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5-Bromo-2'-deoxyuridine labeling:historical perspectives,factors infiuencing the detection,toxicity,and its implications in the neurogenesis
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作者 Joaquín Martí-Clúa 《Neural Regeneration Research》 SCIE CAS CSCD 2024年第2期302-308,共7页
The halopyrimidine 5-bromo-2′-deoxyuridine(BrdU)is an exogenous marker of DNA synthesis.Since the introduction of monoclonal antibodies against BrdU,an increasing number of methodologies have been used for the immuno... The halopyrimidine 5-bromo-2′-deoxyuridine(BrdU)is an exogenous marker of DNA synthesis.Since the introduction of monoclonal antibodies against BrdU,an increasing number of methodologies have been used for the immunodetection of this synthesized bromine-tagged base analogue into replicating DNA.BrdU labeling is widely used for identifying neuron precursors and following their fate during the embryonic,perinatal,and adult neurogenesis in a variety of vertebrate species including birds,reptiles,and mammals.Due to BrdU toxicity,its incorporation into replicating DNA presents adverse consequences on the generation,survival,and settled patterns of cells.This may lead to false results and misinterpretation in the identification of proliferative neuroblasts.In this review,I will indicate the detrimental effects of this nucleoside during the development of the central nervous system,as well as the reliability of BrdU labeling to detect proliferating neuroblasts.Moreover,it will show factors influencing BrdU immunodetection and the contribution of this nucleoside to the study of prenatal,perinatal,and adult neurogenesis.Human adult neurogenesis will also be discussed.It is my hope that this review serves as a reference for those researchers who focused on detecting cells that are in the synthetic phase of the cell cycle. 展开更多
关键词 5-bromo-2′-deoxyuridine adult neurogenesis human adult neurogenesis LABELING pitfalls prenatal neurogenesis proliferation S-PHASE suturing S-phase TOXICITY
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Effect of AMPS(2-acrylamido-2-methylpropane sulfonic acid) content on the properties of polymer gels 被引量:2
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作者 Tian-Ci Zhang Ji-Jiang Ge +3 位作者 Hao Wu Hong-Bin Guo Bao-Lei Jiao Zhen Qian 《Petroleum Science》 SCIE CAS CSCD 2022年第2期697-706,共10页
The BST oilfield in the northwestern Taklamakan Desert is a fractured carbonate reservoir,but issues of water breakthrough are becoming increasingly severe with the development of water flooding.Unfortunately,the high... The BST oilfield in the northwestern Taklamakan Desert is a fractured carbonate reservoir,but issues of water breakthrough are becoming increasingly severe with the development of water flooding.Unfortunately,the high-temperature and high-salt conditions(130°C,71695 mg/L)of the BST oilfield pose challenges for the development of plugging agents.In this study,the effects of 2-acrylamido-2-methylpropane sulfonic acid(AMPS)content on AM/AMPS copolymers and gels were studied through viscosity measurements,nuclear magnetic resonance(NMR),and cryo-scanning electron microscope(Cryo-SEM).Moreover,the AMPS stabilization mechanism of the polymers and gels was explained.Heatresistant and salt-tolerant gel systems were developed,and their gelation properties,thermal stability,injection capacity,and plugging ability were evaluated.Experimental results showed inconsistencies between the effects of AMPS content on the polymers and gels.For the polymers,the thermal stability increased with increased AMPS content in the polymer.However,excessive AMPS content resulted in poor gelation and low strength.The developed gel systems with S30 polymer(AMPS content is approximately 26%)exhibited excellent thermal stability,controllable gelation time,good injection capacity,and plugging ability.The field application results indicated that most production wells had a positive response,with reduced water-cut and increased daily oil production. 展开更多
关键词 GELS 2-Acrylamido-2-methylpropane sulfonic acid Enhanced oil recovery HYDROLYSIS SYNERESIS
