Photodynamic Therapy of Cancer Cells with Methanesulfonate Salts of 5-Aminolevulinic Acid and Its Derivatives

A series of 5-aminolevulinic acid and its alkylester methanesulfonates was exploited to photodynamic therapy（PDT） of human lymphocytic cells, U-937 in vitro. The PDT efficiency is influenced by the concentration and incubation time. Generally, for ALA and its alkylester methanesulfonates, the cell survival rate decreases and the accumulation ability of PplX increases with the concentration and incubation time. We found that the longer carbon chain methanesulfonates（C5-S, C6-S, C8-S） exhibit better PDT effect than ALA methanesulfonate. This possibly provides a promising route to the clinical application ofPplX-mediated PDT to cancer cell.

OPTIMIZATIONS FOR 5-AMINOLEVULINIC ACID BASED PHOTODYNAMIC THERAPY IN PURGING LEUKEMIA CELL HL60

Objective To optimize experimental parameters for the photosensitization of 5-aminolevulinic acid (ALA) in promyelocytic leukemia cell HL60 and compare them with normal human peripheral blood mononuclear cell (PBMC). Methods ALA incubation time, wavelength applied to irradiate, concentration of ALA incubated, irradiation fluence may modulate the effect of 5-aminolevulinic acid based Photodynamic Therapy (ALA-PDT).The high-pressure mercury lamps of 400W served as light source, the interference filter of 410nm, 432nm, 545nm, 577nm were used to select the specific wavelength. Fluorescence microscope was used to detect the fluorescence intensity and location of protoporphyrin IX (PpIX) endogenously produced by ALA. MTT assay was used to measure the survival of cell. Flow cytometry with ANNEXIN V FITC kit (contains annexin V FITC, binding buffer and PI) was used to detect the mode of cell death. Results ① 1mmol/L ALA incubated 1×105/mL HL60 cell line for 4 hours, the maximum fluorescence of ALA induced PpIX was detected in cytomembrane. ② Irradiated with 410nm for 14.4J/cm2 can result in the minimum survivability of HL60 cell. ③ The main mode of HL60 cell death caused by ALA-PDT is necrosis. Conclusion ALA for 1mmol/L, 4 hours for dark incubation time, 410nm for irradiation wavelength, 14.4J/cm2 for irradiation fluence were the optimal parameters to selectively eliminate promyelocytic leukemia cell HL60 by ALA based PDT. The photosensitization of ALA based PDT caused the necrosis of HL60 cell, so it could be used for inactivation of certain leukemia cells.

Recent advances in photodynamic diagnosis of gastriccancer using 5-aminolevulinic acid

Photodynamic diagnosis based on 5-aminolevulinic acidinduced protoporphyrin IX has been clinically applied in many fields based upon its evidenced efficacy and adequate safety. In order to establish a personalized medicine approach for treating gastric cancer patients, rapid intraoperative detection of malignant lesions has become important. Feasibility of photodynamic diagnosis using 5-aminolevulinic acid for gastric cancer patients has been investigated, especially for the detection of peritoneal dissemination and lymph node metastasis. This method enables intraoperative realtime fluorescence detection of peritoneal dissemination, exhibiting higher sensitivity than white light observation without histopathological examination. The method also enables detection of metastatic foci within excised lymph nodes, exhibiting a diagnostic accuracy comparable to that of a current molecular diagnostics technique. Although several complicating issues still need to be resolved, such as the effect of tissue autofluorescence and the insufficient depth penetration of excitation light, this simple and rapid method has the potential to become a useful diagnostic tool for gastric cancer, as well as urinary bladder cancer and glioma.

