Objective: To investigate the effect of SLC6A8 on the proliferation of liver cancer cells by regulating mitophagy through BNIP3L. Methods: The expression of the SLC6A8 gene in liver cancer tissues was analyzed using t...Objective: To investigate the effect of SLC6A8 on the proliferation of liver cancer cells by regulating mitophagy through BNIP3L. Methods: The expression of the SLC6A8 gene in liver cancer tissues was analyzed using the TCGA database, and its correlation with BNIP3L expression was assessed. RT-PCR and Western blot techniques were employed to detect the expression of SLC6A8 and BNIP3L in human liver cancer Huh-7 and Hep3B cells. Immunofluorescence labeling and CCK-8 assay were used to observe mitochondrial autophagy and cell proliferation rate in SLC6A8-overexpressing Huh-7 and Hep3B cells. Evaluate the proliferation rate of SLC6A8-overexpressing Huh-7 and Hep3B cells after silencing BNIP3L using the CCK-8 detection method. Results: SLC6A8 was significantly overexpressed in liver cancer tissues and positively correlated with BNIP3L expression. Overexpression of SLC6A8 significantly promoted mitochondrial autophagy and proliferation of Huh-7 and Hep3B cells. Additionally, SLC6A8 overexpression significantly enhanced the expression of BNIP3L mRNA and protein. Upon BNIP3L silencing, the proliferative effect of SLC6A8 overexpression on liver cancer cells was reversed. Conclusion: High expression of SLC6A8 in liver cancer tissues is positively correlated with BNIP3L, and overexpression of SLC6A8 promotes mitochondrial autophagy and liver cancer cell proliferation. Silencing BNIP3L can reverses the effect of overexpression of SLC6A8 on liver cancer cell proliferation. This provides new targets and strategies for the treatment of liver cancer.展开更多
目的:观察川黄方治疗3~4期慢性肾脏病(CKD)患者的临床疗效,及对急性肾损伤(AKI)标志物尿L-FABP、CHI3L1的影响。方法:将3~4期CKD患者92例,按照1∶1的比例随机分到治疗组或对照组,即每组46例。对照组给予内科基础治疗;治疗组在对照组基...目的:观察川黄方治疗3~4期慢性肾脏病(CKD)患者的临床疗效,及对急性肾损伤(AKI)标志物尿L-FABP、CHI3L1的影响。方法:将3~4期CKD患者92例,按照1∶1的比例随机分到治疗组或对照组,即每组46例。对照组给予内科基础治疗;治疗组在对照组基础上加服川黄方。疗程为12周。进行临床疗效评价,并观察中医证候积分变化。检测治疗前后肾功能(Scr、BUN、UA、eGFR),24 h U-pro,尿L-FABP、尿CHI3L1变化。结果:(1)治疗组总有效率为73.9%,对照组43.5%,治疗组高于对照组(P<0.05)。(2)用药后,两组患者肾Scr、BUN、UA均有一定程度的降低,eGFR均有一定程度的升高,肾功能得到了改善。治疗组更明显(P<0.01)。两组患者UA差异无统计学意义(P>0.05)。(3)治疗后两组患者24 h U-pro均有降低,治疗组更显著(P<0.01)。(4)两组患者尿L-FABP、CHI3L1治疗前后比较,差异均有统计学意义(P<0.01)。治疗组降低更明显。(5)治疗后,两组患者中医证候积分均显著降低(P<0.01),说明两种治疗方法均有利于改善患者临床症状。结论:川黄方联合内科基础治疗的临床疗效优于单纯内科基础治疗,能更好地促进3~4期CKD患者肾功能恢复、延缓CKD进展,并能显著降低患者尿L-FABP和尿CHI3L1。展开更多
The endoplasmic reticulum plays an extremely important role in the process of cellular protein secretion.The cyclic AMP-responsive element-binding protein 3(CREB3)transcription factor family is closely associated with...The endoplasmic reticulum plays an extremely important role in the process of cellular protein secretion.The cyclic AMP-responsive element-binding protein 3(CREB3)transcription factor family is closely associated with the secretion and transport of proteins within the endoplasmic reticulum.As a member of the CREB3 transcription factor family,cyclic AMP-responsive element-binding protein 3-like protein 2(CREB3L2)stands out as a non-classical sensor within the endoplasmic reticulum.CREB3L2 can detect and regulate endoplasmic reticulum pressure,exert control over the processes of protein transport and secretion,participate in the development of tumor cells,and is also closely linked to the development of certain human tissues and organs.This article aims to review the role of CREB3L2 in tissue development and disease,shedding light on the related mechanisms of CREB3L2 in cancer development.The goal is to provide insights and directions for further analysis of CREB3L2.展开更多