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Et_3NHCl-AlCl_3 Ionic Liquids as Catalyst for Alkylation of Toluene with 2-Chloro-2-methylpropane 被引量:2
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作者 Chen Han Luo Guohua +2 位作者 Xu Xin Wang Yanli Xia Jiajia 《China Petroleum Processing & Petrochemical Technology》 SCIE CAS 2013年第1期54-60,共7页
Alkylation of toluene With 2-chloro-2-methylpropane (t-Bu-C1) to synthesize para-tert-butyltoluene (PTBT) was carded out in the presence of triethylamine hydrochloride-aluminum chloride ionic liquids used as the c... Alkylation of toluene With 2-chloro-2-methylpropane (t-Bu-C1) to synthesize para-tert-butyltoluene (PTBT) was carded out in the presence of triethylamine hydrochloride-aluminum chloride ionic liquids used as the catalyst. The ionic liquids were prepared with different molar ratios of Et3NHC1 to A1CI3, and the effect of the molar ratio between A1C13 and Et3NHC1, the reaction time, the reaction temperature, the ionic liquid dosage, as well as the molar ratio of toluene to chloro- 2-methylpropane on the alkylation reaction of toluene with chloro-2-methyl-propane was investigated. The test results showed that the acidic ionic liquids prepared with Et3NHC1 and A1C13 had good activity and selectivity for the alkylation reaction of toluene with alkyl chloride to produce PTBT. The optimal reaction conditions were specified at an A1C13 to Et3N- HCI ratio of 1.6, a reaction temperature of 20 ℃, a mass fraction of toluene to ionic liquid of 10%, and a chloro-2-methyl- propane to toluene molar ratio of 0.5. Under the suitable reaction conditions, a 98% conversion of chloro-2-methylpropane and an 82.5% selectivity of PTBT were obtained. Ionic liquids could be reused 5 times with its catalytic activity unchanged, and the regenerated ionic liquids can be recycled. 展开更多
关键词 ALKYLATION ionic liquid TOLUENE 2-chloro-2-methylpropane EhNHC1-A1C13
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Kinetic, electronic properties and conductivity studies of ((12Z)N-(5Z)-1-bromo-5-(phenylimino)-1H-pyrrol-2(5H)-ylidene benzenmine {ArN(CNBrCCHCH)NAr} (Ar = C6H5) 被引量:1
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作者 Salem Et. Ashoor Noori Y. Salman 《Journal of Chemistry and Chemical Engineering》 2009年第10期51-56,共6页
Kinetic and conductivity of new Schiff bases compounds such ((12Z)N-(5Z)-1-bromo-5-(phenylimino)-1H- pyrrol-2(5 H)-ylidene)benzenamine {ArN(CNBrCCHCH)NAr} (Ar = C6H5) incorporating the chelating diamino ... Kinetic and conductivity of new Schiff bases compounds such ((12Z)N-(5Z)-1-bromo-5-(phenylimino)-1H- pyrrol-2(5 H)-ylidene)benzenamine {ArN(CNBrCCHCH)NAr} (Ar = C6H5) incorporating the chelating diamino has been studied in this paper and was found a second order of this reaction. Electronic Properties using hyperchem program study has been improved for this compound such angel and bond distance, and then this compound was defining as electric conductivity and did prove to be useful for conduclively compound. 展开更多
关键词 Schiff bases compounds ((12Z)N-(5Z)-1-bromo-5-(phenylimino)- 1H-pyrrol-2(5H)-ylide ne)benzenamine chelating diamino: electronic properties
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Optical Resolution ofα-Bromo-2-Chlorophenacetic Acid by Way of Coordination with Copper(II) and O,O’-Dibenzoyltartaric Acid
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作者 Xu HongWu Wang QiWei +3 位作者 Deng JinGen Zhu Jin Xu XinLiang Wu YuLiang 《合成化学》 CAS CSCD 2004年第z1期34-34,共1页
关键词 Resolution α-bromo-2-chloro-phenacetic acid asymmetric transformation DBTA
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Synthesis, Structure and Geometrical Calculation of a Novel Co-crystal of {[4-Bromo-2-(benzimidazol-2-yl)phenolato][2-(1-butylbenzimidazol-2-yl)phenolato]zinc(Ⅱ)] and Bis[μ-2-(1-butylbenzimidazol-2-yl)phenolato]-1κN^3:2κO;1κO:2κN^3-bis{[2-(1-butylbe
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作者 TONG Yi-Ping 《Chinese Journal of Structural Chemistry》 SCIE CAS CSCD 北大核心 2007年第2期143-148,共6页