Development of a Photodynamic Diagnosis Method for Oral Squamous Cell Carcinoma Using 5-Aminolevulinic Acid and a Luminescence Plate Reader

Purpose: To establish a simple and accurate photodynamic diagnosis (PDD) method for oral squamous cell carcinoma (OSCC). Methods: OSCC cell lines HSC-2, HSC-3, HSC-4, and Sa3, and normal human oral keratinocytes (HOK) were used. First, we examined the amount of cells needed to detect differences in fluorescence intensities for PDD. OSCC cell lines were adjusted to concentrations of 1 × 104 (104), 1 × 105 (105), and 1 × 106 (106) cells/ml. The experimental groups comprised a group with 5-aminolevulinic acid (5-ALA (+)), and a group without 5-ALA (5-ALA (-)). For each OSCC cell line, 100 μl of each concentration of cells of the 5-ALA groups was seeded onto fluorescence plates, and fluorescence intensity was measured at 60-min intervals for 240 min. Results are expressed as the ratio of fluorescence intensity in 5-ALA (+) to 5-ALA (-). As cells at the concentration of 106 cells/ml provided the clearest results, fluorescence intensities of all cell lines were measured using this concentration at 20-min intervals for 700 min using the same methods. Results: The 5-ALA (+) to (-) ratio increased in a cell concentration-dependent manner at 240 min;the ratio was highest with 106 cells/ml and lowest with 104 cells/ml. With 106 cells/ml in the 5-ALA (+) group, fluorescence intensity increased in a metabolic time-dependent manner;the increase was highest in HSC-2 cells, followed by HSC-4 cells, HSC-3 cells, Sa3 cells, and HOK. Fluorescence intensity was significantly enhanced after 40 min in HSC-2, HSC-3, and HSC-4 cells, after 60 min in Sa3 cells, and after 100 min in HOK compared to the 5-ALA (-) group (P < 0.05). Moreover, fluorescence intensity was significantly increased in OSCC cell lines compared to HOK after 40 min. Conclusion: Early detection of OSCC is possible by screening only microplate reader measurements of fluorescence intensity for PDD.

Comparison of photodynamic therapy for skin cancers and pre-cancers with δ-aminolevulinic acid

Objective:The aim of the study was to compare the effects of photodynamic therapy(PDT) with δ-aminolevulinic acid(ALA) for patients with different kinds of skin cancers and pre-cancers.Methods:The present study enrolled seventyfive cases,which included 17 cases of actinic keratosis(AK),9 cases of Bowen's disease,11 cases of superficial basal cell carcinomas(BCC),23 cases of nodule basal cell carcinomas and 15 cases of squamous cell carcinomas(SCC),and every patient had single lesion.All patients were treated with 20% ALA topically and He-Ne laser weekly for three times,and followed up 1-3 years.Results:After therapy,the rates of complete reaction(CR) were 100% in AK lesions,77.8% in Bowen's diseases,90.9% in superficial BCCs,47.8% in nodule BCCs,and 50.3% in SCCs,which had significant differences among these five kinds of lesions(H = 18.27,P < 0.05).The therapeutic effectiveness of ALA-PDT for AK was superior to that of Bowen's disease(Q = 4.364,P < 0.05),superficial BCC(Q = 5.55,P < 0.01),SCC(Q = 8.94,P < 0.01) and nodule BCC(Q = 17.91,P < 0.01);the effect of Bowen's disease was better than that of SCC(Q = 7.8,P < 0.01),nodule BCC(Q = 13.44,P < 0.01);the effect of superficial BCC was better than that of SCC(Q = 9.73,P < 0.01),nodule BCC(Q = 16.28,P < 0.01),but similar with Bowen's disease(Q = 0.96,P > 0.05);the effect of SCC was better than that of nodule BCC(Q = 17.74,P < 0.01).Conclusion:Our study shows that therapeutic effectiveness of ALA-PDT for AK is best in five diseases,and Bowen's disease and superficial BCC are secondary,while nodule BCC and SCC are at the bottom.

5-aminolaevulinic Acid-photodynamic Therapy for the Treatment of Cervical Condylomata Acuminata

Objective To investigate the efficacy and safety of photodynamic therapy （PDT） with topical 5-aminolaevulinic acid （ALA） on cervical condylomata acuminata. Methods Patients with cervical condylomata （n=30） were allocated into primary and recurrent group, and were given topical ALA under occlusive dressing for 3 hours followed by irradiation with semiconductor laser at a dose of 100 Jcm 2 and a power of 100 roW. The treatment was repeated 7 days later if the lesion was not completely removed after the first treatment. Complete response rate and recurrence rate of wart lesions as well as rate of adverse reaction were analyzed. Results The total complete response rate of PDT was 100% and the total recurrence rate was 5% after 3 months of follow-up. Recurrence rate of recurrent group was significantly lower than that of prior managements （100%, P〈0.01）. The side effects of PDT in patients mainly included mild burning and/or stinging restricted to the illuminated areas, and was significant lower than their own control （25% vs. 100%, P〈0.05）. Conclusion Compared with conventional therapies, topical application of ALA-PDT is a simple, effective, safe, well-tolerated, and low recurrence rate treatment for cervical condylomata acuminata.

