Objective: To study the 4977-bp deletion of mitochondiral DNA in lung cancer, adjacent normal tissue and health lung and its significance in the development of cancer. Methods:Thirty-seven matched lung cancer/adjacent...Objective: To study the 4977-bp deletion of mitochondiral DNA in lung cancer, adjacent normal tissue and health lung and its significance in the development of cancer. Methods:Thirty-seven matched lung cancer/adjacent histologically normal and 20 “true” normal lung tissue samples from patients without lung cancer were analyzed by long PCR technique. Results: Mitochondrial DNA 4977-bp deletion was detected in 54.1% (20/37) of lung cancers, 59.5% (22/37) of adjacent normal and 30.0% (6/30) of “true” normal lung tissues. The correlation of 4977-bp deletion with age and smoking factors was present in our data. Conclusion: Mitochondrial DNA 4977-bp deletion is not specific to lung cancer and unlikely to play an important role in carcinogenesis, and may only reflect the environmental and genetic influences during tumor progression.展开更多
Alterations of mitochondria DNA(mtDNA)4977 bp common deletion(CD)and mtDNA copy number induced by ionizing radiation were observed in human different cell lines and total body irradiation patients.However,only few exp...Alterations of mitochondria DNA(mtDNA)4977 bp common deletion(CD)and mtDNA copy number induced by ionizing radiation were observed in human different cell lines and total body irradiation patients.However,only few experiments have evaluated the levels of the CD and mtDNA copy number in human peripheral blood exposed to ionizing radiation till now.The aim of this study is to analyze the mtDNA alterations in irradiated human peripheral blood from healthy donors as well as to explore their feasibility as biomarkers for constructing new biodosimeter.Peripheral blood samples were collected from six healthy donors,and exposed to 60Co gamma ray with the doses of 0 Gy,1 Gy,2 Gy,3 Gy,4 Gy and 5 Gy.Levels of the CD and mtDNA copy number in irradiated samples after 2h or 24h incubation were detected using TaqMan real-time PCR,and the CD ratio was calculated.The results showed that the mean of the CD ratio and the CD copy number exhibited a dose-dependent increase 2 h in the dose range from 0–5 Gy,and of the mtDNA copy number significantly increased 24 h in irradiated groups compared with 0 Gy group after irradiation.It indicates that the parameters in human peripheral blood may be considered as molecular biomarkers to applying construction of new biodosimeter.展开更多
Objective:To detect mitochondrial DNA(mtDNA) 4977 bp deletion in the oocytes of patients with diminished ovarian reserve,and help to understand its impact on the reproductive potential. Methods:Eighty infertile patien...Objective:To detect mitochondrial DNA(mtDNA) 4977 bp deletion in the oocytes of patients with diminished ovarian reserve,and help to understand its impact on the reproductive potential. Methods:Eighty infertile patients treated with in vitro fertilization-embryo transfer(IVF-ET) from Mar to Sep 2008 in Xiangya Hospital were recruited and divided into two groups according to ovarian reserve:diminished ovarian reserve(DOR,with basal follicular stimulating hormone(bFSH) levels≥12 U/L and/or bilateral antral follicle count(AFC)≤5) and normal ovarian reserve(NOR,with bFSH levels<8 U/L,luteinizing hormone(LH) levels<6 U/L,estradiol levels of 110-184 pmol/L,and unilateral AFC 3-10).110 unfertilized MII oocytes were collected on the day of embryo-transfer(46 in DOR group,64 in NOR group) and mtDNA4977 bp deletion was detected by polymerase chain reaction(PCR). Results:A significantly higher rate of mtDNA 4977 bp deletion was observed in the MII oocytes in DOR group (48.8%) than in NOR group(18.8%)(P<0.05).Logistic regression analysis showed that patients' age was not significantly correlated with the deletion rate. Conclusion:The high mtDNA4977 bp deletion rate in DOR patients' oocytes may be associated with their decreased oocyte developmental potential.展开更多
文摘Objective: To study the 4977-bp deletion of mitochondiral DNA in lung cancer, adjacent normal tissue and health lung and its significance in the development of cancer. Methods:Thirty-seven matched lung cancer/adjacent histologically normal and 20 “true” normal lung tissue samples from patients without lung cancer were analyzed by long PCR technique. Results: Mitochondrial DNA 4977-bp deletion was detected in 54.1% (20/37) of lung cancers, 59.5% (22/37) of adjacent normal and 30.0% (6/30) of “true” normal lung tissues. The correlation of 4977-bp deletion with age and smoking factors was present in our data. Conclusion: Mitochondrial DNA 4977-bp deletion is not specific to lung cancer and unlikely to play an important role in carcinogenesis, and may only reflect the environmental and genetic influences during tumor progression.
基金Supported by grants from the building project for the National Key Clinical Special Department of China(No.2011-17)the Medical Science and Technology Foundation of Henan Province(No.201204123)the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘Alterations of mitochondria DNA(mtDNA)4977 bp common deletion(CD)and mtDNA copy number induced by ionizing radiation were observed in human different cell lines and total body irradiation patients.However,only few experiments have evaluated the levels of the CD and mtDNA copy number in human peripheral blood exposed to ionizing radiation till now.The aim of this study is to analyze the mtDNA alterations in irradiated human peripheral blood from healthy donors as well as to explore their feasibility as biomarkers for constructing new biodosimeter.Peripheral blood samples were collected from six healthy donors,and exposed to 60Co gamma ray with the doses of 0 Gy,1 Gy,2 Gy,3 Gy,4 Gy and 5 Gy.Levels of the CD and mtDNA copy number in irradiated samples after 2h or 24h incubation were detected using TaqMan real-time PCR,and the CD ratio was calculated.The results showed that the mean of the CD ratio and the CD copy number exhibited a dose-dependent increase 2 h in the dose range from 0–5 Gy,and of the mtDNA copy number significantly increased 24 h in irradiated groups compared with 0 Gy group after irradiation.It indicates that the parameters in human peripheral blood may be considered as molecular biomarkers to applying construction of new biodosimeter.
文摘Objective:To detect mitochondrial DNA(mtDNA) 4977 bp deletion in the oocytes of patients with diminished ovarian reserve,and help to understand its impact on the reproductive potential. Methods:Eighty infertile patients treated with in vitro fertilization-embryo transfer(IVF-ET) from Mar to Sep 2008 in Xiangya Hospital were recruited and divided into two groups according to ovarian reserve:diminished ovarian reserve(DOR,with basal follicular stimulating hormone(bFSH) levels≥12 U/L and/or bilateral antral follicle count(AFC)≤5) and normal ovarian reserve(NOR,with bFSH levels<8 U/L,luteinizing hormone(LH) levels<6 U/L,estradiol levels of 110-184 pmol/L,and unilateral AFC 3-10).110 unfertilized MII oocytes were collected on the day of embryo-transfer(46 in DOR group,64 in NOR group) and mtDNA4977 bp deletion was detected by polymerase chain reaction(PCR). Results:A significantly higher rate of mtDNA 4977 bp deletion was observed in the MII oocytes in DOR group (48.8%) than in NOR group(18.8%)(P<0.05).Logistic regression analysis showed that patients' age was not significantly correlated with the deletion rate. Conclusion:The high mtDNA4977 bp deletion rate in DOR patients' oocytes may be associated with their decreased oocyte developmental potential.