The title compound has been synthesized and characterized crystallographicaUy. It is a co-crystal consisting of two different neutral zinc(H) complexes with Hbpbm (Hbpbm = 4- bromo-2-(benzimidazol-2-yl)phenol) a... The title compound has been synthesized and characterized crystallographicaUy. It is a co-crystal consisting of two different neutral zinc(H) complexes with Hbpbm (Hbpbm = 4- bromo-2-(benzimidazol-2-yl)phenol) and Hnpbm (Hnpbm = 2-(1-butylbenzimidazol-2-yl)phenol). One is a monomeric mixed-ligand complex of [Zn(bpbm)(npbm)] 1 and the other a dimer of [Zn2(npbm)4] 2 with their ratio of 2:1. Thus the overall formula for the title compound is 21.2. Adjacent 1 and 2 are connected to each other by intermolecular hydrogen bonding interactions in the lattice. The crystal data: monoclinic, space group P21/c, a = 15.0141(12), b = 20.9941(17), c = 18.4686(15) A, β = 97.445(2)°, V = 5772.4(8) A^3, Mr= 2429.68, Z = 2, Dc = 1.398 g/cm^3,μ = 1.579 -1 mm , F(000) = 2504, R = 0.0637 and wR = 0.1771 for 6464 observed reflections (I 〉 2σ(I)). The geometrical structure for 1 has also been theoretically optimized and compared with the experimental one. 展开更多
关键词 zinc complex co-crystal structure 4-bromo-2-(1-butylbenzimidazol-2-yl)phenol 2-(1-butylbenzimidazol-2-yl)phenol
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Coupling reaction mechanism of 2-(2-bromo-4-fluorophenyl)-1-cyclohexen-1-yl trifluoromethane-sulfonate
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作者 白坤坤 YOU Yong +2 位作者 PAN Rui 李来才 TIAN An-min 《Journal of Chongqing University》 CAS 2013年第3期108-116,共9页
The detailed mechanism of CuI-catalyzed C-O intramolecular coupling reaction of 2-(2-bromo-4-fluoro-phenyl)-l- cyclohexen-1-yl trifluoromethane-sulfonate was studied with the density functional theory (DFT). The g... The detailed mechanism of CuI-catalyzed C-O intramolecular coupling reaction of 2-(2-bromo-4-fluoro-phenyl)-l- cyclohexen-1-yl trifluoromethane-sulfonate was studied with the density functional theory (DFT). The geometries of the reactants, transition states, intermediates and products were optimized at the B3LYP/6-31 +G* level. Meanwhile, the single point energy of species involved in gas and solvent at B3LYP/6-31 I+G* level was individually investigated. Polarizable continuum models (PCM) were applied to the dioxane and water solutions at the same level, respectively. Results show that the rate-limiting step, M3→TS3, does not change in different solutions. However, the activation energy in a dioxane solution is lower than that in water, which explains the previous experimental results. Compared with the non-catalyzed reaction process, the activation energy of the rate- limiting step is reduced by 56.53 kJ mo1-1 in gas and 44.84 kJ mol-l in solvent, demonstrating a high catalytic efficiency of CuI. 展开更多
关键词 2-(2-bromo-4-fluoro-phenyl)-l-cyclohexen-l-yl trifluoromethane-sulfonate CuI density functional theory Calculation polarizable continuum model coupling reaction
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Theoretical Study on the Reaction Mechanism of 2-Methoxybenzaldehyde,4-Bromo-indanone,Malononitrile and Ammonium Acetate One-pot to Form 6-(2-Methoxyphenyl)-2-amino-6-bromo-5H-indeno[1,2-b]pyridine-3-carbonitrile
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作者 张福兰 黄辉胜 《Chinese Journal of Structural Chemistry》 SCIE CAS CSCD 2017年第10期1685-1696,共12页
The reaction mechanism of 2-methoxybenzaldehyde, 4-bromo-indanone, malononitrile and ammonium acetate one-pot to form 6-(2-methoxyphenyl)-2-amino-6-bromo-5 Hindeno[1,2-b]pyridine-3-carbonitrile was studied by densit... The reaction mechanism of 2-methoxybenzaldehyde, 4-bromo-indanone, malononitrile and ammonium acetate one-pot to form 6-(2-methoxyphenyl)-2-amino-6-bromo-5 Hindeno[1,2-b]pyridine-3-carbonitrile was studied by density functional theory. The geometries of the reactants, transition states, intermediates and products were optimized at the PW91/DNP level. Vibration analysis was carried out to confirm the transition state structure. Reaction pathways were investigated in this study. The result indicates that the reaction Re→ TSB1→IMB1→ TSB2→ IMB2→TSB3→IMB3→TSB4→IMB4→TSB5→IMB5→TSB6→IMB6→TSB7→IMB7→ TSB8→IMB8→TSB9→IMB9→P2 is the main pathway, the activation energy of which is the lowest. The dominant product predicted theoretically is in agreement with the experiment results. 展开更多