Clinical applications of 5-aminolevulinic acid-mediated fluorescence for gastric cancer

5-aminolevulinic acid(ALA) is a naturally occurring amino acid that is a protoporphyrin IX(Pp IX) precursor and a next-generation photosensitive substance. After exogenous administration of ALA, Pp IX specifically accumulates in cancer cells owing to the impaired metabolism of ALA to Pp IX in mitochondria, which results in a red fluorescence following irradiation with blue light and the formation of singlet oxygen. Fluorescence navigation by photodynamic diagnosis(PDD) using ALA provides good visualization and detection of gastric cancer lesions and is a potentially valuable diagnostic tool for gastric cancer for evaluating both the surgical resection margins and extension of the lesion. Furthermore, PDD using ALA might be used to detect peritoneal metastases during preoperative staging laparoscopy, where it could provide useful information for the selection of a therapeutic approach. Another promising application for this modality is in the evaluation of lymph node metastases. Photodynamic therapy(PDT) using ALA to cause selective damage based on the accumulation of a photosensitizer in malignant tissue is expected to be a non-invasive endoscopic treatment for superficial early gastric cancer. ALA has the potential to be used not only as a diagnostic agent but also as a therapeutic drug, resulting in a new strategy for cancer diagnosis and therapy. Here, we review the current use of PDD and PDT in gastric cancer and evaluate its future potential beyond conventional modalities combined with a light energy upconverter, a light-emitting diode and nearinfrared rays as light sources.

5-Aminolevulinic Acid (5-ALA): Analysis of Preclinical and Safety Literature

Problem: 5-ALA has been used for many years at relatively high dose amounts in single doses for photodynamic therapy and immunofluorescence of tumors. An analysis of compiled data relating to safety and any side-effects about the use of 5-ALA at low doses has not yet been published. Purpose: This report analyzes data about the safety of the use of 5-Aminolevulinic Acid (5-ALA) in low doses as a supplement over an extended period of time. Methods: This investigation is a systematic analysis of the current literature ((Medline, and SBI) and snowballing techniques) related to the safety and efficacy of 5-ALA in animals and humans. Clinical trials in progress using 5-ALA were also analyzed. Constant comparative analyses were used to synthesize the findings. Results: The safety of low-dose 5-ALA as a supplement has been demonstrated by animal and human studies. The results suggest that none of the investigations document the presence of symptoms or abnormal laboratory results of clinical significance. The minor laboratory changes documented were judged not clinically significant.

Low Frequency Ultrasonication Induced Antitumor Effect in 5-Aminolevulinic Acid Treated Malignant Glioma

We investigated the feasibility of sonodynamic therapy for glioma by low frequency ultrasoundwith5-aminolevulinic acid (5-ALA), a precursor of protoporphyrin IX (PpIX) in heme synthetic process. In vivo tumor model was made by inoculating human glioma cell line U87-MG subcutaneously in nude mice. The tumor was sonicated by 25-kHz ultrasound 4 hours following administration of 5-ALA. The tumor size decreased in 5-ALA administered (ALA(+)US(+)) mice, while increased in non-5-ALA administrated (ALA(-)US(+)) mice and non-sonicated mice (ALA(+)US(-)). The immunohistochemical analysis revealed an apoptotic change in tumor tissue of ALA(+)US(+) mice. The results showed the therapeutic effect of 25 kHz ultrasound for the glioma in 5-ALA administered tumor-bearing mice by inducing apoptotic change of tumor cells. This is a first report to elucidate the feasibility of therapeutic use of 25 kHz, relatively low frequency, ultrasound in sonodynamic therapy using 5-ALA as a sonosensitizer precursor. The utilization of this frequency will contribute to the development of sonodynamic therapy for gliomas and the spread of this technique in many hospitals that possess ultrasonic aspirators.

