GAS6-AS1调节miR-370-3p/SPATA2轴对卵巢癌细胞增殖、迁移、侵袭、凋亡和EMT的影响

目的:探讨长链非编码RNA GAS6反义RNA1(long non-coding RNA GAS6 antisense RNA1, lncRNA GAS6-AS1)调节miR-370-3p/精子发生相关蛋白2 (spermatogenesis-associated protein 2, SPATA2)轴对卵巢癌细胞增殖、迁移、侵袭、凋亡和上皮间质转化(epithelial mesenchymal transformation, EMT)的影响。方法:qRT-PCR、Western blot分别检测癌旁组织、卵巢癌组织、人正常卵巢上皮细胞IOSE80及卵巢癌细胞系HO-8910、SKOV3、A2780中GAS6-AS1、miR-370-3p及SPATA2蛋白表达。将SKOV3细胞分为:对照组(NC组)、 si-NC组、si-GAS6-AS1组、mimic NC组、miR-370-3p mimic组、si-GAS6-AS1+inhibitor NC组、si-GAS6-AS1+miR-370-3p inhibitor组,qRT-PCR检测细胞中GAS6-AS1、miR-370-3p表达;CCK-8法检测细胞增殖;流式细胞术检测细胞凋亡;划痕愈合实验检测细胞迁移;Transwell实验检测细胞侵袭;Western blot检测SPATA2、细胞周期素D1(CyclinD1)、Bcl-2相关X蛋白(Bcl-2-associated X,Bax)、E-钙黏蛋白(E-cadherin)、波形蛋白(Vimentin)、神经钙黏蛋白(N-cadherin)表达;双荧光素酶报告基因实验检测GAS6-AS1与miR-370-3p、 miR-370-3p与SPATA2的关系。结果:在卵巢癌组织和细胞中GAS6-AS1、SPATA2蛋白高表达,miR-370-3p低表达,且在SKOV3细胞中GAS6-AS1、SPATA2蛋白表达量最高,miR-370-3p表达水平最低,因此,选择SKOV3细胞为后续研究对象。与NC组、si-NC组比较,si-GAS6-AS1组GAS6-AS1、OD450值(24 h、48 h、72 h)、划痕愈合率、侵袭细胞数、SPATA2、CyclinD1、Vimentin、N-cadherin蛋白表达降低,miR-370-3p表达、细胞凋亡率、Bax、E-cadherin蛋白表达升高(P<0.05);与NC组、mimic NC组比较,miR-370-3p mimic组OD450值(24 h、48 h、72 h)、划痕愈合率、侵袭细胞数、SPATA2、CyclinD1、Vimentin、N-cadherin蛋白表达降低,miR-370-3p表达、细胞凋亡率、Bax、E-cadherin蛋白表达升高(P<0.05);miR-370-3p inhibitor减弱了沉默GAS6-AS1对SKOV3细胞增殖、迁移、侵袭及EMT的抑制及对细胞凋亡的促进作用。GAS6-AS1与miR-370-3p、miR-370-3p与SPATA2存在靶向调控关系。结论:沉默GAS6-AS1通过上调miR-370-3p来抑制SPATA2表达,从而抑制SKOV3细胞增殖、迁移、侵袭及EMT,并促进细胞凋亡。

3D and 2D topographic correction to estimated geothermal gradient from the base of gas hydrate stability zone in the Andaman Forearc Basin

Methane gas hydrate related bottom-simulating reflectors(BSRs)are imaged based on the in-line and cross-line multi-channel seismic(MCS)data from the Andaman Forearc Basin.The depth of the BSR depends on pressure and temperature and pore water salinity.With these assumptions,the BSR depth can be used to estimate the geothermal gradient(GTG)based on the availability of in-situ temperature measurements.This calculation is done assuming a 1D conductive model based on available in-situ temperature measurement at site NGHP-01-17 in the study area.However,in the presence of seafloor topography,the conductive temperature field in the subsurface is affected by lateral refraction of heat,which focuses heat in topographic lows and away from topographic highs.The 1D estimate of GTG in the Andaman Forearc Basin has been validated by drilling results from the NGHP-01 expedition.2D analytic modeling to estimate the effects of topography is performed earlier along selected seismic profiles in the study area.The study extended to estimate the effect of topography in 3D using a numerical model.The corrected GTG data allow us to determine GTG values free of topographic effect.The difference between the estimated GTG and values corrected for the 3D topographic effect varies up to~5℃/km.These conclude that the topographic correction is relatively small compared to other uncertainties in the 1D model and that apparent GTG determined with the 1D model captures the major features,although the correction is needed prior to interpreting subtle features of the derived GTG maps.

