目的探讨甲基转移酶5(methyltransferase-like 5,METTL5)在三阴乳腺癌(triple-negative breast cancer,TNBC)中的作用和潜在机制。方法采用免疫组织化学方法和Western blot检测TNBC肿瘤组织和细胞系中METTL5的表达情况。用靶向METTL5的s...目的探讨甲基转移酶5(methyltransferase-like 5,METTL5)在三阴乳腺癌(triple-negative breast cancer,TNBC)中的作用和潜在机制。方法采用免疫组织化学方法和Western blot检测TNBC肿瘤组织和细胞系中METTL5的表达情况。用靶向METTL5的shRNA(shRNA-METTL5)转染TNBC细胞后,用CCK-8、集落形成、伤口愈合以及Transwell实验分别检测细胞增殖活性、迁移与侵袭,Western blot检测Wnt/β-catenin信号关键蛋白的表达。构建异种移植瘤模型,验证敲降METTL5对TNBC细胞在体内生长以及Wnt/β-catenin信号活性的影响。结果METTL5在TNBC肿瘤组织和细胞系中表达上调(P<0.01)。敲降METTL5可抑制TNBC细胞的增殖、迁移和侵袭并降低了Wnt/β-catenin信号分子β-catenin、细胞周期蛋白(Cyclin)D1、基质金属蛋白酶(MMP)-2和MMP-7的表达(均P<0.01)。体内实验显示,敲降METTL5减缓了移植瘤生长和Wnt/β-catenin信号活性。结论敲降METTL5能抑制TNBC细胞的增殖、迁移与侵袭,其作用可能与抑制Wnt/β-catenin信号通路有关。展开更多
A novel energetic combustion catalyst, 4-amino-3,S-dinitropyrazole copper salt ([Cu(adnp)2(H2O)2]), was synthesized in a yield of 93.6% for the first time. The single crystal of [Cu(adnp)2(H2O)2] was determi...A novel energetic combustion catalyst, 4-amino-3,S-dinitropyrazole copper salt ([Cu(adnp)2(H2O)2]), was synthesized in a yield of 93.6% for the first time. The single crystal of [Cu(adnp)2(H2O)2] was determined by single crystal X-ray diffraction. It crystallizes in a triclinic system, space group p^-1 with crystal parameters a = 5.541(3) A, b = 7.926(4) A, c = 10.231(5) A,β = 101.372(8)°, V = 398.3(3) A3, Z = 1, μ = 1.467 mm^-1, F(0 0 0) = 243, and Dc = 2.000 g cm^-3. The thermal behavior and non-isothermal decomposition reaction kinetics of [Cu(adnp)2(H2O)2] were studied by means of different heating rate differential scanning calorimetry (DSC). The kinetic equation of major exothermic decomposition reaction for [Cu(adnp)2(H2O)2] was obtained. The entropy of activation (△S≠), enthalpy of activation (△H≠), free energy of activation (△G≠), the self-accelerating decomposition temperature (TSADT) and the critical temperature of thermal explosion (Tb) are 59.42 j mol^-1 K^-1, 169.5 kJ mol^-1, 1141.26 kJ mol ^-1 457.3 K and 468.1 K, respectively.展开更多
基金the National Science Foundation of China(No.21173163 and No. 21303133)
文摘A novel energetic combustion catalyst, 4-amino-3,S-dinitropyrazole copper salt ([Cu(adnp)2(H2O)2]), was synthesized in a yield of 93.6% for the first time. The single crystal of [Cu(adnp)2(H2O)2] was determined by single crystal X-ray diffraction. It crystallizes in a triclinic system, space group p^-1 with crystal parameters a = 5.541(3) A, b = 7.926(4) A, c = 10.231(5) A,β = 101.372(8)°, V = 398.3(3) A3, Z = 1, μ = 1.467 mm^-1, F(0 0 0) = 243, and Dc = 2.000 g cm^-3. The thermal behavior and non-isothermal decomposition reaction kinetics of [Cu(adnp)2(H2O)2] were studied by means of different heating rate differential scanning calorimetry (DSC). The kinetic equation of major exothermic decomposition reaction for [Cu(adnp)2(H2O)2] was obtained. The entropy of activation (△S≠), enthalpy of activation (△H≠), free energy of activation (△G≠), the self-accelerating decomposition temperature (TSADT) and the critical temperature of thermal explosion (Tb) are 59.42 j mol^-1 K^-1, 169.5 kJ mol^-1, 1141.26 kJ mol ^-1 457.3 K and 468.1 K, respectively.