关键词 2-methoxybenzaldehyde 4-bromo-indanone 6-(2-methoxyphenyl)-2-amino-6-bromo-5H-indeno[1 2-b]pyridine-3-carbonitrile density functional reaction mechanism
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Synthesis,Crystal Structure and Anticancer Property of (Z)-N-(4-Bromo-5-ethoxy-3,5-dimethyl-furan-2(5H)-ylidene)-4-methylbenzenesulfonamide
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作者 张世杰 胡惟孝 《Chinese Journal of Structural Chemistry》 SCIE CAS CSCD 2010年第8期1275-1279,共5页
The novel title compound(Z)-N-(4-bromo-5-ethoxy-3,5-dimethylfuran-2(5H)-yli-dene)-4-methylbenzenesulfonamide 1(C15H18BrNO4S) has been unexpectedly synthesized by the aminohalogenation reaction of ethyl 2-methy... The novel title compound(Z)-N-(4-bromo-5-ethoxy-3,5-dimethylfuran-2(5H)-yli-dene)-4-methylbenzenesulfonamide 1(C15H18BrNO4S) has been unexpectedly synthesized by the aminohalogenation reaction of ethyl 2-methylpenta-2,3-dienoate with TsNBr2,and characterized by mp,IR,1H NMR,EIMS,ESIHRMS and single-crystal X-ray diffraction.It crystallizes in mono-clinic,space group P21/c with a = 11.714(5),b = 14.106(5),c = 10.402(4) ,β = 97.298(8)°,V = 1704.9(12) 3,Mr = 388.27,Z = 4,Dc = 1.513 g/cm3,μ(MoKα) = 2.549 mm-1,F(000) = 792,the final R = 0.033 and wR = 0.062 for 3098 observed reflections(Ⅰ 〉 2σ(Ⅰ)). 展开更多
关键词 synthesis crystal structure N-(4-bromo-5-ethoxy-3 5-dimethylfuran-2(5H)-ylidene)-4-methylbenzenesulfonamide ANTICANCER
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Synthesis, Crystal Structure and Biological Activities of 3-Bromo-5-(4-chlorophenyl)-4-cyanopyrrole-N,N-dimethyl-2-carboxamide
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作者 许庆博 华云涛 +3 位作者 唐强 周宝晗 陈坤 徐保明 《Chinese Journal of Structural Chemistry》 SCIE CAS CSCD 2018年第5期747-752,共6页
The title compound 3-bromo-5-(4-chlorophenyl)-4-cyanopyrrole-N,N-dimethyl-2-amide(3) was synthesized with 4-bromo-2-(4-chlorophenyl)-5-(trifluoromethyl)-1H-pyrrole-3-carbonitrile(1) and N,N-dimethylformamide... The title compound 3-bromo-5-(4-chlorophenyl)-4-cyanopyrrole-N,N-dimethyl-2-amide(3) was synthesized with 4-bromo-2-(4-chlorophenyl)-5-(trifluoromethyl)-1H-pyrrole-3-carbonitrile(1) and N,N-dimethylformamide(2) by the α-C acylation reaction catalyzed by potassium t-butoxide, and characterized by IR, 1H-NMR and X-ray single-crystal diffraction. It crystallizes in monoclinic, space group P2(1/n)with a = 12.789(2), b = 13.783(2), c = 17.980(3) °, β = 109.230(3)°, V = 2992.5 A3, Mr = 352.62, Dc = 1.565 mg/m3, Z = 8, m = 2.924 mm-1, F(000) = 1408, the final R = 0.0424 and w R = 0.0973 for 3518 observed reflections with I 〉 2σ(I). A total of 23559 reflections were collected, of which 6242 were independent(Rint = 0.0566). The insecticidal, herbicidal and antibacterial activities of compound 3 were determined, and the experimental results showed that the mortality of 3 at the concentration of 100 ppm on the Fipronil against Linnaeus was 76.6%, the growth inhibition rate of 3 against Cynodon Dactylon under the condition of 100 ppm was 35.8% and the inhibitory activity of 3 at the concentration of 25 ppm against Fusarium graminearum reached 50.9%. Hence, the title compound has the value of further research and application prospect. 展开更多
关键词 3-bromo-5-(4-chlorophenyl)-4-cyanopyrrole-N N-dimethyl-2-amide SYNTHESIS crystal structure biological activity
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(Z)-ETHYL 3-BROMO-4,4,4-TRIFLUORO-2-BUTENOATE AS A NOVEL CF_3-CONTAINING BUILDING BLOCK: Its Preparation and Pd-Catalysed Reaction with Alkynes and Organozinc Reagents
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作者 Wei Yuan HUANG, Long Lǔ Shanghai Institute of Organic Chemistry, The Chinese Academy of Sciences, 345 Lingling Lu, Shanghai, 200032 《Chinese Chemical Letters》 SCIE CAS CSCD 1991年第10期769-772,共4页
