Background：Psychocardiological researches have suggested a central role of 5-hydroxytryptamine （5-HT） on psychocardiological mechanism.This study aimed to further explore the central role of 5-HT and pretreatment e...Background：Psychocardiological researches have suggested a central role of 5-hydroxytryptamine （5-HT） on psychocardiological mechanism.This study aimed to further explore the central role of 5-HT and pretreatment effects of XinLingWan on rats with myocardial infarction （M I） and/or depression.Methods：Ninety Sprague-Dawley rats were randomly divided into three groups：MI group,depression group,and MI ＋ depression group （n 30 in each group）.Each group was then divided into three subgroups （n =10 in each subgroup）：a negative control subgroup （NCS）,a Western medicine subgroup （WMS）,and a traditional Chinese medicine subgroup （TCMS）,which were received pretreatment once a day for 4 weeks by saline,20 mg/kg sertraline mixed with 2 ml saline,and 40 mg/kg XingLingWan mixed with 2 ml saline,respectively.Different rat models were established after different pretreatments.Rats were then sacrificed for detection of serum 5-HT,platelet 5-HT,5-HT2.A receptors （5-HT2AR）,and serotonin transporter （SERT）.Data were analyzed by one-way analysis of variance （ANOVA） and least-significant difference （LSD） testing.Results：M I group：compared with NCS,there was a significant increase in WMS and TCMS of serum 5-HT （176.15 ± 11.32 pg/ml vs.334.50 ± 29.09 pg/ml and 474.04 ± 10.86 pg/ml,respectively,both P =0.000）,platelet 5-HT （129.74 ± 27.17 pg/ml vs.322.24 ± 11.60 pg/ml and 340.4 5 ± 17.99 pg/ml,respectively,both P =0.000）;depression group：compared with NCS,there was a significant increase in WMS and TCMS of serum 5-HT （194.69 ± 5.09 pg/ml vs.326.21 ± 39.98 pg/ml and 456.33 ± 23.12 pg/ml,respectively,both P =0.000）,platelet 5-HT （175.15 ± 4.07 pg/ml vs.204.56 ± 18.59 pg/ml and 252.03 ± 22.26 pg/ml,respectively,P =0.004 and P 0.000,respectively）;MI ＋ depression group：compared with NCS,there was a significant increase in both WMS and TCMS of serum 5-HT （182.50 ± 10.23 pg/ml vs.372.55 ± 52.23 pg/ml and 441.76 ± 23.38 pg/ml,respectively,both P =0.000） and platelet 5-HT （180.83 ± 11.08 pg/ml vs.221.12 ± 22.23 pg/ml and 265.37 ± 29.49 pg/ml,respectively,P =0.011 and P =0.000,respectively）.Conclusions：By elevating the amount of 5-HT and modulating 5-HT2AR and SERT levels in serum and platelets,XinLingWan and sertraline were found to exert pretreatment effect on rat models of MI and/or depression.展开更多
Background： To evaluate whether serotonin （5-HT）, 5-HT2A receptor （5-HT2AR）, and 5-HT transporter （serotonin transporter [SERT]） are associated with different disease states of depression, myocardial infarction...Background： To evaluate whether serotonin （5-HT）, 5-HT2A receptor （5-HT2AR）, and 5-HT transporter （serotonin transporter [SERT]） are associated with different disease states of depression, myocardial infarction （MI） and MI co-exist with depression in Sprague-Dawley rats. Methods： After established the animal model of four groups include control, depression, MI and MI with depression, we measured 5-HT, 5-HT2AR and SERT from serum and platelet lysate.Results： The serum concentration of 5-HT in depression rats decreased significantly compared with the control group （303.25 ± 9.99 vs. 352.98 ±13.73; P =0.000）, while