[ Objective] The aim of the study is to clone the 5＇ flanking region of Sporamin A gene from sweet potato. [ Method ] The sweet potato ＂Xushu 18＂ was used to amplify the 5＇ flanking region of Sporamin A gene using...[ Objective] The aim of the study is to clone the 5＇ flanking region of Sporamin A gene from sweet potato. [ Method ] The sweet potato ＂Xushu 18＂ was used to amplify the 5＇ flanking region of Sporamin A gene using specifically designed primers and then target fragment was analyzed by PLACE and PlantCare online. [ Result ] Besides the conserved elements including TATA-box and CAAT-box, the cis-acting elements including sucrose responsive element CMSRE1, SP8 acting site and some other regulatory sequence such as MYB binding site were also found in Spo A promoter sequence. The results suggest that the promoter has sucrose responsive function. [ Conclusion ] The study provided reference to reveal the regulation law of Spo A, and to develop high-level expression vectors promoted by Spo A promoter.展开更多
The cDNA molecule encoding the mouse GABA transporter gene (GAT-1) was used as probe for selecting GAT-1 gene from mouse genomic library. A positive clone, harboring the whole open reading frame of the GAT-1 protein a...The cDNA molecule encoding the mouse GABA transporter gene (GAT-1) was used as probe for selecting GAT-1 gene from mouse genomic library. A positive clone, harboring the whole open reading frame of the GAT-1 protein and designated as MGABAT-G, was fished out from the library, the 5’ proximal region and nitron 1 were sequenced and analysed, and low homology was found in the above region between GAT-1 genes from mouse and human except some short conserved sequences. The DNA-protein interactions between DNA fragments containing the conserved sequences in the 5’ proximal region and nuclear proteins from different tissues of mouse were studied by means of gel-shift assay, and Southern-Western blot. The results indicate a possible positive-negative regulation mode controlling the expression of the mouse GAT-1 gene.展开更多
基金Project of the High and NewTechnology Industrial Development for Ordinary College in Jiangsu Province(JHB04043)Six High-Tech Talents Program of Jiangsu Province~~
文摘[ Objective] The aim of the study is to clone the 5＇ flanking region of Sporamin A gene from sweet potato. [ Method ] The sweet potato ＂Xushu 18＂ was used to amplify the 5＇ flanking region of Sporamin A gene using specifically designed primers and then target fragment was analyzed by PLACE and PlantCare online. [ Result ] Besides the conserved elements including TATA-box and CAAT-box, the cis-acting elements including sucrose responsive element CMSRE1, SP8 acting site and some other regulatory sequence such as MYB binding site were also found in Spo A promoter sequence. The results suggest that the promoter has sucrose responsive function. [ Conclusion ] The study provided reference to reveal the regulation law of Spo A, and to develop high-level expression vectors promoted by Spo A promoter.
文摘The cDNA molecule encoding the mouse GABA transporter gene (GAT-1) was used as probe for selecting GAT-1 gene from mouse genomic library. A positive clone, harboring the whole open reading frame of the GAT-1 protein and designated as MGABAT-G, was fished out from the library, the 5’ proximal region and nitron 1 were sequenced and analysed, and low homology was found in the above region between GAT-1 genes from mouse and human except some short conserved sequences. The DNA-protein interactions between DNA fragments containing the conserved sequences in the 5’ proximal region and nuclear proteins from different tissues of mouse were studied by means of gel-shift assay, and Southern-Western blot. The results indicate a possible positive-negative regulation mode controlling the expression of the mouse GAT-1 gene.
