A FISH procedure was adopted to physical mapping rice RFLP marker RZ69 and the BAC clone 38J9 screened by RZ69 which is linked to gene Bph3 in Oryza saliva L. and O. officinalis Well ex: Watt. The FISH results showed ...A FISH procedure was adopted to physical mapping rice RFLP marker RZ69 and the BAC clone 38J9 screened by RZ69 which is linked to gene Bph3 in Oryza saliva L. and O. officinalis Well ex: Watt. The FISH results showed that both 38J9 and RZ69 were located in the middle of 4S in O. officinalis and the centromere area of 4S in O. sativa. In O. officinalis the percentage distances from the centromere to the hybridization sites were 20.00 +/- 5.40 and 22.12 +/- 3.44, the detection rates were 50.00% and 6.14%, but in O. sativa they were 0 and 0, 56.10% and 6.29% correspondingly. The results obtained from the BAC and RFLP clone were almost the same in the cultivated rice and O. officinalis. It was suggested that die marker RZ69 of the cultivated rice and its homologous sequence in O. officinalis were in the same BAC clone, the homologous sequence of Bph3 in O. officinalis should be located at the sites hybridized by both RZ69 and 38J9. Many signals on different chromosomes of O. officinalis were observed under no blocking with Cot-1 DNA, showing that the repetitive sequences were also homologous between O. sativa and O. officinalis. The identification of chromosome 4 of O. officinalis is based on comparative map with RG214 and BAC clone screened by RG214. The feasibility of comparative physical mapping performed between O. sativa and O. officinalis with rice BAC clones was discussed.展开更多
The radiation shielding characteristics of 50wt% WO3/E44 epoxy composite in various gamma energies from 80 keV to 1.33 MeV are investigated via the MCNP code. Thus two scales are considered for WOa filler particles： ...The radiation shielding characteristics of 50wt% WO3/E44 epoxy composite in various gamma energies from 80 keV to 1.33 MeV are investigated via the MCNP code. Thus two scales are considered for WOa filler particles： micro and nano with sizes of i #m and 5Onto, respectively. The simulation results show that W03 nano particles exhibit a larger increase in linear attenuation coefficient in comparison with micro size particles. Finally, validation of simulation results with the published experimental data shows a good agreement.展开更多
Rosa sterilis S.D.Shi is an important economic tree in China that produces fruits with high nutritional and medicinal value.Many of R.sterills’organs are covered with different types of trichomes or prickles that dir...Rosa sterilis S.D.Shi is an important economic tree in China that produces fruits with high nutritional and medicinal value.Many of R.sterills’organs are covered with different types of trichomes or prickles that directly affect fruit appearance and plant management.This study used RNA sequencing technology to analyze the transcriptomes of two parts of the inflorescence branch,namely inflorescence stems with flagellated trichomes and pedicels with both flagellated and glandular trichomes.Comparative transcriptomic analysis showed that many transcription factors(TFs)are potentially involved in the formation and development of trichomes.The accumulation of RsETC1,a TF of the R3-MYB family,was significantly higher in inflorescence stems than in pedicels;quantitative reverse transcription PCR(qRTPCR)verified that its expression was significantly higher in inflorescence stems than in pedicels during the first three development stages,indicating its inhibitory action on the initiation of glandular trichomes in R.sterilis.The mRNA level of RsETC1 accumulated to significantly higher levels in trichomeless tissues than in tissues with trichromes,suggesting that this gene may inhibit the formation of trichomes in R.sterilis.Over-expression of RsETC1 in Arabidopsis resulted in glabrous phenotypes,and the expression of trichome-related endogenous genes,except for TTG1,was markedly reduced.In addition,the contents of the phytohormones jasmonic acid(JA),gibberellin A3(GA_(3)),and cytokinins(CKs)in pedicels were significantly higher than those in inflorescence stems,and the expression patterns of the genes related to hormone biosynthesis and signal transduction presented consistent responses,suggesting that the transduction of these hormones might be crucial for trichome initiation and development.These data provide a new perspective for revealing the molecular mechanism of trichome formation in R.sterilis.展开更多
