Baoziwan-Majiashan Area of Jiyuan Oilfield Analysis of Reservoir Characteristics and Main Control Factors in Long 4 5 Section

Based on the sheet, scanning electron microscope and high pressure mercury analysis method, this paper takes Jiyuan oilfield-Ma Jia mountain district 4 5 sandstone reservoir as the research object, from the reservoir petrology, pore type and porosity, permeability, the system analyzed the reservoir characteristics and its control factors. The results show that the sandstone in the 4 5 section of Baoziwan-Majiashan area of Jiyuan oilfield is fine in size and high in filling content. The pore types were dominated by intergranular pores and dissolved pores, with a low face rate. The reservoir property is relatively poor, with mean porosity of 11.11% and mean permeability of 1.16 × 10−3 µm2. In the low porous, low otonic background, the development of relatively high pore hypertonic areas. Compaction and cementation should play a destructive role in reservoir properties, and dissolution should play a positive role in reservoir properties. Compaction adjusts the migration of clay minerals and miscellaneous bases in the original sediment in the study area, greatly reducing the porosity and permeability of the reservoir;the development of the cement cement, carbonate cementation and some quartz secondary compounds reduces the storage space;the dissolution effect, especially the secondary dissolution pores of the reservoir, which obviously improves the properties of the reservoir.

Platelet factor 4 induces bone loss by inhibiting the integrinα5-FAK-ERK pathway

Background:The effect of platelet factor 4(PF4)on bone marrow mesenchymal stem cells(BMMSCs)and osteoporosis is poorly understood.Therefore,this study aimed to evaluate the effects of PF4-triggered bone destruction in mice and determine the underlying mechanism.Methods:First,in vitro cell proliferation and cell cycle of BMMSCs were assessed using a CCK8 assay and flow cytometry,respectively.Osteogenic differentiation was confirmed using staining and quantification of alkaline phosphatase and Alizarin Red S.Next,an osteoporotic mouse model was established by performing bilateral ovariectomy(OVX).Furthermore,the PF4 concentrations were obtained using enzymelinked immunosorbent assay.The bone microarchitecture of the femur was evaluated using microCT and histological analyses.Finally,the key regulators of osteogenesis and pathways were investigated using quantitative real-time polymerase chain reaction and Western blotting.Results:Human PF4 widely and moderately decreased the cell proliferation and osteogenic differentiation ability of BMMSCs.Furthermore,the levels of PF4 in the serum and bone marrow were generally increased,whereas bone microarchitecture deteriorated due to OVX.Moreover,in vivo mouse PF4 supplementation triggered bone deterioration of the femur.In addition,several key regulators of osteogenesis were downregulated,and the integrinα5-focal adhesion kinase-extracellular signalregulated kinase(ITGA5-FAK-ERK)pathway was inhibited due to PF4 supplementation.Conclusions:PF4 may be attributed to OVX-i nduced bone loss triggered by the suppression of bone formation in vivo and alleviate BMMSC osteogenic differentiation by inhibiting the ITGA5-FAK-ERK pathway.

Wnt通路拮抗基因SFRP1 SFRP2 SFRP4 SFRP5甲基化状态与肾透明细胞癌关系的研究

目的:研究SFRPs家族中SFRP1、SFRP2、SFRP4、SFRP5基因启动子区甲基化状况,探讨基因的甲基化与肾透明细胞癌发生发展的关系。方法:采用甲基化特异性PCR(methylation specific PCR,MSP)方法检测66例肾透明细胞癌及30例癌旁组织中SFRP1、SFRP2、SFRP4、SFRP5基因启动子区甲基化状态及其与临床病理学资料之间的关系。结果:肾透明细胞癌组织中SFRP1、SFRP2、SFRP4、SFRP5基因甲基化率分别为77.3%(51/66)、72.7%(48/66)、59.1%(39/66)、69.7%(46/66),均显著高于相应的癌旁组织,结果有统计学意义(P<0.05)。与临床病理学资料相联系,肾透明细胞癌组织中SFRP1、SFRP5基因甲基化与肿瘤TNM分期相关;SFRP4基因甲基化与肿瘤的病理学分级相关(P<0.05)。结论:SFRP1、SFRP2、SFRP4、SFRP5基因的甲基化均可能参与肾透明细胞癌的发生。SFRP1、SFRP5基因甲基化可能与肾透明细胞癌的发展,浸润和转移有关。SFRP4基因甲基化可能与肾透明细胞癌的恶性行为有关。

