Background: Studies have shown a strong correlation between the growth of E2 in serum and estrone-3-glucuronide (E1-3G) in urine during ovarian stimulation. Thus, we developed theoretical models for using urinary E1-3...Background: Studies have shown a strong correlation between the growth of E2 in serum and estrone-3-glucuronide (E1-3G) in urine during ovarian stimulation. Thus, we developed theoretical models for using urinary E1-3G in ovarian stimulation and focused on their experimental verification and analysis. Methods: A prospective, observational pilot study was conducted involving 54 patients who underwent 54 cycles of ovarian stimulation. The goal was to establish the growth rate of urinary E1-3G during the course of stimulation and to determine the daily upper and lower limits of growth rates at which stimulation is appropriate and safe. Controlled ovarian stimulation was performed using two different stimulation protocols—an antagonist protocol in 25 cases and a progestin-primed ovarian stimulation protocol (PPOS) in 29 cases, with fixed doses of gonadotropins. From the second day of stimulation, patients self-measured their daily urine E1-3G levels at home using a portable analyzer. In parallel, a standard ultrasound follow-up protocol accompanied by a determination of E2, LH, and P levels was applied to optimally control stimulation. Results: The average daily growth rates in both groups were about 50%. The daily increase in E1-3G for the antagonist protocol ranged from 14% to 79%, while they were 28% to 79% for the PPOS protocol. Conclusion: This is the first study to analyze the dynamics of E1-3G in two different protocols and to estimate the limits of its increase during the entire course of the stimulation. The results confirm our theoretical model for the viability of using urinary E1-3G for monitoring ovarian stimulation.展开更多
OAM (Operations, Administration and Maintenance) system is a very impo rtant component of 3G cellular network. In order to acquire overall managemen t, fast response and steady operation, an SCTP (Stream Control Trans...OAM (Operations, Administration and Maintenance) system is a very impo rtant component of 3G cellular network. In order to acquire overall managemen t, fast response and steady operation, an SCTP (Stream Control Transmission Prot ocol) based OAM, i.e., SOAM system was proposed. SOAM implements new characters of SCTP such as multi-stream, enforced SACK and heartbeat mechanism on its tran sport layer. These characters help SOAM decrease the message transmission delay and accelerate the link failure detection. Besides, a new component named SOAM agent was introduced to improve the operation efficiency of SOAM. The experim ental results prove the proposed SOAM system achieves better performance on sign aling transmission compared with conventional TCP based OAM system.展开更多
视频流量逐渐在网络中占据主导地位,且视频平台大多对其进行加密传输。虽然加密传输视频可以有效保护用户隐私,但是也增加了监管有害视频传播的难度.现有的加密视频识别方法基于TCP(Transmission Control Protocol)传输协议头部信息和HT...视频流量逐渐在网络中占据主导地位,且视频平台大多对其进行加密传输。虽然加密传输视频可以有效保护用户隐私,但是也增加了监管有害视频传播的难度.现有的加密视频识别方法基于TCP(Transmission Control Protocol)传输协议头部信息和HTTP/1.1(Hypertext Transfer Protocol Version1.1)的传输模式,提取应用层音视频数据单元传输长度序列来实现视频识别.但是随着基于UDP(User Datagram Protocol)的QUIC(Quick UDP Internet Connections)协议及基于QUIC实现的HTTP/3(Hypertext Transfer Protocol Version 3)协议应用于视频传输,已有方法不再适用.HTTP/3协议缺少类似TCP的头部信息,且使用了多路复用机制,并对几乎所有数据进行了加密,此外,视频平台开始使用多片段合并分发技术,这给从网络流量中精准识别加密视频带来了巨大挑战。本文基于HTTP/3协议中的控制信息特征,提出了从HTTP/3加密视频流中提取数据传输特征并进行修正的方法,最大程度复原出应用层音视频长度特征.面向多片段合并分发导致的海量匹配问题,本文基于明文指纹库设计了键值数据库来实现视频的快速识别.实验结果表明,本文提出的基于HTTP/3传输特性的加密视频识别方法能够在包含36万个真实视频指纹的YouTube大规模指纹库中达到接近99%的准确率,100%的精确率以及99.32%的F1得分,对传输过程中加人了填充顿的Facebook平台,在包含28万个真实视频指纹的大规模指纹库中达到95%的准确率、100%的精确率以及96.45%的F1得分,在具有同样特性的Instagram平台中,最高可达到97.57%的F1得分,且本方法在所有指纹库中的平均视频识别时间均低于0.4秒.本文的方法首次解决了使用HTTP/3传输的加密视频在大规模指纹库场景中的识别问题,具有很强的实用性和通用性.展开更多
