A new furost-20(22)-ene oligoglycoside named as aspacochioside C was isolated from the roots of Asparagus cochinchinensis (Lour.) Merr.. Its structure was elucidated to be 26-O-b- D-glucopyranosyl-(25S)-5b-furost-20(...A new furost-20(22)-ene oligoglycoside named as aspacochioside C was isolated from the roots of Asparagus cochinchinensis (Lour.) Merr.. Its structure was elucidated to be 26-O-b- D-glucopyranosyl-(25S)-5b-furost-20(22)-en-3b,26-diol-3-O-a-L-rhamnopyranosyl-(14)-b-D- glucopyranoside on the basis of spectroscopic techniques including 1D and 2D NMR experiments.展开更多
A modified method of preparing 20(S)-ginsenoside Rh2(G-Rh2) and the inhibitory effect of 20(S)-ginsenoside Rh2 on Hep-A-22 cells were investigated. The total saponins and strong alkali were dissolved in glycerol...A modified method of preparing 20(S)-ginsenoside Rh2(G-Rh2) and the inhibitory effect of 20(S)-ginsenoside Rh2 on Hep-A-22 cells were investigated. The total saponins and strong alkali were dissolved in glycerol at the atmospheric pressure, and the degradation was performed at a high temperature. After G-Rh2 had been isolated and purified, MTT(methyl thiazolyl tetrazolium) assay was applied to evaluating the effect of 20(S)-ginsenoside Rh2 on the cells viability and morphological changes were observed. It was shown that 20(S)-ginsenoside Rh2 can reduce Hep-A-22 cells viability in dose-dependent manner and the cells took on cell shrinkage, membrane blebbing, chromosomal condensations, especially under the higher concentrations of it. In conclusion, 20(S)-ginsenoside Rh2 can be prepared effectively that not only decreases viability but also induces the apoptosis of Hep-A-22 cells.展开更多
O-a-D-Glucopyranosyl-furost-5(6),20(22)-dien-3a,26-diol 1, a new furostanol saponin, was isolated from the water-extract of Dioscorea nipponica Mak., the raw material of a traditional Chinese herbal medicine Wei Ao Xi...O-a-D-Glucopyranosyl-furost-5(6),20(22)-dien-3a,26-diol 1, a new furostanol saponin, was isolated from the water-extract of Dioscorea nipponica Mak., the raw material of a traditional Chinese herbal medicine Wei Ao Xin. The structure of 1 was determined on the basis of its spectral data especially by NMR spectroscopy. The result provides the first example of naturally occurring furostanol saponins with a single saccharide chain at the C-26 position.展开更多
文摘A new furost-20(22)-ene oligoglycoside named as aspacochioside C was isolated from the roots of Asparagus cochinchinensis (Lour.) Merr.. Its structure was elucidated to be 26-O-b- D-glucopyranosyl-(25S)-5b-furost-20(22)-en-3b,26-diol-3-O-a-L-rhamnopyranosyl-(14)-b-D- glucopyranoside on the basis of spectroscopic techniques including 1D and 2D NMR experiments.
基金Supported by the National Natural Science Foundation of China(No.30672654)
文摘A modified method of preparing 20(S)-ginsenoside Rh2(G-Rh2) and the inhibitory effect of 20(S)-ginsenoside Rh2 on Hep-A-22 cells were investigated. The total saponins and strong alkali were dissolved in glycerol at the atmospheric pressure, and the degradation was performed at a high temperature. After G-Rh2 had been isolated and purified, MTT(methyl thiazolyl tetrazolium) assay was applied to evaluating the effect of 20(S)-ginsenoside Rh2 on the cells viability and morphological changes were observed. It was shown that 20(S)-ginsenoside Rh2 can reduce Hep-A-22 cells viability in dose-dependent manner and the cells took on cell shrinkage, membrane blebbing, chromosomal condensations, especially under the higher concentrations of it. In conclusion, 20(S)-ginsenoside Rh2 can be prepared effectively that not only decreases viability but also induces the apoptosis of Hep-A-22 cells.
文摘A new phytoecdysteroid was isolated from the root of Rhaponticum uniflorum. Its structure has been shown to be 2, 3, 20, 22-diacetonide ajugasterone.
文摘O-a-D-Glucopyranosyl-furost-5(6),20(22)-dien-3a,26-diol 1, a new furostanol saponin, was isolated from the water-extract of Dioscorea nipponica Mak., the raw material of a traditional Chinese herbal medicine Wei Ao Xin. The structure of 1 was determined on the basis of its spectral data especially by NMR spectroscopy. The result provides the first example of naturally occurring furostanol saponins with a single saccharide chain at the C-26 position.