AIM： To investigate the possible involvement of 25-hydroxyvitamin D3-1cx-hydroxylase [1α-25（OH）2D3] in butyrate-induced differentiation in human intestinal cell line Caco-2 cells. METHODS： Caco-2 cells were incub...AIM： To investigate the possible involvement of 25-hydroxyvitamin D3-1cx-hydroxylase [1α-25（OH）2D3] in butyrate-induced differentiation in human intestinal cell line Caco-2 cells. METHODS： Caco-2 cells were incubated either with 3 mmol/L butyrate and 1 umol/L 25（OH）2D3 or with 1 umol/L 1α-25（OH）2D3 for various time intervals ranging from 0 to 72 h. Additionally, cells were co-incubated with butyrate and either 25（OH）2D3 or 1α-25（OH）2D3. 1α-25（OH）2D3 mRNA was determined semi-quantitatively using the fluorescent dye PicoGreen. Immunoblotting was used for the detection of 1α-25（OH）2D3 protein. Finally, enzymatic activity was measured by ELISA. RESULTS： Both butyrate and 1α-25（OH）2D3 stimulated differentiation of Caco-2 cells after a 48 h incubation period, while 25（OH）2D3 had no impact on cell differentiation. Synergistic effects on differentiation were observed when cells were co-incubated with butyrate and vitamin D metabolite. Butyrate transiently upregulated 1α-25（OH）2D3 mRNA followed by a timely delayed protein upregulation. Coincidently, enzymatic activity was enhanced significantly. The induction of the enzyme allowed for comparable differentiating effects of both vitamin D metabolites. CONCLUSION： Our experimental data provide a further mechanism for the involvement of the vitamin D signaling pathway in colonic epithelial cell differentiation by butyrate. The enhancement of 1α-25（OH）2D3 followed by antiproliferative effects of the vitamin D prohormone in the Caco-2 cell line suggest that 25（OH）2D3 in combination with butyrate may offer a new therapeutic approach forthe treatment of colon cancer.展开更多
Objective To investigate the relationship between 25-hydroxy vitamin D3[25(OH)D3]level and sudomotor function(SF)in patients with type 2 diabetes mellitus(T2 DM).Methods A total of 1020 patients with T2 DM were collec...Objective To investigate the relationship between 25-hydroxy vitamin D3[25(OH)D3]level and sudomotor function(SF)in patients with type 2 diabetes mellitus(T2 DM).Methods A total of 1020 patients with T2 DM were collected in this study from Endocrinology Department in Jinling College of Clinical Medicine,Nanjing Medical University during January 2017 to December 2019.展开更多
The novel 19 nor l α ,25 dihydroxy vitamin D 3 analogues possessing an ethyl at the 2 position(4 and 5), were synthesized by coupling 25 hydroxy Windaus Grundmann ketone derivative 20 with A ring syntho...The novel 19 nor l α ,25 dihydroxy vitamin D 3 analogues possessing an ethyl at the 2 position(4 and 5), were synthesized by coupling 25 hydroxy Windaus Grundmann ketone derivative 20 with A ring synthons(15 and 19) respectively. The enantioselective synthesis of substituted bicyclic hexanes structure A ring synthons, started from all cis 3,5 dihydroxy 4 ethyl 1 (methoxycarbonyl)cyclohexane via lipase catalyzd asymmetrization, was demonstrated.展开更多