The observation data collected by DSG3 precipitation phenomenon observation instrument and the manual parallel observation data in 5 stations of Gansu Province from January 2018 to December 2019 were compared and anal...The observation data collected by DSG3 precipitation phenomenon observation instrument and the manual parallel observation data in 5 stations of Gansu Province from January 2018 to December 2019 were compared and analyzed.The results show that the missing observation rate of the observation instrument was 0%,and the performance of the equipment was good;the empty forecast rate of drizzle was the highest;the false forecast rate of hail was the highest,and the accuracy of automatic observation records was relatively poor;the capture rate and empty forecast rate of rain and snow were higher,while their false forecast rate and missing forecast rate were lower.The capture rate and missing forecast rate of sleet were higher,and its missing forecast rate was low;the accuracy of automatic observation was better.It shows that the empty forecast and false forecast of DSG3 precipitation phenomenon observation instrument mainly occurred in weak precipitation phenomena,and the recognition of drizzle and hail was poor.展开更多
This scientific paper is a comparative analysis of two mathematical conjectures. The newly proposed -3(-n) - 1 Remer conjecture and how it is related to and a proof of the more well known 3n + 1 Collatz conjecture. An...This scientific paper is a comparative analysis of two mathematical conjectures. The newly proposed -3(-n) - 1 Remer conjecture and how it is related to and a proof of the more well known 3n + 1 Collatz conjecture. An overview of both conjectures and their respective iterative processes will be presented. Showcasing their unique properties and behavior to each other. Through a detailed comparison, we highlight the similarities and differences between these two conjectures and discuss their significance in the field of mathematics. And how they prove each other to be true.展开更多
Objective To evaluate and compare 3 kinds of cecocloln urinary reservoir. Methods Perm pouch, indiana pouch or detenial cecocolon reservoir has been performed for 37 patients. The outcome was evaluated by urodynamic a...Objective To evaluate and compare 3 kinds of cecocloln urinary reservoir. Methods Perm pouch, indiana pouch or detenial cecocolon reservoir has been performed for 37 patients. The outcome was evaluated by urodynamic and clinically. Results Good continence has been achieved in all patients with an appendiceal continence mechanism. The capacity and intrareservoir pressure of detenial cecocolon was significantly different from penn or indiana pouch 3 months postoperatively (P【0.01) whereas the difference became in no significant 12 months postoperativelly (P】0. 05). The intrareservoir pressure on contraction was lower with penn pouch. Conclusion The penn or modified indiana pouch is better and more ideal as a urinary reservoir whereas the detenial cecocolon pouch is technically simpler. 6 refs,1 tab.展开更多
A three-dimensional quantitative structure-activity relationship （3D-QSAR） study of a series of 7,8-dialkyl-l,3-diaminopyrrolo-[3,2-f] quinazolines with anticancer activity as dihydrofo- late reductase （DHFR） inhi...A three-dimensional quantitative structure-activity relationship （3D-QSAR） study of a series of 7,8-dialkyl-l,3-diaminopyrrolo-[3,2-f] quinazolines with anticancer activity as dihydrofo- late reductase （DHFR） inhibitors was carried out by using the comparative molecular field analysis （CoMFA）, on the basis of our reported 2D-QSAR of these compounds. The es- tablished 3D-QSAR model has good quality of statistics and good prediction ability; the non cross-validation correlation coefficient and the cross-validation value of this model are 0.993 and 0.619, respectively, the F value is 193.4, and the standard deviation SD is 0.208. This model indicates that the steric field factor plays a much more important role than the electrostatic one, in satisfying agreement with the published 2D-QSAR model. However, the 3D-QSAR model offers visual images of the steric field and the electrostatic field. The 3D-QSAR study further suggests the following： to improve the activity, the substituent R^1 should be selected to be a group with an adaptive bulk like Et or i-Pr, and the substituent R should be selected to be a larger alkyl. In particular, based on our present 3D-QSAR as well as the published 2D-QSAR, the experimentMly-proposed hydrophobic binding mechanism on the receptor-binding site of the DHFR can be further explained in theory. Therefore, the QSAR studies help to further understand the ＂hydrophobic binding＂ action mechanism of this kind of compounds, and to direct the molecular design of new drugs with higher activity.展开更多