GAS7、ALDH3B1及AHNAK在急性髓系白血病治疗前后的表达变化及机制研究

探索在急性髓系白血病(AML)治疗前及缓解后GAS7、ALDH3B1及AHNAK基因的表达变化及机制研究。方法:通过使用实时荧光定量 RT-PCR方法检测GAS7、ALDH3B1及AHNAK基因在33例初诊急性髓系白血病(AML)患者治疗前及完全缓解后骨髓中GAS7、ALDH3B1及AHNAK基因的表达情况。结果:GAS7、ALDH3B1及AHNAK在初诊急性髓系白血病(AML)患者骨髓中相对表达量低于治疗后缓解组中的表达水平 ( P<0.05)。结论: GAS7、ALDH3B1及AHNAK基因在急性髓系白血病中的表达水平较缓解后表达水平低,考虑GAS7、ALDH3B1及AHNAK基因可能参与了急性髓系白血病的发生发展。

Deep-large faults controlling on the distribution of the venting gas hydrate system in the middle of the Qiongdongnan Basin, South China Sea

Many locations with concentrated hydrates at vents have confirmed the presence of abundant thermogenic gas in the middle of the Qiongdongnan Basin(QDNB).However,the impact of deep structures on gasbearing fluids migration and gas hydrates distribution in tectonically inactive regions is still unclear.In this study,the authors apply high-resolution 3D seismic and logging while drilling(LWD)data from the middle of the QDNB to investigate the influence of deep-large faults on gas chimneys and preferred gasescape pipes.The findings reveal the following:(1)Two significant deep-large faults,F1 and F2,developed on the edge of the Songnan Low Uplift,control the dominant migration of thermogenic hydrocarbons and determine the initial locations of gas chimneys.(2)The formation of gas chimneys is likely related to fault activation and reactivation.Gas chimney 1 is primarily arises from convergent fluid migration resulting from the intersection of the two faults,while the gas chimney 2 benefits from a steeper fault plane and shorter migration distance of fault F2.(3)Most gas-escape pipes are situated near the apex of the two faults.Their reactivations facilitate free gas flow into the GHSZ and contribute to the formation of fracture‐filling hydrates.

Coking of Pt/γ-Al_(2)O_(3) catalyst in landfill gas deoxygen and its effects on catalytic performance

Catalytic oxidation of CH_(4) has been proved to be an attractive option for landfill gas(LFG) upgrading.However, coking of catalysts in catalytic LFG deoxygen has been clearly observed in industrial applications. In this regard, it is necessary to investigate whether coke deposition originates from CH_(4) or volatile organic compounds present in LFG, and the influence of coke deposition on catalytic performance. Herein,we evaluate the LFG deoxygen on Pt/γ-Al_(2)O_(3) catalyst in simulated LFG(CH_(4), CO_(2), O_(2), N_(2)) and its co-feed with representative volatile organic compounds, ethylbenzene, toluene, benzene and cyclohexane. The results show that the coking of the catalyst is originated from volatile organic compounds rather than CH_(4). The Pt/γ-Al_(2)O_(3) catalyst does not deactivate during LFG deoxygen process, even significant amount of coke deposited, up to 18.15%(mass). Characterization analyses reveal that although coke deposition overall covers the catalyst surface, resulting in mesopores blockage and a reduced number of accessible Pt sites, however, the coke formed, H-rich carbonaceous components, behaves as counterpart for O_(2) elimination. Besides, the coke deposited is mainly filamentous. Thus, coke formation has little negative effect on the overall catalytic performance of Pt/γ-Al_(2)O_(3) catalyst ultimately. The results obtained in this work are helpful for the rational design of robust Pt based catalysts for LFG deoxygen without undue attention to their coking properties, and also favor the innovation of more attractive purification scheme configurations.

