This article aims to study the effects of exogenous 24-epibrassinolide （EBR） on the changes in ROS, activities of antioxidative enzymes and antioxidants in cucumber （Cucumis sativus L.） seedling roots under hypoxi...This article aims to study the effects of exogenous 24-epibrassinolide （EBR） on the changes in ROS, activities of antioxidative enzymes and antioxidants in cucumber （Cucumis sativus L.） seedling roots under hypoxia stress. Seedlings of a hypoxiaresistant cultivar, Lühachun 4, and a hypoxia-sensitive cultivar, Zhongnong 8, were hydroponically grown for 8 d in normoxic or hypoxic nutrient solutions that were added or not added with 10^-3 mg L^-1 EBR. Under hypoxia stress, the ROS levels and the lipid peroxidation were significantly increased in the roots upon exposure to hypoxia stress, which were inhibited by EBR application. The EBR treatment significantly increased the seedlings growth and SOD, APX, GR activities, and contents of AsA and GSH under hypoxia stress. From the results obtained in this study, it can be concluded that oxidative damage on seedling roots by hypoxia stress can be considerably alleviated and the tolerance of plants was elevated.展开更多
High grain-Cd-accumulating rice variety Yongyou 9 was planted in Cd-contaminated farmland in Taizhou City, Zhejiang Province, China to study the effects of 5-aminolevulinic acid(ALA) and24-epibrassinolide(EBR) on Cd a...High grain-Cd-accumulating rice variety Yongyou 9 was planted in Cd-contaminated farmland in Taizhou City, Zhejiang Province, China to study the effects of 5-aminolevulinic acid(ALA) and24-epibrassinolide(EBR) on Cd accumulation in brown rice. Results showed that the exogenous ALA and EBR had no significant effects on agronomic traits, soil pH and total Cd content in soil, but had some effects on the available Cd content in soil, and significantly influenced the Cd accumulation in the different parts of rice. Results also showed that 100 mg/L exogenous ALA significantly reduced the Cd accumulation in brown rice to blow the food safety standard(0.2 mg/kg), and also significantly reduced the Cd contents in the roots and culm of rice. However, 200 mg/L exogenous ALA treatment increased the Cd content in brown rice remarkably. In addition, 0.15 mg/L EBR treatment increased Cd accumulation in roots, culm, leaves and brown rice notably, whereas 0.30 mg/L exogenous EBR treatment reduced the Cd accumulation in brown rice properly, but it was not significant. Therefore,proper concentration of ALA can effectively reduce the Cd accumulation in brown rice, which can be used as an effective technical method for the safe production of rice in Cd polluted farmland.展开更多
In this work we compared the effect of the growth regulator content of the culture medium on the growth of in vitro shoot tips of five yam accessions belonging to four yam species (one Dioscorea alata, one D. rotundat...In this work we compared the effect of the growth regulator content of the culture medium on the growth of in vitro shoot tips of five yam accessions belonging to four yam species (one Dioscorea alata, one D. rotundata, one D. cayenensis and two D. trifida). Medium S contained 0.6 μM benzyl adenine, 1.07 μM naphthalene acetic acid and 0.23 μM gibberellic acid while medium EBR contained 0.23 μM gibberellic acid and 0.1 μM 24-epibrassinolide. After 2 months of culture, oxidation level was significantly