This study aimed to investigate the role of insulin-like growth factor-binding protein-3 （IGFBP-3） in erectUe dysfunction （ED） in two-kidney one-clip （2K-1C） hypertensive rats treated with the β-blocking agent ...This study aimed to investigate the role of insulin-like growth factor-binding protein-3 （IGFBP-3） in erectUe dysfunction （ED） in two-kidney one-clip （2K-1C） hypertensive rats treated with the β-blocking agent propranolol. Adult male Wistar rats were randomly divided into three groups： a normal control group, a hypertensive control group and a propranolol treatment group （n=9）. After 4 weeks of propranolol treatment, intracavemous pressure （ICP） responses to electrical stimulation of the cavernous nerves were evaluated. The expression of IGFBP-3 and insulin-like growth factor-1 （IGF-1） mRNA and protein in the rat cavernous tissue were detected by quantitative real-time PCR and Western blot, respectively. The concentration of cyclic guanosine monophosphate （cGMP） in the cavernous tissue was determined by enzyme-linked immunosorbent assay （ELISA）. Cavernosal pressure in response to cavernous nerve stimulation was decreased 4 weeks after propranolol treatment （P〈0.01, compared to the hypertensive control group）. IGFBP-3 mRNA and protein expression was increased in the propranolol treatment group compared to the hypertensive control group （P〈O.01）, whereas IGF-1 expression was decreased in the propranolol treatment group compared to the hypertensive control group （P〈0.01）. In addition, cavernous cGMP concentration was decreased in the prepranolol treatment group compared to the hypertensive control group （P〈0.01）. Taken together, these results suggest that the upregulation of IGFBP-3 may play a role in the development of ED in hypertensive rats.展开更多
A systematic method was developed for ice-class propeller modeling,performance estimation,strength and integrity evaluation and optimization.To estimate the impact of sea ice on the propeller structure,URI3 rules,esta...A systematic method was developed for ice-class propeller modeling,performance estimation,strength and integrity evaluation and optimization.To estimate the impact of sea ice on the propeller structure,URI3 rules,established by the International Association of Classification Societies in 2007,were applied for ice loading calculations.An R-class propeller(a type of ice-class propeller)was utilized for subsequent investigations.The propeller modeling was simplified based on a conventional method,which expedited the model building process.The propeller performance was simulated using the computational fluid dynamics(CFD)method.The simulation results were validated by comparison with experimental data.Furthermore,the hydrodynamic pressure was transferred into a finite element analysis(FEA)module for strength assessment of ice-class propellers.According to URI3 rules,the ice loading was estimated based on different polar classes and working cases.Then,the FEA method was utilized to evaluate the propeller strength.The validation showed that the simulation results accorded with recent research results.Finally,an improved optimization method was developed to save the propeller constituent materials.The optimized propeller example had a minimum safety factor of 1.55,satisfying the safety factor requirement of≥1.5,and reduced the design volume to 88.2%of the original.展开更多
This article introduces a change rule of 3x + 1 problem (Collatz conjecture), it’s named LiKe’s Rule. It’s a map of 3x + 1 problem, and details the path of each step of the change: For any positive integer, change ...This article introduces a change rule of 3x + 1 problem (Collatz conjecture), it’s named LiKe’s Rule. It’s a map of 3x + 1 problem, and details the path of each step of the change: For any positive integer, change by the Collatz conjecture. 1) This positive integer will change to an odd number;2) The odd number must change to a number of LiKe’s second sequence {3<sup>n</sup> – 1| n ∈ Z<sup>+</sup>};3) Then this 3<sup>n</sup> - 1 will change to a smaller 3<sup>n </sup>– 1 and gradually decrease to 8 (that is 3<sup>2</sup> - 1) then back to 1 in the end. If we can determine each step, the Collatz conjecture will be true. This is certainly more valuable than 2<sup>n</sup> (it might even explain 2<sup>n</sup>). And to illustrate the importance of this rule, introduced some important funny corollaries related to it.展开更多
基金ACKNOWLEDGMENTS We thank Prof. B. W. Peng and Dr S. R, Guo for their excellent technical support. This study was supported by the National Natural Science Foundation of China （no. 30872572）.
文摘This study aimed to investigate the role of insulin-like growth factor-binding protein-3 （IGFBP-3） in erectUe dysfunction （ED） in two-kidney one-clip （2K-1C） hypertensive rats treated with the β-blocking agent propranolol. Adult male Wistar rats were randomly divided into three groups： a normal control group, a hypertensive control group and a propranolol treatment group （n=9）. After 4 weeks of propranolol treatment, intracavemous pressure （ICP） responses to electrical stimulation of the cavernous nerves were evaluated. The expression of IGFBP-3 and insulin-like growth factor-1 （IGF-1） mRNA and protein in the rat cavernous tissue were detected by quantitative real-time PCR and Western blot, respectively. The concentration of cyclic guanosine monophosphate （cGMP） in the cavernous tissue was determined by enzyme-linked immunosorbent assay （ELISA）. Cavernosal pressure in response to cavernous nerve stimulation was decreased 4 weeks after propranolol treatment （P〈0.01, compared to the hypertensive control group）. IGFBP-3 mRNA and protein expression was increased in the propranolol treatment group compared to the hypertensive control group （P〈O.01）, whereas IGF-1 expression was decreased in the propranolol treatment group compared to the hypertensive control group （P〈0.01）. In addition, cavernous cGMP concentration was decreased in the prepranolol treatment group compared to the hypertensive control group （P〈0.01）. Taken together, these results suggest that the upregulation of IGFBP-3 may play a role in the development of ED in hypertensive rats.
基金The author would like to thank University of Tasmania and Newcastle University for their support。
文摘A systematic method was developed for ice-class propeller modeling,performance estimation,strength and integrity evaluation and optimization.To estimate the impact of sea ice on the propeller structure,URI3 rules,established by the International Association of Classification Societies in 2007,were applied for ice loading calculations.An R-class propeller(a type of ice-class propeller)was utilized for subsequent investigations.The propeller modeling was simplified based on a conventional method,which expedited the model building process.The propeller performance was simulated using the computational fluid dynamics(CFD)method.The simulation results were validated by comparison with experimental data.Furthermore,the hydrodynamic pressure was transferred into a finite element analysis(FEA)module for strength assessment of ice-class propellers.According to URI3 rules,the ice loading was estimated based on different polar classes and working cases.Then,the FEA method was utilized to evaluate the propeller strength.The validation showed that the simulation results accorded with recent research results.Finally,an improved optimization method was developed to save the propeller constituent materials.The optimized propeller example had a minimum safety factor of 1.55,satisfying the safety factor requirement of≥1.5,and reduced the design volume to 88.2%of the original.
文摘This article introduces a change rule of 3x + 1 problem (Collatz conjecture), it’s named LiKe’s Rule. It’s a map of 3x + 1 problem, and details the path of each step of the change: For any positive integer, change by the Collatz conjecture. 1) This positive integer will change to an odd number;2) The odd number must change to a number of LiKe’s second sequence {3<sup>n</sup> – 1| n ∈ Z<sup>+</sup>};3) Then this 3<sup>n</sup> - 1 will change to a smaller 3<sup>n </sup>– 1 and gradually decrease to 8 (that is 3<sup>2</sup> - 1) then back to 1 in the end. If we can determine each step, the Collatz conjecture will be true. This is certainly more valuable than 2<sup>n</sup> (it might even explain 2<sup>n</sup>). And to illustrate the importance of this rule, introduced some important funny corollaries related to it.