Elimination of cracks in stainless steel casings via 3D printed sand molds with an internal topology structure

The important supporting component in a gas turbine is the casing,which has the characteristics of large size,complex structure,and thin wall.In the context of existing 3DP sand casting processes,casting crack defects are prone to occur.This leads to an increase in the scrap rate of casings,causing significant resource wastage.Additionally,the presence of cracks poses a significant safety hazard after the casings are put into service.The generation of different types of crack defects in stainless steel casings is closely related to casting stress and the high-temperature concession of the sand mold.Therefore,the types and causes of cracks in stainless steel casing products,based on their structural characteristics,were systematically analyzed.Various sand molds with different internal topology designs were printed using the 3DP technology to investigate the impact of sand mold structures on high-temperature concession.The optimal sand mold structure was used to cast casings,and the crack suppression effect was verified by analyzing its eddy current testing results.The experimental results indicate that the skeleton structure has an excellent effect on suppressing cracks in the casing.This research holds important theoretical and engineering significance in improving the quality of casing castings and reducing production costs.

Prediction and quantification of effective gas source rocks in a lacustrine basin:Western Depression in the Liaohe Subbasin,China

Due to limited data on the geochemical properties of natural gas,estimations are needed for the effective gas source rock in evaluating gas potential.However,the pronounced heterogeneity of mudstones in lacustrine successions complicates the prediction of the presence and geochemical characteristics of gas source rocks.In this paper,the Liaohe Subbasin of Northeast China is used as an example to construct a practical methodology for locating effective gas source rocks in typical lacustrine basins.Three types of gas source rocks,microbial,oil-type,and coal-type,were distinguished according to the different genetic types of their natural gas.A practical three-dimensional geological model was developed,refined,and applied to determine the spatial distribution of the mudstones in the Western Depression of the Liaohe Subbasin and to describe the geochemical characteristics(the abundance,type,and maturation levels of the organic matter).Application of the model in the subbasin indicates that the sedimentary facies have led to heterogeneity in the mudstones,particularly with respect to organic matter types.The effective gas source rock model constructed for the Western Depression shows that the upper sequence(SQ2)of the Fourth member(Mbr 4)of the Eocene Shahejie Formation(Fm)and the lower and middle sequences(SQ3 and SQ4)of the Third member(Mbr 3)form the principal gas-generating interval.The total volume of effective gas source rocks is estimated to be 586 km^(3).The effective microbial,oil-type,and coal-type gas source rocks are primarily found in the shallow western slope,the central sags,and the eastern slope of the Western Depression,respectively.This study provides a practical approach for more accurately identifying the occurrence and geochemical characteristics of effective natural gas source rocks,enabling a precise quantitative estimation of natural gas reserves.

The Al_(2)O_(3)and Mn/Al_(2)O_(3)sorbents highly utilized in destructive sorption of NF_(3)

NF_(3)is commonly used as an etching and cleaning gas in semiconductor industry,however it is a strongly greenhouse gas.Therefore,the destruction of disposal NF_(3)is an urgent task to migrate the greenhouse effect.Among the technologies for NF_(3)abatement,the destructive sorption of NF_(3)over metal oxides sorbents is an effective way.Thus,the search for a highly reactive and utilized sorbent for NF_(3)destruction is in great demand.In this work,AlOOH supported on carbon-sphere(AlOOH/CS)as precursors were synthesized hydrothermally and heat-treated to prepare the Al_(2)O_(3)sorbents.The influence of AlOOH/CS hydrothermal temperatures on the reactivity of derived Al_(2)O_(3)sorbents for NF_(3)destruction was investigated,and it is shown that the Al2O3 from AlOOH/CS hydro-thermalized at 120℃is superior to others.Subsequently,the optimized Al_(2)O_(3)was covered by Mn(OH)x to prepare Mn/Al_(2)O_(3)sorbents via changing hydrothermal temperatures and Mn loadings.The results show that the Mn/Al_(2)O_(3)sorbents are more utilized than bare Al_(2)O_(3)in NF_(3)destructive sorption due to the promotional effect of Mn_(2)O_(3)as surface layer on the fluorination of Al_(2)O_(3)as substrate,especially the optimal 5%Mn/Al2O3(160℃)exhibits a utilization percentage as high as 90.4%,and remarkably exceeds all the sorbents reported so far.These findings are beneficial to develop more efficient sorbents for the destruction of NF_(3).