黄牛与牦牛ANGPTL4 exon5多态性与生长性状的相关性

以5个地方黄牛及青海牦牛为研究对象,利用PCR-SSCP和DNA测序技术对367头黄牛与牦牛的类血管生长因子4基因的第5外显子(ANGPTL4_exon5)序列进行SNP检测,并分析该SNP与黄牛与牦牛生长性状的相关性。结果发现1个新的SNP多态位点(NC_007305:g.C4899T),并检测出CC、TT和CT3种基因型。黄牛和牦牛不同基因型与生长发育性状的相关分析显示,黄牛TT基因型个体的体质量(399.096±32.946)kg、体斜长(147.447±4.456)cm、胸围(175.188±5.814)cm和腹围(204.657±7.113)cm,显著大于CT基因型个体,依次为(275.636±51.266)kg、(126.470±6.933)cm、(154.031±9.047)cm、(178.911±11.069)cm,其余各项指标基因型间差异不显著。因此,该位点有可能作为黄牛生长性状辅助选择的标记之一。青海牦牛群体中没有发现与该基因座多态显著相关的性状。

儿童异位性皮炎血清IL-4 IL-5及IgE水平的测定

目的 为了探讨儿童异位性皮炎的发病机制与细胞因子的关系 ,了解异位性皮炎患儿血清IL 4 ,IL 5及IgE水平。 方法 运用ELISA方法测定 2 0例异位性皮炎及 2 0例正常儿童血清IL 4 ,IL 5及IgE水平。 结果患病组IL 4 ,IL 5均高于正常对照组 ,差异有显著性意义 (P <0 .0 1) ,患病组IgE水平高于对照组 (P <0 .0 1)。结论异位性皮炎患儿外周血TH2类淋巴细胞因子增高。

哮喘大鼠血单个核细胞中STAT5b mRNA和IL-4 mRNA的表达及布地奈德的影响

目的研究哮喘大鼠血单个核细胞中信号转导和转录激活因子STAT5bmRNA和IL-4mRNA的转录表达及布地奈德(BUD)对其表达的影响。方法30只体重为140￣200g清洁级SD雌性大鼠,随机分为3组:哮喘组(A组)、BUD组(B组)、正常对照组(C组)。对血中嗜酸性粒细胞(EOS)计数;应用双抗体夹心ELISA测定血浆中IL-4和IFN-γ水平;采用SYBRGREENI荧光定量PCR法测定STAT5bmRNA和IL-4mRNA的相对含量。结果(1)A组血中单个核细胞STAT5bmRNA和IL-4mRNA的表达均高于B组和C组(P<0.01);B组与C组之间差异无统计学意义(P>0.05)(。2)STAT5bmRNA与IL-4mRNA的表达呈正相关(P<0.01),且两者的表达分别与血浆中的IL-4水平、EOS绝对值呈正相关(P<0.01),与血浆中IFN-γ水平呈负相关(P<0.01)。结论哮喘大鼠血中单个核细胞STAT5bmRNA和IL-4mRNA的表达增强且呈正相关;布地奈德可以下调STAT5bmRNA和IL-4mR-NA的表达,可能是其抑制哮喘气道炎症形成的重要机制之一。

