3D printing of functional bioengineered constructs for neural regeneration: a review

Three-dimensional(3D)printing technology has opened a new paradigm to controllably and reproducibly fabricate bioengineered neural constructs for potential applications in repairing injured nervous tissues or producing in vitro nervous tissue models.However,the complexity of nervous tissues poses great challenges to 3D-printed bioengineered analogues,which should possess diverse architectural/chemical/electrical functionalities to resemble the native growth microenvironments for functional neural regeneration.In this work,we provide a state-of-the-art review of the latest development of 3D printing for bioengineered neural constructs.Various 3D printing techniques for neural tissue-engineered scaffolds or living cell-laden constructs are summarized and compared in terms of their unique advantages.We highlight the advanced strategies by integrating topographical,biochemical and electroactive cues inside 3D-printed neural constructs to replicate in vivo-like microenvironment for functional neural regeneration.The typical applications of 3D-printed bioengineered constructs for in vivo repair of injured nervous tissues,bio-electronics interfacing with native nervous system,neural-on-chips as well as brain-like tissue models are demonstrated.The challenges and future outlook associated with 3D printing for functional neural constructs in various categories are discussed.

3D printing of tissue engineering scaffolds:a focus on vascular regeneration

Tissue engineering is an emerging means for resolving the problems of tissue repair and organ replacement in regenerative medicine.Insufficient supply of nutrients and oxygen to cells in large-scale tissues has led to the demand to prepare blood vessels.Scaffold-based tissue engineering approaches are effective methods to form new blood vessel tissues.The demand for blood vessels prompts systematic research on fabrication strategies of vascular scaffolds for tissue engineering.Recent advances in 3D printing have facilitated fabrication of vascular scaffolds,contributing to broad prospects for tissue vascularization.This review presents state of the art on modeling methods,print materials and preparation processes for fabrication of vascular scaffolds,and discusses the advantages and application fields of each method.Specially,significance and importance of scaffold-based tissue engineering for vascular regeneration are emphasized.Print materials and preparation processes are discussed in detail.And a focus is placed on preparation processes based on 3D printing technologies and traditional manufacturing technologies including casting,electrospinning,and Lego-like construction.And related studies are exemplified.Transformation of vascular scaffolds to clinical application is discussed.Also,four trends of 3D printing of tissue engineering vascular scaffolds are presented,including machine learning,near-infrared photopolymerization,4D printing,and combination of self-assembly and 3D printing-based methods.

Runout prediction of potential landslides based on the multi-source data collaboration analysis on historical cases

Long runout landslides involve a massive amount of energy and can be extremely hazardous owing to their long movement distance,high mobility and strong destructive power.Numerical methods have been widely used to predict the landslide runout but a fundamental problem remained is how to determine the reliable numerical parameters.This study proposes a framework to predict the runout of potential landslides through multi-source data collaboration and numerical analysis of historical landslide events.Specifically,for the historical landslide cases,the landslide-induced seismic signal,geophysical surveys,and possible in-situ drone/phone videos(multi-source data collaboration)can validate the numerical results in terms of landslide dynamics and deposit features and help calibrate the numerical(rheological)parameters.Subsequently,the calibrated numerical parameters can be used to numerically predict the runout of potential landslides in the region with a similar geological setting to the recorded events.Application of the runout prediction approach to the 2020 Jiashanying landslide in Guizhou,China gives reasonable results in comparison to the field observations.The numerical parameters are determined from the multi-source data collaboration analysis of a historical case in the region(2019 Shuicheng landslide).The proposed framework for landslide runout prediction can be of great utility for landslide risk assessment and disaster reduction in mountainous regions worldwide.

A 3D basin modeling study of the factors controlling gas hydrate accumulation in the Shenhu Area of the South China Sea

Great advancement has been made on natural gas hydrates exploration and test production in the northern South China Sea.However,there remains a lot of key questions yet to be resolved,particularly about the mechanisms and the controls of gas hydrates enrichment.Numerical simulaution would play signficant role in addressing these questions.This study focused on the gas hydrate exploration in the Shenhu Area,Northern South China Sea.Based on the newly obtained borehole and multichannel reflection seismic data,the authors conducted an integrated 3D basin modeling study on gas hydrate.The results indicate that the Shenhu Area has favorable conditions for gas hydrate accumulation,such as temperature,pressure,hydrocarbon source,and tectonic setting.Gas hydrates are most concentrated in the Late Miocene strata,particularly in the structual highs between the Baiyun Sag and the Liwan Sag,and area to the south of it.It also proved the existence of overpressure in the main sag of source rocks,which was subject to compaction disequilibrium and hydrocarbon generation.It also shown that the regional fault activity is not conducive to gas hydrate accumulation due to excess gas seepage.The authors conjecture that fault activity may slightly weaken overpressure for the positive effect of hydrocarbon expulsion and areas lacking regional fault activity have better potential.