that in MI group increased （381.78 ±14.17 vs. 352.98 ±13.73; P = 0.000）. However, the depression ＋ MI group had no change compared with control group （360.62 ±11.40 vs. 352.98 ±13.73; P = 0.036）. The changes of the platelet concentration of 5-HT in the depression, MI, and depression ＋ MI group were different from that of serum. The levels of 5-HT in above three groups were lower than that in the control group （380.40 ± 17.90, 387.75 ±22.28,246.40 ±18.99 vs. 500.29 ±20.91 ; P = 0.000）. The platelet lysate concentration of 5-HT2AR increased in depression group, MI group, and depression ＋ MI group compared with the control group （370.75 ±14.75,393.47 ±15.73,446.66 ±18.86 vs. 273.66 ±16.90; P= 0.000）. The serum and platelet concentration of SERT in the depression group, MI group and depression ＋ MI group were all increased compared with the control group （527.51 ±28.32, 602.02 ±23.32, 734.76 ±29.59 vs. 490.56 ±16.90; P 0,047, P = 0.000, P = 0.000 in each and 906.38 ±51.84, 897.33 ±60.34, 1030.17 ±58.73 vs. 708.62 ±51.15; P = 0.000 in each）. Conclusions： The concentration of 5-HT2AR in platelet lysate and SERT in serum and platelet may be involved in the pathway of MI with depression. Further studies should examine whether elevated 5-HT2AR and SERT may contribute to the biomarker in MI patients with depression.展开更多
Background： Our previous studies have demonstrated that the levels of 5-hydroxytryptamine （5-HT） and 5-HT 2A receptor （5-HT2AR） in serum and platelet were associated with depression and myocardial infarction （MI...Background： Our previous studies have demonstrated that the levels of 5-hydroxytryptamine （5-HT） and 5-HT 2A receptor （5-HT2AR） in serum and platelet were associated with depression and myocardial infarction （MI）, and pretreatment with ginseng fruit saponins （GFS） before MI and depression had an effect on the 5-HT system. In this study, the effects of GFS on the 5-HT system in the Sprague-Dawley （SD） rats with MI, depression, and MI ＋ depression were evaluated. Methods： A total of eighty SD rats were allocated to four groups： MI, depression, MI ＋ depression, and control groups （n = 20 in each group）. Each group included two subgroups （n = 10 in each subgroup）： Saline treatment subgroup and GFS treatment subgroup. The levels of 5-HT, 5-HT2AR, and serotonin transporter （SERT） were quantified in serum, platelet lysate, and brain tissue through the enzyme-linked immunosorbent assay method, respectively. Results： Compared with those in the saline treatment subgroups, the levels of 5-HT in serum and platelet lysate statistically significantly increased in the GFS treatment subgroups of MI, depression, and MI ＋ depression groups （serum： all P = 0.000; platelet lysate： P = 0.002, 0.000, 0.000, respectively）. However, the 5-HT levels in brain homogenate significantly decreased in the GFS treatment subgroups compared with those in the saline treatment subgroups in MI and depression groups （P = 0.025 and 0.044 respectively）, and no significant difference was observed between saline and GFS treatment subgroups in MI ＋ depression group （P = 0.663）. Compared with that in GFS treatment subgroup of control group, the 5-HT2AR levels in the platelet lysate significantly decreased in GFS treatment subgroups of MI, depression, and MI ＋ depression groups （all P = 0.000）. Compared to those in the saline