In this paper, we fabricated three kinds of 3D microgrooves with different depth on biocompatible poly（lactic-co-glycolic acid） （PLGA） substrate via combination of soft-lithography and melt-casting methods, and in...In this paper, we fabricated three kinds of 3D microgrooves with different depth on biocompatible poly（lactic-co-glycolic acid） （PLGA） substrate via combination of soft-lithography and melt-casting methods, and investigated in detail their influence on C2C12 cell behaviors. It is found that cell proliferation, migration, alignment, spatial distribution, F-actin protein expression and gene expression are all remarkably distinct on these microgrooved samples and the smooth control PLGA substrate. The associated underlying mechanisms were further analyzed and discussed using real-time living cell monitoring, confocal laser scanning microscopy and gene microarray. Our preliminary results suggested that 3D microstruc- ture could affect cell behaviors in a much more extensive manner than what we had understood before.展开更多
基金supported by the National Basic Research Program of China(Nos.2012CB619100 and 2011CB606204)the National Natural Science Foundation of China(Nos.51373056,51232002 and 51372085)+1 种基金the“111 Project”(No.B13039)the Fundamental Research Funds for the Central Universities
文摘In this paper, we fabricated three kinds of 3D microgrooves with different depth on biocompatible poly（lactic-co-glycolic acid） （PLGA） substrate via combination of soft-lithography and melt-casting methods, and investigated in detail their influence on C2C12 cell behaviors. It is found that cell proliferation, migration, alignment, spatial distribution, F-actin protein expression and gene expression are all remarkably distinct on these microgrooved samples and the smooth control PLGA substrate. The associated underlying mechanisms were further analyzed and discussed using real-time living cell monitoring, confocal laser scanning microscopy and gene microarray. Our preliminary results suggested that 3D microstruc- ture could affect cell behaviors in a much more extensive manner than what we had understood before.
