A generalized upwind scheme with fractional steps for 3-D mathematical models of convection dominating groundwater quality is suggested. The mass transport equation is split into a convection equation and a dispersive...A generalized upwind scheme with fractional steps for 3-D mathematical models of convection dominating groundwater quality is suggested. The mass transport equation is split into a convection equation and a dispersive equation. The generalized upwind scheme is used to solve the convection equation and the finite element method is used to compute the dispersive equation.These procedures which not only overcome the phenomenon of the negative concentration and numerical dispersion appear frequently with normal FEM or FDM to solve models of convection dominating groundwater transport but also avoid the step for computing each node velocity give a more suitable method to calculate the concentrations of the well points.展开更多
[Objective] The paper was to develop a subunit vaccine candidate for prevention and control of goose parvovirus infection. [Method]Based on the prokaryotic expression system, the antigenic epitopes and locations of th...[Objective] The paper was to develop a subunit vaccine candidate for prevention and control of goose parvovirus infection. [Method]Based on the prokaryotic expression system, the antigenic epitopes and locations of the structural protein VP3 were predicted by software analysis,and the region displaying a large portion of antigenic epitopes was amplified by PCR. The target VP3 DNA fragment was inserted into pET-30 a-VP3 vector, was transformed into Escherichia coli BL21 competent cells for protein expression and animal test. The SPF chickens were immunized with the recombinant protein and the antisera were collected for neutralization test by using a goose embryo fibroblast. [Result] The recombinant plasmid was constructed, and the target region of VP3 protein was expressed efficiently in a soluble form. The neutralizing titers of antisera could reach up to-2.608. [Conclusion] The target region displaying a large portion of antigenic epitopes of the structural protein VP3 could be expressed efficiently in soluble form, and the expressed protein could induce neutralizing antibodies in SFP chicken.展开更多
Similar reservoir sandbodies and fault conduit systems in the sandstone reservoirs in the middle Es3 member of the Niuzhuang Sag have been problematic for a long time. The following problems remain unsolved： 1） The ...Similar reservoir sandbodies and fault conduit systems in the sandstone reservoirs in the middle Es3 member of the Niuzhuang Sag have been problematic for a long time. The following problems remain unsolved： 1） The distribution of sandstone porosity is inconsistent with the hydrocarbon accumulation. The oil sandstones have low porosity instead of high porosity. 2） Sandstones, which have the same properties, have different levels of oiliness, and the sandstones with almost the same properties show different degrees of oil-bearing capacity. This study analyzes the condition of reservoirs in the research area during the accumulation period in terms of paleoporosity estimation and discusses the critical porosity of the sandstone reservoirs during the same period. The following conclusions can be drawn from the results. 1） Although reservoir properties are low at present and some reservoirs have become tight, the paleoporosity ranging from 18% to 25% is greater than the critical porosity of 13.9%. As the： loss of porosity is different in terms of burial history, the present porosity cannot reflect porosity during the accumulation period. Similar/y, high porosity during the accumulation period does not indicate that tbe present porosity is high. 2） The present reservoir location is consistent with the distribution of high paleoporosity during the accumulation period. This result indicates that high porosity belts are prone to hydrocarbon accumulation because of the dominant migration pathways generated as a result of property discrepancies under similar fault conduit conditions. Consequently, the hydrocarbon mainly accumulates in high porosity belts. Paleoporosity during the accumulation period is found to be a vital controlling factor. Therefore, high paleoporosity sandstones in the middle Es3 member of the Niuzhuang Sag have great potential for future exploration.展开更多
Background:The etiology of pulmonary arterial hypertension associated with congenital heart disease(PAHCHD)is complicated and the phenotype is heterogeneous.Genetic defects of NOTCH3 were associated withcerebral disea...Background:The etiology of pulmonary arterial hypertension associated with congenital heart disease(PAHCHD)is complicated and the phenotype is heterogeneous.Genetic defects of NOTCH3 were associated withcerebral disease and pulmonary hypertension.However,the relationship between NOTCH3 mutations and theclinical phenotype has not been reported in CHD-PAH.Methods:We eventually enrolled 142 PAH-CHD patientsfrom Fuwai Hospital.Whole exome sequencing(WES)was performed to screen the rare deleterious variants ofNOTCH3 gene.Results:This PAH-CHD cohort included 43(30.3%)men and 99(69.7%)women with the meanage 29.8±10.9 years old.The pathogenic or likely pathogenic mutations of NOTCH3 were identified in five cases.Patients 2,5,8 and 11 carried the same NOTCH3 mutation c.1630C>T(pArg544Cys),which is the hot-spotmutation for cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy(CADASIL).Patient 3 carried the NOTCH3 mutation p.Arg75Gln that has also been reported to be associatedwith the CADASIL.Patients 2,5,8,11 took the examination of the cerebral magnetic resonance imaging(MRI)and confirmed the phenotype of CADASIL.Conclusions:We first reported the NOTCH3 rare mutationsand CADASIL phenotypes in CHD-PAH patients.The NOTCH3 rare variants were with a relatively high positiverate and CADASIL phenotypes were likely enriched in PAH-CHD patients.The preoperative neurological examinationmight be recommended for PAH-CHD patients to determine the surgical contraindications and reduceintraoperative neurological complications.展开更多
文摘A generalized upwind scheme with fractional steps for 3-D mathematical models of convection dominating groundwater quality is suggested. The mass transport equation is split into a convection equation and a dispersive equation. The generalized upwind scheme is used to solve the convection equation and the finite element method is used to compute the dispersive equation.These procedures which not only overcome the phenomenon of the negative concentration and numerical dispersion appear frequently with normal FEM or FDM to solve models of convection dominating groundwater transport but also avoid the step for computing each node velocity give a more suitable method to calculate the concentrations of the well points.