5-氨基酮戊酸光动力治疗对鲍温病皮损中p53 Caveolin-1表达的影响

目的:探讨5-氨基酮戊酸光动力疗法对鲍温病皮损中p53、Caveolin-1表达的影响及意义。方法:运用5-氨基酮戊酸光动力疗法治疗鲍温病及周围正常皮肤组织各40例,采用免疫组织化学技术(SP法)检测光动力治疗前后鲍温病及周围正常皮肤组织中p53、Caveolin-1的阳性细胞表达率。结果:治疗前p53蛋白在正常皮肤组织及BD中表达的阳性率分别为10%、40%(χ^(2)=11.202,P<0.001),差异有统计学意义。治疗后p53在正常皮肤组织及BD的阳性率为10%、5%(χ^(2)=0.712,P=0.399),两者间差异无统计学意义。治疗前后p53在BD中的阳性表达率差异有统计学意义(χ^(2)=14.811,P<0.001)。治疗前Caveolin-1在正常皮肤组织及BD中阳性表达率为15%、55%(χ^(2)=14.449,P<0.001),两者间差异有统计学意义。治疗后正常皮肤组织及BD中Caveolin-1的阳性率为5%、12.5%(χ^(2)=0.816,P=0.366),差异无统计学意义。治疗前后Caveolin-1在BD中的阳性表达率差异有统计学意义(χ^(2)=19.013,P<0.001)。BD中p53与Caveolin-1阳性表达呈正相关性(r=0.533,P=0.015)。结论:p53及Caveolin-1的高表达可能与BD的发生密切关联,且ALA-PDT能够抑制p53及Caveolin-1的表达,从而抑制疾病的发展。通过更大样本量的研究,p53及Caveolin-1可能会成为皮肤相关疾病的诊断工具,并为其治疗提供新的靶点。

CO_(2)LASER PLUS PHOTODYNAMIC THERAPY VERSUS CO_(2)LASER IN THE TREATMENT OF CONDYLOMA ACUMINATUM:A RANDOMIZED COMPARATIVE STUDY

To compare the efficacy and safety of CO_(2)laser plus 5-aminolevulinic acid(ALA)photodynamic therapy(PDT)with CO_(2)laser for the treatment of multiple condyloma acuminatum(CA),120 patients with multiple CA were allocated into two groups—combined group(CO_(2)laser plus ALA-PDT,n=60)and CO_(2)laser group(CO_(2)laser plus placebo-PDT,n=60).After CO_(2)laser,a 20%ALA or a placebo solution was applied to the CA area 3 h before illumination with red light(635 nm,100mW/cm^(2),80 J/cm^(2)).The treatment was repeated seven days after the first treatment if the lesions were not completely resolved.The complete response rate,recurrence rate and adverse effects in the two groups were analyzed.After two treatments,the complete response rates in the CO_(2)laser group and combined group were 100%(509/509)and 100%(507/507)in the CA(p>0:05),respectively.The recurrence rates in the CO_(2)laser group and combined group were 44.9%(229/509)and 10.6%(54/507)in the CA(p<0:05),respectively.The adverse effects in CO_(2)laser group was more than that in combined group.The combined group is a more effective treatment for multiple CA compared with CO_(2)laser group.T/S.Style the highlighted text as abstract.

