, and soot, were also visible upon exposure to UV excitation under fluorescence microscope. Using laboratory-prepared biological particles as the control, we investigated the feasibility of identifying both biological and non-biological particles in the same sample with DAPI staining. We prepared biological (bacterial, fungi, and plant detritus) and non-biological (biochar, soot, mineral, metal, fly ash, salt) particles in the laboratory and enumerated the particles and their mixture with DAPI. We found that mineral particles were transparent, and biochar, soot, metals and fly ash particles were black under a filter set at excitation 350/50 nm and emission 460/50 nm bandpass (DAPI-BP), while biological particles were blue, as expected. Particles of the water-soluble salts NaCl and (NH_(4))_(2)SO_(4) were yellow under a filter set at excitation 340–380 nm and emission 425 nm long pass (DAPI-LP). Case studies with samples of dustfall, atmospheric aerosols and surface soils could allow for the quantification of the relative number of different types of particles and particles with organic matter or salt coating as well. Fluorescence enumeration with DAPI stain is thus able to identify the co-existence of biological and non-biological particles in the air, at least to the extent of those examined in this study.