基金Supported by Youth Fund Project of Natural Science Foundation of Shandong Province(ZR2014CQ009)Science and Technology Development Program of Binzhou City(2013GG0304)
文摘[Objective] The paper was to develop a subunit vaccine candidate for prevention and control of goose parvovirus infection. [Method]Based on the prokaryotic expression system, the antigenic epitopes and locations of the structural protein VP3 were predicted by software analysis,and the region displaying a large portion of antigenic epitopes was amplified by PCR. The target VP3 DNA fragment was inserted into pET-30 a-VP3 vector, was transformed into Escherichia coli BL21 competent cells for protein expression and animal test. The SPF chickens were immunized with the recombinant protein and the antisera were collected for neutralization test by using a goose embryo fibroblast. [Result] The recombinant plasmid was constructed, and the target region of VP3 protein was expressed efficiently in a soluble form. The neutralizing titers of antisera could reach up to-2.608. [Conclusion] The target region displaying a large portion of antigenic epitopes of the structural protein VP3 could be expressed efficiently in soluble form, and the expressed protein could induce neutralizing antibodies in SFP chicken.
基金supported by the Young Scholars Development Fund of SWPU
文摘Similar reservoir sandbodies and fault conduit systems in the sandstone reservoirs in the middle Es3 member of the Niuzhuang Sag have been problematic for a long time. The following problems remain unsolved： 1） The distribution of sandstone porosity is inconsistent with the hydrocarbon accumulation. The oil sandstones have low porosity instead of high porosity. 2） Sandstones, which have the same properties, have different levels of oiliness, and the sandstones with almost the same properties show different degrees of oil-bearing capacity. This study analyzes the condition of reservoirs in the research area during the accumulation period in terms of paleoporosity estimation and discusses the critical porosity of the sandstone reservoirs during the same period. The following conclusions can be drawn from the results. 1） Although reservoir properties are low at present and some reservoirs have become tight, the paleoporosity ranging from 18% to 25% is greater than the critical porosity of 13.9%. As the： loss of porosity is different in terms of burial history, the present porosity cannot reflect porosity during the accumulation period. Similar/y, high porosity during the accumulation period does not indicate that tbe present porosity is high. 2） The present reservoir location is consistent with the distribution of high paleoporosity during the accumulation period. This result indicates that high porosity belts are prone to hydrocarbon accumulation because of the dominant migration pathways generated as a result of property discrepancies under similar fault conduit conditions. Consequently, the hydrocarbon mainly accumulates in high porosity belts. Paleoporosity during the accumulation period is found to be a vital controlling factor. Therefore, high paleoporosity sandstones in the middle Es3 member of the Niuzhuang Sag have great potential for future exploration.
基金Grant 81425002 from the National Science Fund for Distinguished Young ScholarsGrants 81670052,and 81870050 from the National Natural Science Foundation of ChinaGrant 2018ZX09711001-003-012 from the Drug Innovation Major Project,CAMS Fund for Key Laboratory of Pulmonary Vascular Medicine(2017PT32016).
文摘Background:The etiology of pulmonary arterial hypertension associated with congenital heart disease(PAHCHD)is complicated and the phenotype is heterogeneous.Genetic defects of NOTCH3 were associated withcerebral disease and pulmonary hypertension.However,the relationship between NOTCH3 mutations and theclinical phenotype has not been reported in CHD-PAH.Methods:We eventually enrolled 142 PAH-CHD patientsfrom Fuwai Hospital.Whole exome sequencing(WES)was performed to screen the rare deleterious variants ofNOTCH3 gene.Results:This PAH-CHD cohort included 43(30.3%)men and 99(69.7%)women with the meanage 29.8±10.9 years old.The pathogenic or likely pathogenic mutations of NOTCH3 were identified in five cases.Patients 2,5,8 and 11 carried the same NOTCH3 mutation c.1630C>T(pArg544Cys),which is the hot-spotmutation for cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy(CADASIL).Patient 3 carried the NOTCH3 mutation p.Arg75Gln that has also been reported to be associatedwith the CADASIL.Patients 2,5,8,11 took the examination of the cerebral magnetic resonance imaging(MRI)and confirmed the phenotype of CADASIL.Conclusions:We first reported the NOTCH3 rare mutationsand CADASIL phenotypes in CHD-PAH patients.The NOTCH3 rare variants were with a relatively high positiverate and CADASIL phenotypes were likely enriched in PAH-CHD patients.The preoperative neurological examinationmight be recommended for PAH-CHD patients to determine the surgical contraindications and reduceintraoperative neurological complications.
