目的:分析长链非编码RNA(long non-coding RNA,lnc RNA)BC200在非小细胞肺癌(non-small cell lung cancer,NSCLC)组织中的表达情况,并探讨其临床意义及其与上皮-间质转化(epithelial-mesenchymal transition,EMT)相关蛋白E-cadherin、N-...目的:分析长链非编码RNA(long non-coding RNA,lnc RNA)BC200在非小细胞肺癌(non-small cell lung cancer,NSCLC)组织中的表达情况,并探讨其临床意义及其与上皮-间质转化(epithelial-mesenchymal transition,EMT)相关蛋白E-cadherin、N-cadherin及Snail之间的关系。方法:采用实时荧光定量PCR(QPCR)法检测2014年1月至2018年6月天津市胸科医院60例癌组织及60例对应的正常肺组织中BC200表达情况,40例癌组织及40例正常肺组织中E-cadherin、N-cadherin及Snail m RNA表达水平,统计分析NSCLC中BC200与E-cadherin、N-cadherin及Snail m RNA表达水平以及两者的相关性,分析BC200与临床病理特征(性别、年龄、TNM分期、肿瘤大小、淋巴结转移及病理类型水平)的关系,应用受试者工作特征(receiver operating characteristic curve,ROC)曲线鉴别诊断NSCLC的效能;采用免疫组织化学法检测E-cadherin、N-cadherin及Snail蛋白在40例NSCLC组织及20例正常肺组织中表达情况,分析其与BC200表达情况的关系。结果:1)肺癌组织中的BC200、N-cadherin及Snail m RNA的表达水平显著高于正常肺组织中的表达水平(P<0.05),E-cadherin m RNA的表达量低于正常肺组织(P<0.05);2)E-cadherin、N-cadherin及Snail在NSCLC组织中阳性表达率分别为40.0%(16/40)、57.5%(23/40)、57.5%(23/40),在正常肺组织中的阳性表达率为95%(19/20)、5%(1/20)、10%(2/20),表达差异具有统计学意义(P<0.05);3)肺癌组织中BC200的高表达与淋巴结转移、临床分期、E-cadherin、N-cadherin及Snail阳性率相关(P<0.05),BC200的表达与E-cadherin m RNA的表达呈负相关(r=-0.31,P<0.05),与Snail、N-cadherin m RNA的表达呈正相关(r=0.305、r=0.257,P<0.05);4)ROC曲线分析表明,组织lnc RNA表达水平对NSCLC的诊断敏感度和特异度均较满意。结论:BC200在NSCLC组织中高表达,且与淋巴结转移、临床分期、E-cadherin、N-cadherin及Snail阳性率相关,BC200的表达与E-cadherin m RNA的表达呈负相关,与Snail、N-cadherin m RNA的表达呈正相关,提示BC200可能通过上皮间质转化调节肺癌细胞侵袭和迁移能力。在NSCLC诊断上,lncRNA BC200水平有较高的价值,可考虑作为NSCLC诊断的潜在标记物。展开更多
X脆性综合征(fragile X syndrome,FXS)是由X脆性智力低下1(FMR1)基因5'端非翻译区CGG重复序列的异常扩增,导致X脆性智力低下蛋白(FMRP)缺失引起的。非编码RNA是除编码蛋白质的mRNAs以外的其他所有RNA分子,已被发现在中枢神经系统中...X脆性综合征(fragile X syndrome,FXS)是由X脆性智力低下1(FMR1)基因5'端非翻译区CGG重复序列的异常扩增,导致X脆性智力低下蛋白(FMRP)缺失引起的。非编码RNA是除编码蛋白质的mRNAs以外的其他所有RNA分子,已被发现在中枢神经系统中具有重要的作用,如微RNA与BC1/BC200RNA参与了X脆性智力低下蛋白的翻译抑制。认识非编码RNA与X脆性综合征的关系不但能加深对X脆性综合征的分子机制的理解,而且有助于揭示学习与记忆的奥秘。展开更多
Nearly 200000 animal skeletons are unearthed in the Unit T0202 from Zhongba Site of Zhongxian County.According to the analysis of 129165 specimens,these skeletons may be classified into 5 kinds, namely,Mammalia,Osteic...Nearly 200000 animal skeletons are unearthed in the Unit T0202 from Zhongba Site of Zhongxian County.According to the analysis of 129165 specimens,these skeletons may be classified into 5 kinds, namely,Mammalia,Osteichthys,Aves,Amphibian and Reptilia,which belong to 13 orders,28 families and 42 genera.In this paper,based on archeological dating and AMS 14 C data,through statistically analyzing the unearthed skeletons and studying the change of the smallest individual numbers,these research results detected the following:1)In almost all the time of 2370―200 BC,in Zhongba region, some animals distributed widely,such as Muntiacus sp,Elaphodus cephalophus and Muntiacus sp. inhabiting in glade and grassland,Scrofa sp.and Canis familiaris raised by the ancient people,rodentia rabbit and Rattus rattus,which suggested that a fairly good ecological environment of forest and grassland was preserved at that period and the predecessors began to raise domestic animals from 1750 to 1000 BC,which has lasted until today.2)Rhinoceros only lived during 