应用比较分子力场分析(Comparative molecular force field analysis,CoMFA)方法研究了18种氟喹诺酮C-3噻唑酮衍生物对胰腺Capan-1细胞的体外抗增殖活性(p A).训练集中14个化合物用于建立预测模型,测试集6个化合物(含模板分子和新设计的...应用比较分子力场分析(Comparative molecular force field analysis,CoMFA)方法研究了18种氟喹诺酮C-3噻唑酮衍生物对胰腺Capan-1细胞的体外抗增殖活性(p A).训练集中14个化合物用于建立预测模型,测试集6个化合物(含模板分子和新设计的1个分子)作为模型验证.通过基于配体的原子契合的叠合方式,获得了训练集的统计显著模型.CoMFA模型使用3个主成分给出交叉验证系数(R 2 cv)值为0.436,非交叉验证系数(R 2)值为0.956,估计F值为72.217.结果显示,模型具有良好的稳健性与预测能力.基于CoMFA等高线图,揭示了该系列化合物抗增殖活性的一些关键结构因素.这些结果为理解其作用机制、设计具有高抗肿瘤活性的新型氟喹诺酮C-3噻唑酮类化合物提供有益的理论参考.展开更多
文摘A FISH procedure was adopted to physical mapping rice RFLP marker RZ69 and the BAC clone 38J9 screened by RZ69 which is linked to gene Bph3 in Oryza saliva L. and O. officinalis Well ex: Watt. The FISH results showed that both 38J9 and RZ69 were located in the middle of 4S in O. officinalis and the centromere area of 4S in O. sativa. In O. officinalis the percentage distances from the centromere to the hybridization sites were 20.00 +/- 5.40 and 22.12 +/- 3.44, the detection rates were 50.00% and 6.14%, but in O. sativa they were 0 and 0, 56.10% and 6.29% correspondingly. The results obtained from the BAC and RFLP clone were almost the same in the cultivated rice and O. officinalis. It was suggested that die marker RZ69 of the cultivated rice and its homologous sequence in O. officinalis were in the same BAC clone, the homologous sequence of Bph3 in O. officinalis should be located at the sites hybridized by both RZ69 and 38J9. Many signals on different chromosomes of O. officinalis were observed under no blocking with Cot-1 DNA, showing that the repetitive sequences were also homologous between O. sativa and O. officinalis. The identification of chromosome 4 of O. officinalis is based on comparative map with RG214 and BAC clone screened by RG214. The feasibility of comparative physical mapping performed between O. sativa and O. officinalis with rice BAC clones was discussed.
文摘The radiation shielding characteristics of 50wt% WO3/E44 epoxy composite in various gamma energies from 80 keV to 1.33 MeV are investigated via the MCNP code. Thus two scales are considered for WOa filler particles： micro and nano with sizes of i #m and 5Onto, respectively. The simulation results show that W03 nano particles exhibit a larger increase in linear attenuation coefficient in comparison with micro size particles. Finally, validation of simulation results with the published experimental data shows a good agreement.
基金supported by grants from the Joint Fund of the National Natural Science Foundation of China and the Karst Science Research Center of Guizhou Province,China(U1812401)the Talent Project of Guizhou Province,China(20164016)。
文摘Rosa sterilis S.D.Shi is an important economic tree in China that produces fruits with high nutritional and medicinal value.Many of R.sterills’organs are covered with different types of trichomes or prickles that directly affect fruit appearance and plant management.This study used RNA sequencing technology to analyze the transcriptomes of two parts of the inflorescence branch,namely inflorescence stems with flagellated trichomes and pedicels with both flagellated and glandular trichomes.Comparative transcriptomic analysis showed that many transcription factors(TFs)are potentially involved in the formation and development of trichomes.The accumulation of RsETC1,a TF of the R3-MYB family,was significantly higher in inflorescence stems than in pedicels;quantitative reverse transcription PCR(qRTPCR)verified that its expression was significantly higher in inflorescence stems than in pedicels during the first three development stages,indicating its inhibitory action on the initiation of glandular trichomes in R.sterilis.The mRNA level of RsETC1 accumulated to significantly higher levels in trichomeless tissues than in tissues with trichromes,suggesting that this gene may inhibit the formation of trichomes in R.sterilis.Over-expression of RsETC1 in Arabidopsis resulted in glabrous phenotypes,and the expression of trichome-related endogenous genes,except for TTG1,was markedly reduced.In addition,the contents of the phytohormones jasmonic acid(JA),gibberellin A3(GA_(3)),and cytokinins(CKs)in pedicels were significantly higher than those in inflorescence stems,and the expression patterns of the genes related to hormone biosynthesis and signal transduction presented consistent responses,suggesting that the transduction of these hormones might be crucial for trichome initiation and development.These data provide a new perspective for revealing the molecular mechanism of trichome formation in R.sterilis.
