[Objective]The study aimed to screen the starch-degrading bacterium in bagasse and carry on the identification of strains s2g5-1 and s3g4-8.[Method]By using a variety of selective media,varieties of starch degrading b...[Objective]The study aimed to screen the starch-degrading bacterium in bagasse and carry on the identification of strains s2g5-1 and s3g4-8.[Method]By using a variety of selective media,varieties of starch degrading bacterium were isolated from the sugar cane bagasse form different stages of natural fermentation,then,primary screening and secondary screening were performed.[Result] Starch-degrading strains s2g5-1 and s3g4-8 were screened,and they were identified as Bacillus amyloliquefaciens according to their morphological,physiological,biochemical and molecular characteristics.[Conclusion]The research provided theoretical basis for factory application of bagasse.展开更多
IEC-60870-5-104规约作为采用标准传输协议子集的IEC60870-5-101的网络访问,由于涉及到网络编程以及颁布时间较晚,在电力系统中还处于推广阶段。目前对104规约的探讨不论是应用还是研究都比较深入,并且取得了一定的成绩,但在软件设计方...IEC-60870-5-104规约作为采用标准传输协议子集的IEC60870-5-101的网络访问,由于涉及到网络编程以及颁布时间较晚,在电力系统中还处于推广阶段。目前对104规约的探讨不论是应用还是研究都比较深入,并且取得了一定的成绩,但在软件设计方面,还没有形成较统一的开发模式,迫切需要一个好的设计方法和工具帮助开发者设计开发通信软件。基于有限状态机理论提出一种设计方法用于104规约的实现。在这种设计方法中,结合通信协议的分层思想进行软件的总体设计,同时应用有限状态机(Finite State Machine,FSM)理论对104规约建模,抽象出有限状态机模型,并提出一种面向对象的FSM实现框架及其配置工具,利用配置工具自动实现FSM。展开更多
pHNC3, a suicide plasmid encoding luxAB and scaB genes in Tn5,can be transfered into Rhizobium through tri-parental mating and thenthe Rhizobium is labelled by luxAB. Since the levansucrasc activitycoded by sacB gene ...pHNC3, a suicide plasmid encoding luxAB and scaB genes in Tn5,can be transfered into Rhizobium through tri-parental mating and thenthe Rhizobium is labelled by luxAB. Since the levansucrasc activitycoded by sacB gene can cause the sucrose sensitivity on Gram-negativebacteria,the sacB gene allowed direct selection for the deletion or curingof plasmide, because only strains which no longer contain an activesacB gene are able to grow on media containing sucrose. This negativeselecting model can be used for direct labelling the mega-plasmid ofRhizobium,or even getting Rhizobium deriyatives partial deleted or curedtheir mega-plasmids.展开更多
基金Supported by the Central Public-interest Scientific Institution Basal Research Fund(2008hzs1J021,2009hzs1J033)~~
文摘[Objective]The study aimed to screen the starch-degrading bacterium in bagasse and carry on the identification of strains s2g5-1 and s3g4-8.[Method]By using a variety of selective media,varieties of starch degrading bacterium were isolated from the sugar cane bagasse form different stages of natural fermentation,then,primary screening and secondary screening were performed.[Result] Starch-degrading strains s2g5-1 and s3g4-8 were screened,and they were identified as Bacillus amyloliquefaciens according to their morphological,physiological,biochemical and molecular characteristics.[Conclusion]The research provided theoretical basis for factory application of bagasse.
文摘IEC-60870-5-104规约作为采用标准传输协议子集的IEC60870-5-101的网络访问,由于涉及到网络编程以及颁布时间较晚,在电力系统中还处于推广阶段。目前对104规约的探讨不论是应用还是研究都比较深入,并且取得了一定的成绩,但在软件设计方面,还没有形成较统一的开发模式,迫切需要一个好的设计方法和工具帮助开发者设计开发通信软件。基于有限状态机理论提出一种设计方法用于104规约的实现。在这种设计方法中,结合通信协议的分层思想进行软件的总体设计,同时应用有限状态机(Finite State Machine,FSM)理论对104规约建模,抽象出有限状态机模型,并提出一种面向对象的FSM实现框架及其配置工具,利用配置工具自动实现FSM。
文摘pHNC3, a suicide plasmid encoding luxAB and scaB genes in Tn5,can be transfered into Rhizobium through tri-parental mating and thenthe Rhizobium is labelled by luxAB. Since the levansucrasc activitycoded by sacB gene can cause the sucrose sensitivity on Gram-negativebacteria,the sacB gene allowed direct selection for the deletion or curingof plasmide, because only strains which no longer contain an activesacB gene are able to grow on media containing sucrose. This negativeselecting model can be used for direct labelling the mega-plasmid ofRhizobium,or even getting Rhizobium deriyatives partial deleted or curedtheir mega-plasmids.
基金supported by a key project of Natural Science Foundation of China (No.30430580)the State Forestry Administration,People’s Republic of China (No.20070430)