A novel CF_3-containing building block, (Z)-ethyl 3-bromo-4,4,4-trifluoro-2-butenoate, was easily prepared from CF_3CBr_3, the former reacted with alkynes Or organozinc reagents in the presence of Pd complexes to affo... A novel CF_3-containing building block, (Z)-ethyl 3-bromo-4,4,4-trifluoro-2-butenoate, was easily prepared from CF_3CBr_3, the former reacted with alkynes Or organozinc reagents in the presence of Pd complexes to afford useful CF_3-containing intermediates in good yield. 展开更多
关键词 CF Br ETHYL 3-bromo-4 4 4-TRIFLUORO-2-BUTENOATE AS A NOVEL CF3-CONTAINING BUILDING BLOCK Its Preparation and Pd-Catalysed Reaction with Alkynes and Organozinc Reagents PD AS
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Synthesis and Crystal Structure of N1-(2-(4-Bromo-2-nitrophenoxy)-2-dimethyl acyloxymethyl)-5-fluorouracil
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作者 贾忠 李靖 +5 位作者 张阳 赵静思 席娜 胡春红 胡敏敏 贺殿 《Chinese Journal of Structural Chemistry》 SCIE CAS CSCD 2016年第7期1047-1053,共7页
The title compound N1-(2-(4-bromo-2-nitrophenoxy)-2-dimethyl acyloxymethyl)-5-fluorouracil(C15H13BrFN3O7, Mr = 446.19) was synthesized and structurally characterized by ^1H-NMR, -(13)C-NMR, ESI-MS and single-c... The title compound N1-(2-(4-bromo-2-nitrophenoxy)-2-dimethyl acyloxymethyl)-5-fluorouracil(C15H13BrFN3O7, Mr = 446.19) was synthesized and structurally characterized by ^1H-NMR, -(13)C-NMR, ESI-MS and single-crystal X-ray diffraction. The compound crystallizes in triclinic, space group P1, with a = 8.3325(6), b = 10.1808(13), c = 11.8194(16) A, α = 73.194(12), β= 72.351(9), γ = 89.509(8)o, V = 911.2(2) A^3, Z = 2, T = 300.79(10) K, μ(Cu Kα) = 3.578 mm^-1, Dc = 1.626 g/cm3, F(000) = 448.0, GOF = 1.060, 5723 reflections measured(8.232≤2θ≤139.592°), 3339 unique(Rint = 0.0184, Rsigma = 0.0254) which were used in all calculations. The final R = 0.0517(I 〉 2s(I)) and wR = 0.1494(all data). Hydrogen bonds and π-π interactions together stabilize the structure of the molecule. The preliminary biological test shows that the title compound has good antitumor activity against A549 in vitro and lower toxicity to normal cells. 展开更多
关键词 crystal structure synthesis N1-(2-(4-bromo-2-nitrophenoxy)-2-dimethyl acyloxymethyl)-5-fluorouracil antitumor activity
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Synthesis and Crystal Structure of 4-Bromo-5-ethoxy-3-methyl-5- (naphthalen-1-yl)-1-tosyl-1H-pyrrol-2(5H)-one
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作者 沈如伟 杨誉竹 +2 位作者 曹剑 吴露玲 黄宪 《Chinese Journal of Structural Chemistry》 SCIE CAS CSCD 北大核心 2007年第12期1505-1508,共4页
The title compound 4-bromo-5-ethoxy-3-methyl-5-(naphthalen-l-yl)-l-tosyl-lH- pyrrol-2(5H)-one 1 (C24H22BrNO4S, Mr = 500.40) has been synthesized and its crystal structure was determined by single-crystal X-ray d... The title compound 4-bromo-5-ethoxy-3-methyl-5-(naphthalen-l-yl)-l-tosyl-lH- pyrrol-2(5H)-one 1 (C24H22BrNO4S, Mr = 500.40) has been synthesized and its crystal structure was determined by single-crystal X-ray diffraction analysis. It crystallizes in monoclinic, space group P21/n with a = 8.8562(15), b = 18.118(3), c = 14.055(2)A, β = 99.855(3)^o, V= 2221.9(6)A3, Z = 4, Dc = 1.496 g/cm^3,μ= 1.975 mm^-1, 2 = 0.71073A, F(000) = 1024, R = 0.0607 and wR = 0.1371. 展开更多
关键词 4-bromo-5-ethoxy-3-methyl-5-(naphthalen-1-y1)-1-tosyl-1H-pyrrol-2(5H)-one synthesis crystal structure
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Synthesis and Structural Characterization of Organophosphorus Ylide-1-(3-nitrophenyl)-2-(triphenyl phosphoranylidene)ethanone 被引量:1
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作者 ALIREZA Dadrass ALI Ramazani +6 位作者 AHMAD Poursattar Marjani HOJJATOLLAH Rahchamani HASSAN Nasri Koureh ALI Souldozi SEPIDEH Samiee KATARZYNA lepokura TADEUSZ Lis 《Chinese Journal of Structural Chemistry》 SCIE CAS CSCD 2015年第3期373-378,共6页