文摘The observation data collected by DSG3 precipitation phenomenon observation instrument and the manual parallel observation data in 5 stations of Gansu Province from January 2018 to December 2019 were compared and analyzed.The results show that the missing observation rate of the observation instrument was 0%,and the performance of the equipment was good;the empty forecast rate of drizzle was the highest;the false forecast rate of hail was the highest,and the accuracy of automatic observation records was relatively poor;the capture rate and empty forecast rate of rain and snow were higher,while their false forecast rate and missing forecast rate were lower.The capture rate and missing forecast rate of sleet were higher,and its missing forecast rate was low;the accuracy of automatic observation was better.It shows that the empty forecast and false forecast of DSG3 precipitation phenomenon observation instrument mainly occurred in weak precipitation phenomena,and the recognition of drizzle and hail was poor.
文摘This scientific paper is a comparative analysis of two mathematical conjectures. The newly proposed -3(-n) - 1 Remer conjecture and how it is related to and a proof of the more well known 3n + 1 Collatz conjecture. An overview of both conjectures and their respective iterative processes will be presented. Showcasing their unique properties and behavior to each other. Through a detailed comparison, we highlight the similarities and differences between these two conjectures and discuss their significance in the field of mathematics. And how they prove each other to be true.
文摘Objective To evaluate and compare 3 kinds of cecocloln urinary reservoir. Methods Perm pouch, indiana pouch or detenial cecocolon reservoir has been performed for 37 patients. The outcome was evaluated by urodynamic and clinically. Results Good continence has been achieved in all patients with an appendiceal continence mechanism. The capacity and intrareservoir pressure of detenial cecocolon was significantly different from penn or indiana pouch 3 months postoperatively (P【0.01) whereas the difference became in no significant 12 months postoperativelly (P】0. 05). The intrareservoir pressure on contraction was lower with penn pouch. Conclusion The penn or modified indiana pouch is better and more ideal as a urinary reservoir whereas the detenial cecocolon pouch is technically simpler. 6 refs,1 tab.
基金This work was supported by the National Natural Science Foundation of China （No.20673148）. We also heartily thank the College of Life Sciences, Sun Yat-Sen University for the SYBYL 6.9 computation environment support.
文摘A three-dimensional quantitative structure-activity relationship （3D-QSAR） study of a series of 7,8-dialkyl-l,3-diaminopyrrolo-[3,2-f] quinazolines with anticancer activity as dihydrofo- late reductase （DHFR） inhibitors was carried out by using the comparative molecular field analysis （CoMFA）, on the basis of our reported 2D-QSAR of these compounds. The es- tablished 3D-QSAR model has good quality of statistics and good prediction ability; the non cross-validation correlation coefficient and the cross-validation value of this model are 0.993 and 0.619, respectively, the F value is 193.4, and the standard deviation SD is 0.208. This model indicates that the steric field factor plays a much more important role than the electrostatic one, in satisfying agreement with the published 2D-QSAR model. However, the 3D-QSAR model offers visual images of the steric field and the electrostatic field. The 3D-QSAR study further suggests the following： to improve the activity, the substituent R^1 should be selected to be a group with an adaptive bulk like Et or i-Pr, and the substituent R should be selected to be a larger alkyl. In particular, based on our present 3D-QSAR as well as the published 2D-QSAR, the experimentMly-proposed hydrophobic binding mechanism on the receptor-binding site of the DHFR can be further explained in theory. Therefore, the QSAR studies help to further understand the ＂hydrophobic binding＂ action mechanism of this kind of compounds, and to direct the molecular design of new drugs with higher activity.
文摘应用比较分子力场分析(Comparative molecular force field analysis,CoMFA)方法研究了18种氟喹诺酮C-3噻唑酮衍生物对胰腺Capan-1细胞的体外抗增殖活性(p A).训练集中14个化合物用于建立预测模型,测试集6个化合物(含模板分子和新设计的1个分子)作为模型验证.通过基于配体的原子契合的叠合方式,获得了训练集的统计显著模型.CoMFA模型使用3个主成分给出交叉验证系数(R 2 cv)值为0.436,非交叉验证系数(R 2)值为0.956,估计F值为72.217.结果显示,模型具有良好的稳健性与预测能力.基于CoMFA等高线图,揭示了该系列化合物抗增殖活性的一些关键结构因素.这些结果为理解其作用机制、设计具有高抗肿瘤活性的新型氟喹诺酮C-3噻唑酮类化合物提供有益的理论参考.
