In this study, we reported the repaid construction of a molecular marker linkage map of rice (Oryza sativa L.). An F-2 population from the cross between Annong S-1 and Nanjing 11 was used to construct a genetic linkag...In this study, we reported the repaid construction of a molecular marker linkage map of rice (Oryza sativa L.). An F-2 population from the cross between Annong S-1 and Nanjing 11 was used to construct a genetic linkage map of rice. Total of 142 newly screened AFLP markers and 30 anchor markers (25 SSR markers and 5 RFLP markers) were mapped on the 12 chromosomes covering 1537.4 cM of rice genome. The average interval between these markers was 9.0 cM. The total work which usually was finished in more than one year was finished within only 3 months by one person. This is the first plant AFLP map developed in China. A new thermosensitive genic male sterile gene in rice, tms5, was Egged and mapped onto chromosome 2 during the development of the linkage map.展开更多
By making use of the advantages of non-uniform rational B-spline （NURBS） curves to represent spatial curves, an instruction format with double NURBS curves suitable for 5-axis coordinated real-time interpolation is ...By making use of the advantages of non-uniform rational B-spline （NURBS） curves to represent spatial curves, an instruction format with double NURBS curves suitable for 5-axis coordinated real-time interpolation is presented to replace the current 5-axis coordinated linear interpolation method defective in low-speed, low-accuracy and enormous numerical control （NC） files in sculptured surface machining. A generation procedure of the NC files with the presented format is introduced and the method to realize the interpolation in an open computer numerical control （CNC） system is developed by ourselves. These illustrated the feasibility of the proposed method and its capability of avoiding all the shortages of 5-axis linear interpolation method.展开更多
In order to develop a practical postprocessor for 5-axis machine tool,the general equations of numerically controlled(NC) data for 5-axis configurations with non-orthogonal rotary axes were exactly expressed by the in...In order to develop a practical postprocessor for 5-axis machine tool,the general equations of numerically controlled(NC) data for 5-axis configurations with non-orthogonal rotary axes were exactly expressed by the inverse kinematics,and a windows-based postprocessor written with Visual Basic was developed according to the proposed algorithm.The developed postprocessor is a general system suitable for all kinds of 5-axis machines with orthogonal and non-orthogonal rotary axes.Through implementation of the developed postprocessor and verification by a cutting simulation and machining experiment,the effectiveness of the proposed algorithm is confirmed.Compatibility is improved by allowing exchange of data formats such as rotational total center position(RTCP) controlled NC data,vector post NC data,and program object file(POF) cutter location(CL) data,and convenience is increased by adding the function of work-piece origin offset.Consequently,a practical post-processor for 5-axis machining is developed.展开更多
Spatial circular arc can be machined conveniently by a 5-axis NC machine tool.Based on the data sampling method,circular interpolation in two-dimensional plane is discussed briefly.The key is to solve the problem of c...Spatial circular arc can be machined conveniently by a 5-axis NC machine tool.Based on the data sampling method,circular interpolation in two-dimensional plane is discussed briefly.The key is to solve the problem of circular center ex- pressed in the workpiece coordinate system by means of the transformation matrix.Circular interpolation in three-dimensional space is analyzed in detail.The method of undetermined coefficient is used to solve the center of the spatial circle and the method of coor- dinate transformation is used to transform the spatial circle into the XY-plane.Circular arc in three-dimensional space can be ma- chined by the positional 5-axis machining and the conical surface can be machined by the continuous 5-axis machining.The velocity control is presented to avoid the feedrate fluctuation.The interpolation algorithms are tested by a simulation example and the inter- polation algorithms are proved feasible.The algorithms are applied to the 5-axis CNC system software.展开更多
An impeller is difficult to machine because of severe collision due to the complex shape,overlapping and twisted shape of the impeller blades.So,most computer aided manufacturing(CAM)software companies have developed ...An impeller is difficult to machine because of severe collision due to the complex shape,overlapping and twisted shape of the impeller blades.So,most computer aided manufacturing(CAM)software companies have developed CAM module for manufacturing impeller according to their CAM software.But these dedicated modules are difficult to use for inexperienced users.The purpose of this work is to develop a tool-path generation module for impellers.For this purpose,it is based on Visual Basic language and used CATIA graphical environment.The result of simulation for generated tool-path by the module is satisfactory.And it has slow processing speed compared to other commercial modules,but it is easy to use.展开更多
A real-time non-uniform rational B-spline （NURBS） surface interpolator is proposed and 5-axis machining method with a flat-end cutter is discussed. With the Taylor expansion and the coordinate transformation, the al...A real-time non-uniform rational B-spline （NURBS） surface interpolator is proposed and 5-axis machining method with a flat-end cutter is discussed. With the Taylor expansion and the coordinate transformation, the algorithms of NURBS interpolation, cutter effective machining radius, cutter offsetting and.inverse kinematics are deduced and implemented, respectively. Different from the conventional free-form surface machining, the proposed interpolator can real-time generate the motion commands of computer numerical control （CNC） machines with CC feedrate, rather than that of CL. An example part surface is demonstrated and the results of simulation show that the proposed method can be applied in actual 5-axis surface machining.展开更多
