Objective：To determine the effects of 5-hydroxymethyl furfural（5-HMF）,an extract of Rehmannia glutinosa Libosch,on several down-regulated signaling molecules involved in learning and memory in hippocampal neurons.M...Objective：To determine the effects of 5-hydroxymethyl furfural（5-HMF）,an extract of Rehmannia glutinosa Libosch,on several down-regulated signaling molecules involved in learning and memory in hippocampal neurons.Methods：After cultured for 7 days,primary hippocampal neurons were divided into 5 groups：normal,corticosterone model,RU38486,5-HMF,and donepezil group.Neuron survival rates were calculated 24 h later using SYT013-P1 double-fluorescence staining and an 3-（4,5-Dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT）assay.β-galactosidase activity was also assayed.Protein expressed by the glucocorticoid receptor（GCR）,brainderived neurotrophic factor（BDNF）,and N-methyl-D-aspartate receptor 2B（NR2B）,as well as phosphorylationcyclic adenosine monophosphate（cAMP）response element binding protein（p-CREB）,phosphorylation-extracellular signal-regulated kinase（p-ERK）,and phosphorylation-synapsin（p-synapsin）were quantified with Western blot.Results：Hippocampal neuron survival rates and the above-mentioned proteins were dramatically decreased（P〈0.05）,β-galactosidase activity was significantly increased in the model group,but the effect was reversed by5-HMF,RU38486,and to a lesser extent by donepezil（P〈0.05）.Conclusion：5-HMF extracts from the Chinese herb Rehmannia glutinosa Libosch could protect hippocampal neurons from glucocorticoid injury and from down-regulated signaling molecules in the GCR-BDNF-NR2B-p-ERK-p-CREB-p-synapsin signal transduction pathway.展开更多
目的：建立测定九味羌活丸中5-羟甲基糠醛含量的方法。方法：采用高效液相色谱法。色谱柱为Phenomenex Luna C18,流动相为甲醇-0.1%磷酸溶液（3∶97,V/V）,流速为1 ml/min,柱温为25℃,进样量为5μl,检测波长为280nm。结果：5-羟甲基糠...目的：建立测定九味羌活丸中5-羟甲基糠醛含量的方法。方法：采用高效液相色谱法。色谱柱为Phenomenex Luna C18,流动相为甲醇-0.1%磷酸溶液（3∶97,V/V）,流速为1 ml/min,柱温为25℃,进样量为5μl,检测波长为280nm。结果：5-羟甲基糠醛进样量在0.066 64-0.333 2μg范围内与峰面积积分值呈良好的线性关系（r=0.999 2）;精密度、稳定性、重复性试验的RSD≤1.15%;平均加样回收率为100.84%,RSD=4.06%（n=6）。结论：该方法简便、快速,重复性好,可用于九味羌活丸中5-羟甲基糠醛的含量测定。展开更多
基金Support by the National Natural Science Foundation of China(No.30873317),Leading Academic Discipline Project of Shanghai Municipal Education Commission(No.J50301),Shanghai Health Bureau of Scientific Research Projects(No.20124Y025)
文摘Objective：To determine the effects of 5-hydroxymethyl furfural（5-HMF）,an extract of Rehmannia glutinosa Libosch,on several down-regulated signaling molecules involved in learning and memory in hippocampal neurons.Methods：After cultured for 7 days,primary hippocampal neurons were divided into 5 groups：normal,corticosterone model,RU38486,5-HMF,and donepezil group.Neuron survival rates were calculated 24 h later using SYT013-P1 double-fluorescence staining and an 3-（4,5-Dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT）assay.β-galactosidase activity was also assayed.Protein expressed by the glucocorticoid receptor（GCR）,brainderived neurotrophic factor（BDNF）,and N-methyl-D-aspartate receptor 2B（NR2B）,as well as phosphorylationcyclic adenosine monophosphate（cAMP）response element binding protein（p-CREB）,phosphorylation-extracellular signal-regulated kinase（p-ERK）,and phosphorylation-synapsin（p-synapsin）were quantified with Western blot.Results：Hippocampal neuron survival rates and the above-mentioned proteins were dramatically decreased（P〈0.05）,β-galactosidase activity was significantly increased in the model group,but the effect was reversed by5-HMF,RU38486,and to a lesser extent by donepezil（P〈0.05）.Conclusion：5-HMF extracts from the Chinese herb Rehmannia glutinosa Libosch could protect hippocampal neurons from glucocorticoid injury and from down-regulated signaling molecules in the GCR-BDNF-NR2B-p-ERK-p-CREB-p-synapsin signal transduction pathway.
