This study was aimed to construct a biodegradable but reliable 3-β-hydroxybutymte biosensor. In this context a versatile paper based biosensor, quickly, easily and cheaply fabricated is reported. The procedure of fab...This study was aimed to construct a biodegradable but reliable 3-β-hydroxybutymte biosensor. In this context a versatile paper based biosensor, quickly, easily and cheaply fabricated is reported. The procedure of fabrication is based on the assumption that the introduction of the enzyme in the carbon ink will allow enzyme stabilization and facilitate the study of the catalysis of enzymes and the detection of substrates. To prove this concept we use the enzyme 3-hydroxybutyrate dehydrogenase, in aqueous solution. This enzyme was chosen because it catalyzes the 3-β-hydroxybutyrate, which results from ketoacidosis. The quantification this substance in the diabetics＇ blood is very important as it can increase the reliability of the diagnosis of glycaemia. To prove the multi-use of this biosensor we not only study the redox process in steady state and during the catalytic process, but also detected and quantify the 3-β-hydroxybutyrate. Our results showed that it was possible to study the redox process that occurred during the catalysis and to confirm the amino acid residues that participate in it. It was also observed that glucose and ascorbic acid can interfere in the detection and quantification of the 3-β-hydroxybutyrate, what should be in mind when the quantification of the 3-β-hydroxybutyrate is made in blood samples.展开更多
目的分析血清25-羟基维生素D_(3)[25-hydroxyvitamin D_(3),25-(OH)D_(3)]水平在IgA血管炎肾炎(immunoglobulin A vasculitis nephritis,IgAVN)患儿的临床病理特征及预后中的意义。方法回顾性分析2015年6月—2020年6月在安徽医科大学附...目的分析血清25-羟基维生素D_(3)[25-hydroxyvitamin D_(3),25-(OH)D_(3)]水平在IgA血管炎肾炎(immunoglobulin A vasculitis nephritis,IgAVN)患儿的临床病理特征及预后中的意义。方法回顾性分析2015年6月—2020年6月在安徽医科大学附属宿州医院和南京大学医学院附属金陵医院儿科完善肾活检的IgAVN患儿的临床资料。根据25-(OH)D_(3)水平将患儿分为正常组和低下组,收集并比较两组患儿的临床病理特征及随访资料。结果共纳入了359例IgAVN患儿,与正常组(62例)相比,低下组(297例)患儿便血比例、肉眼血尿比例、血清肌酐、血尿素氮、尿视黄醇蛋白、尿N-乙酰-β-D-氨基葡萄糖苷酶、尿蛋白定量、肾活检至尿蛋白转阴时间等指标均较高(P<0.05),估算的肾小球滤过率和白蛋白水平较低(P<0.05)。低下组肾脏病理显示肾小管间质损伤、新月体比例、肾小球节段硬化和肾间质炎症细胞浸润的发生比例高于正常组(P<0.05)。生存分析显示,低下组患儿的肾脏累积生存率较低(P<0.05)。多因素Cox回归分析显示血清25-(OH)D_(3)低下是IgAVN预后不良的独立危险因素(P<0.05)。结论血清25-(OH)D_(3)水平低下的IgAVN患儿的临床病理表现相对严重。血清25-(OH)D_(3)水平低下是儿童IgAVN预后不良的独立危险因素。展开更多
文摘This study was aimed to construct a biodegradable but reliable 3-β-hydroxybutymte biosensor. In this context a versatile paper based biosensor, quickly, easily and cheaply fabricated is reported. The procedure of fabrication is based on the assumption that the introduction of the enzyme in the carbon ink will allow enzyme stabilization and facilitate the study of the catalysis of enzymes and the detection of substrates. To prove this concept we use the enzyme 3-hydroxybutyrate dehydrogenase, in aqueous solution. This enzyme was chosen because it catalyzes the 3-β-hydroxybutyrate, which results from ketoacidosis. The quantification this substance in the diabetics＇ blood is very important as it can increase the reliability of the diagnosis of glycaemia. To prove the multi-use of this biosensor we not only study the redox process in steady state and during the catalytic process, but also detected and quantify the 3-β-hydroxybutyrate. Our results showed that it was possible to study the redox process that occurred during the catalysis and to confirm the amino acid residues that participate in it. It was also observed that glucose and ascorbic acid can interfere in the detection and quantification of the 3-β-hydroxybutyrate, what should be in mind when the quantification of the 3-β-hydroxybutyrate is made in blood samples.
文摘目的分析血清25-羟基维生素D_(3)[25-hydroxyvitamin D_(3),25-(OH)D_(3)]水平在IgA血管炎肾炎(immunoglobulin A vasculitis nephritis,IgAVN)患儿的临床病理特征及预后中的意义。方法回顾性分析2015年6月—2020年6月在安徽医科大学附属宿州医院和南京大学医学院附属金陵医院儿科完善肾活检的IgAVN患儿的临床资料。根据25-(OH)D_(3)水平将患儿分为正常组和低下组,收集并比较两组患儿的临床病理特征及随访资料。结果共纳入了359例IgAVN患儿,与正常组(62例)相比,低下组(297例)患儿便血比例、肉眼血尿比例、血清肌酐、血尿素氮、尿视黄醇蛋白、尿N-乙酰-β-D-氨基葡萄糖苷酶、尿蛋白定量、肾活检至尿蛋白转阴时间等指标均较高(P<0.05),估算的肾小球滤过率和白蛋白水平较低(P<0.05)。低下组肾脏病理显示肾小管间质损伤、新月体比例、肾小球节段硬化和肾间质炎症细胞浸润的发生比例高于正常组(P<0.05)。生存分析显示,低下组患儿的肾脏累积生存率较低(P<0.05)。多因素Cox回归分析显示血清25-(OH)D_(3)低下是IgAVN预后不良的独立危险因素(P<0.05)。结论血清25-(OH)D_(3)水平低下的IgAVN患儿的临床病理表现相对严重。血清25-(OH)D_(3)水平低下是儿童IgAVN预后不良的独立危险因素。