DNA修复基因ERCC4 rs6498486、ERCC5 rs751402多态性与唾液腺肿瘤发病风险的关系

目的:探讨DNA修复基因ERCC4、ERCC5多态性与唾液腺肿瘤发病风险的关系。方法:采用病例-对照研究方法,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术对133例经组织学确诊的唾液腺肿瘤病例和142例按年龄、性别匹配的健康对照组的ERCC4 rs6498486与ERCC5 rs751402 2个多态基因型进行检测,应用SPSS18.0软件包中的多因素Logistic回归分析进行基因多态性与唾液腺肿瘤发病风险关系评估。结果:与(CC+CT)型相比,ERCC5 rs751402多态纯合TT型携带者唾液腺肿瘤发病风险降低(OR=0.45,95%CI:0.21,0.98),差异具有显著性(P<0.05)。唾液腺肿瘤病例组与对照组间ERCC4 rs6498486多态基因型频率分布无显著差异(P>0.05)。结论:携带ERCC5 rs751402多态TT型个体唾液腺肿瘤的发病风险可能降低。

T_4 DNA连接酶介导的5’RACE法克隆大鼠Nor 1全长cDNA

目的:克隆75AEST全长cDNA序列。方法:抽提在25mmol/L KCl条件下培养7d的大鼠小脑颗粒神经元的总RNA后,用DNA连接酶介导的5’RACE法克隆75A表达序列标签(EST)cDNA5’端未知序列,其产物亚克隆到pGEM-Teasy后进行序列测定以及同源性分性。结果:首轮5’RACE扩增出2.5kb序列后,75A EST鉴定为神经来源孤儿受体1(Nor1)基因的部分序列;再经过二次轮5’RACE,我们首次克隆Nor-1的全长cDNA序列。结论:T_4DNA连接酶介导的5’RACE是一种效率较高的克隆mRNA5’端未知序列的好方法,为进一步研究Nor1在小脑颗粒神经元存活或分化过程中的作用奠定基础。

鼻炎宁颗粒对过敏性鼻炎大鼠IL-4 IL-5和TNF-α含量的影响

目的:观察鼻炎宁颗粒对过敏性鼻炎大鼠血浆IL-4、IL-5和TNF-α含量的影响,揭示鼻炎宁颗粒治疗过敏性鼻炎的部分现代生物学基础。方法:将80只SD雄性大鼠按体重随机分成正常组、过敏性鼻炎组、鼻炎宁颗粒组、息斯敏组,每组20只。采用卵白蛋白全身致敏与局部攻击方法制作过敏性鼻炎大鼠模型,观察鼻炎宁颗粒治疗后血浆IL-4、IL-5和TNF-α含量的变化,结果进行统计学分析。结果:模型组血浆IL-4、IL-5和TNF-α的含量明显升高,鼻炎宁颗粒治疗组血浆IL-4、IL-5和TNF-α的含量明显降低。结论:鼻炎宁颗粒治疗过敏性鼻炎的作用机制之一可能是通过降低IL-4、IL-5和TNF-α的含量,从而减轻鼻黏膜变应性炎症,减轻过敏性鼻炎的症状或减缓过敏性鼻炎的发作,以达到治疗过敏性鼻炎的目的。

Protective efficacy of an H5/H7 trivalent inactivated vaccine(H5-Re13,H5-Re14, and H7-Re4 strains) in chickens, ducks, and geese against newly detected H5N1, H5N6, H5N8, and H7N9 viruses