In order to estimate the motion errors of 5-axis machine center, the double ball bar （DBB） method is adopted to realize the diagnosis procedure. The motion error sources of rotary axes in 5-axis machining center com...In order to estimate the motion errors of 5-axis machine center, the double ball bar （DBB） method is adopted to realize the diagnosis procedure. The motion error sources of rotary axes in 5-axis machining center comprise of the alignment error of rotary axes and the angular error due to various factors, e.g. the inclination of rotary axes. From sensitive viewpoints, each motion error is possible to have a particular sensitive direction in which deviation of DBB error trace arises from only some specific error sources. The model of the DBB error trace is established according to the spatial geometry theory. Accordingly, the sensitive direction of each motion error source is made clear through numerical simulation, which is used as the reference patterns for rotational error estimation. The estimation method is proposed to easily estimate the motion error sources of rotary axes in quantitative manner. To verify the proposed DBB method for rotational error estimation, the experimental tests are carried out on a 5-axis machining center M-400 （MORISEIKI）. The effect of the mismatch of the DBB is also studied to guarantee the estimation accuracy. From the experimental data, it is noted that the proposed estimation method for 5-axis machining center is feasible and effective.展开更多
It has been reported that there was a linkage of 5S rRNA gene to 18S-5.8S-25S rRNA gene in a few of species in Ochrophyta.In regard to the usual two positions of linked 5S rDNA to the 3′end of 25S rDNA,two pairs of p...It has been reported that there was a linkage of 5S rRNA gene to 18S-5.8S-25S rRNA gene in a few of species in Ochrophyta.In regard to the usual two positions of linked 5S rDNA to the 3′end of 25S rDNA,two pairs of primers were designed for amplification to verify this linkage of two genes in a kelp cultivar of Saccharina japonica,one of species in Ochrophyta.This result supplemented the previous report that 5S rDNA was unlinked to 25S rDNA in this kelp.In order to simultaneously visualize this unlinkage of two genes,dual-color fluorescence in situ hybridization(FISH)technique was applied to the cytogenetics of S.japonica.Dual-color FISH images showed that two and four hybridization signals were present in the kelp gametophyte and sporophyte,respectively,metaphase nuclei hybridized simultaneously with the labeled probes of 18S rDNA and 5S rDNA.Both haploid and diploid karyotypes in decreasing length of chromosomes showed that 18S-5.8S-25S rDNA was localized at the interstitial region of Chromosome 23,whereas 5S rDNA resided at the sub-telomeric region of Chromosome 27.These karyotypes suggested that the kelp nuclear genome had only one locus of each rRNA gene,and their loci on different chromosomes indicated the physical unlinkage of 5S rDNA to 18S-5.8S-25S rDNA in this kelp.Therefore,dual-color FISH seems to be a powerful technique for the discrimination and pairing of chromosomes featured in both small size and nearly identical shape in S.japonica.展开更多
The problem of finished surface being not first-order continuous commonly exists in machining sculptured surfaces with a torus cutter and some other types of cutters. To solve this problem, a dual drive curve tool pat...The problem of finished surface being not first-order continuous commonly exists in machining sculptured surfaces with a torus cutter and some other types of cutters. To solve this problem, a dual drive curve tool path planning method is proposed in this article. First, the maximum machining strip width of a whole tool path can be obtained through optimizing each tool position with multi-point machining （MPM） method. Second, two drive curves are then determined according to the obtained maximum machining strip width. Finally, the tool is positioned once more along the dual drive curve under the condition of tool path smoothness. A computer simulation and cutting experiments are carried out to testify the performance of the new method. The machined surface is measured with a coordinate measuring machine （CMM） to examine the machining quality. The results obtained show that this method can effectively eliminate sharp scallops between adjacent tool paths, keep tool paths smooth, and improve the surface machining quality as well as machining efficiency.展开更多
A 5-axis controller with curve interpolation function is developed to satisfy high-speed and high-precision computer numerical control (CNC) machining of machine parts with complex shapes in the authors-devised open C...A 5-axis controller with curve interpolation function is developed to satisfy high-speed and high-precision computer numerical control (CNC) machining of machine parts with complex shapes in the authors-devised open CNC system. The instruction for- mat of this interpolation method and the generation procedure of the numerical control (NC) files are introduced. The interpola- tion curves of both position vectors and orientation vectors constructed by the controller are C2 continuous and independent of machin...展开更多
文摘In this study, we reported the repaid construction of a molecular marker linkage map of rice (Oryza sativa L.). An F-2 population from the cross between Annong S-1 and Nanjing 11 was used to construct a genetic linkage map of rice. Total of 142 newly screened AFLP markers and 30 anchor markers (25 SSR markers and 5 RFLP markers) were mapped on the 12 chromosomes covering 1537.4 cM of rice genome. The average interval between these markers was 9.0 cM. The total work which usually was finished in more than one year was finished within only 3 months by one person. This is the first plant AFLP map developed in China. A new thermosensitive genic male sterile gene in rice, tms5, was Egged and mapped onto chromosome 2 during the development of the linkage map.