目的:探讨妊娠早、中期妇女血清25-羟维生素D-3(25-OHD-3)与妊娠期糖尿病(GDM)的关系。方法:随机选取2018年1月至2021年12月在本院进行常规孕期检查的产妇作为研究对象,孕8~12周检测其空腹血糖(FBG)及糖化血红蛋白(HbAlc)水平,孕13~15...目的:探讨妊娠早、中期妇女血清25-羟维生素D-3(25-OHD-3)与妊娠期糖尿病(GDM)的关系。方法:随机选取2018年1月至2021年12月在本院进行常规孕期检查的产妇作为研究对象,孕8~12周检测其空腹血糖(FBG)及糖化血红蛋白(HbAlc)水平,孕13~15周检测其血清25-OHD-3水平,孕24~28周行75 g口服葡萄糖耐量试验(OGTT),包括FBG、餐后1 h血糖(1 h PBG)、餐后2 h血糖(2 h PBG),并在空腹、服糖水后1 h和2 h均检测血清25-OHD-3。根据OGTT筛查结果,将其分为GDM组(n=100)及对照组(n=100)。比较2组孕早期FBG、HbAlc,孕中期血清25-OHD-3及孕中后期空腹、服糖水后1、2 h的血糖及25-OHD-3等指标的差异,并分析血清25-OHD-3水平与GDM发病的关系。结果:GDM组孕早期FBG、HbAlc及OGTT中FBG、1 h PBG、2 h PBG均高于对照组(P<0.05);GDM组血清25-OHD-3水平低于对照组,差异具有统计学意义(P<0.05);两组孕妇存在孕期血清25-OHD-3不足且缺乏的情况,血清25-OHD-3指标GDM组充足率为6.00%,对照组为7.00%;GDM组不足率为30.00%,对照组为32.00%;GDM组缺乏率为64.00%,对照组为61.00%。综合比较显示,两组血清25-OHD-3充足率、不足率及缺乏率差异无统计学意义(P>0.05);GDM组中FBG正常组的25-OHD-3水平显著高于FBG异常组(P<0.05);而服糖后1 h及2 h,血糖正常组及异常组的25-OHD-3水平比较差异无统计学意义(P>0.05)。结论:随着孕龄的增加,孕妇血清25-OHD-3水平逐渐下降,而妊娠中期较低的血清25-OHD-3水平与GDM的发生存在相关性,增加了GDM发生的风险,对于高危人群需要加强早期监测,积极补充维生素D,以预防及减少GDM的发生。展开更多
目的探讨帕金森病(parkinson's disease,PD)患者髋部骨密度(bone mineral density,BMD)、25-羟维生素D3与髋部骨折的相关性。方法以61例年龄在51~68岁的帕金森病患者为PD组,以64名年龄和性别匹配的健康人群作为对照组,采用双能X射...目的探讨帕金森病(parkinson's disease,PD)患者髋部骨密度(bone mineral density,BMD)、25-羟维生素D3与髋部骨折的相关性。方法以61例年龄在51~68岁的帕金森病患者为PD组,以64名年龄和性别匹配的健康人群作为对照组,采用双能X射线骨密度仪测定骨密度,采用电化学发光法检测25-羟维生素D3水平。对两组受试者跟踪随访3年,记录随访期间髋部骨折的发生情况并进行对比。结果 PD组髋部总骨密度、股骨颈骨密度、转子间骨密度、股骨干骨密度均低于对照组,差异具有统计学意义(t值分别为-3.747、-4.256、-4.706、-4.274,P均<0.001)。PD组25-羟维生素D3水平明显低于对照组,差异具有统计学意义(t=-4.500,P<0.001)。PD组中髋部总骨密度与25-羟维生素D3具有相关性(r=0.510,P=0.047)。PD组髋部骨折发生率高于对照组,差异有统计学意义(χ~2=4.04,P=0.04)。结论 PD患者髋部骨折发生率较同年龄、同性别健康人群高,髋部骨折发生与髋部骨密度和25-羟维生素D3水平有关。展开更多
基金Supported by the Else Kroner-Fresenius Foundation, Bad Homburg, Germany
文摘AIM： To investigate the possible involvement of 25-hydroxyvitamin D3-1cx-hydroxylase [1α-25（OH）2D3] in butyrate-induced differentiation in human intestinal cell line Caco-2 cells. METHODS： Caco-2 cells were incubated either with 3 mmol/L butyrate and 1 umol/L 25（OH）2D3 or with 1 umol/L 1α-25（OH）2D3 for various time intervals ranging from 0 to 72 h. Additionally, cells were co-incubated with butyrate and either 25（OH）2D3 or 1α-25（OH）2D3. 1α-25（OH）2D3 mRNA was determined semi-quantitatively using the fluorescent dye PicoGreen. Immunoblotting was used for the detection of 1α-25（OH）2D3 protein. Finally, enzymatic activity was measured by ELISA. RESULTS： Both butyrate and 1α-25（OH）2D3 stimulated differentiation of Caco-2 cells after a 48 h incubation period, while 25（OH）2D3 had no impact on cell differentiation. Synergistic effects on differentiation were observed when cells were co-incubated with butyrate and vitamin D metabolite. Butyrate transiently upregulated 1α-25（OH）2D3 mRNA followed by a timely delayed protein upregulation. Coincidently, enzymatic activity was enhanced significantly. The induction of the enzyme allowed for comparable differentiating effects of both vitamin D metabolites. CONCLUSION： Our experimental data provide a further mechanism for the involvement of the vitamin D signaling pathway in colonic epithelial cell differentiation by butyrate. The enhancement of 1α-25（OH）2D3 followed by antiproliferative effects of the vitamin D prohormone in the Caco-2 cell line suggest that 25（OH）2D3 in combination with butyrate may offer a new therapeutic approach forthe treatment of colon cancer.
