Objective： To investigate the effects of synthetic retinoid CD437 and acitretin on cell proliferation, apoptosis, cycle arrest and Bax/ Bcl-2 protein expression of melanoma A375 cell, Methods：MTT assay was used to d...Objective： To investigate the effects of synthetic retinoid CD437 and acitretin on cell proliferation, apoptosis, cycle arrest and Bax/ Bcl-2 protein expression of melanoma A375 cell, Methods：MTT assay was used to determine the anti-proliferative effects of CD437 and acitretin on melanoma A375 cell, Flow cytometry was performed to investigate the influence of CD437 and acitretin on cell cycle and cell apoptosis. SABC immunocytochemistry was employed for detection of Bax/bcl-2 protein expressions. Results：10^-5 mol/L CD437 was more effective than acitretin in inhibiting proliferation and inducing apoptosis of A375 cell after 24 h treatment, growth inhibiting ratio and apoptosis ratio（58.6%vs43.25% and 28.03%vs17.13%, P 〈 0.05 respectively）. CD437 promoted G0/G1 arrest in melanoma A375 cell, however acitretin could not. CD437 and acitretin could up-regulate the expression of Bax protein and downregulate the expression of bcl-2 protein（P 〈 0.05）. Conclusion：CD437 is more effective than acitretin in inhibiting proliferation and inducing apoptosis and cycle arrest on A375 cell, CD437 may have more potentialities than acitretin for subsidiary treatment of melanoma. Mitochondrial apoptosis pathway is partially involved in two drugs inducing apoptosis on A375 cell.展开更多
Flowing with the reform of the hot water heating method in China, heat meter will enter into households in the near future. A portable ultrasonic heat meter is designed in this paper. The meter uses chip microprocesso...Flowing with the reform of the hot water heating method in China, heat meter will enter into households in the near future. A portable ultrasonic heat meter is designed in this paper. The meter uses chip microprocessor MSP430F437 as the data process core, and uses ultrasonic flow sensor to measure flow rate of the hot water, and capture input and output temperatures of the hot water using the thermal resistance sensor Ptl000, and then household energy consumption is calculated via temperature difference between input temperature and output temperature of the hot water multiplied by volume of hot water that is calculated though flow rate integration of hot water. In order to test the performance of the proposed heat meter, experiments is carried out. Both the temperature and flow measurement results satisfy the requirements of accuracy and the heat meter is effective in the heat measurement.展开更多
基金supported by the National Natural Science Foundation of China(No.30371295).
文摘Objective： To investigate the effects of synthetic retinoid CD437 and acitretin on cell proliferation, apoptosis, cycle arrest and Bax/ Bcl-2 protein expression of melanoma A375 cell, Methods：MTT assay was used to determine the anti-proliferative effects of CD437 and acitretin on melanoma A375 cell, Flow cytometry was performed to investigate the influence of CD437 and acitretin on cell cycle and cell apoptosis. SABC immunocytochemistry was employed for detection of Bax/bcl-2 protein expressions. Results：10^-5 mol/L CD437 was more effective than acitretin in inhibiting proliferation and inducing apoptosis of A375 cell after 24 h treatment, growth inhibiting ratio and apoptosis ratio（58.6%vs43.25% and 28.03%vs17.13%, P 〈 0.05 respectively）. CD437 promoted G0/G1 arrest in melanoma A375 cell, however acitretin could not. CD437 and acitretin could up-regulate the expression of Bax protein and downregulate the expression of bcl-2 protein（P 〈 0.05）. Conclusion：CD437 is more effective than acitretin in inhibiting proliferation and inducing apoptosis and cycle arrest on A375 cell, CD437 may have more potentialities than acitretin for subsidiary treatment of melanoma. Mitochondrial apoptosis pathway is partially involved in two drugs inducing apoptosis on A375 cell.
文摘Flowing with the reform of the hot water heating method in China, heat meter will enter into households in the near future. A portable ultrasonic heat meter is designed in this paper. The meter uses chip microprocessor MSP430F437 as the data process core, and uses ultrasonic flow sensor to measure flow rate of the hot water, and capture input and output temperatures of the hot water using the thermal resistance sensor Ptl000, and then household energy consumption is calculated via temperature difference between input temperature and output temperature of the hot water multiplied by volume of hot water that is calculated though flow rate integration of hot water. In order to test the performance of the proposed heat meter, experiments is carried out. Both the temperature and flow measurement results satisfy the requirements of accuracy and the heat meter is effective in the heat measurement.