文摘By making use of the advantages of non-uniform rational B-spline （NURBS） curves to represent spatial curves, an instruction format with double NURBS curves suitable for 5-axis coordinated real-time interpolation is presented to replace the current 5-axis coordinated linear interpolation method defective in low-speed, low-accuracy and enormous numerical control （NC） files in sculptured surface machining. A generation procedure of the NC files with the presented format is introduced and the method to realize the interpolation in an open computer numerical control （CNC） system is developed by ourselves. These illustrated the feasibility of the proposed method and its capability of avoiding all the shortages of 5-axis linear interpolation method.
基金Work supported by the Second Stage of Brain Korea 21 Projects
文摘In order to develop a practical postprocessor for 5-axis machine tool,the general equations of numerically controlled(NC) data for 5-axis configurations with non-orthogonal rotary axes were exactly expressed by the inverse kinematics,and a windows-based postprocessor written with Visual Basic was developed according to the proposed algorithm.The developed postprocessor is a general system suitable for all kinds of 5-axis machines with orthogonal and non-orthogonal rotary axes.Through implementation of the developed postprocessor and verification by a cutting simulation and machining experiment,the effectiveness of the proposed algorithm is confirmed.Compatibility is improved by allowing exchange of data formats such as rotational total center position(RTCP) controlled NC data,vector post NC data,and program object file(POF) cutter location(CL) data,and convenience is increased by adding the function of work-piece origin offset.Consequently,a practical post-processor for 5-axis machining is developed.
文摘Spatial circular arc can be machined conveniently by a 5-axis NC machine tool.Based on the data sampling method,circular interpolation in two-dimensional plane is discussed briefly.The key is to solve the problem of circular center ex- pressed in the workpiece coordinate system by means of the transformation matrix.Circular interpolation in three-dimensional space is analyzed in detail.The method of undetermined coefficient is used to solve the center of the spatial circle and the method of coor- dinate transformation is used to transform the spatial circle into the XY-plane.Circular arc in three-dimensional space can be ma- chined by the positional 5-axis machining and the conical surface can be machined by the continuous 5-axis machining.The velocity control is presented to avoid the feedrate fluctuation.The interpolation algorithms are tested by a simulation example and the inter- polation algorithms are proved feasible.The algorithms are applied to the 5-axis CNC system software.
基金Project supported by the Second Stage of Brain Korea 21 Projects
文摘An impeller is difficult to machine because of severe collision due to the complex shape,overlapping and twisted shape of the impeller blades.So,most computer aided manufacturing(CAM)software companies have developed CAM module for manufacturing impeller according to their CAM software.But these dedicated modules are difficult to use for inexperienced users.The purpose of this work is to develop a tool-path generation module for impellers.For this purpose,it is based on Visual Basic language and used CATIA graphical environment.The result of simulation for generated tool-path by the module is satisfactory.And it has slow processing speed compared to other commercial modules,but it is easy to use.