Some H5 viruses isolated in poultry or wild birds between 2020 and 2021 were found to be antigenically different from the vaccine strains(H5-Re11 and H5-Re12) used in China. In this study, we generated three new recombinant vaccine seed viruses by using reverse genetics and used them for vaccine production. The vaccine strain H5-Re13 contains the hemagglutinin(HA) and neuraminidase(NA) genes of an H5 N6 virus that bears the clade 2.3.4.4 h HA gene, H5-Re14 contains the HA and NA genes of an H5 N8 virus that bears the clade 2.3.4.4 b HA gene, and H7-Re4 contains the HA and NA genes of H7 N9 virus detected in 2021. We evaluated the protective efficacy of the novel H5/H7 trivalent inactivated vaccine in chickens, ducks, and geese. The inactivated vaccine was immunogenic and induced substantial antibody responses in the birds tested. Three weeks after vaccination, chickens were challenged with five different viruses detected in 2020 and 2021: three viruses(an H5 N1 virus, an H5 N6 virus, and an H5 N8 virus) bearing the clade 2.3.4.4 b HA gene, an H5 N6 virus bearing the clade 2.3.4.4 h HA gene, and an H7 N9 virus. All of the control birds shed high titers of virus and died within 4 days post-challenge, whereas the vaccinated chickens were completely protected from these viruses. Similar protective efficacy against H5 viruses bearing the clade 2.3.4.4 h or 2.3.4.4 b HA gene was observed in ducks and geese. Our study indicates that the newly updated H5/H7 vaccine can provide solid protection against the H5 and H7 N9 viruses that are currently circulating in nature.

水中2-氨基-3-氰基-4-芳基-7,7-二甲基-5-氧代-4 H-5,6,7,8-四氢苯并[b]吡喃的洁净合成

在水溶剂中在三乙基苄基氯化铵 (TEBA)存在下 ,取代肉桂腈与 5 ,5 二甲基 1,3 环己酮反应为 2 氨基 3 氰基 4 芳基 7,7 二甲基 5 氧代 4H 5 ,6,7,8 四氢苯并 [b]吡喃提供了一种快速、方便、高效和洁净的合成方法 .

顺(反)-2,4,7-三芳基5-氧代-4 H-5,6,7,8-四氢苯并吡喃衍生物的合成

在酸或碱性条件下 ,5 ,5 二甲基 1,3 环己二酮和 5 芳基 1,3 环己二酮与查尔酮首先进行Michael加成反应 ,进而环合为 7,7 二甲基 2 ,4 二芳基 5 氧代 4H 5 ,6,7,8 四氢苯并吡喃衍生物和含有两个手性原子的顺 和反 2 ,4,7 三芳基 5 氧代 4H 5 ,6,7,8 四氢苯并吡喃衍生物 .讨论了反应机理并且通过1HNMR光谱和NOE差谱确定其产物构型 .所合成新的多氢苯并吡喃衍生物的分子结构均经红外光谱、核磁共振光谱和元素分析予以证实 .

新型配体 9′-( 4 ,5-二烷基硫-1,3-二硫杂环戊烯-2-叉) 4′,5′-二氮杂芴的铜 (I)配合物的合成及光谱研究(英文)

首次用Cu(CH3CN) 4 ClO4与新型配体 9′ (4,5 二烷基硫 1,3 二硫杂环戊烯 2 叉 ) 4′ ,5′ 二氮杂芴合成了两种Cu(I)配合物 ,并对配合物进行了元素分析和光谱的测定 .

地佐辛术后静脉镇痛对髋关节置换术患者血浆白三烯B4 5-羟色胺及去甲肾上腺素的影响

目的：观察地佐辛术后静脉镇痛对髋关节置换术患者血浆白三烯B4（LTB4）、5-羟色胺（5-HT）和去甲肾上腺素（ NE）的影响。方法择期全麻下髋关节置换术患者60例随机均分为地佐辛组（ D组）、吗啡组（ M组）和对照组（ N组）。 D组：地佐辛0．8 mg/kg加0．9％氯化钠注射液稀释到100 mL，给药速率为2 mL/h持续泵入，负荷剂量为0．15 mg/kg；M组：吗啡0．5 mg/kg加0．9％氯化钠注射液稀释到100 mL，给药速率2 mL/h持续泵入，负荷剂量为0．05 mg/kg；N组：在患者需要时给予肌注吗啡10 mg。分别于麻醉诱导前（ T0）、术后12小时（ T1）、24小时（ T2）、36小时（ T3）及48小时（ T4）采集肘静脉血3 mL，采用酶联免疫吸附测定法（ ELISA）测定血浆LTB4、5-HT和NE的浓度。观察并记录T1、T2、T3及T4视觉模拟评分（ VAS）得分。结果 D组和M组T1～T4时LTB4、5-HT和NE明显低于N组（P＜0．05）；D、M组T1、T2时VAS评分明显低于N组。结论地佐辛与吗啡一样，术后静脉镇痛能抑制LTB4、5-HT和NE的升高。