reduced on medium EBR compared to medium S for four of the five accessions tested. By contrast, medium EBR did not have any positive effect on shoot length since length of shoots produced after 2 months of culture on medium S and EBR were similar, except with accession 3-45T, for which shoot length was shorter on medium S compared to medium EBR. These results underline the potential of 24-epibrassinolide to reduce oxidation phenomena during in vitro culture and call for its utilization for regeneration of cryopreserved yam shoot tips, which is often impeded by oxidation phenomena.展开更多
The effect of 24-epibrassinolide on growth of pigeon pea [Cajanus cajan (L.) Millsp.] under aluminium toxicity was studied. 24-EBL reduced the impact of Al stress on plant growth. Particularly 24-EBL reduced the inhib...The effect of 24-epibrassinolide on growth of pigeon pea [Cajanus cajan (L.) Millsp.] under aluminium toxicity was studied. 24-EBL reduced the impact of Al stress on plant growth. Particularly 24-EBL reduced the inhibitory impact of aluminium toxicity on root growth which was further manifested in overall improvement of vegetative growth. Application of 24-epibrassinolide removed the inhibitory influence of Al nodulation. The growth stimulation in Cajanus plants by 24-EBL under Al stress was associated with elevated levels of chlorophylls, nucleic acids and soluble proteins. 24-Epibrassinolide application enhanced proline content in Al<sup>3+</sup> stressed Cajanus plants. Further, the supplementation of 24-epibrassinolide to Al stress treatments increased the activities of antioxidative enzymes viz., catalase [EC 1.11.1.6];peroxidase [EC 1.11.1.7];superoxide dismutase [EC 1.15.1.1] and ascorbate peroxidase [EC 1.11.1.11]. Lipid peroxidation induced by Al was found reduced with the supplementation of 24-epibrassinolide. The present studies demonstrated the ameliorating capability of 24-epibrassinolide on the Al induced inhibition of plant growth of C. cajan.展开更多
安全生产事故往往由多组织交互、多因素耦合造成,事故原因涉及多个组织。为预防和遏制多组织生产安全事故的发生,基于系统理论事故建模与过程模型(Systems-Theory Accident Modeling and Process,STAMP)、24Model,构建一种用于多组织事...安全生产事故往往由多组织交互、多因素耦合造成,事故原因涉及多个组织。为预防和遏制多组织生产安全事故的发生,基于系统理论事故建模与过程模型(Systems-Theory Accident Modeling and Process,STAMP)、24Model,构建一种用于多组织事故分析的方法,并以青岛石油爆炸事故为例进行事故原因分析。结果显示:STAMP-24Model可以分组织,分层次且有效、全面、详细地分析涉及多个组织的事故原因,探究多组织之间的交互关系;对事故进行动态演化分析,可得到各组织不安全动作耦合关系与形成的事故失效链及管控失效路径,进而为预防多组织事故提供思路和参考。展开更多
文摘This article aims to study the effects of exogenous 24-epibrassinolide （EBR） on the changes in ROS, activities of antioxidative enzymes and antioxidants in cucumber （Cucumis sativus L.） seedling roots under hypoxia stress. Seedlings of a hypoxiaresistant cultivar, Lühachun 4, and a hypoxia-sensitive cultivar, Zhongnong 8, were hydroponically grown for 8 d in normoxic or hypoxic nutrient solutions that were added or not added with 10^-3 mg L^-1 EBR. Under hypoxia stress, the ROS levels and the lipid peroxidation were significantly increased in the roots upon exposure to hypoxia stress, which were inhibited by EBR application. The EBR treatment significantly increased the seedlings growth and SOD, APX, GR activities, and contents of AsA and GSH under hypoxia stress. From the results obtained in this study, it can be concluded that oxidative damage on seedling roots by hypoxia stress can be considerably alleviated and the tolerance of plants was elevated.