3D （Three-dimensional） Caco-2 spheroids closely recapitulating in vivo physiological organization of intestinal epithelial cells, provide an excellent in vitro model system to study their pathophysiology and their r...3D （Three-dimensional） Caco-2 spheroids closely recapitulating in vivo physiological organization of intestinal epithelial cells, provide an excellent in vitro model system to study their pathophysiology and their response to stressful stimuli. The objective of this technical note is to provide optimized in vitro experimental protocols for culturing 3D Caco-2 spheroids and for analyzing their cell growth features. An optimized 3D Caco-2 spheroid culturing technique based on a new configuration of the culture medium is provided A methodological approach to determine the distribution of the cell cycle phases in disaggregated Caco-2 spheroids by using cytofluorimetric analysis is also described. The optimized culturing protocol favors 3D Caco-2 spheroid differentiation process, as evaluated by the number of well-differentiated spheroids with a single hollow lumen. The cytofluorimetric analysis allows rapid collection of cell cycle phase data from high numbers of spheroid samples, thus, permitting to estimate their growth dynamics in a relatively short time. The optimized technical approaches described here can be applied in systematic manner to a variety of research activities utilizing 3D Caco-2 spheroids. Ease of use, time and economic saving advantages deriving from these protocols further highlight their potential.展开更多
Background:The emergence of Coronavirus SARS-CoV-2 evoked an unprecedented threat globally.Ever since the spread of this pandemic research and clinical trials have concentrated on the repurposing of already exciting F...Background:The emergence of Coronavirus SARS-CoV-2 evoked an unprecedented threat globally.Ever since the spread of this pandemic research and clinical trials have concentrated on the repurposing of already exciting FDA drugs to find a successful candidate to combat Covid-19.Objective:The objective of this study is to propose a therapeutic protocol that may have a potential solution to treat the severe infections associated with coronavirus.The clinical application of this protocol(Al-Akidi Therapeutic Protocol,A-TP)is highly recommended,as there are several scientific evidences that support this trusted protocol to be of great potential.Methods and Materials:This A-TP includes the use of one of the respiratory Fluoroquinolones(Levofloxacin or Moxifloxacin)in doses of 500 mg twice daily for 7-10 days,together with high doses of Vitamin D3(10000 IU/day)and Zinc(50 mg daily)for few weeks.This protocol is based on the previous antiviral activity of those Fluoroquinolones towards few viruses,the potent antibacterial activity on respiratory infections and high available concentrations in the lungs.It is also based on molecular docking of Levofloxacin and Moxifloxacin on various viral enzymes.Results:Molecular docking showed encouraging and very interesting docking scores and binding affinity of Levofloxacin and Moxifloxacin to certain viral enzymes,such as,RNA dependent RNA polymerase(RdRp),3-Cysteine-Like protease,Neuraminidase,Replicase polyproteins and Trans-Membrane Protease Serine 2 inhibitor(TMPRSS2).The highly expected clinical results of using this protocol are:reduce infection,control of temperature,improve breathing with less dependent on supplemented oxygen,and remarkable reduction of the pro-inflammatory cytokine storm,and hence,reduce hospitalization and mortality.Conclusion:Levofloxacin is highly recommended in managing the severe infections associated with Corona virus and has a remarkable reduction of pro-inflammatory cytokine storm as an immuomodulating agent.Levofloxacin is superior in this protocol over Moxifloxacin,due to its high excretion(≤83%)as unchanged through the kidneys,while Moxifloxacin is only 20%is excreted unchanged.It is an extra advantage of Levofloxacin to manage coronavirus in the kidneys.High doses of Vitamin D3 and Zinc are very useful to provide additional effective measures to combat Corona virus.Therefore,the use of this A-TP is highly and strongly recommended,as it serves the full requirements for excellent and potential therapy for the severe infections associated with Covid-19.展开更多
