The structure of N -(4-chlorobenzyl)-2,3-methenedioxy-9-acetoxyl-10-methyloxyl-7,8,13,13a-tetrahydro-8H-dibenzo-quinolizine chloride has been studied with IR, MS, HRMS and NMR( 1HNMR, 13 CNMR, COSY, HETCOR).
目的探讨使用单克隆抗-CD47(简称抗-CD47单抗)对患者输血前检测干扰的解决策略。方法以抗-CD47单抗类药物临床试验的输血病例为研究对象,检索PUBMED、MEDLINE、web of science、万方数据知识服务平台及中国知网数据库中检索国内外病例报...目的探讨使用单克隆抗-CD47(简称抗-CD47单抗)对患者输血前检测干扰的解决策略。方法以抗-CD47单抗类药物临床试验的输血病例为研究对象,检索PUBMED、MEDLINE、web of science、万方数据知识服务平台及中国知网数据库中检索国内外病例报告,从中提取病例的输血前检测信息,分析抗-CD47单抗干扰输血前检测的主要方面及解决策略。结果共检索到26例使用抗-CD47单抗药后进行输血前检测的病例,排除无效病例,共纳入16例病例(均使用的抗-CD47单抗药均是Hu5F9-G4)。使用Hu5F9-G4治疗后,输血前检测发现ABO血型鉴定正反不符率达69%,抗体筛查全部凝集,血小板抗体筛查也100%被干扰。这些说明Hu5F9-G4严重影响输血前检测。进一步分析病例中采用的排除抗-CD47干扰的方法及其优缺点。结论根据现有临床病例中抗-CD47单抗干扰输血前检测的特点,分析了排除抗-CD47对输血前检测策略的优缺点,为输血的实验室检测提供参考。展开更多
Two new indole alkaloids, 4-oxo-4,12-dihydroindolo[2,3-a]quinolizine-3-carbaldehyde (1) and 1,6,7-trihydro-indolo- [2,3-a]furan[3,4-g]quinolizine-3,4(13H)-dione (2), were isolated from Nauclea officinalis. Their struc...Two new indole alkaloids, 4-oxo-4,12-dihydroindolo[2,3-a]quinolizine-3-carbaldehyde (1) and 1,6,7-trihydro-indolo- [2,3-a]furan[3,4-g]quinolizine-3,4(13H)-dione (2), were isolated from Nauclea officinalis. Their structures were determined on the basis of 1D and 2D NMR spectral data.展开更多
Lithium sulfur(Li-S)batteries have been regarded as a promising next-generation energy storage system with high theoretical specific capacity and energy density,but still facing challenges.In order to make Li-S batter...Lithium sulfur(Li-S)batteries have been regarded as a promising next-generation energy storage system with high theoretical specific capacity and energy density,but still facing challenges.In order to make Li-S batteries more competitive,combination of trapping sites and electrocatalytic properties for polysulfides is an effective way to improve the battery performance.In this study,we prepare a type of multifunctional V3S4-nanowire/graphene composites(V3S4-G)by uniformly dispersing V3S4 nanowires on the graphene substrate.This structure contributes to the sufficient exposure of multifunctional V3S4 active sites which can anchor polysulfides and accelerate reaction kinetics.Thus,the Li-S batteries based on the multifunctional V3S4-G sulfur cathode deliver a stable cycling performance and good rate capability.Even at sulfur loading of 3 mg cm^−2,the V3S4-G sulfur cathode possesses a low capacity decay rate of 0.186%per cycle at 0.5 C.展开更多
Herpes simplex virus type 1(HSV-1)causes lifelong infections worldwide,and currently there is no efficient cure or vaccine.HSV-1-derived tools,such as neuronal circuit tracers and oncolytic viruses,have been used exte...Herpes simplex virus type 1(HSV-1)causes lifelong infections worldwide,and currently there is no efficient cure or vaccine.HSV-1-derived tools,such as neuronal circuit tracers and oncolytic viruses,have been used exten-sively;however,further genetic engineering of HSV-1 is hindered by its complex genome structure.In the present study,we designed and constructed a synthetic platform for HSV-1 based on H129-G4.The complete genome was constructed from 10 fragments through 3 rounds of synthesis using transformation-associated recombination(TAR)in yeast,and was named H129-Syn-G2.The H129-Syn-G2 genome contained two copies of the gfp gene and was transfected into cells to rescue the virus.According to growth curve assay and electron microscopy results,the synthetic viruses exhibited more optimized growth properties and similar morphogenesis compared to the parental virus.This synthetic platform will facilitate further manipulation of the HSV-1 genome for the devel-opment of neuronal circuit tracers,oncolytic viruses,and vaccines.展开更多
文摘The structure of N -(4-chlorobenzyl)-2,3-methenedioxy-9-acetoxyl-10-methyloxyl-7,8,13,13a-tetrahydro-8H-dibenzo-quinolizine chloride has been studied with IR, MS, HRMS and NMR( 1HNMR, 13 CNMR, COSY, HETCOR).
