[Objective] The aim was to explore the molecular mechanisms of the A223C polymorphism at 3＇UTR of oxidized low density lipoprotein receptor 1（OLR1）which was closely related to milk fat percentage in cow.[Method]The...[Objective] The aim was to explore the molecular mechanisms of the A223C polymorphism at 3＇UTR of oxidized low density lipoprotein receptor 1（OLR1）which was closely related to milk fat percentage in cow.[Method]The putative microRNA targets located in the 3＇UTR of OLR1 were predicted with the software miRanda（1.0）which could carry the microRNA database of cow.[Result]Total 16 microRNA targets were found.The A223C SNP was located in complementary region of bta-miR-370 target sequence.[Conclusion]OLR1 3＇UTR A→C mutation had led to the disappearing of bta-miR-370 target,which had provided basis for the further research on the molecular mechanisms of the A223C polymorphism at 3＇UTR of OLR1 gene which was closely related to milk fat percentage in cow.展开更多
钙蛋白酶抑制蛋白(Calpastatin,CAST)专一抑制钙蛋白酶(Calpain,CAPN)活性,参与调节牛肉的嫩化及肌内蛋白的水解,其编码基因是肉质性状的主要候选基因。本研究根据牛CAST基因5'UTR区(GenBank No.AY834771)和3'UTR区(Gen Bank No...钙蛋白酶抑制蛋白(Calpastatin,CAST)专一抑制钙蛋白酶(Calpain,CAPN)活性,参与调节牛肉的嫩化及肌内蛋白的水解,其编码基因是肉质性状的主要候选基因。本研究根据牛CAST基因5'UTR区(GenBank No.AY834771)和3'UTR区(Gen Bank No.DQ991097)序列设计两对引物,用PCR-SSCP方法对鲁西黄牛和渤海黑牛共305个样本进行了单核苷酸多态性分析。结果显示,CAST基因5'UTR和3'UTR区存在PCR-SSCP多态,在5'UTR区存在6437(C/T)、6477(G/A)和6614(G/T)3个SNP位点,表现为AA、AB、BB、BC和AD 5种基因型;在3'UTR区存在359(T/C)和366(A/G)2个SNP位点,表现为EE、EF、FF和EG 4种基因型。经检验表明,鲁西黄牛和渤海黑牛均处于非Hardy-Weinberg平衡状态;群体遗传多态性分析表明这两种牛的多态信息含量均为中度多态。展开更多
基金Supported by Key Project in the National Science & Technology Pillar Program during the Eleventh Five-Year Plan Period(No.2008BADB2B04-8-4)Natural Science Foundation of Hebei Province(No.C2008001308 and C2007000743)~~
文摘[Objective] The aim was to explore the molecular mechanisms of the A223C polymorphism at 3＇UTR of oxidized low density lipoprotein receptor 1（OLR1）which was closely related to milk fat percentage in cow.[Method]The putative microRNA targets located in the 3＇UTR of OLR1 were predicted with the software miRanda（1.0）which could carry the microRNA database of cow.[Result]Total 16 microRNA targets were found.The A223C SNP was located in complementary region of bta-miR-370 target sequence.[Conclusion]OLR1 3＇UTR A→C mutation had led to the disappearing of bta-miR-370 target,which had provided basis for the further research on the molecular mechanisms of the A223C polymorphism at 3＇UTR of OLR1 gene which was closely related to milk fat percentage in cow.