Application of Digital Technology in Road and Bridge Design

With the development and progress of science and technology,road and bridge design has experienced rapid development,from the initial manual drawing design to the popularity of Computer-Aided Design(CAD),and then to today’s digital software design era.Early designers relied on hand-drawn paper design forms which was time-consuming and error-prone.Digital support for road and bridge design not only saves the design time but the design quality has also achieved a qualitative leap.This paper engages in the application of digital technology in road and bridge design,to provide technical reference for China’s road and bridge engineering design units,to promote the popularity of Civil3D and other advanced design software in the field of engineering design and development,ultimately contributing to the sustainable development of China’s road and bridge engineering.

1D/3D Coupling Calculation Analysis on Bus Cooling System

Compared with front engine vehicle, the windward side’s flow field in cooling model of rear engine bus is complicated and it can’t be calculated by means of 1D model. For this problem, this paper has used Star-CCM to build a 3D simulation model of cooling system, engine compartment and complete vehicle. Then, it had a 1D/3D coupling calculation on cooling system with Kuli software. It could be helpful in the optimization design of the flow field of rear engine compartment and optimization match of cooling system.

Statistical Model of the 3-D Braided Composites Strength

Based on the statistical model for the tensile statistical strength of unidirectional composite materials and the stress analysis of 3-D braided composites, a new method is proposed to calculate the tensile statistical strength of the 3-D braided compos- ites. With this method, the strength of 3-D braided composites can be calculated with very large accuracy, and the statistical parameters of 3-D braided composites can be determined. The numerical result shows that the tensile statistical strength of 3-D braided composites can be predicted using this method.

基于改进BiLSTM的电力工程数据智能分析算法设计

针对目前电力工程费用计算复杂、时间成本较高且准确性低的问题,文中开展了基于改进BiLSTM的电力工程数据智能分析算法设计研究。从技术、工程量和费用三个维度构建了电力工程数据智能分析指标体系,进而提出了一种基于BiLSTM与Attention联合模型的电力工程费用预测算法。该算法将电力工程数据作为BiLSTM的模型输入,并采用Attention机制提高了对重要数据的关注程度。通过引入数据指标与电力工程费用的自动关联分析技术,实现了对电力工程费用的精准预测。仿真算例分析结果表明,与LSTM及BiLSTM算法相比,所提算法具有更高的预测准确性,平均预测误差小于5%。

FDM 3D打印技术在机械专业教学中的应用探讨

针对传统机械专业教学中教学方式平面化、三维教具制作成本高等问题,探讨了将低成本熔融沉积(FDM)3D打印技术应用于机械专业教学的可行性。以专业课程教具设计与制造为例,分析了3D打印应用的流程与成本。分析表明,在机械专业教学中应用3D打印技术可以实现教具的特殊性设计和快速低成本制造,将传统的二维教学模式转变为三维模式,从而激发学生积极性,提高教学质量。

软件工程化方法在武器3D实体建模中的应用研究

针对复杂武器3D实体建模的需求,分析并比较了软件工程化中的瀑布模型、变换模型和增量模型等3种模型开发方法,首次将增量模型方法引入到武器3D实体建模中。基本思路是,首先根据需求分析得到的信息将武器3D实体模型划分为不同的增量构件,然后对增量构件进行详细设计并完成基于MultiGen Creator的构件开发与测试,最后进行增量构件组装和模型测试。结果表明,应用该方法有利于提高模型的可重用性和开发效率,同时使开发的模型满足视景仿真系统的逼真性和实时性的要求。

基于虚拟现实技术的3D智慧校园设计与实现

以上海海洋大学为例,基于虚拟现实技术设计出与现实校园相近逼真的虚拟环境,用户通过计算机的操控与该虚拟环境进行交互,实现对智慧校园的控制。利用Auto Maya、Photoshop、Illustrator等建模、美化软件,进行校园建筑数据获取、3D模型的建立以及贴图纹理的映射等;利用Unity 3D引擎进行用户交互设计,建立起大学实际仿真景观的3D智慧校园。在此基础上,还实现了小地图的展示、界面多参数的调控、界面定位等个性化功能。

建立3D标准图样重构工程制图内容体系

简要讨论了三维(3D)设计时代的现实需要与工程制图国标体系之间的差异,分析了只有建立3D图样标准体系,才能体现3D设计的核心理念以及符合3D设计时代的需要,才能真正建立以3D造型为核心的现代工程制图内容体系。提出了由3D造型、画法几何、制图基础、专业制图、机械设计常识等5个模块要素组成的工程制图课程内容体系,并给出了32学时工程制图课程体系的具体内容与要求作为例证。