Anti-Anxiety Effects of Prelimbic 5-HT4 Receptors in the Rat Model of Parkinson’s Disease: Evidence from Behavioral and Electrophysiological Study

Objective:Clinical and laboratory studies have demonstrated that prelimbic(PrL)and serotonin-4(5-HT4)receptors may have the key role in regulating anxiety.However,the pathophysiology of anxiety in Parkinson’s disease(PD)remains obscure.In this research,the effects of PrL 5-HT4 receptors on anti-anxiety behaviors in hemiparkinsonian rats were investigated.Methods:PD model rats were used as the research subjects,starting with behavioral changes,from the point of view of electrophysiology,the regulatory effect of PrL 5-HT4 receptors on PD-related anxiety and the possible mechanism were explored.Results:Anxiety-like behaviors were induced via MFB lesion in rats.Intra-PrL injection of 5-HT4 receptors agonist RS67333 induced anti-anxiety effects in both sham and PD group.In the sham group,PrL administration of 5-HT4 receptors antagonist SB204070 produce anti-anxiety effects,but in the PD group,the expression of anxiety-like behavior was increased.Compared to the sham group,the effective dose of the behavioral effects of the two drugs in the PD group was obviously higher.Electrophysiological data suggested that PrL administration of RS67333(SB204070)increased(decreased)the firing activities ofγ-aminobutyric acid(GABA)neurons in both groups.Compared with rats in sham group,lesioned rats had a shorter duration of the excitation(inhibition)effects on firing activities of GABA neurons.Conclusion:PrL 5-HT4 receptors regulate anxiety behaviors in PD rats,and its mechanism may be related to the down-regulation of expression or function of PrL 5-HT4 receptors in PD.

−675 4G/5G and −844 G/A of Plasminogne Activator Inhibitor-1 (Pai-1) Gene Polymorphisms and Type 2 DiabetesMellitus in Tunisia: Case-Control Study

Background: The plasminogen activator inhibitor-1 (PAI-1) is a puissant antifibrinolytic factor;plasma PAI-1 level is high in type 2 diabetes. 4G/5G polymorphism of PAI-1 gene is a major genetic determinant of plasma PAI-1 levels, with 4G carriers having high PAI-1 level than 5G, theses pose the question about relation T2 patients and those polymorphisms. The aim of this study was to determine the relationship between the polymorphisms &minus;675 4G/5G and &minus;844 G/A of PAI-1 gene and type 2 diabetes mellitus. Methods: A case control study of 491 diabetic and 400 healthy controls. Genotyping of the polymorphism &minus;675 4G/5G was done by PCR-ASA (polymerase chain reaction, allele specific amplification), and the polymorphism &minus;844 G/A was done with PCR-RFLP (restriction fragment length polymorphism), the allelic frequency is calculated with hardy-Weinberg law, the statistic analysis was done by SPSS version 10. Results: Higher frequencies of The genotypes 4G/4G (p = 0.01) and 4G/5G of polymorphism &minus;675 4G/5G were seen in diabetic (p = 0.05) and higher frequencies of 5G/5G was seen in controls (p &minus;844 G/A was seen in diabetics and G/G was seen in controls (p = 0.01). Conclusion: Our study found association between 4G allele of &minus;675 4G/5G and A allele of &minus;844 G/A of PAI-1 gene and having type 2 diabetes mellitus in Tunisian population.

