A new triterpenoid. 3 beta. 4 beta. 23-trihydroxy-24, 30-dinorolean-12. 20(29)-dien-28-oic acid. together with five known compounds. 2 alpha. 3 beta. 23-trihydroxy-12-oleanen-28-oic acid-beta -D-glucose glucopyranosyl...A new triterpenoid. 3 beta. 4 beta. 23-trihydroxy-24, 30-dinorolean-12. 20(29)-dien-28-oic acid. together with five known compounds. 2 alpha. 3 beta. 23-trihydroxy-12-oleanen-28-oic acid-beta -D-glucose glucopyranosyl ester. palbinone. 2-hydroxy-benzoic acid, vanillic acid. syringic acid. were isolated from the roots of Paeonia delavayi Franch. Their structures were characterized by spectral analysis.展开更多
AIM:To investigate the chemical constituents of the stems,leaves and roots of Euphorbia hirta,and to test for the cytotoxic and antimicrobial potentials of the major constituents of the plant.METHODS: The compounds we...AIM:To investigate the chemical constituents of the stems,leaves and roots of Euphorbia hirta,and to test for the cytotoxic and antimicrobial potentials of the major constituents of the plant.METHODS: The compounds were isolated by silica gel chromatography and their structures were elucidated by NMR spectroscopy.The cytotoxicity tests were conducted using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay,while the antimicrobial tests employed the agar well method.RESULTS: The air-dried stems of E.hirta afforded taraxerone 1,a mixture of 25-hydroperoxycycloart-23-en-3-ol(2a) and 24-hydroperoxycycloart-25-en-3-ol(2b)(sample 2) in a 2:1 ratio,and another mixture of cycloartenol(3a),lupeol(3b),-amyrin(3c) and-amyrin(3d)(sample 3) in a 0.5:4:1:1 ratio.The air-dried leaves of E.hirta yielded sample 2 in a 3:2 ratio,sample 3 in a 2:3:1:1 ratio,phytol and phytyl fatty acid ester,while the roots afforded sample 2 in a 2:1 ratio,sample 3 in a 2:1:1:1 ratio,a mixture of cycloartenyl fatty acid ester 4a,lupeol fatty acid ester 4b,-amyrin fatty acid ester 4c and-amyrin fatty acid ester 4d(sample 4) in a 3:2:1:1 ratio,linoleic acid,-sitosterol and squalene.Compound 1 from the stems,sample 2 from the leaves,and sample 3 from the stems were assessed for cytotoxicity against a human cancer cell line,colon carcinoma(HCT 116).Sample 2 showed good activity with an IC50 value of 4.8 g·mL 1,while 1 and sample 3 were inactive against HCT 116.Sample 2 was further tested for cytotoxicity against non-small cell lung adenocarcinoma(A549).It showed good activity against this cell line with an IC50 value of 4.5 g·mL 1.Antimicrobial assays were conducted on 1 and sample 2.Results of the study indicated that 1 was active against the bacteria: Pseudomonas aeruginosa and Staphylococcus aureus,but was inactive against Escherichia coli and Bacillus subtilis.Sample 2 was active against the bacteria: Pseudomonas aeruginosa,Staphylococcus aureus and Escherichia coli and fungi: Candida albicans and Trichophyton mentagrophytes.It was inactive against Bacillus subtilis and Aspergillus niger.CONCLUSIONS: The triterpenes: 2a,2b,3a,3b,3c and 3d were obtained from the stems,roots and leaves of E.hirta.Taraxerol(1) was only isolated from the stems,the leaves yielded phytol and phytyl fatty acid esters,while the roots afforded 4a-4d,linoleic acid,-sitosterol,and squalene.Triterpene 1 and sample 2 were found to exhibit antimicrobial activities.Thus,these compounds are some of the active principles of E.hirta which is used in wound healing and the treatment of boils.The cytotoxic properties of sample 2 imply that triterpenes 2a and 2b contribute to the anticancer activity of E.hirta.展开更多
Objective To study the chemical constituents in the acid-hydrolyzed ethanol extract from Momordica charantia.Methods The ethanol extract from M.charantia was hydrolyzed by 36%HCl and the hydrolysate was isolated by si...Objective To study the chemical constituents in the acid-hydrolyzed ethanol extract from Momordica charantia.Methods The ethanol extract from M.charantia was hydrolyzed by 36%HCl and the hydrolysate was isolated by silica gel column chromatography and preparative HPLC.The structures of the isolated compounds were identified by spectral analyses,physical constants,and chemical evidences.Results Two cucurbitane triterpenoids were isolated and identified as 5β,19-epoxy-cucurbita-6,22E,24-trien-3β-ol(1)and cucurbita-6,22(E),24-trien-3β-ol-19,5β-olide(2).Conclusion Compound 1 is a new compound.展开更多
文摘A new triterpenoid. 3 beta. 4 beta. 23-trihydroxy-24, 30-dinorolean-12. 20(29)-dien-28-oic acid. together with five known compounds. 2 alpha. 3 beta. 23-trihydroxy-12-oleanen-28-oic acid-beta -D-glucose glucopyranosyl ester. palbinone. 2-hydroxy-benzoic acid, vanillic acid. syringic acid. were isolated from the roots of Paeonia delavayi Franch. Their structures were characterized by spectral analysis.
