Keratinocytes and fibroblasts,derived from hiPSCs,were used to construct the human epidermal model by a culture patch made by monolayer poly-(lactic-co-glycolic acid)(PLGA)nanofibers and a human skin-on-a-chip device....Keratinocytes and fibroblasts,derived from hiPSCs,were used to construct the human epidermal model by a culture patch made by monolayer poly-(lactic-co-glycolic acid)(PLGA)nanofibers and a human skin-on-a-chip device.Unlike the conventional culture dish method,two different epidermal cells are successfully adhered to the front and back sides of the patch,which produces a three-dimensional nanofibrous scaffold similar to a natural extracellular matrix before the patch was cultured in the skin-on-a-chip device to mimic the physiological conditions of human skin.As expected,the differentiated hiPSCs show the expression of keratinocyte-and fibroblast-specific proteins on the patch,and the layering is found between these two kinds of cells,indicating that this approach creates a powerful in vitro system for modeling skin development and diseases.展开更多
Via the use of Mimedisc~? as a scaffold, the normal human dermal fibroblasts were first seeding into the scaffold to form a dermal equivalent after three weeks, and then seeding normal human epidermal keratinocytes on...Via the use of Mimedisc~? as a scaffold, the normal human dermal fibroblasts were first seeding into the scaffold to form a dermal equivalent after three weeks, and then seeding normal human epidermal keratinocytes on it, followed by immersion and air-liquid interphase culture stage, to finally form a 3 D sponge matrix skin model which has a very similar structure of normal human skin(NHS). The results of Masson staining, and immunohistochemistry(IHC), immunofluorescence(IF) staininccg show that this skin model has a very similar structure and key protein expression of NHS. Meanwhile, this model was used as a tool to investigate the effect of Himalaya cedar extract on Dermal-Epidermal Junction(DEJ) in vitro. Results show that the Himalayan cedar extract can significantly increase the thickness of the epidermis, thus increase the barrier capacity of the skin to resist external environment. Further, this Himalaya cedar extract can promote the expressions of Laminin 5 and Collagen VII of DEJ. The expressions of the Laminin 5 and Collagen VII increased 20% and 47% respectively.So the Himalaya cedar extract displays the potential to be applied in development of anti-aging cosmetics.展开更多
Skin is the largest organ in human body,and it plays an important role in regulating physiological microenvironments and acts as a barrier to protect human body from harmful intrusions.The demand for fully functional ...Skin is the largest organ in human body,and it plays an important role in regulating physiological microenvironments and acts as a barrier to protect human body from harmful intrusions.The demand for fully functional skin models(also called skin equivalents,SE)in an in-vivo mimicking culturing microenvironment has been increased dramatically due to the fast development in skin disease treatments and skin care products.Owing to the emerging of the concept and technology of organ-on-chips along with the three-dimensional(3D)bioprinting technology,3D skin models and their applications have been fast evolving.In this paper,the advances in the development of 3D skin models along with skin-on-a-chip(SOC)are reviewed and commented.One of the findings with this paper is that the SOC together with the 3D bioprinting technology is promising to construct fully functional 3D skin models in the field of pharmaceutical and cosmetic industries.展开更多
将十二烷基硫酸钠(SDS)和不同分子量的透明质酸(HA)共同作用于人永生化表皮细胞(Ha Ca T)和3D皮肤模型,作用24 h后通过MTT比色法检测细胞活性,利用ELISA方法检测培养液中人白细胞介素(IL-1α)的水平,并进行组织学观察细胞形态。研究在...将十二烷基硫酸钠(SDS)和不同分子量的透明质酸(HA)共同作用于人永生化表皮细胞(Ha Ca T)和3D皮肤模型,作用24 h后通过MTT比色法检测细胞活性,利用ELISA方法检测培养液中人白细胞介素(IL-1α)的水平,并进行组织学观察细胞形态。研究在不同实验模型中,不同分子量HA影响SDS对细胞的损伤和释放IL-1α的差异。结果表明,3D皮肤模型可用于HA的体外抗皮肤刺激作用的研究,实验结果更接近人体实际使用情况以及能提供更多的机制信息。展开更多
基金by the National Natural Science Foundation of China(Nos.31600801 and 11804121)the Natural Science Foundation of Hubei(No.2019CFB558)the Central Government Guides the Special Fund Project of Local Scientific and Technological Development(No.[2016]109)。
文摘Keratinocytes and fibroblasts,derived from hiPSCs,were used to construct the human epidermal model by a culture patch made by monolayer poly-(lactic-co-glycolic acid)(PLGA)nanofibers and a human skin-on-a-chip device.Unlike the conventional culture dish method,two different epidermal cells are successfully adhered to the front and back sides of the patch,which produces a three-dimensional nanofibrous scaffold similar to a natural extracellular matrix before the patch was cultured in the skin-on-a-chip device to mimic the physiological conditions of human skin.As expected,the differentiated hiPSCs show the expression of keratinocyte-and fibroblast-specific proteins on the patch,and the layering is found between these two kinds of cells,indicating that this approach creates a powerful in vitro system for modeling skin development and diseases.
文摘Via the use of Mimedisc~? as a scaffold, the normal human dermal fibroblasts were first seeding into the scaffold to form a dermal equivalent after three weeks, and then seeding normal human epidermal keratinocytes on it, followed by immersion and air-liquid interphase culture stage, to finally form a 3 D sponge matrix skin model which has a very similar structure of normal human skin(NHS). The results of Masson staining, and immunohistochemistry(IHC), immunofluorescence(IF) staininccg show that this skin model has a very similar structure and key protein expression of NHS. Meanwhile, this model was used as a tool to investigate the effect of Himalaya cedar extract on Dermal-Epidermal Junction(DEJ) in vitro. Results show that the Himalayan cedar extract can significantly increase the thickness of the epidermis, thus increase the barrier capacity of the skin to resist external environment. Further, this Himalaya cedar extract can promote the expressions of Laminin 5 and Collagen VII of DEJ. The expressions of the Laminin 5 and Collagen VII increased 20% and 47% respectively.So the Himalaya cedar extract displays the potential to be applied in development of anti-aging cosmetics.
文摘Skin is the largest organ in human body,and it plays an important role in regulating physiological microenvironments and acts as a barrier to protect human body from harmful intrusions.The demand for fully functional skin models(also called skin equivalents,SE)in an in-vivo mimicking culturing microenvironment has been increased dramatically due to the fast development in skin disease treatments and skin care products.Owing to the emerging of the concept and technology of organ-on-chips along with the three-dimensional(3D)bioprinting technology,3D skin models and their applications have been fast evolving.In this paper,the advances in the development of 3D skin models along with skin-on-a-chip(SOC)are reviewed and commented.One of the findings with this paper is that the SOC together with the 3D bioprinting technology is promising to construct fully functional 3D skin models in the field of pharmaceutical and cosmetic industries.
文摘将十二烷基硫酸钠(SDS)和不同分子量的透明质酸(HA)共同作用于人永生化表皮细胞(Ha Ca T)和3D皮肤模型,作用24 h后通过MTT比色法检测细胞活性,利用ELISA方法检测培养液中人白细胞介素(IL-1α)的水平,并进行组织学观察细胞形态。研究在不同实验模型中,不同分子量HA影响SDS对细胞的损伤和释放IL-1α的差异。结果表明,3D皮肤模型可用于HA的体外抗皮肤刺激作用的研究,实验结果更接近人体实际使用情况以及能提供更多的机制信息。
