目的探讨外周血富含半胱氨酸蛋白61(cysteine-rich protein 61,CCN1)与2型糖尿病患者早期肾损伤的相关性研究。方法回顾性研究河北省石家庄市第二医院2型糖尿病患者321例,以尿液微量白蛋白排泄率(urinary albumin excretion rate,UAER)...目的探讨外周血富含半胱氨酸蛋白61(cysteine-rich protein 61,CCN1)与2型糖尿病患者早期肾损伤的相关性研究。方法回顾性研究河北省石家庄市第二医院2型糖尿病患者321例,以尿液微量白蛋白排泄率(urinary albumin excretion rate,UAER)为金标准,将患者分成将2型糖尿病患者分成早期肾损伤组(n=78,20μg/min≤UAER≤200μg/min)、糖尿病肾病组(n=28,UAER>200μg/min)和正常组(n=215,UAER<20μg/min)。比较3组外周血CCN1表达,Pearson法测定UAER与外周血CCN1的相关性,使用ROC曲线评估外周血CCN1对2型糖尿病早期肾损伤和糖尿病肾病的预测价值。单因素分析基线资料,多因素Logistic回归分析确定早期肾损伤或糖尿病肾病的影响因素。结果糖尿病肾病组CCN1和UAER水平高于早期肾损伤组和正常组;早期肾损伤组的CCN1和UAER水平也高于正常组(P<0.05);外周血CCN1与UAER呈正相关(r=0.916,P<0.001)。以外周血CCN1为检验变量,以是否发生早期肾损伤为状态标量,以敏感度为Y轴,以“1-特异度”为X轴绘制ROC曲线。ROC结果显示,CCN1诊断的临界值为1.59μg/L,其对应的敏感度为85.85%,特异度为73.02%,AUC为0.871(95%CI:0.831~0.903)。以外周血CCN1为检验变量,以是否发生糖尿病肾病为状态标量,以敏感度为Y轴,以“1-特异度”为X轴绘制ROC曲线。ROC结果显示,CCN1诊断的临界值为3.41μg/L,其对应的敏感度为71.43%,特异度为73.72%,AUC为0.761(95%CI:0.707~0.813)。糖尿病肾病组、早期肾损伤组和正常组的病程、高血压、高血脂、收缩压、FPG、糖化血红蛋白(glycated hemoglobin,HbA1c)、尿酸(uric acid,UA)、总胆固醇(total cholesterol,TC)、三酰甘油(triglycerides,TG)和低密度脂蛋白(low-density lipoprotein,LDL)等方面比较差异有统计学意义(P<0.05)。Logistic回归分析结果显示,病程(OR=1.740,95%CI:1.180~2.565)、收缩压(OR=1.943,95%CI:1.267~2.978)、高血脂(OR=1.654,95%CI:1.310~2.088)、FPG(OR=2.776,95%CI:1.260~6.116)、UA(OR=2.228,95%CI:1.174~4.229)和CCN1(OR=3.744,95%CI:1.709~8.200)是2型糖尿病早期肾损伤发生的影响因素(P<0.05)。Logistic回归分析结果显示,病程(OR=1.752,95%CI:1.177~2.609)、收缩压(OR=1.818,95%CI:1.034~3.198)、高血脂(OR=1.680,95%CI:1.320~2.138)、FPG(OR=2.389,95%CI:1.519~3.758)、UA(OR=1.260,95%CI:1.038~1.530)和CCN1(OR=3.815,95%CI:1.636~8.897)是糖尿病肾病发生的影响因素(P<0.05)。结论外周血CCN1与尿液微量白蛋白排泄率呈正相关,是早期肾损伤或糖尿病肾病的危险因素,并可应用于预测早期肾损伤或糖尿病肾病的发生,值得临床进一步研究并推广。展开更多
BACKGROUND： As a member of the LIM protein family Ⅱ, cysteine- and glycine-rich protein-2 （CRP2） has been demonstrated to play a role in the regulation of growth and differentiation of eukaryotic cells. Our previo...BACKGROUND： As a member of the LIM protein family Ⅱ, cysteine- and glycine-rich protein-2 （CRP2） has been demonstrated to play a role in the regulation of growth and differentiation of eukaryotic cells. Our previous study has demonstrated that CRP2 can be detected in the embryonic rat inner ear but not in the adult rat inner ear. However, at present, the expression of LIM protein family H members in stem or precursor cells has not been described. OBJECTIVE： To determine the expression and sub-cellular localization of CRP2 in olfactory stem cells. DESIGN, TIME AND SETTING： An experiment with repeated measures was performed in the Laboratory of Otorhinolaryngology, Head and Neck Surgery, Xijing Hospital, the Fourth Military Medical University from February 2008 to April 2008. MATERIALS： Olfactory stem cells, and rabbit-anti-CRP2 polyclonal antibody were prepared and kept in our laboratory. METHODS： Reverse transcription polymerase chain reaction and Western blot analysis were used to detect expression of CRP2 in olfactory stem cells. Immunocytochemistry was also used to localize CRP2 in olfactory stem cells. MAIN OUTCOME MEASURES： The expression and sub-cellular localization of CRP2 in rat olfactory stem cells. RESULTS： CRP2 expression was found in olfactory stem cells, and CRP2 was distributed in both the nucleus and the cytoplasm. CONCLUSION： Confirmation of the expression and distribution of CRP2 in olfactory stem cells.展开更多
Although several considerations have been raised suggesting a beneficial effect of N-acetyl cysteine(NAC)for the treatment of severe acute respiratory syndrome coronavirus 2 infection,there is currently no clinical ev...Although several considerations have been raised suggesting a beneficial effect of N-acetyl cysteine(NAC)for the treatment of severe acute respiratory syndrome coronavirus 2 infection,there is currently no clinical evidence that NAC truly prevents coronavirus disease 2019(COVID-19),reduces the severity of the disease,or improves the outcome.Appropriately designed clinical trials are warranted to prove or disprove a therapeutic effect of NAC for COVID-19 patients.展开更多