5-氨基酮戊酸光动力疗法及CO_(2)激光分别联合复方利多卡因凝胶治疗尖锐湿疣的疗效及复发的相关性分析

目的探讨5-氨基酮戊酸光动力疗法(ALA-PDT)及二氧化碳(CO_(2))激光分别联合复方利多卡因凝胶治疗尖锐湿疣(CA)的疗效及复发的相关性分析。方法回顾性分析2021年1月至2023年1月徐州医科大学附属医院收治的121例CA患者的临床资料,根据治疗方式分为A组(n=62)、B组(n=59),A组采用ALA-PDT联合复方利多卡因凝胶治疗,B组采用CO_(2)激光联合复方利多卡因凝胶治疗,比较两组的临床疗效。治疗后6个月随访统计复发情况,并将患者分为复发组(n=34)和未复发组(n=87),分析CA患者复发的影响因素。结果A组临床总有效率高于B组,差异具有统计学意义(P<0.05)。多因素Logistic回归分析结果显示,多发CA、偶尔/经常使用或不使用安全套、2名及以上性伴侣、经常熬夜、CO_(2)激光联合复方利多卡因凝胶治疗是CA患者复发的独立危险因素(P<0.05)。结论ALA-PDT联合复方利多卡因凝胶治疗CA的疗效优于CO_(2)激光联合复方利多卡因凝胶治疗,CA数目、安全套使用情况、性伴侣数量、经常熬夜及治疗方式等是CA患者复发的相关因素,临床需重视以上因素并制定针对性干预措施以降低CA复发率。

行为改变轮理论在5-氨基酮戊酸光动力疗法治疗尖锐湿疣患者中的应用

目的探讨行为改变轮(BCW)理论在5-氨基酮戊酸光动力疗法(ALA-PDT)治疗尖锐湿疣(CA)患者中的应用。方法回顾性分析2021年10月至2023年5月89例在安徽省宣城市中心医院确诊CA的患者的临床资料,根据治疗方案的不同将患者分为观察组(n=47)和对照组(n=42)。两组均接受ALA-PDT治疗,对照组采用常规CA护理方式,观察组在对照组基础上采用BCW理论干预,均干预1个月。在干预前后,比较两组焦虑自评量表(SAS)评分、皮肤病生活指数(DLQI)、健康行为量表(HPL)和自我健康管理能力量表(ESCA)评分。随访3个月,比较两组人乳头瘤病毒(HPV)感染根除率。结果干预后,两组SAS评分均低于干预前,且观察组低于对照组(P<0.05);干预后,两组DLQI各维度评分均低于干预前,且观察组低于对照组(P<0.05);干预后,两组HPL、ESCA评分均高于干预前,且观察组高于对照组(P<0.05)。随访3个月后,观察组HPV感染根除率为89.36%(42/47),对照组HPV感染根除率为83.33%(35/42),两组无显著差异(P>0.05)。结论采用BCW理论干预ALA-PDT治疗的CA患者,可改善患者生活质量,提高其自我管理能力和促进健康行为。

5-氨基酮戊酸光动力学疗法联合干扰素栓对持续高危型人乳头瘤病毒感染患者病毒清除率及阴道微环境的影响

目的探究5-氨基酮戊酸光动力学疗法(ALA-PDT)联合干扰素栓对持续高危型人乳头瘤病毒(HPV)感染患者HPV清除率及阴道微环境的影响。方法选取2020年1月至2023年1月景德镇市第一人民医院收治的80例持续高危型HPV感染患者作为研究对象,采用随机数字表法将其分为对照组和观察组各40例,对照组采用干扰素栓治疗,观察组采用干扰素栓联合ALA-PDT治疗,观察两组病毒载量变化和HPV清除率、菌群密集度、菌群多样性、优势菌情况、阴道pH变化、Nugnet评分变化以及6个月内复发率。结果观察组治疗后的病毒载量低于对照组,HPV清除率高于对照组,差异有统计学意义(P<0.05);观察组治疗后的菌群密集度正常率、菌群多样性正常率、优势菌群健康率均高于对照组,差异有统计学意义(P<0.05);观察组治疗后的阴道pH、Nugnet评分均低于对照组,差异有统计学意义(P<0.05);观察组6个月内复发率低于对照组,差异有统计学意义(P<0.05)。结论ALA-PDT联合干扰素栓治疗持续高危型HPV感染患者可以提高HPV清除率,改善阴道微环境,降低复发率。

Clinical Effect of Photodynamic Therapy Combined Cryotherapy on Nonmelanoma Skin Cancer

