通过简并引物和降落PCR方法结合RACE(rapid amplification of c DNA end)技术在香蕉果实c DNA文库中获得2个14-3-3基因,分别命名为Ma14-3-3d和Ma14-3-3h,把Ma14-3-3d和Ma14-3-3h与Ma14-3-3a、Ma14-3-3c、Ma14-3-3e、Ma14-3-3i进行多重...通过简并引物和降落PCR方法结合RACE(rapid amplification of c DNA end)技术在香蕉果实c DNA文库中获得2个14-3-3基因,分别命名为Ma14-3-3d和Ma14-3-3h,把Ma14-3-3d和Ma14-3-3h与Ma14-3-3a、Ma14-3-3c、Ma14-3-3e、Ma14-3-3i进行多重序列比对和同源性比较。结果表明:Ma14-3-3d和Ma14-3-3h与Ma14-3-3a、Ma14-3-3c、Ma14-3-3e、Ma14-3-3i具有较高的同源性,同时具有一定的差异性。遗传进化分析结果表明,Ma14-3-3d与Ma14-3-3a、Ma14-3-3c、Ma14-3-3e、Ma14-3-3i同属于non-ε类14-3-3基因,Ma14-3-3h属于ε类14-3-3基因。RT-PCR分析结果表明,Ma14-3-3d和Ma14-3-3h在香蕉不同器官中差异表达,Ma14-3-3d在香蕉的根、茎、叶、花和果中均有表达,且在茎、叶和花中的表达量高于根和果;Ma14-3-3h在根、花和果中的表达量较高,而在茎和叶中的表达量较低。q RT-PCR分析结果表明,Ma14-3-3d基因的表达明显受乙烯的诱导,而Ma14-3-3h在正常成熟、乙烯处理与1-MCP处理的果实中表达量均很低,与果实成熟的相关性不明显。推测Ma14-3-3d可能与香蕉果实成熟密切相关,可能参与乙烯调控果实成熟过程中的生物合成与信号转导。展开更多
The mixed-cation lead halide perovskites have emerged as a new class of promising light harvesting materials for solar cells. The formamidinium（FA）, methylammonium（MA） and Cs cations are widely studied in the fiel...The mixed-cation lead halide perovskites have emerged as a new class of promising light harvesting materials for solar cells. The formamidinium（FA）, methylammonium（MA） and Cs cations are widely studied in the field of mixed-cation perovskites. Here, we have investigated ethylammonium（EA） as an alternative cation to fabricate a mixed-cation perovskite of MA_（1-x）EA_xPbI_3. We have characterized the materials using the X-ray diffraction（XRD）, scanning electron microscope（SEM）, and UV–vis spectrum. Our results have confirmed the successful incorporation of EA cations into MAPbI_3. Interestingly, the optimal amount of EA to achieve the best performance is quite low. This is different from the FA–MA mixed-cation perovskites although EA and FA have similar radii. In short, the EA–MA mixed-cation perovskite has some material and device properties highly distinguishable from the FA–MA one.展开更多
文摘通过简并引物和降落PCR方法结合RACE(rapid amplification of c DNA end)技术在香蕉果实c DNA文库中获得2个14-3-3基因,分别命名为Ma14-3-3d和Ma14-3-3h,把Ma14-3-3d和Ma14-3-3h与Ma14-3-3a、Ma14-3-3c、Ma14-3-3e、Ma14-3-3i进行多重序列比对和同源性比较。结果表明:Ma14-3-3d和Ma14-3-3h与Ma14-3-3a、Ma14-3-3c、Ma14-3-3e、Ma14-3-3i具有较高的同源性,同时具有一定的差异性。遗传进化分析结果表明,Ma14-3-3d与Ma14-3-3a、Ma14-3-3c、Ma14-3-3e、Ma14-3-3i同属于non-ε类14-3-3基因,Ma14-3-3h属于ε类14-3-3基因。RT-PCR分析结果表明,Ma14-3-3d和Ma14-3-3h在香蕉不同器官中差异表达,Ma14-3-3d在香蕉的根、茎、叶、花和果中均有表达,且在茎、叶和花中的表达量高于根和果;Ma14-3-3h在根、花和果中的表达量较高,而在茎和叶中的表达量较低。q RT-PCR分析结果表明,Ma14-3-3d基因的表达明显受乙烯的诱导,而Ma14-3-3h在正常成熟、乙烯处理与1-MCP处理的果实中表达量均很低,与果实成熟的相关性不明显。推测Ma14-3-3d可能与香蕉果实成熟密切相关,可能参与乙烯调控果实成熟过程中的生物合成与信号转导。
基金the support of the NSFC(Grant 51372151 and21303103)Huoyingdong Grant(151046)
文摘The mixed-cation lead halide perovskites have emerged as a new class of promising light harvesting materials for solar cells. The formamidinium（FA）, methylammonium（MA） and Cs cations are widely studied in the field of mixed-cation perovskites. Here, we have investigated ethylammonium（EA） as an alternative cation to fabricate a mixed-cation perovskite of MA_（1-x）EA_xPbI_3. We have characterized the materials using the X-ray diffraction（XRD）, scanning electron microscope（SEM）, and UV–vis spectrum. Our results have confirmed the successful incorporation of EA cations into MAPbI_3. Interestingly, the optimal amount of EA to achieve the best performance is quite low. This is different from the FA–MA mixed-cation perovskites although EA and FA have similar radii. In short, the EA–MA mixed-cation perovskite has some material and device properties highly distinguishable from the FA–MA one.