2000―1750 BC,1000― 700 BC and 700―500 BC,which indicates that the ecological environment of grassland and wetland might be better in these phases.3)Macaca sp.and Ursus arctos appeared only after 1750―1000 BC, which may show that the forest condition is better for animals to live during 1750―200 BC.4)The smallest individual numbers of Bublus sp.,bos sp.and otters emerged during about 2370―1750 BC, which perhaps infers that water area during the early period was wider than that of the late period.5) Since skeletons of rhinoceros are discovered in strata of Zhongba Site during 2000―1750 BC and 1000―500 BC,according to the climate and ecology environment which rhinoceros live in now,the average annual temperature and precipitation during 2000―1750 BC and 1000―500 BC are supposed to be probably higher than that of today.Although Zhu Kezhen considered that the first low temperature period in the past 5000 years of China was between 1100 BC and 850 BC,massive pollen of Morus, Ulmus,Fagus,Quercus,Castanea,etc.were found in Dajiuhu peat at that time,which should indicate that the climate was still moderate for living things to live at least in Dajiuhu basin and Zhongba Site.6) Because skeletons of Gervus albirostris were unearthed during 310―200 BC at Zhongba Site,based onthe climate and the ecological environment those animals live in now,the average annual temperature and the average annual precipitation in Zhongba area between 310 BC and 200 BC should be lower than those of today,which is confirmed by the TOC research of Zhongba Site strata.展开更多
文摘目的:分析长链非编码RNA(long non-coding RNA,lnc RNA)BC200在非小细胞肺癌(non-small cell lung cancer,NSCLC)组织中的表达情况,并探讨其临床意义及其与上皮-间质转化(epithelial-mesenchymal transition,EMT)相关蛋白E-cadherin、N-cadherin及Snail之间的关系。方法:采用实时荧光定量PCR(QPCR)法检测2014年1月至2018年6月天津市胸科医院60例癌组织及60例对应的正常肺组织中BC200表达情况,40例癌组织及40例正常肺组织中E-cadherin、N-cadherin及Snail m RNA表达水平,统计分析NSCLC中BC200与E-cadherin、N-cadherin及Snail m RNA表达水平以及两者的相关性,分析BC200与临床病理特征(性别、年龄、TNM分期、肿瘤大小、淋巴结转移及病理类型水平)的关系,应用受试者工作特征(receiver operating characteristic curve,ROC)曲线鉴别诊断NSCLC的效能;采用免疫组织化学法检测E-cadherin、N-cadherin及Snail蛋白在40例NSCLC组织及20例正常肺组织中表达情况,分析其与BC200表达情况的关系。结果:1)肺癌组织中的BC200、N-cadherin及Snail m RNA的表达水平显著高于正常肺组织中的表达水平(P<0.05),E-cadherin m RNA的表达量低于正常肺组织(P<0.05);2)E-cadherin、N-cadherin及Snail在NSCLC组织中阳性表达率分别为40.0%(16/40)、57.5%(23/40)、57.5%(23/40),在正常肺组织中的阳性表达率为95%(19/20)、5%(1/20)、10%(2/20),表达差异具有统计学意义(P<0.05);3)肺癌组织中BC200的高表达与淋巴结转移、临床分期、E-cadherin、N-cadherin及Snail阳性率相关(P<0.05),BC200的表达与E-cadherin m RNA的表达呈负相关(r=-0.31,P<0.05),与Snail、N-cadherin m RNA的表达呈正相关(r=0.305、r=0.257,P<0.05);4)ROC曲线分析表明,组织lnc RNA表达水平对NSCLC的诊断敏感度和特异度均较满意。结论:BC200在NSCLC组织中高表达,且与淋巴结转移、临床分期、E-cadherin、N-cadherin及Snail阳性率相关,BC200的表达与E-cadherin m RNA的表达呈负相关,与Snail、N-cadherin m RNA的表达呈正相关,提示BC200可能通过上皮间质转化调节肺癌细胞侵袭和迁移能力。在NSCLC诊断上,lncRNA BC200水平有较高的价值,可考虑作为NSCLC诊断的潜在标记物。
文摘X脆性综合征(fragile X syndrome,FXS)是由X脆性智力低下1(FMR1)基因5'端非翻译区CGG重复序列的异常扩增,导致X脆性智力低下蛋白(FMRP)缺失引起的。非编码RNA是除编码蛋白质的mRNAs以外的其他所有RNA分子,已被发现在中枢神经系统中具有重要的作用,如微RNA与BC1/BC200RNA参与了X脆性智力低下蛋白的翻译抑制。认识非编码RNA与X脆性综合征的关系不但能加深对X脆性综合征的分子机制的理解,而且有助于揭示学习与记忆的奥秘。