The title compound Ph3P=C(H)C(O)PhNO2 (L) has been prepared by the addition of triphenylphosphine in acetone as solvent to 2-bromo-1-(3,nitrophenyl)ethanone followed by the addition of alkaline solution of sod... The title compound Ph3P=C(H)C(O)PhNO2 (L) has been prepared by the addition of triphenylphosphine in acetone as solvent to 2-bromo-1-(3,nitrophenyl)ethanone followed by the addition of alkaline solution of sodium hydroxide. The yellow crystals of the title ylide were grown in methanol/chloroform solution by drop method without stirring at room temperature. The solid state structure of ylide has been established by X-ray crystallography analysis. In the molecule of the title compound, the geometry around the P atom is nearly tetrahedral and the O atom is in synperiplanar orientation to the P atom. The nitrophenyl ring is twisted with respect to the plane of the carbonyl group through an angle of 36.6(1)°. The crystal (C26H20NO3P, Mr = 425.40) belongs to the monoclinic system, space group P21/n with a = 10.889(3), b = 14.467(3), e = 13.872(4)A, β = 103.08(3)°, V = 2128.6(10) A3, Z= 4, T= 100(2) K, R = 0.059 and wR = 0.163 for 3984 observed reflections with I〉 2σ(I). 展开更多
关键词 crystal structure phosphorus yUde 2-bromo-1-(3-nitrophenyl)ethanone triphenylphosphine
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Preconcentration/Separation of Some Metal Ions Using Sodium Dodecyl Sulfate Coated Alumina Modified with Bis(5-bromo- 2-hydroxy-benzaldehyde)-2-methyl-1,5-pentane Diimine (BBHBPDI) Prior to Their Flame-AAS Determination
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作者 Ghaedi, Mehrorang Montazerozohori, Mortez +2 位作者 Marahel, Farzaneh Nejati Biareh, Mehdi Soylak, Mostafa 《Chinese Journal of Chemistry》 SCIE CAS CSCD 2011年第10期2141-2147,共7页
Preconcentration/separation of Co(II), Fe(III), Pb(II), Cr(IIl), Cu(II) and Cd(II) ions using bis(5-bromo- 2-hydroxy-benzaldehyde)-2-methy1-1,5-pentane diimine (BBHBPDI) on SDS coated alumina has been ... Preconcentration/separation of Co(II), Fe(III), Pb(II), Cr(IIl), Cu(II) and Cd(II) ions using bis(5-bromo- 2-hydroxy-benzaldehyde)-2-methy1-1,5-pentane diimine (BBHBPDI) on SDS coated alumina has been reported. The influences of the analytical parameters including pH, ligand and SDS amount, type and concentration of eluent and sample volume on metal ions recoveries were investigated. At optimum values of all variables the relative standard deviation are between 2.5-2.7 and preconcentration factor was 375, while recoveries for all understudy metal ions are higher than 95%, determination limits are between 1.5-2.7. The method has been successfully applied to determination of Co(II), Fe(III), Pb(II), Cr(III), Cu(II) and Cd(II) ions content in some real samples. 展开更多
关键词 surfactant coated alumina atomic absorption spectrometry solid phase extraction bis(5-bromo-2-hydroxy-benzaldehyde)-2-methyl- 1 5-pentane diimine 3 - [(1H-indo1-3y1)- 3 4 5-trimethyl] - 1H-indole
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豚鼠前庭椭圆囊器官在庆大霉素损伤后离体培养的细胞增殖
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作者 孟曦曦 郑少鹏 +2 位作者 刘鋌 王元身 赵春礼 《中华耳科学杂志》 CSCD 2003年第2期1-4,共4页
目的 在离体状态下对豚鼠前庭椭圆囊器官进行培养,观察庆大霉素损伤毛细胞后细胞的增殖情况。方法采用白色健康豚鼠36只,体重300-450克,随机分为4组:(1)正常对照组;(2)正常加BrdU(5-溴基脱氧尿核苷BrdU)组;(3)庆大霉素组;(4)庆大霉素加B... 目的 在离体状态下对豚鼠前庭椭圆囊器官进行培养,观察庆大霉素损伤毛细胞后细胞的增殖情况。方法采用白色健康豚鼠36只,体重300-450克,随机分为4组:(1)正常对照组;(2)正常加BrdU(5-溴基脱氧尿核苷BrdU)组;(3)庆大霉素组;(4)庆大霉素加BrdU组。应用体外组织培养、5—溴基脱氧尿核苷(BrdU,3μg/ml)掺入及免疫组织化学染色和Epon树酯包埋半薄切片等方法,在不同时间(2、3、5、15天)光镜观察庆大霉素(2.0mM)损伤后毛细胞及支持细胞增殖情况。结果 用庆大霉素48小时后,可见到椭圆囊感觉上皮中的毛细胞溶解破坏。加入BrdU继续培养,BrdU免疫组织化学染色显示了第5和15天被BrdU标记的阳性细胞核,阳性细胞核多位于椭圆囊感觉上皮中基底层,从细胞的形态和分布位置上看为支持细胞;感觉上皮的表层,也可观察到BrdU反应阳性的上皮细胞,细胞核较圆,形态和位置类似毛细胞。结论 提示损伤后的椭圆囊感觉上皮支持细胞可以进行细胞的有丝分裂,支持细胞可能是修复毛细胞的前体细胞。 展开更多
关键词 椭圆囊 增殖细胞 体外 庆大霉素 5-溴基脱氧尿核苷(5-bromo-2'-deoxyuridine BrdU)
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Effects of Repetitive Transcranial Magnetic Stimulation on Astrocytes Proliferation and nNOS Expression in Neuropathic Pain Rats 被引量:10
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作者 Lu YANG Sai-hua WANG +3 位作者 Yan HU Yan-fang SUI Tao PENG Tie-cheng GUO 《Current Medical Science》 SCIE CAS 2018年第3期482-490,共9页
