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稻瘟病菌(Magnaporthe grisea)诱导的水稻早期反应基因ER1的5’端cDNA片段克隆及序列分析 被引量:2
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作者 周建明 朱群 白永延 《植物生理学报(0257-4829)》 CSCD 1999年第4期413-416,共4页
The 5’ end cDNA fragment(ER1’) of early responsive gene(ER1) of rice(Oryza sativa L. subsp. japonica No.4) induced by blast fungus M. grisea has been isolated and sequenced (606 bp) by 5’RACE technique(Fig. 2). DNA... The 5’ end cDNA fragment(ER1’) of early responsive gene(ER1) of rice(Oryza sativa L. subsp. japonica No.4) induced by blast fungus M. grisea has been isolated and sequenced (606 bp) by 5’RACE technique(Fig. 2). DNA sequence analysis showed that ER1’ (606 bp) can encode 134 amino acids and there are 309 bp nucleotides located at the 3’ end of the untranslated region of mRNA of ER1 gene(Fig. 3). The 89 bp overlapping fragment was found between the 5’ end of ER1 cDNA fragment and the 3’ end of ER1’(Fig. 3). The partial identities of amino acid sequence deduced from ER1’ with an unknown protein(gene accession number AC002409) of Arabidopsis thaliana was 82%(Fig. 4). 展开更多
关键词 水稻 稻瘟病菌 ER1基因 5'cdna片段 5’RACE
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