基金Supported by the National Natural Science Foundation of China(Grant No.90411015)the University Doctoral Foundation(Grant No.20050284011)+2 种基金the Foundation of Important Basic Research at Nanjing University(Grant No.0209005206)Open Foundation of the State Key Laboratory of Loess and Quaternary Geology from the Institute of Earth Environment,CAS(Grant No.SKLLQG0503)the Physical Geography of"985"Items and the Test Foundation of Modern Analyses Center of Nanjing University(Grant No.0209001309)
文摘Nearly 200000 animal skeletons are unearthed in the Unit T0202 from Zhongba Site of Zhongxian County.According to the analysis of 129165 specimens,these skeletons may be classified into 5 kinds, namely,Mammalia,Osteichthys,Aves,Amphibian and Reptilia,which belong to 13 orders,28 families and 42 genera.In this paper,based on archeological dating and AMS 14 C data,through statistically analyzing the unearthed skeletons and studying the change of the smallest individual numbers,these research results detected the following:1)In almost all the time of 2370―200 BC,in Zhongba region, some animals distributed widely,such as Muntiacus sp,Elaphodus cephalophus and Muntiacus sp. inhabiting in glade and grassland,Scrofa sp.and Canis familiaris raised by the ancient people,rodentia rabbit and Rattus rattus,which suggested that a fairly good ecological environment of forest and grassland was preserved at that period and the predecessors began to raise domestic animals from 1750 to 1000 BC,which has lasted until today.2)Rhinoceros only lived during 2000―1750 BC,1000― 700 BC and 700―500 BC,which indicates that the ecological environment of grassland and wetland might be better in these phases.3)Macaca sp.and Ursus arctos appeared only after 1750―1000 BC, which may show that the forest condition is better for animals to live during 1750―200 BC.4)The smallest individual numbers of Bublus sp.,bos sp.and otters emerged during about 2370―1750 BC, which perhaps infers that water area during the early period was wider than that of the late period.5) Since skeletons of rhinoceros are discovered in strata of Zhongba Site during 2000―1750 BC and 1000―500 BC,according to the climate and ecology environment which rhinoceros live in now,the average annual temperature and precipitation during 2000―1750 BC and 1000―500 BC are supposed to be probably higher than that of today.Although Zhu Kezhen considered that the first low temperature period in the past 5000 years of China was between 1100 BC and 850 BC,massive pollen of Morus, Ulmus,Fagus,Quercus,Castanea,etc.were found in Dajiuhu peat at that time,which should indicate that the climate was still moderate for living things to live at least in Dajiuhu basin and Zhongba Site.6) Because skeletons of Gervus albirostris were unearthed during 310―200 BC at Zhongba Site,based onthe climate and the ecological environment those animals live in now,the average annual temperature and the average annual precipitation in Zhongba area between 310 BC and 200 BC should be lower than those of today,which is confirmed by the TOC research of Zhongba Site strata.
