Background Cotton fiber is a model tissue for studying microtubule-associated proteins(MAPs).The Xklp2(TPX2)proteins that belong to the novel MAPs member mainly participate in the formation and development of microtub...Background Cotton fiber is a model tissue for studying microtubule-associated proteins(MAPs).The Xklp2(TPX2)proteins that belong to the novel MAPs member mainly participate in the formation and development of microtubule(MT).However,there is a lack of studies concerning the systematic characterization of the TPX2 genes family in cotton.Therefore,the identification and portrayal of G.hirsutum TPX2 genes can provide key targets for molecular manipula-tion in the breeding of cotton fiber improvement.Result In this study,TPX2 family genes were classified into two distinct subclasses TPXLs and MAP genes WAVE DAMP-ENED2-LIKE(WDLs)and quite conservative in quantity.GhWDL3 was significantly up-regulated in 15 days post anthe-sis fibers of ZRI-015(an upland cotton with longer and stronger fiber).GhWDL3 promotes all stem hairs to become straight when overexpressed in Arabidopsis,which may indirectly regulate cotton fiber cell morphology during fiber development.Virus induced gene silencing(VIGS)results showed that GhWDL3 inhibited fiber cell elongation at fiber development periods through regulating the expression of cell wall related genes.Conclusion These results reveal that GhWDL3 regulated cotton fiber cell elongation and provide crucial information for the further investigation in the regulatory mechanisms/networks of cotton fiber length.展开更多
Electrocatalytic overall water splitting(OWS),a pivotal approach in addressing the global energy crisis,aims to produce hydrogen and oxygen.However,most of the catalysts in powder form are adhesively bounding to the e...Electrocatalytic overall water splitting(OWS),a pivotal approach in addressing the global energy crisis,aims to produce hydrogen and oxygen.However,most of the catalysts in powder form are adhesively bounding to the electrodes,resulting in catalyst detachment by bubble generation and other uncertain interference,and eventually reducing the OWS performance.To surmount this challenge,we synthesized a hybrid material of Co_(3)S_(4)-pyrolysis lotus fiber(labeled as Co_(3)S_(4)-p LF)textile by hydrothermal and hightemperature pyrolysis processes for electrocatalytic OWS.Owing to the natural LF textile exposing the uniformly distributed functional groups(AOH,ANH_(2),etc.)to anchor Co_(3)S_(4)nanoparticles with hierarchical porous structure and outstanding hydrophily,the hybrid Co_(3)S_(4)-p LF catalyst shows low overpotentials at 10 m A cm^(-2)(η_(10,HER)=100 m Vη_(10,OER)=240 mV)alongside prolonged operational stability during electrocatalytic reactions.Theoretical calculations reveal that the electron transfer from p LF to Co_(3)S_(4)in the hybrid Co_(3)S_(4)-p LF is beneficial to the electrocatalytic process.This work will shed light on the development of nature-inspired carbon-based materials in hybrid electrocatalysts for OWS.展开更多
Electrochemical detection of 3-methyl-4-nitrophenol (MNP) in direct phenol oxidation occurs at high potentials and generally leads to progressive passivation of the electrochemical sensor. This study describes the use...Electrochemical detection of 3-methyl-4-nitrophenol (MNP) in direct phenol oxidation occurs at high potentials and generally leads to progressive passivation of the electrochemical sensor. This study describes the use of a carbon fiber microelectrode modified with a tetrasulfonated nickel phthalocyanine complex for the detection of MNP at a lower potential than that of direct phenol oxidation. The MNP voltammogram showed the presence of an anodic peak at -0.11 V vs SCE, corresponding to the oxidation of the hydroxylamine group generated after the reduction of the nitro group. The effect of buffer pH on the peak current and SWV parameters such as frequency, scan increment, and pulse amplitude were studied and optimized to have better electrochemical response of the proposed sensor. With these optimal parameters, the calibration curve shows that the peak current varied linearly as a function of MNP concentration, leading to a limit of detection (LoD) of 1.1 μg/L. These results show an appreciable sensitivity of the sensor for detecting the MNP at relatively low potentials, making it possible to avoid passivation phenomena.展开更多
