AIM: To examine 5′ and 3′ terminal sequences of hepatitis E virus (HEV) isolated from Morocco, to confirm 5′ methylated cap structure of the genome, and to investigate whether the 3′ UTR can be used to distinguish...AIM: To examine 5′ and 3′ terminal sequences of hepatitis E virus (HEV) isolated from Morocco, to confirm 5′ methylated cap structure of the genome, and to investigate whether the 3′ UTR can be used to distinguish HEV genotypes instead of HEV complete genome sequence.METHODS: RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE) was employed to obtain the 5′ and 3′ terminal sequences of HEV Morocco strain. The 3′ UTR sequence of the Morocco strain was compared with that of the other 29 HEV strains using the DNAStar software.RESULTS: The 5′ PCR product was obtained only from the RLM-RACE based on the capped RNA template. The 5′ UTR of the Morocco strain had 26 nucleotides, and the 3′ UTR had 65 nucleotides upstream to the polyA. The 5′ UTR between HEV strains had only point mutations of nucleotides.The phylogenetic tree based on the sequences of 3′ UTR was not the same as that based on the complete sequences.CONCLUSION: The genome of HEV Morocco strain was methylated cap structure. The 3′ terminal sequence can not be used for distinguishing HEV genotype for all HEV strains in place of the whole HEV genome sequence.展开更多
The assays for bovine immunodeficiency virus (BIV) induced syncytium formation and BIV long terminal repeat (LTR) directed luciferase (Luc) gene expression were applied to screen and evaluate anti AIDS drugs. Frequen...The assays for bovine immunodeficiency virus (BIV) induced syncytium formation and BIV long terminal repeat (LTR) directed luciferase (Luc) gene expression were applied to screen and evaluate anti AIDS drugs. Frequency of the syncytium formation and BIV LTR directed Luc activity were in proportion to the number of input BIV infected cells. AZT inhibited the syncytium formation and the BIV LTR directed Luc gene expression level. Its inhibitory effects were dosedependent with the IC 50 being 0.24 and 0.052 mmol / L, respectively.展开更多
基金Supported by the Natural Scientific Foundation,No.30271231 and the Natural Scientific Foundation of Jiangsu Province,No.BK2002053
文摘AIM: To examine 5′ and 3′ terminal sequences of hepatitis E virus (HEV) isolated from Morocco, to confirm 5′ methylated cap structure of the genome, and to investigate whether the 3′ UTR can be used to distinguish HEV genotypes instead of HEV complete genome sequence.METHODS: RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE) was employed to obtain the 5′ and 3′ terminal sequences of HEV Morocco strain. The 3′ UTR sequence of the Morocco strain was compared with that of the other 29 HEV strains using the DNAStar software.RESULTS: The 5′ PCR product was obtained only from the RLM-RACE based on the capped RNA template. The 5′ UTR of the Morocco strain had 26 nucleotides, and the 3′ UTR had 65 nucleotides upstream to the polyA. The 5′ UTR between HEV strains had only point mutations of nucleotides.The phylogenetic tree based on the sequences of 3′ UTR was not the same as that based on the complete sequences.CONCLUSION: The genome of HEV Morocco strain was methylated cap structure. The 3′ terminal sequence can not be used for distinguishing HEV genotype for all HEV strains in place of the whole HEV genome sequence.
文摘The assays for bovine immunodeficiency virus (BIV) induced syncytium formation and BIV long terminal repeat (LTR) directed luciferase (Luc) gene expression were applied to screen and evaluate anti AIDS drugs. Frequency of the syncytium formation and BIV LTR directed Luc activity were in proportion to the number of input BIV infected cells. AZT inhibited the syncytium formation and the BIV LTR directed Luc gene expression level. Its inhibitory effects were dosedependent with the IC 50 being 0.24 and 0.052 mmol / L, respectively.