The purpose of this study was to investigate the clinical efficacy of photodynamic combined freezing in patients with non-melanoma skin cancer(NMSC).First,according to the treatment regimen,96 patients with NMSC were divided into study group(n=50)and control group(n=46).The control group was treated with 5-amino-ketovalic acid photodynamic therapy(ALAPDT),while the study group was treated with ala-PDT combined with cryotherapy.Visual analogue scale(VAS)scores,visual satisfaction,clinical efficacy,adverse reactions,and progression-free survival were compared between the two groups.The results showed that VAS score in the study group was slightly higher than that in the control group,but the difference was not statistically significant(P>0.05).The appearance satisfaction and total effective rate of patients in the study group were higher than those in the control group,and the difference was statistically significant(P<0.05).The total incidence of adverse reactions in the study group was slightly higher than that in the control group,but the difference was not statistically significant(P>0.05).3 years progressionfree survival time and 3 years progression-free survival rate were compared between the two groups,and the difference was not statistically significant(P>0.05).Therefore,the combination of PDT and cryotherapy for non-melanoma skin cancer has a good clinical effect,which is conducive to the recovery of skin lesions,high patient satisfaction,fewer adverse reactions,and longer progression-free survival.In addition,the combined therapy can provide a new treatment idea for non-melanoma skin cancer patients who are not suitable for surgical treatment.

5-氨基酮戊酸光动力疗法对中重度痤疮炎症性皮损和炎症后红斑的影响

目的:探讨5-氨基酮戊酸光动力疗法(5-aminolevulinic acid photodynamic therapy,ALA-PDT)治疗中重度痤疮患者的临床疗效。方法:采用随机数字表法将2021年1月-2022年1月笔者医院收治的中重度痤疮患者80例分为对照组(n=40)和治疗组(n=40),对照组予以红蓝光照射治疗,治疗组采取ALA-PDT治疗,均持续治疗4周。比较两组临床疗效,治疗前后痤疮严重程度分级系统评分(Global Acne grading system,GAGS)、痤疮特异性生活质量量表评分(The acne-specific quality of life questionnaire,Acne-QOL)、皮损情况、炎症后红斑情况及不良反应发生情况。结果:治疗组临床总有效率为95.00%,高于对照组的72.50%(P<0.05);与治疗前相比,治疗后两组GAGS评分均降低(P<0.05),两组Acne-QOL量表评分均升高(P<0.05),两组炎性皮损和非炎性皮损数量均减少(P<0.05),两组红斑指数和红斑主观评分均降低(P<0.05),且治疗组GAGS评分、炎性皮损和非炎性皮损个数、红斑指数和红斑主观评分均低于对照组(P<0.05),Acne-QOL量表评分高于对照组(P<0.05);治疗组不良反应总发生率低于对照组(P<0.05)。结论:中重度痤疮患者采用ALA-PDT治疗效果显著,可降低患者痤疮严重程度,提高生活质量,改善皮损情况及炎症后红斑,值得推广。