Comparison of Perovskite Systems Based on AFeO_(3)(A=Ce,La,Y)in CO_(2) Hydrogenation to CO

CO_(2) is the most cost-eff ective and abundant carbon resource,while the reverse water-gas reaction(rWGS)is one of the most eff ective methods of CO_(2) utilization.This work presents a comparative study of rWGS activity for perovskite systems based on AFeO_(3)(where A=Ce,La,Y).These systems were synthesized by solution combustion synthesis(SCS)with diff erent ratios of fuel(glycine)and oxidizer(φ),diff erent amounts of NH 4 NO_(3),and the addition of alumina or silica as supports.Various techniques,including X-ray diff raction analysis,thermogravimetric analysis,Fourier transform infrared spectroscopy(FTIR),scanning electron microscopy,energy-dispersive X-ray spectroscopy,N 2-physisorption,H_(2) temper-ature-programmed reduction,temperature-programmed desorption of H_(2) and CO_(2),Raman spectroscopy,and in situ FTIR,were used to relate the physicochemical properties with the catalytic performance of the obtained composites.Each specifi c perovskite-containing system(either bulk or supported)has its own optimalφand NH_(4) NO_(3) amount to achieve the highest yield and dispersion of the perovskite phase.Among all synthesized systems,bulk SCS-derived La-Fe-O systems showed the highest resistance to reducing environments and the easiest hydrogen desorption,outperforming La-Fe-O produced by solgel combustion(SGC).CO_(2) conversion into CO at 600°C for bulk ferrite systems,depending on the A-cation type and preparation method,follows the order La(SGC)<Y<Ce<La(SCS).The diff erences in properties between La-Fe-O obtained by the SCS and SGC methods can be attributed to diff erent ratios of oxygen and lanthanum vacancy contributions,hydroxyl coverage,morphology,and free iron oxide presence.In situ FTIR data revealed that CO_(2) hydrogenation occurs through formates generated under reaction conditions on the bulk system based on La-Fe-O,obtained by the SCS method.γ-Al_(2)O_(3) improves the dispersion of CeFeO_(3) and LaFeO_(3) phases,the specifi c surface area,and the quantity of adsorbed H_(2) and CO_(2).This led to a signifi cant increase in CO_(2) conversion for supported CeFeO_(3) but not for the La-based system compared to bulk and SiO_(2)-supported perovskite catalysts.However,adding alumina increased the activity per mass for both Ce-and La-based perovskite systems,reducing the amount of rare-earth components in the catalyst and thereby lowering the cost without substantially compromising stability.

Heteroatoms doped iron oxide-based catalyst prepared from zinc slag for efficient selective catalytic reduction of NO_(x) with NH_(3)

Excessive emissions of nitrogen oxides from flue gas have imposed various detrimental impacts on environment,and the development of deNO_(x) catalysts with low-cost and high performance is an urgent requirement.Iron oxide-based material has been explored for promising deNO_(x) catalysts.However,the unsatisfactory low-temperature activity limits their practical applications.In this study,a series of excellent low-temperature denitrification catalysts(Ha-FeO_(x)/yZS)were prepared by acid treatment of zinc slag,and the mass ratios of Fe to impure ions was regulated by adjusting the acid concentrations.Ha-FeO_(x)/yZS showed high denitrification performance(>90%)in the range of 180–300℃,and the optimal NO conversion and N2 selectivity were higher than 95%at 250℃.Among them,the Ha-FeO_(x)/2ZS synthesized with 2 mol/L HNO3 exhibited the widest temperature window(175–350℃).The excellent denitrification performance of Ha-FeO_(x)/yZS was mainly attributed to the strong interaction between Fe and impurity ions to inhibit the growth of crystals,making Ha-FeO_(x)/yZS with amorphous structure,nice fine particles,large specific surface area,more surface acid sites and high chemisorbed oxygen.The in-situ DRIFT experiments confirmed that the SCR reaction on the Ha-FeO_(x)/yZS followed both Langmuir-Hinshelwood(L-H)mechanism and Eley-Rideal(E-R)mechanism.The present work proposed a high value-added method for the preparation of cost-effective catalysts from zinc slag,which showed a promising application prospect in NO_(x) removal by selective catalytic reduction with ammonia.

