In order to Isolate anti-human Immunodeflclency virus （HIV） agents from natural products, 97 ethanollc extracts of 90 fungi were tested for their Inhibitory activity on HIV-1. Most of the extracts tested were relati...In order to Isolate anti-human Immunodeflclency virus （HIV） agents from natural products, 97 ethanollc extracts of 90 fungi were tested for their Inhibitory activity on HIV-1. Most of the extracts tested were relatively non-toxic to human lymphocytic MT-4 cells, but extracts of some fungi exhibited potent antl-HIV activity In an in vitro 3-（4, 5-dlmethyl-2 thlazoyl）-2,5-dlphenyl-2H-tetrazollum bromide assay with a selectivity Index greater than 3. Most fungi were Isolated from Dendrobium sp. and Taxus sp.展开更多
Objective：To investigate the anticancer activity of the total flavonoids isolated from a herbal formula,Xianhe Yanling Recipe（仙鹤延龄方）,a recipe commonly used in cancer patients in China.Methods： The in vitro an...Objective：To investigate the anticancer activity of the total flavonoids isolated from a herbal formula,Xianhe Yanling Recipe（仙鹤延龄方）,a recipe commonly used in cancer patients in China.Methods： The in vitro anticancer activity of the total flavonoids was determined using the 3-（4,5-dimethylthiazole-2-yl）-2,5- diphenyltetrazolium bromide（MTT） assay on three cancer cell lines：MCF-7（a human breast adenocarcinoma cell line）,HepG-2（a human hepatocellular carcinoma cell line） and ES-2（a human ovarian cancer cell line）. The in vivo anticancer effect of the total flavonoids was assessed in a mouse tumor model bearing H22-induced hepatocellular carcinoma,and cisplatin was used as a positive control.Results：The total flavonoids exerted a powerful inhibitory effect on the three cell lines,with 50%inhibiting concentrations（IC_（50）） of 24.948,31.569 and 6.923μg/mL,respectively.In vivo studies showed that the total flavonoids had dose-dependent inhibitory effects on hepatocellular carcinoma in mice.Conclusion：The total flavonoids from Xianhe Yanling Recipe have potential anticancer activity,and further researches and development are warranted.展开更多
基金National Medicine Science Foundation during the 10th Five-Year Plan Period （2001BA705B01） and the National Science Fund for Distinguished Young Scholars （30325047）.
文摘In order to Isolate anti-human Immunodeflclency virus （HIV） agents from natural products, 97 ethanollc extracts of 90 fungi were tested for their Inhibitory activity on HIV-1. Most of the extracts tested were relatively non-toxic to human lymphocytic MT-4 cells, but extracts of some fungi exhibited potent antl-HIV activity In an in vitro 3-（4, 5-dlmethyl-2 thlazoyl）-2,5-dlphenyl-2H-tetrazollum bromide assay with a selectivity Index greater than 3. Most fungi were Isolated from Dendrobium sp. and Taxus sp.
文摘Objective：To investigate the anticancer activity of the total flavonoids isolated from a herbal formula,Xianhe Yanling Recipe（仙鹤延龄方）,a recipe commonly used in cancer patients in China.Methods： The in vitro anticancer activity of the total flavonoids was determined using the 3-（4,5-dimethylthiazole-2-yl）-2,5- diphenyltetrazolium bromide（MTT） assay on three cancer cell lines：MCF-7（a human breast adenocarcinoma cell line）,HepG-2（a human hepatocellular carcinoma cell line） and ES-2（a human ovarian cancer cell line）. The in vivo anticancer effect of the total flavonoids was assessed in a mouse tumor model bearing H22-induced hepatocellular carcinoma,and cisplatin was used as a positive control.Results：The total flavonoids exerted a powerful inhibitory effect on the three cell lines,with 50%inhibiting concentrations（IC_（50）） of 24.948,31.569 and 6.923μg/mL,respectively.In vivo studies showed that the total flavonoids had dose-dependent inhibitory effects on hepatocellular carcinoma in mice.Conclusion：The total flavonoids from Xianhe Yanling Recipe have potential anticancer activity,and further researches and development are warranted.