3D integrated modeling approach to geo-engineering objects of hydraulic and hydroelectric projects

Aiming at 3D modeling and analyzing problems of hydraulic and hydroelectric en-gineering geology,a complete scheme of solution is presented. The first basis was NURBS-TIN-BRep hybrid data structure. Then,according to the classified thought of the object-oriented technique,the different 3D models of geological and engi-neering objects were realized based on the data structure,including terrain class,strata class,fault class,and limit class;and the modeling mechanism was alterna-tive. Finally,the 3D integrated model was established by Boolean operations be-tween 3D geological objects and engineering objects. On the basis of the 3D model,a series of applied analysis techniques of hydraulic and hydroelectric engineering geology were illustrated. They include the visual modeling of rock-mass quality classification,the arbitrary slicing analysis of the 3D model,the geological analysis of the dam,and underground engineering. They provide powerful theoretical prin-ciples and technical measures for analyzing the geological problems encountered in hydraulic and hydroelectric engineering under complex geological conditions.

Evaluation of urban underground space resources using a negative list method: Taking Xi'an City as an example in China

Utilization of urban underground space has become a vital approach to alleviate the strain on urban land resources,and to optimize the structure and pattem of the city.It is also very important to improve the city environment,build livable city and increase the capacity of the city.Based on the analysis of existing evaluation methods and their problems,a method for evaluating underground space resources based on a negative list of adverse factors affecting underground space development is proposed,to be primarily used in urban planning stages.A list of the adverse factors is established,including limiting factors,constraining factors and influencing factors.Taking Xi'an as an example,using a geographical information system platform,a negative list of adverse factors for the underground space resources in Xi'an City are evaluated,and preventive measures are proposed.Natural resources,exploitable resources,and the potential growth of exploitable underground space resources are evaluated.Underground space assessment in the different development stages of the city,collaborative utilization and safety evaluation for multiple subsurface resources,environmental impact and assessment,as well as evaluation methods based on big data and intelligent optimization algorithms are all discussed with the aim of serving city planning and construction.

Investigation and numerical simulation of summer sedimentation in Jiaozhou Bay, China

In recent years,development activities have had a significant impact on the environment of the Jiaozhou Bay,China.To ensure the sustainable economic and social development of the Jiaozhou Bay area,it is necessary to strengthen corresponding control measures.The important prerequisite is to properly understand the environmental conditions laws of natural change,especially the dynamic processes of sediment and the characteristics of landform evolution.Based on the data of continuous observation at 6 stations in Jiaozhou Bay for 25 hours,the Hydrodynamic Eutrophication Model(HEM-3D)was used to simulate the sediment erosion and deposition.The results show that the maximum suspended sediment concentration in the sea area of Jiaozhou Bay is about 40 mg/L,which appears in the northwestern area of the bay top and the Cangkou watercourse area,and the low concentration is located in the area of the central Jiaozhou Bay towards the bay mouth.The suspended sediment is 6?10 mg/L.Affected by a decrease in seawater material,the direction of the prevailing current in the Jiaozhou Bay area is different from that of the sediment transport.The velocity of the flood current is higher than that of the ebb current.However,during flood tide,the flux of resuspended seafloor sediment outside and at the mouth of the bay is limited and cannot contribute significantly to the suspended sediment in the bay.During ebb tide,the resuspended sediment at the shallow-water bay head and the east and west sides spreads toward the bay mouth with the ebb current,although it extends beyond the bay through the bay mouth.The research results can provide scientific support for the Jiaozhou Bay project construction and environmental protection.

Reverse Engineering Based Methodology for Redesigning Contour Milling Tools

Reverse Engineering (RE) involves the use of techniques aimed to retrieve information about manufactured products, not only regarding geometries, but also materials and functionality. Today, even if several RE techniques are known, many of them still leave the object unusable to analyze. Nevertheless, other alternatives to this problem allows for obtaining a Digital or Virtual Model (VM) via the three-dimen- sional scanning. Getting the VM of an item, via scanning or not, can offer many possibilities to digital analysis (FEM). Furthermore, starting from VM, it is possible to achieve the physical reproduction of an element, part or workpiece—in the same or different materials—using Additive Manufacturing (AM) technologies. This enables to improve the product through a redesign process. In this paper, a RE based methodology is proposed for redesigning a tool for contour milling, after comparing different microscopy based techniques, 3D-Scanner tools and CAD-CAGD utilities for generating a Virtual Model of the newly designed mill.