Methylation of GATA-4 and GATA-5 and development of sporadic gastric carcinomas

AIM:To understand the implication of GATA-4 and GATA-5 methylation in gastric carcinogenesis.METHODS: Methylation status of GATA-4 and GATA-5 CpG islands in human gastric mucosa samples, including normal gastric biopsies from 45 outpatients, gastric dysplasia [low-grade gastric intraepithelial neoplasia (GIN), n = 30; indefinite, n = 77], and 80 paired spo- radic gastric carcinomas (SGC) as well as the adjacent non-neoplastic gastric tissues was analyzed by methylation specific polymerase chain reaction (MSP) and confirmed by denatured high performance liquid chromatography (DHPLC). Immunohistochemical staining was used to detect protein expression. The correlation between GATA-4 and GATA-5 methylation and clinicopathological characteristics of patients including Helicobacter pylori (H. pylori) infection was analyzed.RESULTS:GATA-4 and GATA-5 methylation was frequently observed in SGCs (53.8% and 61.3%, respectively) and their corresponding normal tissues (41.3% and 46.3%) by MSP. The result of MSP was consistent with that of DHPLC. Loss of both GATA-4 and GATA-5 proteins was associated with their methylation in SGCs (P = 0.01). Moreover, a high frequency of GATA-4 and GATA-5 methylation was found in both gastric low-grade GIN (57.1% and 69.0%) and indefinite for dysplasia (42.9% and 46.7%), respectively. However, GATA-4 and GATA-5 methylation was detected only in 4/32 (12.5%) and 3/39 (7.7%) of normal gastric biopsies. GATA-4 methylation in both normal gastric mucosa and low-grade GIN was also significantly associated with H. pylori infection (P=0.023 and 0.027, two-sides).CONCLUSION: Epigenetic inactivation of GATA-4 (and GATA-5) by methylation of CpG islands is an early freuent event during gastric carcinogenesis and is significantly correlated with H. pylori infection.

赝二元系KBe_2F_5-KAlF_4 和 KBe_2F_5-K_3AlF_6的相图研究

采用溶液反应法制备试样,用DTA和XRD方法研究了KBe2F5-KAlF4和KBe2F5-K3AlF6两个赝二元体系。结果表明:KBe2F5-KAlF4是一简单低共熔体系,共晶点组成含KBe2F580mol%,温度335℃。KBe2F5-K3AlF6体系中生成了两个中间化合物2K3AlF6·KBe2F5和K3AlF6·KBe2F5。相图中有一共晶点,组成为85mol%KBe2F5,温度336℃。2K3AlF6·KBe2F5和K3AlF6·KBe2F5都是固液异组成化合物,分别在448℃和378℃时发生分解。X-射线衍射分析证明它们均属正交晶系,晶胞参数分别为a=10.70±0.01,b=10.36±0.01,c=5.85±0.02( )和a=10.499±0.003,b=9.685±0.005,c=7.554±0.005( )。

鄂尔多斯盆地胡154 区块长4+5 段孔隙结构定量表征及分类

鄂尔多斯盆地胡尖山油田胡154区块三叠系延长组长4+5段属于低孔低渗储集层,孔隙结构相关研究较少,缺乏详细定量化分析,导致胡154区块产量下降较快,水驱控制程度不高。为定量表征研究区目的层孔隙结构,利用岩心、薄片、铸体薄片、扫描电镜、高压压汞及相渗实验资料,对孔隙结构进行定量表征及分类,结合测井曲线,对不同类型孔隙结构储集层在平面上的分布进行研究。结果表明,研究区长4+5段孔隙类型以剩余粒间孔为主,喉道类型主要为片状和弯片状。孔隙结构划分为3类:Ⅰ类孔隙结构分形维数分布于2.57~2.61,排驱压力平均为1.62 MPa,日产油量2 t以上;Ⅱ类孔隙结构分形维数分布于2.61~2.66,排驱压力平均为2.61 MPa,日产油量1~2 t;Ⅲ类孔隙结构分形维数分布于2.66~2.71,排驱压力平均为3.52 MPa,日产油量小于1 t。具有Ⅱ类孔隙结构和Ⅲ类孔隙结构的储集层在平面上分布较广,长(4+5)2孔隙结构相对好于长(4+5)1。