A 24-membered ring macrolide compound,macrolactin A has potential applications in pharmaceuticals for its anti-infectious and antiviral activity.In this study,macrolactin A was produced by a marine bacterium,which was...A 24-membered ring macrolide compound,macrolactin A has potential applications in pharmaceuticals for its anti-infectious and antiviral activity.In this study,macrolactin A was produced by a marine bacterium,which was identified as Bacillus subtilis by 16S ribosomal RNA(rRNA) sequence analysis.Electrospray ionization mass spectrometry(ESI/MS) and nuclear magnetic resonance(NMR) spectroscopy analyses were used to characterize this compound.To improve the production,response surface methodology(RSM) involving Box-Behnken design(BBD) was employed.Faeces bombycis,the main by-product in sericulture,was used as a nitrogen source in fermentation.The interactions between three significant factors,F.bombycis,soluble starch,and(NH4)2SO4 were investigated.A quadratic model was constructed to fit the production and the factors.Optimum medium composition was obtained by analysis of the model.When cultivated in the optimum medium,the production of macrolactin A was increased to 851 mg/L,2.7 times as compared to the original.This study is also useful to find another way in utilizing F.bombycis.展开更多
One new indoloditerpene,6,7-dehydropaxilline（1）,one new indole-diketopiperazine,spirotryprostatin F（2）,and one new 13-membered macrolide,N-demethylmelearoride A（3）,as well as 8 known alkaloids（4-11）,were isola...One new indoloditerpene,6,7-dehydropaxilline（1）,one new indole-diketopiperazine,spirotryprostatin F（2）,and one new 13-membered macrolide,N-demethylmelearoride A（3）,as well as 8 known alkaloids（4-11）,were isolated from the solid cultures of the endophytic fungus,Penicillium brefeidianum.The structures and absolute configurations of compounds 1-3 were elucidated by comprehensive spectroscopic analysis and the circular dichroism（CD） exciton chirality method.All the metablites were evaluated for their cytotoxic activites against three human tumor cell lines.Compound 2 exhibited cytotoxicities against HepG2 and MDA-MB-231 cells with IC（50） values of 14.1 μmol/L and 35.5μmol/L,respectively.Compound 3 showed moderate activity against HepG2 cells with IC（50） values of 36.6 μmol/L.展开更多
基金Project supported by the Science and Technology Department of Zhejiang Province,China(No.2009C33019)the IndustryUniversity-Research Institution Alliance for Microbial Medicine Technology Innovation and New Drug Development of China (No.2010ZX090401-403)the National Science and Technology Major Project of New Drug of China(Nos.2011ZX09201-101 and 2012ZX09103101-075)
文摘A 24-membered ring macrolide compound,macrolactin A has potential applications in pharmaceuticals for its anti-infectious and antiviral activity.In this study,macrolactin A was produced by a marine bacterium,which was identified as Bacillus subtilis by 16S ribosomal RNA(rRNA) sequence analysis.Electrospray ionization mass spectrometry(ESI/MS) and nuclear magnetic resonance(NMR) spectroscopy analyses were used to characterize this compound.To improve the production,response surface methodology(RSM) involving Box-Behnken design(BBD) was employed.Faeces bombycis,the main by-product in sericulture,was used as a nitrogen source in fermentation.The interactions between three significant factors,F.bombycis,soluble starch,and(NH4)2SO4 were investigated.A quadratic model was constructed to fit the production and the factors.Optimum medium composition was obtained by analysis of the model.When cultivated in the optimum medium,the production of macrolactin A was increased to 851 mg/L,2.7 times as compared to the original.This study is also useful to find another way in utilizing F.bombycis.
基金funded by the National Natural Science Foundation of China(No.81503218)the Program for Changjiang Scholars and Innovative Research Team in University(No.IRT_15R63)+1 种基金the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)the Fundamental Research Funds for the Central Universities(No.2016ZZD010)
文摘One new indoloditerpene,6,7-dehydropaxilline（1）,one new indole-diketopiperazine,spirotryprostatin F（2）,and one new 13-membered macrolide,N-demethylmelearoride A（3）,as well as 8 known alkaloids（4-11）,were isolated from the solid cultures of the endophytic fungus,Penicillium brefeidianum.The structures and absolute configurations of compounds 1-3 were elucidated by comprehensive spectroscopic analysis and the circular dichroism（CD） exciton chirality method.All the metablites were evaluated for their cytotoxic activites against three human tumor cell lines.Compound 2 exhibited cytotoxicities against HepG2 and MDA-MB-231 cells with IC（50） values of 14.1 μmol/L and 35.5μmol/L,respectively.Compound 3 showed moderate activity against HepG2 cells with IC（50） values of 36.6 μmol/L.