A novel distributed weak fiber Bragg gratings （FBGs） vibration sensing system has been designed to overcome the disadvantages of the conventional methods for optical fiber sensing networking, which are： low signal ...A novel distributed weak fiber Bragg gratings （FBGs） vibration sensing system has been designed to overcome the disadvantages of the conventional methods for optical fiber sensing networking, which are： low signal intensity in the usually adopted time-division multiplexing （TDM） technology, insufficient quantity of multiplexed FBGs in the wavelength-division multiplexing （WDM） technology, and that the mixed WDM/TDM technology measures only the physical parameters of the FBG locations but cannot perform distributed measurement over the whole optical fiber. This novel system determines vibration events in the optical fiber line according to the intensity variation of the interference signals between the adjacent weak FBG reflected signals and locates the vibration points accurately using the TDM technology. It has been proven by tests that this system performs vibration signal detection and demodulation in a way more convenient than the conventional methods for the optical fiber sensing system. It also measures over the whole optical fiber, therefore, distributed measurement is fulfilled, and the system locating accuracy is up to 20m, capable of detecting any signals of whose drive signals lower limit voltage is 0.2 V while the frequency range is 3 Hz - 1 000 Hz. The system has the great practical significance and application value for perimeter surveillance systems.展开更多
本研究旨在通过TB Green Real-time PCR的方法对国内6个省(四川省、山西省、河南省、河北省、江苏省和内蒙古自治区)的9个规模化养牛场140份以流鼻涕、发热、咳嗽、呼吸困难为特征的病牛呼吸道鼻拭子样本开展牛腺病毒3型(bovine adenovi...本研究旨在通过TB Green Real-time PCR的方法对国内6个省(四川省、山西省、河南省、河北省、江苏省和内蒙古自治区)的9个规模化养牛场140份以流鼻涕、发热、咳嗽、呼吸困难为特征的病牛呼吸道鼻拭子样本开展牛腺病毒3型(bovine adenovirus type 3,BAdV-3)感染状况调查,并对其中的阳性样本进行fiber轴区基因检测。结果显示,140份样品中BAdV-3阳性率为36.4%,除河北省以外,其余各省均有BAdV-3检出,其中,四川及河南省检出率最高,分别达到82.9%(29/35)和83.3%(10/12)。其中,传统型占总阳性比的80.4%,fiber轴区缺失毒株占总阳性样本的19.6%。缺失位置与数量一致,轴区连续缺失237个碱基对(79个氨基酸),且缺失型仅在四川和河南两省检出,检出率分别为24.1%(7/29)和30%(3/10),场群阳性率分别为100%(2/2)和100%(1/1)。结果表明,BAdV-3已在我国牛场中广泛分布,目前流行的主要以传统型毒株为主,fiber轴区缺失毒株在我国具有独特的地域分布,主要在四川和河南省流行。展开更多
基金supported by the National Key Research and Development Program of China(2022YFD1200300)China Agriculture Research System(CARS-15-01).
文摘Background Cotton fiber is a model tissue for studying microtubule-associated proteins(MAPs).The Xklp2(TPX2)proteins that belong to the novel MAPs member mainly participate in the formation and development of microtubule(MT).However,there is a lack of studies concerning the systematic characterization of the TPX2 genes family in cotton.Therefore,the identification and portrayal of G.hirsutum TPX2 genes can provide key targets for molecular manipula-tion in the breeding of cotton fiber improvement.Result In this study,TPX2 family genes were classified into two distinct subclasses TPXLs and MAP genes WAVE DAMP-ENED2-LIKE(WDLs)and quite conservative in quantity.GhWDL3 was significantly up-regulated in 15 days post anthe-sis fibers of ZRI-015(an upland cotton with longer and stronger fiber).GhWDL3 promotes all stem hairs to become straight when overexpressed in Arabidopsis,which may indirectly regulate cotton fiber cell morphology during fiber development.Virus induced gene silencing(VIGS)results showed that GhWDL3 inhibited fiber cell elongation at fiber development periods through regulating the expression of cell wall related genes.Conclusion These results reveal that GhWDL3 regulated cotton fiber cell elongation and provide crucial information for the further investigation in the regulatory mechanisms/networks of cotton fiber length.
