[Objectives]To investigate whether paeonol(Pae)combined with panax notoginseng saponins(PNS)can protect the myocardium of rats with diabetic cardiomyopathy(DCM)through improving the antioxidant capacity of the rats by...[Objectives]To investigate whether paeonol(Pae)combined with panax notoginseng saponins(PNS)can protect the myocardium of rats with diabetic cardiomyopathy(DCM)through improving the antioxidant capacity of the rats by activating the Nrf2/ARE pathway.[Methods]The rats were fed with high-fat and high-sugar diet for 6 weeks,and combined with intraperitoneal injection of small-dose STZ to build a type II diabetes model;the model rats were randomly divided into a model group,a Pae group of 80 mg/kg,and a PNS group of 100 mg/kg,a Pae 80 mg/kg+PNS 100 mg/kg,and a metformin group 157.5 mg/kg;rats of normal group and model group were injected with an equal volume of sodium carboxymethyl cellulose(1%),10 rats in each group.After 8 weeks,3 rats in the model group were taken for histopathological examination.Changes in myocardial fibrosis and myocardial collagen formation indicated that the building of DCM model was successful.qRT-PCR and Western blot were used to detect the expression levels of Nrf2,HO-1,Col-I,and Col-III in myocardial tissue of each group of rats.[Results]Compared with the normal group,the mRNA and protein expression levels of Nrf2 and HO-1 in myocardial tissue of the DCM group rats were significantly reduced,and the mRNA and protein expression levels of Col-I and Col-III were significantly increased;compared with the DCM group,the mRNA and protein expression levels of Nrf2 and HO-1 in the myocardial tissue of each drug group increased to varying degrees,and the combined drug group increased more significantly than that of the single drug group;the mRNA and protein expression levels of Col-I and Col-III were reduced to varying degrees,and the combined drug group declined more significantly than that of the single drug group.[Conclusions]Paeonol combined with panax notoginseng saponins can up-regulate the expression of Nrf2 and HO-1 in myocardial tissue,inhibit the expression of type I and type III collagen.The mechanism may be related to improving the DCM myocardial fibrosis through activating the Nrf2/ARE pathway.展开更多
Two new dammarane glycosides named notoginsenoside T-1 and T-2 were isolated from the mild acid hydrolysis products of the root saponins of Panax notoginseng. On the basis of spectroscopic evidences, their structures ...Two new dammarane glycosides named notoginsenoside T-1 and T-2 were isolated from the mild acid hydrolysis products of the root saponins of Panax notoginseng. On the basis of spectroscopic evidences, their structures were elucidated to be 6-O-beta -D-glucopyranosyl-24(25)-epoxy-3 beta ,6 alpha ,12 beta ,23 xi -tetrahydroxydammar-20(22)(E)-ene 1 and 6-O-beta -D-glucopyranosyl-24(25)-epoxy-23 xi -methoxyl-3 beta ,6 alpha ,12 beta -trihydroxydamm-ar-20(22)(E)-ene 2, respectively.展开更多
文摘[Objectives]To investigate whether paeonol(Pae)combined with panax notoginseng saponins(PNS)can protect the myocardium of rats with diabetic cardiomyopathy(DCM)through improving the antioxidant capacity of the rats by activating the Nrf2/ARE pathway.[Methods]The rats were fed with high-fat and high-sugar diet for 6 weeks,and combined with intraperitoneal injection of small-dose STZ to build a type II diabetes model;the model rats were randomly divided into a model group,a Pae group of 80 mg/kg,and a PNS group of 100 mg/kg,a Pae 80 mg/kg+PNS 100 mg/kg,and a metformin group 157.5 mg/kg;rats of normal group and model group were injected with an equal volume of sodium carboxymethyl cellulose(1%),10 rats in each group.After 8 weeks,3 rats in the model group were taken for histopathological examination.Changes in myocardial fibrosis and myocardial collagen formation indicated that the building of DCM model was successful.qRT-PCR and Western blot were used to detect the expression levels of Nrf2,HO-1,Col-I,and Col-III in myocardial tissue of each group of rats.[Results]Compared with the normal group,the mRNA and protein expression levels of Nrf2 and HO-1 in myocardial tissue of the DCM group rats were significantly reduced,and the mRNA and protein expression levels of Col-I and Col-III were significantly increased;compared with the DCM group,the mRNA and protein expression levels of Nrf2 and HO-1 in the myocardial tissue of each drug group increased to varying degrees,and the combined drug group increased more significantly than that of the single drug group;the mRNA and protein expression levels of Col-I and Col-III were reduced to varying degrees,and the combined drug group declined more significantly than that of the single drug group.[Conclusions]Paeonol combined with panax notoginseng saponins can up-regulate the expression of Nrf2 and HO-1 in myocardial tissue,inhibit the expression of type I and type III collagen.The mechanism may be related to improving the DCM myocardial fibrosis through activating the Nrf2/ARE pathway.
基金the members of analytic group of Laboratory of Phytochemistry, Kunming Institute of Botany, Chinese Academy of Sciences.
文摘Two new dammarane glycosides named notoginsenoside T-1 and T-2 were isolated from the mild acid hydrolysis products of the root saponins of Panax notoginseng. On the basis of spectroscopic evidences, their structures were elucidated to be 6-O-beta -D-glucopyranosyl-24(25)-epoxy-3 beta ,6 alpha ,12 beta ,23 xi -tetrahydroxydammar-20(22)(E)-ene 1 and 6-O-beta -D-glucopyranosyl-24(25)-epoxy-23 xi -methoxyl-3 beta ,6 alpha ,12 beta -trihydroxydamm-ar-20(22)(E)-ene 2, respectively.