文摘目的探讨使用单克隆抗-CD47(简称抗-CD47单抗)对患者输血前检测干扰的解决策略。方法以抗-CD47单抗类药物临床试验的输血病例为研究对象,检索PUBMED、MEDLINE、web of science、万方数据知识服务平台及中国知网数据库中检索国内外病例报告,从中提取病例的输血前检测信息,分析抗-CD47单抗干扰输血前检测的主要方面及解决策略。结果共检索到26例使用抗-CD47单抗药后进行输血前检测的病例,排除无效病例,共纳入16例病例(均使用的抗-CD47单抗药均是Hu5F9-G4)。使用Hu5F9-G4治疗后,输血前检测发现ABO血型鉴定正反不符率达69%,抗体筛查全部凝集,血小板抗体筛查也100%被干扰。这些说明Hu5F9-G4严重影响输血前检测。进一步分析病例中采用的排除抗-CD47干扰的方法及其优缺点。结论根据现有临床病例中抗-CD47单抗干扰输血前检测的特点,分析了排除抗-CD47对输血前检测策略的优缺点,为输血的实验室检测提供参考。
文摘Two new indole alkaloids, 4-oxo-4,12-dihydroindolo[2,3-a]quinolizine-3-carbaldehyde (1) and 1,6,7-trihydro-indolo- [2,3-a]furan[3,4-g]quinolizine-3,4(13H)-dione (2), were isolated from Nauclea officinalis. Their structures were determined on the basis of 1D and 2D NMR spectral data.
基金We acknowledge the financial support from the National Key R&D Program of China(2016YFA0200102 and 2017YFA0206301)the National Natural Science Foundation of China(51631001,51590882,51672010 and 81421004).
文摘Lithium sulfur(Li-S)batteries have been regarded as a promising next-generation energy storage system with high theoretical specific capacity and energy density,but still facing challenges.In order to make Li-S batteries more competitive,combination of trapping sites and electrocatalytic properties for polysulfides is an effective way to improve the battery performance.In this study,we prepare a type of multifunctional V3S4-nanowire/graphene composites(V3S4-G)by uniformly dispersing V3S4 nanowires on the graphene substrate.This structure contributes to the sufficient exposure of multifunctional V3S4 active sites which can anchor polysulfides and accelerate reaction kinetics.Thus,the Li-S batteries based on the multifunctional V3S4-G sulfur cathode deliver a stable cycling performance and good rate capability.Even at sulfur loading of 3 mg cm^−2,the V3S4-G sulfur cathode possesses a low capacity decay rate of 0.186%per cycle at 0.5 C.
基金Wuhan Institute of Virology for financial support for the research(grant no.EISA020201).
文摘Herpes simplex virus type 1(HSV-1)causes lifelong infections worldwide,and currently there is no efficient cure or vaccine.HSV-1-derived tools,such as neuronal circuit tracers and oncolytic viruses,have been used exten-sively;however,further genetic engineering of HSV-1 is hindered by its complex genome structure.In the present study,we designed and constructed a synthetic platform for HSV-1 based on H129-G4.The complete genome was constructed from 10 fragments through 3 rounds of synthesis using transformation-associated recombination(TAR)in yeast,and was named H129-Syn-G2.The H129-Syn-G2 genome contained two copies of the gfp gene and was transfected into cells to rescue the virus.According to growth curve assay and electron microscopy results,the synthetic viruses exhibited more optimized growth properties and similar morphogenesis compared to the parental virus.This synthetic platform will facilitate further manipulation of the HSV-1 genome for the devel-opment of neuronal circuit tracers,oncolytic viruses,and vaccines.