Research on BIM Model Reshaping Method Based on 3D Point Cloud Recognition

In view of the limitations of traditional measurement methods in the field of building information,such as complex operation,low timeliness and poor accuracy,a new way of combining three-dimensional scanning technology and BIM(Building Information Modeling)model was discussed.Focused on the efficient acquisition of building geometric information using the fast-developing 3D point cloud technology,an improved deep learning-based 3D point cloud recognition method was proposed.The method optimised the network structure based on RandLA-Net to adapt to the large-scale point cloud processing requirements,while the semantic and instance features of the point cloud were integrated to significantly improve the recognition accuracy and provide a precise basis for BIM model remodeling.In addition,a visual BIM model generation system was developed,which systematically transformed the point cloud recognition results into BIM component parameters,automatically constructed BIM models,and promoted the open sharing and secondary development of models.The research results not only effectively promote the automation process of converting 3D point cloud data to refined BIM models,but also provide important technical support for promoting building informatisation and accelerating the construction of smart cities,showing a wide range of application potential and practical value.

Development of a multicellular 3D-bioprinted microtissue model of human periodontal ligament-alveolar bone biointerface:Towards a pre-clinical model of periodontal diseases and personalized periodontal tissue engineering

While periodontal (PD) disease is among principal causes of tooth loss worldwide, regulation of concomitant soft and mineralized PD tissues, and PD pathogenesis have not been completely clarified yet. Besides, relevant pre-clinical models and in vitro platforms have limitations in simulating human physiology. Here, we have harnessed three-dimensional bioprinting (3DBP) technology for developing a multi-cellular microtissue model resembling PD ligament-alveolar bone (PDL-AB) biointerface for the first time. 3DBP parameters were optimized;the physical, chemical, rheological, mechanical, and thermal properties of the constructs were assessed. Constructs containing gelatin methacryloyl (Gel-MA) and hydroxyapatite-magnetic iron oxide nanoparticles showed higher level of compressive strength when compared with that of Gel-MA constructs. Bioprinted self-supporting microtissue was cultured under flow in a microfluidic platform for >10 days without significant loss of shape fidelity. Confocal microscopy analysis indicated that encapsulated cells were homogenously distributed inside the matrix and preserved their viability for >7 days under microfluidic conditions. Immunofluorescence analysis showed the cohesion of stromal cell surface marker-1+ human PDL fibroblasts containing PDL layer with the osteocalcin+ human osteoblasts containing mineralized layer in time, demonstrating some permeability of the printed constructs to cell migration. Preliminary tetracycline interaction study indicated the uptake of model drug by the cells inside the 3D-microtissue. Also, the non-toxic levels of tetracycline were determined for the encapsulated cells. Thus, the effects of tetracyclines on PDL-AB have clinical significance for treating PD diseases. This 3D-bioprinted multi-cellular periodontal/osteoblastic microtissue model has potential as an in vitro platform for studying processes of the human PDL.

Additive manufacturing technology in mining engineering research

Nowadays,additive manufacturing,or 3D printing(3DP),though not a new technology in many industrial fields,is still relatively novel in mining engineering.This study explored the application of 3D metal printing,3D polylactic acid/acrylonitrile butadiene styrene printing,and 3D concrete printing in coal mining.Some examples of physical models established via 3DP technologies were studied in detail,namely,3DP bolts,3DP steel ladder beams,3DP face plates,and 3DP metal arches,which were installed in scaled 3DP concrete prototype models of coal mine excavation.Through the comprehensive laboratory loading tests,the models could simulate the damage scenario highly similar to the real failures.The results show that the 3D printed physical models greatly improved the accuracy and reliability of experiments.On this basis,the conceptual design of a 3DP machine for physical models was proposed,which had the potential of resembling the strata deformation/collapse in natural scenarios.Through assessing the applicability of the additive manufacturing technology in mining engineering,this study aimed to further explore its potential applicability in other engineering contexts such as slope controlling,large underground engineering,and underground space stability.

某矿山公园3D漫游系统设计及实现

以某矿山公园的二维矢量数据为基础,采用Google SketchUp6.0软件平台,通过三维模型构建、渲染及漫游定制等过程,初步构建了一个矿山公园三维GIS系统实验原型,实现了与ArcGIS软件之间的交互,结合ArcGIS软件进行三维矿山公园场景驱动的开发工作,形成了矿山公园的三维建模场景再现,在Google SketchUp6.0中实现的建模效果与ArcGIS Engine中各种控件的浏览漫游功能统一起来,开发了一个简单的矿山公园三维漫游系统。