This study investigated the effects of different frequencies of repetitive transcranial magnetic stimulation (rTMS) on chronic neuropathic pain in rats. The behavior of rats with experimental chronic neuropathic pai... This study investigated the effects of different frequencies of repetitive transcranial magnetic stimulation (rTMS) on chronic neuropathic pain in rats. The behavior of rats with experimental chronic neuropathic pain was observed, and the expression of neuronal nitric oxide synthase (nNOS) in the ipsilateral dorsal root ganglions (DRGs) and the activation and proliferation of astrocytes in the ipsilateral spinal dorsal horn were detected. Thirty-two male Sprague-Dawley rats were randomly divided into four groups: sham-operated group, sham-rTMS group, 1 Hz group and 20 Hz group (8 rats in each group). Chronic constriction nerve injury induced by sciatic nerve ligation was made to establish the models of the chronic neuropathic pain in rats except those in the sham-operated group. Then we applied different frequencies of rTMS to the primary motor cortex (M1) contralateral to the pain side once daily for 10 consecutive days. Pain behavior scores were observed before and after treatment. Western blot analysis was used to detect the expression of nNOS in ipsilateral L4-6 DRGs. Double immunofluorescent labeling for glial fibrillary acidic protein (GFAP) and 5-bromo-2- deoxyuridine (BrdU) was employed to observe the activation and proliferation of astrocytes in the ipsilateral L4-6 spinal dorsal horn. After rTMS treatment, the spontaneous pain behavior scores were significantly lower in the 20 Hz group than those in the sham-rTMS group (P〈0.05). Moreover, the brush-evoked pain behavior scores were significantly lower in the 20 Hz group than those in the sham-rTMS and 1 Hz group (P〈0.05), suggesting that the spontaneous pain and brush-evoked pain in the 20 Hz group were significantly alleviated. Western blot analysis revealed that the expression of nNOS in ipsilateral L4-6 DRGs was significantly decreased in the 20 Hz group as compared with the sham-rTMS group and the 1 Hz group (P〈0.01) after rTMS treatment. Double immunofluorescence suggested that the expression of GFAP and the co-localization with BrdU in astrocytes were less in the sham-operated group than those in the sham-rTMS group and the 1 Hz group in L4-6 spinal dorsal horn ipsilateral to the neuropathic pain. After rTMS treatment, the expression of GFAP and the co-localization with BrdU decreased in the 20 Hz group as compared with the sham-rTMS group and the 1 Hz group (P〈0.05). In addition, the alleviation degree of spontaneous pain and brush-evoked pain in the 20 Hz group was negatively correlated with the expression of nNOS in ipsilateral DRGs and the number of GFAP/BrdU co-labelled astrocytes in L4-6 spinal dorsal horn ipsilateral to the neuropathic pain (P〈0.05). It was suggested that high-frequency rTMS may relieve neuropathic pain through down-regulating the overexpression of nNOS in ipsilateral DRGs and inhibiting the activity and proliferation of astrocytes in L4-6 spinal dorsal horn ipsilateral to the neuropathic pain. 展开更多
关键词 neuropathic pain repetitive transcranial magnetic stimulation neuronal nitric oxide synthase ASTROCYTE glial fibrillary acidic protein 5-bromo-2-deoxyuridine
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Proliferation and Differentiation of Duct Epithelial Cells after Partial Pancreatectomy in Rats 被引量:3
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作者 刘涛 王春友 +2 位作者 万赤丹 熊炯炘 周峰 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2006年第5期567-569,共3页
The proliferation and differentiation of pancreatic duct epithelial cells in remnant pancreas during regeneration after partial pancreatectomy in rats were studied, and the source of pancreatic stem cells was characte... The proliferation and differentiation of pancreatic duct epithelial cells in remnant pancreas during regeneration after partial pancreatectomy in rats were studied, and the source of pancreatic stem cells was characterized. Partial (90 %) pancreatectomy was performed on 4- to 5-week-old Sprague-Dawley rats, and different duct epithelial cells and acinar cells were detected by immunohistrochemical stain method and scored using 5-bromo-2'-deoxyuridine (BrdU) labeling index (LI) at various time points after partial pancreatectomy. It was found that at 24 h after partial pancreatectomy proliferation started in the main, large and small duct cells, and persisted in small duct cells to day 5. There was significant difference between the experimental group and the control group (P〈0.001). Acinar cells positive for BrdU were greatly increased and reached the peak LI on day 5. The destroyed lobular architecture almost totally recovered on day 7, and the newly islet cells appeared around the pancreatic ducts. These results suggest that regeneration after partial pancreatectomy is involved in proliferation and differentiation of pancreatic stem cells, and pancreatic stem cells may locate in the pancreatic ductules. 展开更多