文摘目的探讨外周血富含半胱氨酸蛋白61(cysteine-rich protein 61,CCN1)与2型糖尿病患者早期肾损伤的相关性研究。方法回顾性研究河北省石家庄市第二医院2型糖尿病患者321例,以尿液微量白蛋白排泄率(urinary albumin excretion rate,UAER)为金标准,将患者分成将2型糖尿病患者分成早期肾损伤组(n=78,20μg/min≤UAER≤200μg/min)、糖尿病肾病组(n=28,UAER>200μg/min)和正常组(n=215,UAER<20μg/min)。比较3组外周血CCN1表达,Pearson法测定UAER与外周血CCN1的相关性,使用ROC曲线评估外周血CCN1对2型糖尿病早期肾损伤和糖尿病肾病的预测价值。单因素分析基线资料,多因素Logistic回归分析确定早期肾损伤或糖尿病肾病的影响因素。结果糖尿病肾病组CCN1和UAER水平高于早期肾损伤组和正常组;早期肾损伤组的CCN1和UAER水平也高于正常组(P<0.05);外周血CCN1与UAER呈正相关(r=0.916,P<0.001)。以外周血CCN1为检验变量,以是否发生早期肾损伤为状态标量,以敏感度为Y轴,以“1-特异度”为X轴绘制ROC曲线。ROC结果显示,CCN1诊断的临界值为1.59μg/L,其对应的敏感度为85.85%,特异度为73.02%,AUC为0.871(95%CI:0.831~0.903)。以外周血CCN1为检验变量,以是否发生糖尿病肾病为状态标量,以敏感度为Y轴,以“1-特异度”为X轴绘制ROC曲线。ROC结果显示,CCN1诊断的临界值为3.41μg/L,其对应的敏感度为71.43%,特异度为73.72%,AUC为0.761(95%CI:0.707~0.813)。糖尿病肾病组、早期肾损伤组和正常组的病程、高血压、高血脂、收缩压、FPG、糖化血红蛋白(glycated hemoglobin,HbA1c)、尿酸(uric acid,UA)、总胆固醇(total cholesterol,TC)、三酰甘油(triglycerides,TG)和低密度脂蛋白(low-density lipoprotein,LDL)等方面比较差异有统计学意义(P<0.05)。Logistic回归分析结果显示,病程(OR=1.740,95%CI:1.180~2.565)、收缩压(OR=1.943,95%CI:1.267~2.978)、高血脂(OR=1.654,95%CI:1.310~2.088)、FPG(OR=2.776,95%CI:1.260~6.116)、UA(OR=2.228,95%CI:1.174~4.229)和CCN1(OR=3.744,95%CI:1.709~8.200)是2型糖尿病早期肾损伤发生的影响因素(P<0.05)。Logistic回归分析结果显示,病程(OR=1.752,95%CI:1.177~2.609)、收缩压(OR=1.818,95%CI:1.034~3.198)、高血脂(OR=1.680,95%CI:1.320~2.138)、FPG(OR=2.389,95%CI:1.519~3.758)、UA(OR=1.260,95%CI:1.038~1.530)和CCN1(OR=3.815,95%CI:1.636~8.897)是糖尿病肾病发生的影响因素(P<0.05)。结论外周血CCN1与尿液微量白蛋白排泄率呈正相关,是早期肾损伤或糖尿病肾病的危险因素,并可应用于预测早期肾损伤或糖尿病肾病的发生,值得临床进一步研究并推广。
文摘BACKGROUND： As a member of the LIM protein family Ⅱ, cysteine- and glycine-rich protein-2 （CRP2） has been demonstrated to play a role in the regulation of growth and differentiation of eukaryotic cells. Our previous study has demonstrated that CRP2 can be detected in the embryonic rat inner ear but not in the adult rat inner ear. However, at present, the expression of LIM protein family H members in stem or precursor cells has not been described. OBJECTIVE： To determine the expression and sub-cellular localization of CRP2 in olfactory stem cells. DESIGN, TIME AND SETTING： An experiment with repeated measures was performed in the Laboratory of Otorhinolaryngology, Head and Neck Surgery, Xijing Hospital, the Fourth Military Medical University from February 2008 to April 2008. MATERIALS： Olfactory stem cells, and rabbit-anti-CRP2 polyclonal antibody were prepared and kept in our laboratory. METHODS： Reverse transcription polymerase chain reaction and Western blot analysis were used to detect expression of CRP2 in olfactory stem cells. Immunocytochemistry was also used to localize CRP2 in olfactory stem cells. MAIN OUTCOME MEASURES： The expression and sub-cellular localization of CRP2 in rat olfactory stem cells. RESULTS： CRP2 expression was found in olfactory stem cells, and CRP2 was distributed in both the nucleus and the cytoplasm. CONCLUSION： Confirmation of the expression and distribution of CRP2 in olfactory stem cells.
文摘Although several considerations have been raised suggesting a beneficial effect of N-acetyl cysteine(NAC)for the treatment of severe acute respiratory syndrome coronavirus 2 infection,there is currently no clinical evidence that NAC truly prevents coronavirus disease 2019(COVID-19),reduces the severity of the disease,or improves the outcome.Appropriately designed clinical trials are warranted to prove or disprove a therapeutic effect of NAC for COVID-19 patients.