基金Research Fund for Young and Middle-aged Teachers'Basic Research Ability Promotion Project of Guangxi Universities in 2021(No.2021KY0539)。
文摘Objective: To investigate the effect of SLC6A8 on the proliferation of liver cancer cells by regulating mitophagy through BNIP3L. Methods: The expression of the SLC6A8 gene in liver cancer tissues was analyzed using the TCGA database, and its correlation with BNIP3L expression was assessed. RT-PCR and Western blot techniques were employed to detect the expression of SLC6A8 and BNIP3L in human liver cancer Huh-7 and Hep3B cells. Immunofluorescence labeling and CCK-8 assay were used to observe mitochondrial autophagy and cell proliferation rate in SLC6A8-overexpressing Huh-7 and Hep3B cells. Evaluate the proliferation rate of SLC6A8-overexpressing Huh-7 and Hep3B cells after silencing BNIP3L using the CCK-8 detection method. Results: SLC6A8 was significantly overexpressed in liver cancer tissues and positively correlated with BNIP3L expression. Overexpression of SLC6A8 significantly promoted mitochondrial autophagy and proliferation of Huh-7 and Hep3B cells. Additionally, SLC6A8 overexpression significantly enhanced the expression of BNIP3L mRNA and protein. Upon BNIP3L silencing, the proliferative effect of SLC6A8 overexpression on liver cancer cells was reversed. Conclusion: High expression of SLC6A8 in liver cancer tissues is positively correlated with BNIP3L, and overexpression of SLC6A8 promotes mitochondrial autophagy and liver cancer cell proliferation. Silencing BNIP3L can reverses the effect of overexpression of SLC6A8 on liver cancer cell proliferation. This provides new targets and strategies for the treatment of liver cancer.
文摘目的:观察川黄方治疗3~4期慢性肾脏病(CKD)患者的临床疗效,及对急性肾损伤(AKI)标志物尿L-FABP、CHI3L1的影响。方法:将3~4期CKD患者92例,按照1∶1的比例随机分到治疗组或对照组,即每组46例。对照组给予内科基础治疗;治疗组在对照组基础上加服川黄方。疗程为12周。进行临床疗效评价,并观察中医证候积分变化。检测治疗前后肾功能(Scr、BUN、UA、eGFR),24 h U-pro,尿L-FABP、尿CHI3L1变化。结果:(1)治疗组总有效率为73.9%,对照组43.5%,治疗组高于对照组(P<0.05)。(2)用药后,两组患者肾Scr、BUN、UA均有一定程度的降低,eGFR均有一定程度的升高,肾功能得到了改善。治疗组更明显(P<0.01)。两组患者UA差异无统计学意义(P>0.05)。(3)治疗后两组患者24 h U-pro均有降低,治疗组更显著(P<0.01)。(4)两组患者尿L-FABP、CHI3L1治疗前后比较,差异均有统计学意义(P<0.01)。治疗组降低更明显。(5)治疗后,两组患者中医证候积分均显著降低(P<0.01),说明两种治疗方法均有利于改善患者临床症状。结论:川黄方联合内科基础治疗的临床疗效优于单纯内科基础治疗,能更好地促进3~4期CKD患者肾功能恢复、延缓CKD进展,并能显著降低患者尿L-FABP和尿CHI3L1。
基金Hebei Province Medical Science Research Plan Project(20220282)Baoding Science and Technology Plan Project(2141ZF237)。
文摘The endoplasmic reticulum plays an extremely important role in the process of cellular protein secretion.The cyclic AMP-responsive element-binding protein 3(CREB3)transcription factor family is closely associated with the secretion and transport of proteins within the endoplasmic reticulum.As a member of the CREB3 transcription factor family,cyclic AMP-responsive element-binding protein 3-like protein 2(CREB3L2)stands out as a non-classical sensor within the endoplasmic reticulum.CREB3L2 can detect and regulate endoplasmic reticulum pressure,exert control over the processes of protein transport and secretion,participate in the development of tumor cells,and is also closely linked to the development of certain human tissues and organs.This article aims to review the role of CREB3L2 in tissue development and disease,shedding light on the related mechanisms of CREB3L2 in cancer development.The goal is to provide insights and directions for further analysis of CREB3L2.
