Using a modification of the very sensitive glucose-DAB-nickel(GDN)immunohistochemistry method,FOS(the expression protein of the oncogene c-fos)-,neuropeptide Y(NPY)-,somatostatin(SOM)-,leu-enkephalin(L-Enk),cholecysto...Using a modification of the very sensitive glucose-DAB-nickel(GDN)immunohistochemistry method,FOS(the expression protein of the oncogene c-fos)-,neuropeptide Y(NPY)-,somatostatin(SOM)-,leu-enkephalin(L-Enk),cholecystokinin(CCK)-,neurotensin(NT)-,and tyrosine hydroxylase(TH)-immunoreactivities were firstdemonstrated in the protozoan,Stylonychia mytilus.The GDN method and expression ofFOS,neuropeptides and TH in the Stylonychia mytilus were discussed.展开更多
Background: Myeloperoxidase staining is used to differentiate leukemias since several decades. Despite implementation of flow cytometric, cytogenetic and molecular techniques for identification of leukemic blasts, his...Background: Myeloperoxidase staining is used to differentiate leukemias since several decades. Despite implementation of flow cytometric, cytogenetic and molecular techniques for identification of leukemic blasts, histochemical stains such as myeloperoxidase stain are persistently used for better classification of leukemias. The myeloperoxidase staining is a time consuming and hazardous procedure. The present report describes a sensitive, rapid and easy method for assessment of peroxidase activity. Materials and Methods: Bone marrow aspiration slides were stained with Dako product: Code number: K3467 containing DAB chromogen (3,3-diaminobenzidine in chromogen solution) and substrate buffer (Imidasole-HCL buffer, PH 7.5 containing hydrogen peroxide and an anti microbial agent) in a rapid procedure taking only ten minutes time. The staining needs no material preparation steps. Neutrophils in the slide are taken as positive control or another normal smear was costained to be used as control. All cases were followed up with flow cytometry and cytogenetic studies. Result: The reaction product of this stain is brown and granular. Promyelocytes and myelocytes are the most strongly staining cells with positive (primary) granules. Lymphoblasts are negative. The result of classification of leukemias with this technique was in concordance with flow cytometric immunophenotyping. Discussion: Many practical techniques have been described using benzidine as an indicator for myeloperoxidase staining. Benzidine is a carcinogenic material and its usage is severely restricted in laboratory. Formerly we prepared requisite materials for myeloperoxidase staining by hazardous ways (boiling), but we decided to apply ready to use 3,3-diaminobenzidine (DAB), which is used in final step of immunohistochemistry stains. Conclusion: Use of 3,3-diaminobenzidine (DAB) is highly recommended for myeloperoxidase staining, while the result is extraordinary and fully compatible with flow cytometry and the method is safe and rapid.展开更多
A simple vortex-assisted cloud-point extraction(VA-CPE)method combined with the fluorescence(FL)strategy was developed to extract and determine inorganic Se(IV)in environmental water.Toluene was used as an extraction ...A simple vortex-assisted cloud-point extraction(VA-CPE)method combined with the fluorescence(FL)strategy was developed to extract and determine inorganic Se(IV)in environmental water.Toluene was used as an extraction reagent,and Se(VI)was reduced to Se(IV)by subjecting its solution in 4 mol/L HCl to microwave heating for generating a yellow piazselenol complex using 3,3'-diaminobenzidine(DAB)and Se(IV).The concentration of the complex exhibited an excellent linear relation with the FL at 560 nm with an excitation wavelength of 420 nm.In addition,the effects of the toluene volume,pH,and VA-CPE parameters were investigated and the interference from coexisting ions was also studied.This method was successfully applied to determine the concentration selenium in environmental water samples with a detection limit of 0.05μg/L in the line range from 0.50μg/L to 50.00μg/L.展开更多
文摘Using a modification of the very sensitive glucose-DAB-nickel(GDN)immunohistochemistry method,FOS(the expression protein of the oncogene c-fos)-,neuropeptide Y(NPY)-,somatostatin(SOM)-,leu-enkephalin(L-Enk),cholecystokinin(CCK)-,neurotensin(NT)-,and tyrosine hydroxylase(TH)-immunoreactivities were firstdemonstrated in the protozoan,Stylonychia mytilus.The GDN method and expression ofFOS,neuropeptides and TH in the Stylonychia mytilus were discussed.
文摘Background: Myeloperoxidase staining is used to differentiate leukemias since several decades. Despite implementation of flow cytometric, cytogenetic and molecular techniques for identification of leukemic blasts, histochemical stains such as myeloperoxidase stain are persistently used for better classification of leukemias. The myeloperoxidase staining is a time consuming and hazardous procedure. The present report describes a sensitive, rapid and easy method for assessment of peroxidase activity. Materials and Methods: Bone marrow aspiration slides were stained with Dako product: Code number: K3467 containing DAB chromogen (3,3-diaminobenzidine in chromogen solution) and substrate buffer (Imidasole-HCL buffer, PH 7.5 containing hydrogen peroxide and an anti microbial agent) in a rapid procedure taking only ten minutes time. The staining needs no material preparation steps. Neutrophils in the slide are taken as positive control or another normal smear was costained to be used as control. All cases were followed up with flow cytometry and cytogenetic studies. Result: The reaction product of this stain is brown and granular. Promyelocytes and myelocytes are the most strongly staining cells with positive (primary) granules. Lymphoblasts are negative. The result of classification of leukemias with this technique was in concordance with flow cytometric immunophenotyping. Discussion: Many practical techniques have been described using benzidine as an indicator for myeloperoxidase staining. Benzidine is a carcinogenic material and its usage is severely restricted in laboratory. Formerly we prepared requisite materials for myeloperoxidase staining by hazardous ways (boiling), but we decided to apply ready to use 3,3-diaminobenzidine (DAB), which is used in final step of immunohistochemistry stains. Conclusion: Use of 3,3-diaminobenzidine (DAB) is highly recommended for myeloperoxidase staining, while the result is extraordinary and fully compatible with flow cytometry and the method is safe and rapid.
基金Scientific Research Staring Foundation for the Returned Overseas Chinese Scholars,Ministry of Education of China(No.[2015] 1098)Cooperation of Production and Research Foundation of Science and Technology Bureau in Yulin,Shaanxi Province,China(No.2016CXY-02)+1 种基金Key Science and Technology of Program of Shaanxi Province,China(No.2017GY-131)Foundation of Yulin University for the Introduction of Talents,China(Nos.14GK23,17GK15)
文摘A simple vortex-assisted cloud-point extraction(VA-CPE)method combined with the fluorescence(FL)strategy was developed to extract and determine inorganic Se(IV)in environmental water.Toluene was used as an extraction reagent,and Se(VI)was reduced to Se(IV)by subjecting its solution in 4 mol/L HCl to microwave heating for generating a yellow piazselenol complex using 3,3'-diaminobenzidine(DAB)and Se(IV).The concentration of the complex exhibited an excellent linear relation with the FL at 560 nm with an excitation wavelength of 420 nm.In addition,the effects of the toluene volume,pH,and VA-CPE parameters were investigated and the interference from coexisting ions was also studied.This method was successfully applied to determine the concentration selenium in environmental water samples with a detection limit of 0.05μg/L in the line range from 0.50μg/L to 50.00μg/L.