Research and development of 3PP coating steel pipes

The industrial application of an exterior three-layer anticorrosive polypropylene coating system(3PP)on large-diameter(larger than Φ600 mm)steel pipes was developed using an experimental process simulation study and the optimization of raw materials inspection,steel pipe surface pretreatments,and water cooling control on a coating application process.The coating properties meet ISO standard 21809 on buried or submerged 3PP pipelines used in the petroleum and natural gas industries.Differential scanning calorimetry and X-ray diffraction were used to analyze the crystallinities and grain sizes of polypropylene(PP)top coats with different cooling rates.Increasing the melt cooling rate reduces the crystallinity and grain size of the PP top coat and enhances its strength and toughness.

Integrated application of 3D seismic and microseismic data in the development of tight gas reservoirs

The development of unconventional resources, such as shale gas and tight sane gas, requires the integration of multi-disciplinary knowledge to resolve many engineering problems in order to achieve economic production levels. The reservoir heterogeneit3 revealed by different data sets, such as 3D seismic and microseismic data, can more full3 reflect the reservoir properties and is helpful to optimize the drilling and completioT programs. First, we predict the local stress direction and open or close status of the natura fractures in tight sand reservoirs based on seismic curvature, an attribute that reveals reservoi heterogeneity and geomechanical properties. Meanwhile, the reservoir fracture network is predicted using an ant-tracking cube and the potential fracture barriers which can affec hydraulic fracture propagation are predicted by integrating the seismic curvature attribute anc ant-tracking cube. Second, we use this information, derived from 3D seismic data, to assis in designing the fracture program and adjusting stimulation parameters. Finally, we interpre the reason why sand plugs will occur during the stimulation process by the integration of 3E seismic interpretation and microseismic imaging results, which further explain the hydraulic fracure propagation controlling factors and open or closed state of natural fractures in tigh sand reservoirs.

Hierarchical Radial Co3O4 Microcrystal and Application in Gas Sensor

Three-dimensional （3D） hierarchical Co3O4 microcrystal with radial dendritic morphologies was prepared through hydrothermal reactions followed by subsequent annealing treatment. Structural and morphological characterizations were performed by X-ray diffraction, scan-ning electron microscopy and transmission electron microscopy. The gas sensing properties of the as-obtained microcrystal were investigated at 110 oC, which revealed that the 3D hierarchical porous Co3O4 microcrystal exhibited high sensitivity to ammonia, as well as a short response time of 10 s. The response characteristic indicates that the sensor has a good stability and reversibility. Detections of toxic and flammable gases, such as ethanol, acetone and benzene were also carried out at a relative low temperature. The results indicate that such hierarchical Co3O4 microcrystal would be a potential material in the field of gas sensing.

Effects of Superphosphate Addition on NH_3 and Greenhouse Gas Emissions During Vegetable Waste Composting

To study the effects of superphosphate（SP） on the NH_3 and greenhouse gas emissions,vegetable waste composting was performed for 27 days using 6 different treatments. In addition to the controls,five vegetable waste mixtures（0.77m^3 each） were treated with different amounts of the SP additive, namely, 5%, 10%,15%, 20% and 25%. The ammonia volatilization loss and greenhouse gas emissions were measured during composting.Results indicated that the SP additive significantly decreased the ammonia volatilization and greenhouse gas emissions during vegetable waste composting. The additive reduced the total NH_3 emission by 4.0% to 16.7%. The total greenhouse gas emissions（CO_2-eq） of all treatments with SP additives were decreased by 10.2% to 20.8%, as compared with the controls. The NH_3 emission during vegetable waste composting had the highest contribution to the greenhouse effect caused by the four different gases.The amount of NH_3（CO_2-eq）from each treatment ranged from 59.90 to 81.58 kg/t; NH_3（CO_2-eq） accounted for 69% to 77% of the total emissions from the four gases. Therefore, SP is a cost-effective phosphorus-based fertilizer that can be used as an additive during vegetable waste composting to reduce the NH_3 and greenhouse gas emissions as well as to improve the value of compost as a fertilizer.

