目的了解局灶性脑缺血再灌注后梗死区周围微管相关蛋白(MPA)2、胶质纤维酸性蛋白(GFAP)表达的动态变化以及三七三醇皂苷对其表达的影响。方法采用改良的线栓法制备大脑中动脉阻塞0.45 h后,不同再灌注时间(1、7、14、21、28 d),大鼠短暂...目的了解局灶性脑缺血再灌注后梗死区周围微管相关蛋白(MPA)2、胶质纤维酸性蛋白(GFAP)表达的动态变化以及三七三醇皂苷对其表达的影响。方法采用改良的线栓法制备大脑中动脉阻塞0.45 h后,不同再灌注时间(1、7、14、21、28 d),大鼠短暂局灶性脑缺血模型。应用免疫双标法观察再灌注损伤后1、7、14、21、28 d Brdu、Brdu/MPA2、Brdu/GFAP表达及三七三醇皂苷对其影响。结果脑缺血再灌注后14 d Brdu表达水平达到峰值,至再灌注28 d Brdu表达水平已明显回落,但仍较正常水平高。再灌注7、14、21 d,三七三醇皂苷组Brdu/GFAP阳性细胞比例高于模型组(P<0.05)。三七三醇皂苷组Brdu/MPA2阳性细胞水平于整个实验过程中均较相同时间段对照组增多,但都无显著差异(P>0.05)。结论脑缺血再灌注后神经干细胞增殖水平增高,而三七三醇皂苷治疗可能加速了神经干细胞的增殖,并可能使增殖的神经干细胞更多向胶质细胞分化。展开更多
Background: Febrile seizures (FSs) are the most common form of childhood seizures, and genetic factors play a role in susceptibility to FS. Objective: To identify novel loci and genes associated with susceptibility to...Background: Febrile seizures (FSs) are the most common form of childhood seizures, and genetic factors play a role in susceptibility to FS. Objective: To identify novel loci and genes associated with susceptibility to FS. Methods: Study participants were the FS probands and family members of 59 Japanese nuclear families (223 members including 112 affected children). Forty- eight of these families had at least two affected children for which genome- wide linkage screening was carried out. The Genehunter software was used to perform nonparametric multipoint linkage analysis. Mutational and association analyses were conducted in all 59 Japanese FS families. Results: Genotyping data of 407 microsatellite markers suggested linkage of FSs to chromosome 18p11.2 (non- parametric linkage score = 3.68, p = 0.0001). This region includes the IMPA2 gene, which encodes myo- inositol monophosphatase (IMPase) 2. In the phosphatidylinositol- signaling pathway, IMPase is inhibited by lithium, which has a proconvulsant effect, and is stimulated by carbamazepine, an anticonvulsant. A systematic search was performed for mutations in IMPA2 in 24 unrelated randomly selected Japanese FS patients; seven variants were detected. Haplotype analysis revealed an association of a common haplotype in IMPA2 with FSs (p = 0.0009). Conclusion: The authors found a novel locus on chromosome 18p11.2 for febrile seizures (FSs). IMPA2 is likely to be an FS susceptibility gene.展开更多
文摘目的了解局灶性脑缺血再灌注后梗死区周围微管相关蛋白(MPA)2、胶质纤维酸性蛋白(GFAP)表达的动态变化以及三七三醇皂苷对其表达的影响。方法采用改良的线栓法制备大脑中动脉阻塞0.45 h后,不同再灌注时间(1、7、14、21、28 d),大鼠短暂局灶性脑缺血模型。应用免疫双标法观察再灌注损伤后1、7、14、21、28 d Brdu、Brdu/MPA2、Brdu/GFAP表达及三七三醇皂苷对其影响。结果脑缺血再灌注后14 d Brdu表达水平达到峰值,至再灌注28 d Brdu表达水平已明显回落,但仍较正常水平高。再灌注7、14、21 d,三七三醇皂苷组Brdu/GFAP阳性细胞比例高于模型组(P<0.05)。三七三醇皂苷组Brdu/MPA2阳性细胞水平于整个实验过程中均较相同时间段对照组增多,但都无显著差异(P>0.05)。结论脑缺血再灌注后神经干细胞增殖水平增高,而三七三醇皂苷治疗可能加速了神经干细胞的增殖,并可能使增殖的神经干细胞更多向胶质细胞分化。
文摘Background: Febrile seizures (FSs) are the most common form of childhood seizures, and genetic factors play a role in susceptibility to FS. Objective: To identify novel loci and genes associated with susceptibility to FS. Methods: Study participants were the FS probands and family members of 59 Japanese nuclear families (223 members including 112 affected children). Forty- eight of these families had at least two affected children for which genome- wide linkage screening was carried out. The Genehunter software was used to perform nonparametric multipoint linkage analysis. Mutational and association analyses were conducted in all 59 Japanese FS families. Results: Genotyping data of 407 microsatellite markers suggested linkage of FSs to chromosome 18p11.2 (non- parametric linkage score = 3.68, p = 0.0001). This region includes the IMPA2 gene, which encodes myo- inositol monophosphatase (IMPase) 2. In the phosphatidylinositol- signaling pathway, IMPase is inhibited by lithium, which has a proconvulsant effect, and is stimulated by carbamazepine, an anticonvulsant. A systematic search was performed for mutations in IMPA2 in 24 unrelated randomly selected Japanese FS patients; seven variants were detected. Haplotype analysis revealed an association of a common haplotype in IMPA2 with FSs (p = 0.0009). Conclusion: The authors found a novel locus on chromosome 18p11.2 for febrile seizures (FSs). IMPA2 is likely to be an FS susceptibility gene.