基金supported by the grant from the De La Salle University Science Foundation
文摘AIM:To investigate the chemical constituents of the stems,leaves and roots of Euphorbia hirta,and to test for the cytotoxic and antimicrobial potentials of the major constituents of the plant.METHODS: The compounds were isolated by silica gel chromatography and their structures were elucidated by NMR spectroscopy.The cytotoxicity tests were conducted using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay,while the antimicrobial tests employed the agar well method.RESULTS: The air-dried stems of E.hirta afforded taraxerone 1,a mixture of 25-hydroperoxycycloart-23-en-3-ol(2a) and 24-hydroperoxycycloart-25-en-3-ol(2b)(sample 2) in a 2:1 ratio,and another mixture of cycloartenol(3a),lupeol(3b),-amyrin(3c) and-amyrin(3d)(sample 3) in a 0.5:4:1:1 ratio.The air-dried leaves of E.hirta yielded sample 2 in a 3:2 ratio,sample 3 in a 2:3:1:1 ratio,phytol and phytyl fatty acid ester,while the roots afforded sample 2 in a 2:1 ratio,sample 3 in a 2:1:1:1 ratio,a mixture of cycloartenyl fatty acid ester 4a,lupeol fatty acid ester 4b,-amyrin fatty acid ester 4c and-amyrin fatty acid ester 4d(sample 4) in a 3:2:1:1 ratio,linoleic acid,-sitosterol and squalene.Compound 1 from the stems,sample 2 from the leaves,and sample 3 from the stems were assessed for cytotoxicity against a human cancer cell line,colon carcinoma(HCT 116).Sample 2 showed good activity with an IC50 value of 4.8 g·mL 1,while 1 and sample 3 were inactive against HCT 116.Sample 2 was further tested for cytotoxicity against non-small cell lung adenocarcinoma(A549).It showed good activity against this cell line with an IC50 value of 4.5 g·mL 1.Antimicrobial assays were conducted on 1 and sample 2.Results of the study indicated that 1 was active against the bacteria: Pseudomonas aeruginosa and Staphylococcus aureus,but was inactive against Escherichia coli and Bacillus subtilis.Sample 2 was active against the bacteria: Pseudomonas aeruginosa,Staphylococcus aureus and Escherichia coli and fungi: Candida albicans and Trichophyton mentagrophytes.It was inactive against Bacillus subtilis and Aspergillus niger.CONCLUSIONS: The triterpenes: 2a,2b,3a,3b,3c and 3d were obtained from the stems,roots and leaves of E.hirta.Taraxerol(1) was only isolated from the stems,the leaves yielded phytol and phytyl fatty acid esters,while the roots afforded 4a-4d,linoleic acid,-sitosterol,and squalene.Triterpene 1 and sample 2 were found to exhibit antimicrobial activities.Thus,these compounds are some of the active principles of E.hirta which is used in wound healing and the treatment of boils.The cytotoxic properties of sample 2 imply that triterpenes 2a and 2b contribute to the anticancer activity of E.hirta.
基金National New Drug Incubation(Benxi)Base Construction of Liaoning Province(2010ZX09401-304-105B)the Doctoral InitiatingFoundation Project of Liaoning Province(20101108)
文摘Objective To study the chemical constituents in the acid-hydrolyzed ethanol extract from Momordica charantia.Methods The ethanol extract from M.charantia was hydrolyzed by 36%HCl and the hydrolysate was isolated by silica gel column chromatography and preparative HPLC.The structures of the isolated compounds were identified by spectral analyses,physical constants,and chemical evidences.Results Two cucurbitane triterpenoids were isolated and identified as 5β,19-epoxy-cucurbita-6,22E,24-trien-3β-ol(1)and cucurbita-6,22(E),24-trien-3β-ol-19,5β-olide(2).Conclusion Compound 1 is a new compound.