长链非编码RNA H19、Neat1、Meg3、Gas5在梅山与杜洛克猪卵泡及各组织中的差异表达分析

为探讨lnc RNA在卵泡及组织中的表达规律,采用荧光定量RT-PCR检测lncRNA H19、Neat1、Meg3、Gas5基因在杜洛克与梅山猪各级卵泡和猪不同组织中的表达量变化。结果表明:H19基因在杜洛克猪各级卵泡S、M1、M2、L中的表达量分别是梅山猪的3.61、0.57、4.19、3.27倍,各级卵泡品种间差异极显著(P<0.01);Neat1基因在杜洛克猪各级卵泡中的表达量分别是梅山猪的3.94、1.16、1.63、1.44倍,其中S卵泡品种间差异极显著(P<0.01);Meg3基因在杜洛克猪各级卵泡中的表达量分别是梅山猪的2.78、1.12、10.65、1.41倍,S、M2卵泡品种间差异极显著(P<0.01);Gas5基因在杜洛克猪各级卵泡中的表达量分别是梅山猪的4.53、1.40、6.31、1.14倍,S、M2卵泡品种间差异极显著(P<0.01),M1卵泡中差异显著(P<0.05)。组织表达分析结果表明,各基因在下丘脑、垂体、子宫、卵巢等组织中均有表达。此研究表明,4个lnc RNA基因在杜洛克和梅山猪中表达量存在较大差异,可能影响卵泡的增殖、发育和成熟。

GAS5靶向miR-222-3p对牙周膜干细胞成骨分化的影响机制

成骨诱导hPDLSCs后,茜素红染色显示矿化增强GAS5表达升高(P<0.05),Runx2、ALP和OCN mRNA表达降低,miR-222-3p表达升高(P<0.05);miR-222-3p抑制剂可逆转GAS5低表达对hPDLSCs的作用(P<0.05)。提示hPDLSCs成骨分化后GAS5表达上调,下调GAS5可通过靶向调控miR-222-3p,抑制hPDLSCs成骨分化。

长链非编码RNA NEAT1通过miR-34a靶向GAS1激活PI3K-AKT信号通路对宫颈癌的增殖、侵袭和上皮细胞-间充质转化的影响研究

目的探讨长链非编码RNA(lncRNA)NEAT1对宫颈癌细胞增殖、侵袭和上皮细胞-间充质转化(EMT)的影响和机制研究。方法选取2019年10月购自上海生物细胞研究所的宫颈癌细胞株(Hela细胞)和人宫颈上皮永生化细胞株(H8细胞)作为研究对象。通过实时荧光定量聚合酶链反应(RT-qPCR)检测NEAT1在Hela细胞和H8细胞中的差异表达,分析NEAT1对Hela细胞增殖、侵袭和EMT的影响。miRanda和双荧光素酶报告基因实验分析NEAT1和miR-34a之间的关系,检测NEAT1通过miR-34a对Hela细胞增殖、侵袭与EMT的影响,TargetScan和双荧光素酶报告基因实验分析miR-34a和GAS1的关系,检测miR-34靶向GAS1激活PI3K-AKT信号通路对Hela细胞增殖、侵袭和EMT的影响。分别用MTT、细胞侵袭实验和Western blot检测细胞的增殖、侵袭和EMT能力。结果 Hela细胞中NEAT1的表达上调(P<0.01),miR-34a表达下调(P<0.01),敲低NEAT1抑制了Hela细胞增殖、侵袭与EMT,NEAT1 3′UTR与miR-34a特异性结合。同时敲低NEAT1和miR-34a的表达,NEAT1表达的下调能部分逆转miR-34a下调对细胞功能的影响。同时过表达NEAT1和miR-34a, NEAT1能部分逆转miR-34a上调对细胞功能的影响。miR-34a和GAS13′UTR特异性结合,敲低GAS1抑制了Hela细胞增殖、侵袭与EMT,干扰GAS1抑制了Hela细胞p-PI3K、p-AKT蛋白的表达。和干扰miR-34a相比,同时抑制GAS1和miR-34a的表达能够逆转p-PI3K、p-AKT蛋白的上升。MK2206抑制了Hela细胞增殖、侵袭与EMT,而IGF-1促进了细胞的增殖、侵袭与EMT。结论 NEAT1通过miR-34a靶向GAS1激活了PI3K-AKT信号通路,从而促进宫颈癌的发展。