关键词 PANCREAS stem cells REGENERATION 5-bromo-2'-deoxyuridine RATS
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Synthesis and Characterization of a Novel Cellulose Nonionic Ether 被引量:2
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作者 邵自强 徐坤 +2 位作者 田永生 王飞俊 王继勋 《Journal of Beijing Institute of Technology》 EI CAS 2005年第2期188-191,共4页
A kind of novel cellulose ether-trihydroxybutyl cellulose (THBC) was synthesized. The process includes the steam explosion treatment of cotton cellulose, alkalization, etherification and purification. Sweep electron m... A kind of novel cellulose ether-trihydroxybutyl cellulose (THBC) was synthesized. The process includes the steam explosion treatment of cotton cellulose, alkalization, etherification and purification. Sweep electron microscope (SEM), Fourier transform infrared (FTIR) and X-ray diffraction were used to characterize the cellulose pretreated and the product. The effects of reaction conditions (temperature, time) on the molecular substitution (S_m) were discussed. To obtain a higher degree of molecular substitution, the reaction temperature is 80 ℃, and the reaction time is 4 h. 展开更多
关键词 steam explosion trihydroxybutyl cellulose THERMOPLASTIC 3 4-epoxy-1-bromo-2-butyl alcohol
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Unexpected BrdU inhibition on astrocyte-to-neuron conversion 被引量:2
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作者 Tao Wang Jian-Cheng Liao +7 位作者 Xu Wang Qing-Song Wang Kai-Ying Wan Yi-Yi Yang Qing He Jia-Xuan Zhang Gong Chen Wen Li 《Neural Regeneration Research》 SCIE CAS CSCD 2022年第7期1526-1534,共9页
5-Bromo-2′-deoxyuridine(BrdU)is a halogenated pyrimidine that can be incorporated into newly synthesized DNA during the S phase of the cell cycle.BrdU is widely used in fate-mapping studies of embryonic and adult neu... 5-Bromo-2′-deoxyuridine(BrdU)is a halogenated pyrimidine that can be incorporated into newly synthesized DNA during the S phase of the cell cycle.BrdU is widely used in fate-mapping studies of embryonic and adult neurogenesis to identify newborn neurons,however side effects on neural stem cells and their progeny have been reported.In vivo astrocyte-to-neuron(AtN)conversion is a new approach for generating newborn neurons by directly converting endogenous astrocytes into neurons.The BrdU-labeling strategy has been used to trace astrocyte-converted neurons,but whether BrdU has any effect on the AtN conversion is unknown.Here,while conducting a NeuroD1-mediated AtN conversion study using BrdU to label dividing reactive astrocytes following ischemic injury,we accidentally discovered that BrdU inhibited AtN conversion.We initially found a gradual reduction in BrdU-labeled astrocytes during NeuroD1-mediated AtN conversion in the mouse cortex.Although most NeuroD1-infected astrocytes were converted into neurons,the number of BrdU-labeled neurons was surprisingly low.To exclude the possibility that this BrdU inhibition was caused by the ischemic injury,we conducted an in vitro AtN conversion study by overexpressing NeuroD1 in cultured cortical astrocytes in the presence or absence of BrdU.Surprisingly,we also found a significantly lower conversion rate and a smaller number of converted neurons in the BrdU-treated group compared with the untreated group.These results revealed an unexpected inhibitory effect of BrdU on AtN conversion,suggesting more caution is needed when using BrdU in AtN conversion studies and in data interpretation. 展开更多
关键词 5-bromo-2′-deoxyuridine NeuroD1 astrocyte-to-neuron conversion reprogramming neural regeneration reactive astrocytes neurons lineage tracing fate mapping neural stem cell
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