文摘Objective To investigate the relationship between 25-hydroxy vitamin D3[25(OH)D3]level and sudomotor function(SF)in patients with type 2 diabetes mellitus(T2 DM).Methods A total of 1020 patients with T2 DM were collected in this study from Endocrinology Department in Jinling College of Clinical Medicine,Nanjing Medical University during January 2017 to December 2019.
文摘The novel 19 nor l α ,25 dihydroxy vitamin D 3 analogues possessing an ethyl at the 2 position(4 and 5), were synthesized by coupling 25 hydroxy Windaus Grundmann ketone derivative 20 with A ring synthons(15 and 19) respectively. The enantioselective synthesis of substituted bicyclic hexanes structure A ring synthons, started from all cis 3,5 dihydroxy 4 ethyl 1 (methoxycarbonyl)cyclohexane via lipase catalyzd asymmetrization, was demonstrated.
文摘目的:探讨妊娠早、中期妇女血清25-羟维生素D-3(25-OHD-3)与妊娠期糖尿病(GDM)的关系。方法:随机选取2018年1月至2021年12月在本院进行常规孕期检查的产妇作为研究对象,孕8~12周检测其空腹血糖(FBG)及糖化血红蛋白(HbAlc)水平,孕13~15周检测其血清25-OHD-3水平,孕24~28周行75 g口服葡萄糖耐量试验(OGTT),包括FBG、餐后1 h血糖(1 h PBG)、餐后2 h血糖(2 h PBG),并在空腹、服糖水后1 h和2 h均检测血清25-OHD-3。根据OGTT筛查结果,将其分为GDM组(n=100)及对照组(n=100)。比较2组孕早期FBG、HbAlc,孕中期血清25-OHD-3及孕中后期空腹、服糖水后1、2 h的血糖及25-OHD-3等指标的差异,并分析血清25-OHD-3水平与GDM发病的关系。结果:GDM组孕早期FBG、HbAlc及OGTT中FBG、1 h PBG、2 h PBG均高于对照组(P<0.05);GDM组血清25-OHD-3水平低于对照组,差异具有统计学意义(P<0.05);两组孕妇存在孕期血清25-OHD-3不足且缺乏的情况,血清25-OHD-3指标GDM组充足率为6.00%,对照组为7.00%;GDM组不足率为30.00%,对照组为32.00%;GDM组缺乏率为64.00%,对照组为61.00%。综合比较显示,两组血清25-OHD-3充足率、不足率及缺乏率差异无统计学意义(P>0.05);GDM组中FBG正常组的25-OHD-3水平显著高于FBG异常组(P<0.05);而服糖后1 h及2 h,血糖正常组及异常组的25-OHD-3水平比较差异无统计学意义(P>0.05)。结论:随着孕龄的增加,孕妇血清25-OHD-3水平逐渐下降,而妊娠中期较低的血清25-OHD-3水平与GDM的发生存在相关性,增加了GDM发生的风险,对于高危人群需要加强早期监测,积极补充维生素D,以预防及减少GDM的发生。