5-ALA光动力疗法对裸鼠人上皮性卵巢癌移植瘤生长的抑制作用

目的探究5-氨基乙酰丙酸(5-ALA)光动力疗法(PDT)对裸鼠人上皮性卵巢癌移植瘤生长的抑制作用。方法建立裸鼠人上皮性卵巢癌皮下移植瘤模型,分为模型组、5-ALA瘤注组、5-ALA静注组、PDT组、5-ALA瘤注-PDT组、5-ALA静注-PDT组,10只/组。模型组、PDT组给予瘤内注射无菌生理盐水溶液,5-ALA瘤注组、5-ALA瘤注-PDT组给予瘤内注射5-ALA(40 mg/kg)生理盐水溶液,5-ALA静注组、5-ALA静注-PDT组给予尾静脉注射5-ALA(40 mg/kg)生理盐水溶液。1 h避光后,PDT组、5-ALA瘤注-PDT组、5-ALA静注-PDT组用630 nm激光治疗仪局部照射瘤体治疗20 min,每隔2天各组治疗1次,共5次。治疗完成后,安乐处死各组小鼠,肿瘤组织HE染色,测量瘤体体积,采用TUNEL染色法检测肿瘤细胞凋亡率,采用实时定量PCR(qRT-PCR)检测组织cleaved Caspase-3、Bcl-2与Bax的mRNA表达水平,采用Western blot检测组织cleaved Caspase-3、Bcl-2与Bax蛋白表达水平,计算各组的脏器系数。结果相比于模型组、5-ALA瘤注组、5-ALA静注组,PDT组、5-ALA瘤注-PDT组和5-ALA静注-PDT组干预14 d后的瘤体体积均减小,且5-ALA瘤注-PDT组的瘤体体积最小(均P<0.05)。治疗7、14 d后的抑瘤率在PDT组、5-ALA瘤注-PDT组和5-ALA静注-PDT组高于模型组、5-ALA瘤注组、5-ALA静注组(均P<0.05),并且5-ALA瘤注-PDT组的抑瘤率最高(均P<0.05)。组织cleaved Caspase-3、Bax的mRNA与蛋白表达在PDT组、5-ALA瘤注-PDT组和5-ALA静注-PDT组高于模型组、5-ALA瘤注组、5-ALA静注组(均P<0.05),5-ALA瘤注-PDT组的cleaved Caspase-3、Bax的mRNA与蛋白表达量最高(均P<0.05)。组织Bcl-2的mRNA与蛋白表达在PDT组、5-ALA瘤注-PDT组和5-ALA静注-PDT组低于模型组、5-ALA瘤注组、5-ALA静注组(均P<0.05),5-ALA瘤注-PDT组组织Bcl-2的mRNA与蛋白表达水平最低(均P<0.05)。心脏、肝脏、脾脏和肺的脏器系数在PDT组、5-ALA瘤注-PDT组高于模型组、5-ALA瘤注组、5-ALA静注组(均P<0.05),5-ALA静注-PDT组最低(均P<0.05)。结论5-ALA-PDT可抑制皮下移植瘤生长,可能与调节Caspase-3、Bax、Bcl-2相关凋亡蛋白表达相关,瘤注途径较静注途径效果更佳。

5-氨基酮戊酸光动力疗法治愈下唇鳞状细胞癌一例

光动力疗法是一种非侵入性治疗,可在不损害正常组织或细胞的情况下有选择性地消除肿瘤细胞。本文报道一例68岁皮肤鳞状细胞癌患者,经过7次5-氨基酮戊酸光动力治疗后肿瘤完全消退,随访1年无复发。

液氮冷冻联合5-氨基酮戊酸光动力疗法治疗HIV合并肛周尖锐湿疣患者的人乳头瘤病毒载量分析

目的分析液氮冷冻联合5-氨基酮戊酸光动力疗法(ALA-PDT)治疗HIV合并肛周尖锐湿疣(CA)患者的人乳头瘤病毒(HPV)载量情况。方法68例HIV合并肛周CA患者随机分为两组,对照组采用液氮冷冻治疗,观察组采用液氮冷冻联合ALA-PDT治疗。比较两组的临床疗效、复发率以及治疗前后的HPV DNA载量。结果治疗3、6、12个月后,两组的HPV DNA载量均显著降低,且观察组显著低于对照组(P<0.05)。观察组的治愈率显著高于对照组,复发率显著低于对照组(P<0.05)。结论液氮冷冻联合ALA-PDT治疗HIV合并肛周CA患者可有效减少HPV DNA载量,提高疗效,降低复发率。

5-氨基酮戊酸光动力疗法治疗口腔潜在恶性疾患中药物因素影响的分析

目的探讨药物因素对5-氨基酮戊酸(ALA)光动力疗法治疗口腔潜在恶性疾患疗效的影响,以期提高临床疗效及推动临床合理用药。方法检索Pubmed、ISI Web of Knowledge数据库、中国知网、维普以及万方数据库中关于ALA光动力疗法治疗口腔潜在恶性疾患的临床研究,并进行文献分析。结果检索到ALA光动力疗法治疗口腔潜在恶性疾患的临床研究病例共690例,平均完全缓解率为65.94%;16.8%氨基酮戊酸甲酯乳膏组、局部外用组、每周1次组及预激活时间2~4 h组优于其他亚组,平均完全缓解率分别为100%、66.91%、78.28%及77.54%。结论药物因素对5-氨基酮戊酸光动力疗法治疗口腔潜在恶性疾患疗效有直接影响,从药物改良的角度开发新的口腔ALA制剂是提高疗效的重要途径。