Effect of Phosphorus-32 Glass Microspheres on the Human Hepatocellular Carcinoma in Nude Mice

Objective To study anticancer effect and ultrastructural influence of phosphorus 32 glass microspheres( 32 P GMS) injected in the hepatocellular carcinoma in nude mice. MethodsThe ultrastructural changes of tumor in both the treatment group and control group were examined by transmission electron microscope. ResultsIn the treatment group,a large number of tumor cells were killed and the death rate of tumor cells was much higher(35%-70%). Ultrastructurally, severe nuclear damage was observed in the dead cells. The early characteristics of necrosis such as margination of heterochromatin were also found in some tumor cells. Besides,well differentiated tumor cells, degenerative tumor cells and some lymphocytes were seen. The skin and muscle close to the tumor were normal. In the control group,the tumor consisted of poor differentiated tumor cells,in which there were only a few appototic cells(5%). ConclusionThe results suggest that the local administration of 32 P GMS produces obviously the anticancer effect.

Toxicology of intrahepatic arterial administration of interventional phosphorus-32 glass microspheres to domestic pigs

Objective To evaluate the toxic response to intrahepatic arterial admin istration of radioactive phosphorus32 glass microspheres (32 P GMS) in domestic pigs Methods Through selective catheterization of hepatic artery,32P GMS was infused to 5 healthy domestic pigs in a dosage equivalent to the thera peutic dose for human being, and31PGMS was infused to other 5 healthy domestic pigs Two pigs se rved as the whole course blank controls One pig from each group was surrendere d to euthanasia at week 1, 2, 4, 8 and 16, respectively, and liver biopsies were performed on the rest of pigs at the corresponding time points Liver tissues f r om different sites were taken for light and electron microscopy The ultrastruc t ural histopathological changes were evaluated semiquantitatively Results The accumulative amount of32PGMS in the target tissue attained more th an 90% of the total dose administrated Histologically, abnormal hepatocytes we re easily found at week 1 or 2 At week 4 they were less than at week 1 or 2, a n d endothelium of the sinuses were damaged prominently At week 8 they were scar cely seen, and liver tissue recovered gradually The histological features of l i ver tissue restored to normal at week 16 Semiquantitative analysis of ultrastr u ctural morphology in the experimental group showed no statistical difference ( P >050) between the nuclear abnormality (Nabn) and mitochrondri a l variability (Mvar) at week 1 or 2, but revealed prominent difference ( P <001, P <0001) as compared with those in other groups I n the experimental group the Nabn in tissues showed no significant differe nce ( P >020) between week 8 and week 16 Conclusion 32 PGMS internal irradiation at the dosag e equiva lent to human therapeutic dosage exerts reversible injury to domestic pig liver tissue, and it takes more than 8 weeks for the injured liver tissue to recover