基金supported by the Scientific Research Foundation of Hunan Provincial Education Department,China(22B0893)the Scientific Research Foundation of Hunan Provincial Education Department,China(20A060)。
文摘Electrocatalytic overall water splitting(OWS),a pivotal approach in addressing the global energy crisis,aims to produce hydrogen and oxygen.However,most of the catalysts in powder form are adhesively bounding to the electrodes,resulting in catalyst detachment by bubble generation and other uncertain interference,and eventually reducing the OWS performance.To surmount this challenge,we synthesized a hybrid material of Co_(3)S_(4)-pyrolysis lotus fiber(labeled as Co_(3)S_(4)-p LF)textile by hydrothermal and hightemperature pyrolysis processes for electrocatalytic OWS.Owing to the natural LF textile exposing the uniformly distributed functional groups(AOH,ANH_(2),etc.)to anchor Co_(3)S_(4)nanoparticles with hierarchical porous structure and outstanding hydrophily,the hybrid Co_(3)S_(4)-p LF catalyst shows low overpotentials at 10 m A cm^(-2)(η_(10,HER)=100 m Vη_(10,OER)=240 mV)alongside prolonged operational stability during electrocatalytic reactions.Theoretical calculations reveal that the electron transfer from p LF to Co_(3)S_(4)in the hybrid Co_(3)S_(4)-p LF is beneficial to the electrocatalytic process.This work will shed light on the development of nature-inspired carbon-based materials in hybrid electrocatalysts for OWS.
文摘Electrochemical detection of 3-methyl-4-nitrophenol (MNP) in direct phenol oxidation occurs at high potentials and generally leads to progressive passivation of the electrochemical sensor. This study describes the use of a carbon fiber microelectrode modified with a tetrasulfonated nickel phthalocyanine complex for the detection of MNP at a lower potential than that of direct phenol oxidation. The MNP voltammogram showed the presence of an anodic peak at -0.11 V vs SCE, corresponding to the oxidation of the hydroxylamine group generated after the reduction of the nitro group. The effect of buffer pH on the peak current and SWV parameters such as frequency, scan increment, and pulse amplitude were studied and optimized to have better electrochemical response of the proposed sensor. With these optimal parameters, the calibration curve shows that the peak current varied linearly as a function of MNP concentration, leading to a limit of detection (LoD) of 1.1 μg/L. These results show an appreciable sensitivity of the sensor for detecting the MNP at relatively low potentials, making it possible to avoid passivation phenomena.
基金This work is supported by the Major Program of the National Natural Science Foundation of China （Grant No. 61290311）.
文摘A novel distributed weak fiber Bragg gratings （FBGs） vibration sensing system has been designed to overcome the disadvantages of the conventional methods for optical fiber sensing networking, which are： low signal intensity in the usually adopted time-division multiplexing （TDM） technology, insufficient quantity of multiplexed FBGs in the wavelength-division multiplexing （WDM） technology, and that the mixed WDM/TDM technology measures only the physical parameters of the FBG locations but cannot perform distributed measurement over the whole optical fiber. This novel system determines vibration events in the optical fiber line according to the intensity variation of the interference signals between the adjacent weak FBG reflected signals and locates the vibration points accurately using the TDM technology. It has been proven by tests that this system performs vibration signal detection and demodulation in a way more convenient than the conventional methods for the optical fiber sensing system. It also measures over the whole optical fiber, therefore, distributed measurement is fulfilled, and the system locating accuracy is up to 20m, capable of detecting any signals of whose drive signals lower limit voltage is 0.2 V while the frequency range is 3 Hz - 1 000 Hz. The system has the great practical significance and application value for perimeter surveillance systems.
文摘本研究旨在通过TB Green Real-time PCR的方法对国内6个省(四川省、山西省、河南省、河北省、江苏省和内蒙古自治区)的9个规模化养牛场140份以流鼻涕、发热、咳嗽、呼吸困难为特征的病牛呼吸道鼻拭子样本开展牛腺病毒3型(bovine adenovirus type 3,BAdV-3)感染状况调查,并对其中的阳性样本进行fiber轴区基因检测。结果显示,140份样品中BAdV-3阳性率为36.4%,除河北省以外,其余各省均有BAdV-3检出,其中,四川及河南省检出率最高,分别达到82.9%(29/35)和83.3%(10/12)。其中,传统型占总阳性比的80.4%,fiber轴区缺失毒株占总阳性样本的19.6%。缺失位置与数量一致,轴区连续缺失237个碱基对(79个氨基酸),且缺失型仅在四川和河南两省检出,检出率分别为24.1%(7/29)和30%(3/10),场群阳性率分别为100%(2/2)和100%(1/1)。结果表明,BAdV-3已在我国牛场中广泛分布,目前流行的主要以传统型毒株为主,fiber轴区缺失毒株在我国具有独特的地域分布,主要在四川和河南省流行。