LncRNA GAS5通过TIMP-3启动子甲基化促进软骨胶原蛋白降解的实验研究

目的骨关节炎(osteoarthritis,OA)是一种常见的关节疾病,可由内源性和表观遗传因素引起。软骨退变可恶化为OA。本研究旨在探讨长链非编码RNA(long non-coding RNA,LncRNA)GAS5在OA中的基本机制。方法14例正常膝关节软骨组织取自2018年7月至2020年7月因胫骨平台骨折在复旦大学附属中山医院吴淞医院行骨折内固定的患者,男8例,女6例;年龄19~40岁,平均(31±5)岁。35例OA膝关节软骨组织取自2018年7月至2020年7月在复旦大学附属中山医院吴淞医院就诊,诊断为OA且有膝关节置换手术指征的患者,男17例,女18例;年龄62~86岁,平均年龄(70±4)岁。采用实时荧光定量聚合酶链式反应(real time-quantitative polymerase chain reaction,RT-qPCR)检测金属蛋白酶抑制剂(tissue inhibitor of metalloproteinase,TIMP)-3 mRNA的表达情况。使用白细胞介素(interleukin,IL)-1β(10 ng/mL)建立OA细胞模型,分析OA基因芯片GSE16464。RT-qPCR和western-blot检测软骨分解代谢抑制因子TIMP-3的表达。用芯片定量聚合酶链反应(quantitative polymerase chain reaction,qPCR)检测TIMP-3启动子区富集的CpG岛情况。用生物信息学方法预测GAS5与TIMP-3的靶向关系,并用双荧光素酶报告基因检测法进行验证。结果在OA患者组织中TIMP-3 mRNA表达显著低于正常软骨组织,OA患者组与正常软骨组织软骨细胞TIMP-3启动子区甲基化阳性率分别为72.1%和35.7%。LncRNAGAS5在OA中高表达,LncRNAGAS5在OA中表达较正常软骨组织中表达明显上调(P<0.05)。Blast序列比对GAS5序列与TIMP-3启动子序列具有结合位点。沉默LncRNA GAS5促进TIMP-3表达,抑制OA软骨细胞胶原降解。双荧光素酶报告基因实验验证GAS5与TIMP-3具有靶向调控关系。结论OA软骨组织中TIMP-3低表达,而LncRNA GAS5表达上调;LncRNA GAS5可富集DNA甲基化转移酶,抑制TIMP-3的表达,促进软骨细胞胶原蛋白降解。

The effective ionization coefficients and electron drift velocities in gas mixtures of CF_3I with N_2 and CO_2 obtained from Boltzmann equation analysis

The electron swarm parameters including the density-normalized effective ionization coefficients（α-η）/N and the electron drift velocities V e are calculated for a gas mixture of CF3I with N2 and CO2 by solving the Boltzmann equation in the condition of a steady-state Townsend（SST） experiment.The overall density-reduced electric field strength is from 100 Td to 1000 Td（1 Td = 10-17V·cm2）,while the CF3I content k in the gas mixture can be varied over the range from 0% to 100%.From the variation of（αη）/N with the CF3I mixture ratio k,the limiting field strength（E/N） lim for each CF3I concentration is derived.It is found that for the mixtures with 70% CF3I,the values of（E/N） lim are essentially the same as that for pure SF 6.Additionally,the global warming potential（GWP） and the liquefaction temperature of the gas mixtures are also taken into account to evaluate the possibility of application in the gas insulation of power equipment.

Methane Oxidation to Synthesis Gas Using Lattice Oxygen of La_(1-x)Sr_xMO_(3-λ)(M =Fe,Mn) Perovskite Oxides Instead of Molecular Oxygen

In this paper, the partial oxidation of methane to synthesis gas using lattice oxygen of La1- SrxMO3-λ (M=Fe, x Mn) perovskite oxides instead of molecular oxygen was investigated. The redox circulation between 11% O2/Ar flow and 11% CH4/He flow at 900℃ shows that methane can be oxidized to CO and H2 with a selectivity of over 90.7% using the lattice oxygen of La1- SrxFeO3-λ (x≤0.2) perovskite oxides in an appropriate reaction condition, while the lost lattice x oxygen can be supplemented by air re-oxidation. It is viable for the lattice oxygen of La1- SrxFeO3-λ (x≤0.2) perovskite x oxides instead of molecular oxygen to react with methane to synthesis gas in the redox mode.