电子邮件是Internet上应用最多和最广的服务项目,SMTP(Simple Mail Transport protocol)和POP3(Post office protocol)是在电子邮件服务中分别负责收信和发信的两种协议。本文介绍了电子邮件系统的工作原理及相关协议,并基于这些协议设...电子邮件是Internet上应用最多和最广的服务项目,SMTP(Simple Mail Transport protocol)和POP3(Post office protocol)是在电子邮件服务中分别负责收信和发信的两种协议。本文介绍了电子邮件系统的工作原理及相关协议,并基于这些协议设计和开发了一个实用的邮件系统。展开更多
文摘Background: Studies have shown a strong correlation between the growth of E2 in serum and estrone-3-glucuronide (E1-3G) in urine during ovarian stimulation. Thus, we developed theoretical models for using urinary E1-3G in ovarian stimulation and focused on their experimental verification and analysis. Methods: A prospective, observational pilot study was conducted involving 54 patients who underwent 54 cycles of ovarian stimulation. The goal was to establish the growth rate of urinary E1-3G during the course of stimulation and to determine the daily upper and lower limits of growth rates at which stimulation is appropriate and safe. Controlled ovarian stimulation was performed using two different stimulation protocols—an antagonist protocol in 25 cases and a progestin-primed ovarian stimulation protocol (PPOS) in 29 cases, with fixed doses of gonadotropins. From the second day of stimulation, patients self-measured their daily urine E1-3G levels at home using a portable analyzer. In parallel, a standard ultrasound follow-up protocol accompanied by a determination of E2, LH, and P levels was applied to optimally control stimulation. Results: The average daily growth rates in both groups were about 50%. The daily increase in E1-3G for the antagonist protocol ranged from 14% to 79%, while they were 28% to 79% for the PPOS protocol. Conclusion: This is the first study to analyze the dynamics of E1-3G in two different protocols and to estimate the limits of its increase during the entire course of the stimulation. The results confirm our theoretical model for the viability of using urinary E1-3G for monitoring ovarian stimulation.
基金High-Tech Research and DevelopmentProgram of China (No. 2003AA123310)
文摘OAM (Operations, Administration and Maintenance) system is a very impo rtant component of 3G cellular network. In order to acquire overall managemen t, fast response and steady operation, an SCTP (Stream Control Transmission Prot ocol) based OAM, i.e., SOAM system was proposed. SOAM implements new characters of SCTP such as multi-stream, enforced SACK and heartbeat mechanism on its tran sport layer. These characters help SOAM decrease the message transmission delay and accelerate the link failure detection. Besides, a new component named SOAM agent was introduced to improve the operation efficiency of SOAM. The experim ental results prove the proposed SOAM system achieves better performance on sign aling transmission compared with conventional TCP based OAM system.
文摘视频流量逐渐在网络中占据主导地位,且视频平台大多对其进行加密传输。虽然加密传输视频可以有效保护用户隐私,但是也增加了监管有害视频传播的难度.现有的加密视频识别方法基于TCP(Transmission Control Protocol)传输协议头部信息和HTTP/1.1(Hypertext Transfer Protocol Version1.1)的传输模式,提取应用层音视频数据单元传输长度序列来实现视频识别.但是随着基于UDP(User Datagram Protocol)的QUIC(Quick UDP Internet Connections)协议及基于QUIC实现的HTTP/3(Hypertext Transfer Protocol Version 3)协议应用于视频传输,已有方法不再适用.HTTP/3协议缺少类似TCP的头部信息,且使用了多路复用机制,并对几乎所有数据进行了加密,此外,视频平台开始使用多片段合并分发技术,这给从网络流量中精准识别加密视频带来了巨大挑战。本文基于HTTP/3协议中的控制信息特征,提出了从HTTP/3加密视频流中提取数据传输特征并进行修正的方法,最大程度复原出应用层音视频长度特征.面向多片段合并分发导致的海量匹配问题,本文基于明文指纹库设计了键值数据库来实现视频的快速识别.实验结果表明,本文提出的基于HTTP/3传输特性的加密视频识别方法能够在包含36万个真实视频指纹的YouTube大规模指纹库中达到接近99%的准确率,100%的精确率以及99.32%的F1得分,对传输过程中加人了填充顿的Facebook平台,在包含28万个真实视频指纹的大规模指纹库中达到95%的准确率、100%的精确率以及96.45%的F1得分,在具有同样特性的Instagram平台中,最高可达到97.57%的F1得分,且本方法在所有指纹库中的平均视频识别时间均低于0.4秒.本文的方法首次解决了使用HTTP/3传输的加密视频在大规模指纹库场景中的识别问题,具有很强的实用性和通用性.
