A real-time fluorescent quantitative immuno-polymerase chain reaction （RT-IPCR） assay was developed for the detection of non-dioxin- like polychlorinated biphenyl （PCB） congener in soil samples. Based on the const...A real-time fluorescent quantitative immuno-polymerase chain reaction （RT-IPCR） assay was developed for the detection of non-dioxin- like polychlorinated biphenyl （PCB） congener in soil samples. Based on the construction of 3,4-dichlorinated biphenyl （IUPAC PCB 12） hapten and its immunogen, the specific polyclonal antibodies （pAbs） to PCB12 was obtained and used to develop a direct competitive RT-IPCR assay. Using the optimized assay, a standard curve for PCB 12 was prepared. The linear range for the determination of PCB 12 was from 10.0 to 1.0 x l06 fg/mL with a correlation coefficient of 0.98 and a detection limit of 1.53 fg/rnL. The RT-IPCR assays were tested for their cross-reactivity profiles using four selected congeners and four Aroclor products. The results for the soil samples correlated with the concentrations of PCBs obtained by gas chromatography/mass spectrometry. This highly specific, sensitive, and robust assay can be applied to on-site tests of PCBs and serve as a model for other pollutant immunoassays.展开更多
基金supported by the National Natural Science Foundation of China (No. 21177082)the Science and Technology Commission of Shanghai Municipality in China (Key Project of Fundamental Research,No.09JC1407600)+1 种基金the Shanghai Jiao Tong University science and technology innovation special fund development projectsthe Postdoctoral Science Foundation of Shanghai (No. 11R21414600)
文摘A real-time fluorescent quantitative immuno-polymerase chain reaction （RT-IPCR） assay was developed for the detection of non-dioxin- like polychlorinated biphenyl （PCB） congener in soil samples. Based on the construction of 3,4-dichlorinated biphenyl （IUPAC PCB 12） hapten and its immunogen, the specific polyclonal antibodies （pAbs） to PCB12 was obtained and used to develop a direct competitive RT-IPCR assay. Using the optimized assay, a standard curve for PCB 12 was prepared. The linear range for the determination of PCB 12 was from 10.0 to 1.0 x l06 fg/mL with a correlation coefficient of 0.98 and a detection limit of 1.53 fg/rnL. The RT-IPCR assays were tested for their cross-reactivity profiles using four selected congeners and four Aroclor products. The results for the soil samples correlated with the concentrations of PCBs obtained by gas chromatography/mass spectrometry. This highly specific, sensitive, and robust assay can be applied to on-site tests of PCBs and serve as a model for other pollutant immunoassays.