基金supported by the Natural Science Foundation of Zhejiang Province,China(Grant No.LY17C020005)the Key Research and Development Project of Zhejiang Province,China(Grant No.2015C03020-4)+2 种基金the National Nature Science Foundation of China(Grant No.31401356)Jinhua Science and Technology Project(Grant No.2015-2-012)the National Training Program for College Students to Innovate and Start Enterprise(Grant No.201710356013)
文摘High grain-Cd-accumulating rice variety Yongyou 9 was planted in Cd-contaminated farmland in Taizhou City, Zhejiang Province, China to study the effects of 5-aminolevulinic acid(ALA) and24-epibrassinolide(EBR) on Cd accumulation in brown rice. Results showed that the exogenous ALA and EBR had no significant effects on agronomic traits, soil pH and total Cd content in soil, but had some effects on the available Cd content in soil, and significantly influenced the Cd accumulation in the different parts of rice. Results also showed that 100 mg/L exogenous ALA significantly reduced the Cd accumulation in brown rice to blow the food safety standard(0.2 mg/kg), and also significantly reduced the Cd contents in the roots and culm of rice. However, 200 mg/L exogenous ALA treatment increased the Cd content in brown rice remarkably. In addition, 0.15 mg/L EBR treatment increased Cd accumulation in roots, culm, leaves and brown rice notably, whereas 0.30 mg/L exogenous EBR treatment reduced the Cd accumulation in brown rice properly, but it was not significant. Therefore,proper concentration of ALA can effectively reduce the Cd accumulation in brown rice, which can be used as an effective technical method for the safe production of rice in Cd polluted farmland.
文摘In this work we compared the effect of the growth regulator content of the culture medium on the growth of in vitro shoot tips of five yam accessions belonging to four yam species (one Dioscorea alata, one D. rotundata, one D. cayenensis and two D. trifida). Medium S contained 0.6 μM benzyl adenine, 1.07 μM naphthalene acetic acid and 0.23 μM gibberellic acid while medium EBR contained 0.23 μM gibberellic acid and 0.1 μM 24-epibrassinolide. After 2 months of culture, oxidation level was significantly reduced on medium EBR compared to medium S for four of the five accessions tested. By contrast, medium EBR did not have any positive effect on shoot length since length of shoots produced after 2 months of culture on medium S and EBR were similar, except with accession 3-45T, for which shoot length was shorter on medium S compared to medium EBR. These results underline the potential of 24-epibrassinolide to reduce oxidation phenomena during in vitro culture and call for its utilization for regeneration of cryopreserved yam shoot tips, which is often impeded by oxidation phenomena.
文摘The effect of 24-epibrassinolide on growth of pigeon pea [Cajanus cajan (L.) Millsp.] under aluminium toxicity was studied. 24-EBL reduced the impact of Al stress on plant growth. Particularly 24-EBL reduced the inhibitory impact of aluminium toxicity on root growth which was further manifested in overall improvement of vegetative growth. Application of 24-epibrassinolide removed the inhibitory influence of Al nodulation. The growth stimulation in Cajanus plants by 24-EBL under Al stress was associated with elevated levels of chlorophylls, nucleic acids and soluble proteins. 24-Epibrassinolide application enhanced proline content in Al<sup>3+</sup> stressed Cajanus plants. Further, the supplementation of 24-epibrassinolide to Al stress treatments increased the activities of antioxidative enzymes viz., catalase [EC 1.11.1.6];peroxidase [EC 1.11.1.7];superoxide dismutase [EC 1.15.1.1] and ascorbate peroxidase [EC 1.11.1.11]. Lipid peroxidation induced by Al was found reduced with the supplementation of 24-epibrassinolide. The present studies demonstrated the ameliorating capability of 24-epibrassinolide on the Al induced inhibition of plant growth of C. cajan.
文摘安全生产事故往往由多组织交互、多因素耦合造成,事故原因涉及多个组织。为预防和遏制多组织生产安全事故的发生,基于系统理论事故建模与过程模型(Systems-Theory Accident Modeling and Process,STAMP)、24Model,构建一种用于多组织事故分析的方法,并以青岛石油爆炸事故为例进行事故原因分析。结果显示:STAMP-24Model可以分组织,分层次且有效、全面、详细地分析涉及多个组织的事故原因,探究多组织之间的交互关系;对事故进行动态演化分析,可得到各组织不安全动作耦合关系与形成的事故失效链及管控失效路径,进而为预防多组织事故提供思路和参考。