文摘3D （Three-dimensional） Caco-2 spheroids closely recapitulating in vivo physiological organization of intestinal epithelial cells, provide an excellent in vitro model system to study their pathophysiology and their response to stressful stimuli. The objective of this technical note is to provide optimized in vitro experimental protocols for culturing 3D Caco-2 spheroids and for analyzing their cell growth features. An optimized 3D Caco-2 spheroid culturing technique based on a new configuration of the culture medium is provided A methodological approach to determine the distribution of the cell cycle phases in disaggregated Caco-2 spheroids by using cytofluorimetric analysis is also described. The optimized culturing protocol favors 3D Caco-2 spheroid differentiation process, as evaluated by the number of well-differentiated spheroids with a single hollow lumen. The cytofluorimetric analysis allows rapid collection of cell cycle phase data from high numbers of spheroid samples, thus, permitting to estimate their growth dynamics in a relatively short time. The optimized technical approaches described here can be applied in systematic manner to a variety of research activities utilizing 3D Caco-2 spheroids. Ease of use, time and economic saving advantages deriving from these protocols further highlight their potential.
基金The author acknowledges the help and support of Dr.Sabah J.Saleh(The National Center for Quality Control and Research,Ministry of Health(Iraq))and Ameer H.Khadem(Department of Pharmaceutical Chemistry,College of Pharmacy,Al-Bayan University).
文摘Background:The emergence of Coronavirus SARS-CoV-2 evoked an unprecedented threat globally.Ever since the spread of this pandemic research and clinical trials have concentrated on the repurposing of already exciting FDA drugs to find a successful candidate to combat Covid-19.Objective:The objective of this study is to propose a therapeutic protocol that may have a potential solution to treat the severe infections associated with coronavirus.The clinical application of this protocol(Al-Akidi Therapeutic Protocol,A-TP)is highly recommended,as there are several scientific evidences that support this trusted protocol to be of great potential.Methods and Materials:This A-TP includes the use of one of the respiratory Fluoroquinolones(Levofloxacin or Moxifloxacin)in doses of 500 mg twice daily for 7-10 days,together with high doses of Vitamin D3(10000 IU/day)and Zinc(50 mg daily)for few weeks.This protocol is based on the previous antiviral activity of those Fluoroquinolones towards few viruses,the potent antibacterial activity on respiratory infections and high available concentrations in the lungs.It is also based on molecular docking of Levofloxacin and Moxifloxacin on various viral enzymes.Results:Molecular docking showed encouraging and very interesting docking scores and binding affinity of Levofloxacin and Moxifloxacin to certain viral enzymes,such as,RNA dependent RNA polymerase(RdRp),3-Cysteine-Like protease,Neuraminidase,Replicase polyproteins and Trans-Membrane Protease Serine 2 inhibitor(TMPRSS2).The highly expected clinical results of using this protocol are:reduce infection,control of temperature,improve breathing with less dependent on supplemented oxygen,and remarkable reduction of the pro-inflammatory cytokine storm,and hence,reduce hospitalization and mortality.Conclusion:Levofloxacin is highly recommended in managing the severe infections associated with Corona virus and has a remarkable reduction of pro-inflammatory cytokine storm as an immuomodulating agent.Levofloxacin is superior in this protocol over Moxifloxacin,due to its high excretion(≤83%)as unchanged through the kidneys,while Moxifloxacin is only 20%is excreted unchanged.It is an extra advantage of Levofloxacin to manage coronavirus in the kidneys.High doses of Vitamin D3 and Zinc are very useful to provide additional effective measures to combat Corona virus.Therefore,the use of this A-TP is highly and strongly recommended,as it serves the full requirements for excellent and potential therapy for the severe infections associated with Covid-19.
文摘电子邮件是Internet上应用最多和最广的服务项目,SMTP(Simple Mail Transport protocol)和POP3(Post office protocol)是在电子邮件服务中分别负责收信和发信的两种协议。本文介绍了电子邮件系统的工作原理及相关协议,并基于这些协议设计和开发了一个实用的邮件系统。
