In the course of C_5 fraction separation the diolefin compo-nents are apt to polymerize during extractive rectificationprocesses,and the polymers thus formed are prone to plugthe trays and reboiler,which is a beast se...In the course of C_5 fraction separation the diolefin compo-nents are apt to polymerize during extractive rectificationprocesses,and the polymers thus formed are prone to plugthe trays and reboiler,which is a beast seriously constrainingthe extended and smooth operation of process units.Thusthe Chemical Engineering Research Institute of展开更多
A newly isolated bacterium, named as AUH-JLC257, was found to be capable of bioconverting isoflavone genistein to 5-hydroxy-equol under anaerobic conditions. The metabolite 5-hydroxy-equol was identified by using UV s...A newly isolated bacterium, named as AUH-JLC257, was found to be capable of bioconverting isoflavone genistein to 5-hydroxy-equol under anaerobic conditions. The metabolite 5-hydroxy-equol was identified by using UV spectrum, electrospray ionization mass spectrometry （ESI-MS） as well as IH and 13C NMR analyses. Chiral stationary-phase high-performance liquid chromatography analysis and specific rotation examination demonstrated that the bio-synthesized 5-hydroxy-equol was just （-）-5-hydroxy-equol. The average bioconverting rate was 83.1%, and the strain AUH-JLC257 could efficiently transform genistein at a maximal substrate concentration of 0.6 mmol/L. We, for the first time, showed that the bio-synthesized 5-hydroxy-equol had 1,1-diphenyl-2-picrylhydrazyl （DPPH） radical-scavenging activity at concentrations as low as 3.3μmol/L. In addition, the 16S rRNA gene sequence （1401 bp） of the bacterium strain AUH-JLC257 showed the highest similarity （99.27%） to that of Slackia equolifaciens strain DZE.展开更多
文摘In the course of C_5 fraction separation the diolefin compo-nents are apt to polymerize during extractive rectificationprocesses,and the polymers thus formed are prone to plugthe trays and reboiler,which is a beast seriously constrainingthe extended and smooth operation of process units.Thusthe Chemical Engineering Research Institute of
基金National Natural Science Foundation of China(Grant No.31170058)the Service Center for Experts and Scholars of Hebei Province(Grant No.CPRC027)
文摘A newly isolated bacterium, named as AUH-JLC257, was found to be capable of bioconverting isoflavone genistein to 5-hydroxy-equol under anaerobic conditions. The metabolite 5-hydroxy-equol was identified by using UV spectrum, electrospray ionization mass spectrometry （ESI-MS） as well as IH and 13C NMR analyses. Chiral stationary-phase high-performance liquid chromatography analysis and specific rotation examination demonstrated that the bio-synthesized 5-hydroxy-equol was just （-）-5-hydroxy-equol. The average bioconverting rate was 83.1%, and the strain AUH-JLC257 could efficiently transform genistein at a maximal substrate concentration of 0.6 mmol/L. We, for the first time, showed that the bio-synthesized 5-hydroxy-equol had 1,1-diphenyl-2-picrylhydrazyl （DPPH） radical-scavenging activity at concentrations as low as 3.3μmol/L. In addition, the 16S rRNA gene sequence （1401 bp） of the bacterium strain AUH-JLC257 showed the highest similarity （99.27%） to that of Slackia equolifaciens strain DZE.