treatment subgroups, the serum SERT levels significantly decreased in the GFS treatment subgroups in MI, depression, and MI ＋ depression groups （P = 0.009, 0.038, and P = 0.001, respectively）, while the SERT levels of platelet lysate significantly decreased in GFS treatment subgroup of MI group （P = 0.000）, significantly increased in GFS treatment subgroup of depression group （P = 0.019）, and slightly changed in GFS treatment subgroup of MI ＋ depression group （P = 0.219）. No significant changes for SERT levels in brain homogenate could be found between the saline and GFS treatment subgroups in MI, depression, and MI ＋ depression groups （P = 0.421, 0.076 and P = 0.642）. Conclusions： This study indicated that GFS might inhibit the reuptake of 5-HT from serum to platelet according to decreased 5-HT2AR in platelet and SERT in serum and platelet. The change of 5-HT in serum after GFS treatment was inconsistent with that in the brain. It seemed that GFS could not pass through the blood-brain barrier to affect the central serotonergic system.展开更多
文摘Background：Psychocardiological researches have suggested a central role of 5-hydroxytryptamine （5-HT） on psychocardiological mechanism.This study aimed to further explore the central role of 5-HT and pretreatment effects of XinLingWan on rats with myocardial infarction （M I） and/or depression.Methods：Ninety Sprague-Dawley rats were randomly divided into three groups：MI group,depression group,and MI ＋ depression group （n 30 in each group）.Each group was then divided into three subgroups （n =10 in each subgroup）：a negative control subgroup （NCS）,a Western medicine subgroup （WMS）,and a traditional Chinese medicine subgroup （TCMS）,which were received pretreatment once a day for 4 weeks by saline,20 mg/kg sertraline mixed with 2 ml saline,and 40 mg/kg XingLingWan mixed with 2 ml saline,respectively.Different rat models were established after different pretreatments.Rats were then sacrificed for detection of serum 5-HT,platelet 5-HT,5-HT2.A receptors （5-HT2AR）,and serotonin transporter （SERT）.Data were analyzed by one-way analysis of variance （ANOVA） and least-significant difference （LSD） testing.Results：M I group：compared with NCS,there was a significant increase in WMS and TCMS of serum 5-HT （176.15 ± 11.32 pg/ml vs.334.50 ± 29.09 pg/ml and 474.04 ± 10.86 pg/ml,respectively,both P =0.000）,platelet 5-HT （129.74 ± 27.17 pg/ml vs.322.24 ± 11.60 pg/ml and 340.4 5 ± 17.99 pg/ml,respectively,both P =0.000）;depression group：compared with NCS,there was a significant increase in WMS and TCMS of serum 5-HT （194.69 ± 5.09 pg/ml vs.326.21 ± 39.98 pg/ml and 456.33 ± 23.12 pg/ml,respectively,both P =0.000）,platelet 5-HT （175.15 ± 4.07 pg/ml vs.204.56 ± 18.59 pg/ml and 252.03 ± 22.26 pg/ml,respectively,P =0.004 and P 0.000,respectively）;MI ＋ depression group：compared with NCS,there was a significant increase in both WMS and TCMS of serum 5-HT （182.50 ± 10.23 pg/ml vs.372.55 ± 52.23 pg/ml and 441.76 ± 23.38 pg/ml,respectively,both P =0.000） and platelet 5-HT （180.83 ± 11.08 pg/ml vs.221.12 ± 22.23 pg/ml and 265.37 ± 29.49 pg/ml,respectively,P =0.011 and P =0.000,respectively）.Conclusions：By elevating the amount of 5-HT and modulating 5-HT2AR and SERT levels in serum and platelets,XinLingWan and sertraline were found to exert pretreatment effect on rat models of MI and/or depression.