文摘A real-time non-uniform rational B-spline （NURBS） surface interpolator is proposed and 5-axis machining method with a flat-end cutter is discussed. With the Taylor expansion and the coordinate transformation, the algorithms of NURBS interpolation, cutter effective machining radius, cutter offsetting and.inverse kinematics are deduced and implemented, respectively. Different from the conventional free-form surface machining, the proposed interpolator can real-time generate the motion commands of computer numerical control （CNC） machines with CC feedrate, rather than that of CL. An example part surface is demonstrated and the results of simulation show that the proposed method can be applied in actual 5-axis surface machining.
文摘In order to estimate the motion errors of 5-axis machine center, the double ball bar （DBB） method is adopted to realize the diagnosis procedure. The motion error sources of rotary axes in 5-axis machining center comprise of the alignment error of rotary axes and the angular error due to various factors, e.g. the inclination of rotary axes. From sensitive viewpoints, each motion error is possible to have a particular sensitive direction in which deviation of DBB error trace arises from only some specific error sources. The model of the DBB error trace is established according to the spatial geometry theory. Accordingly, the sensitive direction of each motion error source is made clear through numerical simulation, which is used as the reference patterns for rotational error estimation. The estimation method is proposed to easily estimate the motion error sources of rotary axes in quantitative manner. To verify the proposed DBB method for rotational error estimation, the experimental tests are carried out on a 5-axis machining center M-400 （MORISEIKI）. The effect of the mismatch of the DBB is also studied to guarantee the estimation accuracy. From the experimental data, it is noted that the proposed estimation method for 5-axis machining center is feasible and effective.
基金Supported by the National Natural Science Foundation of China(Nos.41376136,31201992)the Double First-Class Discipline of Fisheries Science in China。
文摘It has been reported that there was a linkage of 5S rRNA gene to 18S-5.8S-25S rRNA gene in a few of species in Ochrophyta.In regard to the usual two positions of linked 5S rDNA to the 3′end of 25S rDNA,two pairs of primers were designed for amplification to verify this linkage of two genes in a kelp cultivar of Saccharina japonica,one of species in Ochrophyta.This result supplemented the previous report that 5S rDNA was unlinked to 25S rDNA in this kelp.In order to simultaneously visualize this unlinkage of two genes,dual-color fluorescence in situ hybridization(FISH)technique was applied to the cytogenetics of S.japonica.Dual-color FISH images showed that two and four hybridization signals were present in the kelp gametophyte and sporophyte,respectively,metaphase nuclei hybridized simultaneously with the labeled probes of 18S rDNA and 5S rDNA.Both haploid and diploid karyotypes in decreasing length of chromosomes showed that 18S-5.8S-25S rDNA was localized at the interstitial region of Chromosome 23,whereas 5S rDNA resided at the sub-telomeric region of Chromosome 27.These karyotypes suggested that the kelp nuclear genome had only one locus of each rRNA gene,and their loci on different chromosomes indicated the physical unlinkage of 5S rDNA to 18S-5.8S-25S rDNA in this kelp.Therefore,dual-color FISH seems to be a powerful technique for the discrimination and pairing of chromosomes featured in both small size and nearly identical shape in S.japonica.
基金National Natural Science Foundation of China (50875012)National High-tech Research and Development Program (2008AA04Z124)+1 种基金National Science and Technology Major Project (2009ZX04001-141)Joint Construction Project of Beijing Municipal Commission of Education
文摘The problem of finished surface being not first-order continuous commonly exists in machining sculptured surfaces with a torus cutter and some other types of cutters. To solve this problem, a dual drive curve tool path planning method is proposed in this article. First, the maximum machining strip width of a whole tool path can be obtained through optimizing each tool position with multi-point machining （MPM） method. Second, two drive curves are then determined according to the obtained maximum machining strip width. Finally, the tool is positioned once more along the dual drive curve under the condition of tool path smoothness. A computer simulation and cutting experiments are carried out to testify the performance of the new method. The machined surface is measured with a coordinate measuring machine （CMM） to examine the machining quality. The results obtained show that this method can effectively eliminate sharp scallops between adjacent tool paths, keep tool paths smooth, and improve the surface machining quality as well as machining efficiency.
基金Key Development Program of Science and Technology of Heilongjiang Province, China (GB05A501)
文摘A 5-axis controller with curve interpolation function is developed to satisfy high-speed and high-precision computer numerical control (CNC) machining of machine parts with complex shapes in the authors-devised open CNC system. The instruction for- mat of this interpolation method and the generation procedure of the numerical control (NC) files are introduced. The interpola- tion curves of both position vectors and orientation vectors constructed by the controller are C2 continuous and independent of machin...