文摘Background： To evaluate whether serotonin （5-HT）, 5-HT2A receptor （5-HT2AR）, and 5-HT transporter （serotonin transporter [SERT]） are associated with different disease states of depression, myocardial infarction （MI） and MI co-exist with depression in Sprague-Dawley rats. Methods： After established the animal model of four groups include control, depression, MI and MI with depression, we measured 5-HT, 5-HT2AR and SERT from serum and platelet lysate.Results： The serum concentration of 5-HT in depression rats decreased significantly compared with the control group （303.25 ± 9.99 vs. 352.98 ±13.73; P =0.000）, while that in MI group increased （381.78 ±14.17 vs. 352.98 ±13.73; P = 0.000）. However, the depression ＋ MI group had no change compared with control group （360.62 ±11.40 vs. 352.98 ±13.73; P = 0.036）. The changes of the platelet concentration of 5-HT in the depression, MI, and depression ＋ MI group were different from that of serum. The levels of 5-HT in above three groups were lower than that in the control group （380.40 ± 17.90, 387.75 ±22.28,246.40 ±18.99 vs. 500.29 ±20.91 ; P = 0.000）. The platelet lysate concentration of 5-HT2AR increased in depression group, MI group, and depression ＋ MI group compared with the control group （370.75 ±14.75,393.47 ±15.73,446.66 ±18.86 vs. 273.66 ±16.90; P= 0.000）. The serum and platelet concentration of SERT in the depression group, MI group and depression ＋ MI group were all increased compared with the control group （527.51 ±28.32, 602.02 ±23.32, 734.76 ±29.59 vs. 490.56 ±16.90; P 0,047, P = 0.000, P = 0.000 in each and 906.38 ±51.84, 897.33 ±60.34, 1030.17 ±58.73 vs. 708.62 ±51.15; P = 0.000 in each）. Conclusions： The concentration of 5-HT2AR in platelet lysate and SERT in serum and platelet may be involved in the pathway of MI with depression. Further studies should examine whether elevated 5-HT2AR and SERT may contribute to the biomarker in MI patients with depression.
文摘Background： Our previous studies have demonstrated that the levels of 5-hydroxytryptamine （5-HT） and 5-HT 2A receptor （5-HT2AR） in serum and platelet were associated with depression and myocardial infarction （MI）, and pretreatment with ginseng fruit saponins （GFS） before MI and depression had an effect on the 5-HT system. In this study, the effects of GFS on the 5-HT system in the Sprague-Dawley （SD） rats with MI, depression, and MI ＋ depression were evaluated. Methods： A total of eighty SD rats were allocated to four groups： MI, depression, MI ＋ depression, and control groups （n = 20 in each group）. Each group included two subgroups （n = 10 in each subgroup）： Saline treatment subgroup and GFS treatment subgroup. The levels of 5-HT, 5-HT2AR, and serotonin transporter （SERT） were quantified in serum, platelet lysate, and brain tissue through the enzyme-linked immunosorbent assay method, respectively. Results： Compared with those in the saline treatment subgroups, the levels of 5-HT in serum and platelet lysate statistically significantly increased in the GFS treatment subgroups of MI, depression, and MI ＋ depression groups （serum： all P = 0.000; platelet lysate： P = 0.002, 0.000, 0.000, respectively）. However, the 5-HT levels in brain homogenate significantly decreased in the GFS treatment subgroups compared with those in the saline treatment subgroups in MI and depression groups （P = 0.025 and 0.044 respectively）, and no significant difference was observed between saline and GFS treatment subgroups in MI ＋ depression group （P = 0.663）. Compared with that in GFS treatment subgroup of control group, the 5-HT2AR levels in the platelet lysate significantly decreased in GFS treatment subgroups of MI, depression, and MI ＋ depression groups （all P = 0.000）. Compared to those in the saline treatment subgroups, the serum SERT levels significantly decreased in the GFS treatment subgroups in MI, depression, and MI ＋ depression groups （P = 0.009, 0.038, and P = 0.001, respectively）, while the SERT levels of platelet lysate significantly decreased in GFS treatment subgroup of MI group （P = 0.000）, significantly increased in GFS treatment subgroup of depression group （P = 0.019）, and slightly changed in GFS treatment subgroup of MI ＋ depression group （P = 0.219）. No significant changes for SERT levels in brain homogenate could be found between the saline and GFS treatment subgroups in MI, depression, and MI ＋ depression groups （P = 0.421, 0.076 and P = 0.642）. Conclusions： This study indicated that GFS might inhibit the reuptake of 5-HT from serum to platelet according to decreased 5-HT2AR in platelet and SERT in serum and platelet. The change of 5-HT in serum after GFS treatment was inconsistent with that in the brain. It seemed that GFS could not pass through the blood-brain barrier to affect the central serotonergic system.
