目的探讨小鼠心肌梗死进程中巨噬细胞亚群的动态变化与CD59、P2X7表达的动态变化。方法将成年雄性C57BL/6J小鼠36只随机分为对照组(不做任何处理,6只)和心肌梗死组(30只)。通过冠状动脉左前降支结扎建立小鼠急性心肌梗死模型,心肌梗死...目的探讨小鼠心肌梗死进程中巨噬细胞亚群的动态变化与CD59、P2X7表达的动态变化。方法将成年雄性C57BL/6J小鼠36只随机分为对照组(不做任何处理,6只)和心肌梗死组(30只)。通过冠状动脉左前降支结扎建立小鼠急性心肌梗死模型,心肌梗死组30只小鼠又随机分为A、B、C、D、E组,分别在心肌梗死后1、3、5、7、14 d取材。收取小鼠的外周血和心脏组织,采用流式细胞术检测巨噬细胞亚群的动态变化与CD59和P2X7的表达情况。结果心肌梗死后1、3 d外周血中单核细胞数量均高于对照组[(9.5±1.7)、(10.3±3.7)×10^(4)/ml比(3.2±3.1)×10^(4)/ml](均P<0.05)。心肌梗死后1、3、5 d心脏组织白细胞计数、巨噬细胞计数均高于对照组(均P<0.05)。心脏组织中Ly6C^(high)巨噬细胞计数在心肌梗死后3 d达到高峰,而Ly6C^(low)亚群在心肌梗死后5 d达到高峰,均高于对照组(均P<0.05)。心肌梗死后1 d P2X7^(-)CD59^(-)巨噬细胞占Ly6C^(high)巨噬细胞百分比高于对照组,而P2X7^(+)CD59^(+)巨噬细胞占比低于对照组(均P<0.05)。心肌梗死后1、3、5 d P2X7^(+)CD59^(+)巨噬细胞占Ly6C^(low)巨噬细胞百分比均低于对照组,而P2X7^(-)CD59^(+)巨噬细胞占比均高于对照组(均P<0.05)。结论在小鼠急性心肌梗死的不同阶段,心脏组织巨噬细胞的亚群呈现动态变化,同时伴随CD59和P2X7表达的动态变化。展开更多
目的:探讨干扰三元基序59(TRIM59)表达对慢性粒细胞白血病K562细胞柔红霉素(DNR)化疗敏感性的影响及相关分子机制。方法:采用RT-q PCR法检测慢性粒细胞白血病患者骨髓组织和K562细胞中TRIM59 m RNA表达水平。用脂质体转染法将TRIM59特...目的:探讨干扰三元基序59(TRIM59)表达对慢性粒细胞白血病K562细胞柔红霉素(DNR)化疗敏感性的影响及相关分子机制。方法:采用RT-q PCR法检测慢性粒细胞白血病患者骨髓组织和K562细胞中TRIM59 m RNA表达水平。用脂质体转染法将TRIM59特异性小干扰RNA(si-TRIM59)转染至K562细胞,并用DNR处理细胞。CCK-8法检测细胞增殖,流式细胞术检测细胞凋亡,Western blot法检测凋亡相关蛋白和Wnt/β-catenin信号通路相关蛋白表达。结果:与初治时骨髓组织相比,化疗耐药时患者骨髓组织中TRIM59 m RNA表达水平升高(P<0.05)。与对照组比较,si-TRIM59组和DNR组细胞增殖抑制率、细胞凋亡率均显著升高(P<0.05);细胞中Bax、Caspase3、Cleaved-Caspase3蛋白表达量均显著升高,而Bcl-2、Wnt3α、GSK-3β蛋白表达量、p-β-catenin/β-catenin比值均显著降低(P<0.05)。与si-TRIM59组和DNR组比较,si-TRIM59+DNR组细胞增殖抑制率、细胞凋亡率均显著升高(P<0.05);细胞中Bax、Caspase3、Cleaved-Caspase3蛋白表达量均显著升高,而Bcl-2、Wnt3α、GSK-3β蛋白表达量、p-β-catenin/β-catenin比值均显著降低(P<0.05)。结论:干扰TRIM59表达可增强K562细胞对DNR的化疗敏感性,其作用机制可能与调控Wnt/β-catenin信号通路相关。展开更多
Malnutrition is a major public health concern in developing countries,contributing to 45%of deaths among children under five years old.In the Republic of Guinea,the prevalence of acute malnutrition is estimated at 6.7...Malnutrition is a major public health concern in developing countries,contributing to 45%of deaths among children under five years old.In the Republic of Guinea,the prevalence of acute malnutrition is estimated at 6.7%,largely influenced by inadequate breastfeeding and complementary feeding practices.A descriptive cross-sectional study was conducted in the Prefecture of Fria to assess the impact of feeding practices on the growth and development of children aged 6 to 59 months.A sample of 450 children residing in the prefecture was included in the study.The findings revealed a prevalence of acute malnutrition of 8.9%,while 4.4%of the children were underweight,and 7.1%suffered from chronic malnutrition.展开更多
Introduction: Malnutrition is an important reason for consultation in Mali’s health facilities and remains a major public health problem. The aim of this study was to describe the epidemioclinical profile and associa...Introduction: Malnutrition is an important reason for consultation in Mali’s health facilities and remains a major public health problem. The aim of this study was to describe the epidemioclinical profile and associated factors with performance indicators of integrated management of severe acute malnutrition in children aged 06 to 59 months. Methodology: this was a cross-sectional study with retrospective data collection (January 2021 to December 2022). All children hospitalized for severe acute malnutrition in the pediatric department and whose medical records were usable were included. Data collected using a standardized questionnaire was analyzed with SPSS Version 20 software. Results: A total of 534 children were included. The 12 to 23 months age group (49.1%) and the female sex (53.18%) were the most affected. Fully vaccinated children by age represented 49.4%. The predominant form of malnutrition was marasmus (77.7%). Diarrhea/vomiting (30.3%), fever (18.4%) and cough (15.5%) were the main reasons for consultations. Cure, discontinuation and death rates were 78.5%, 2.1% and 9.2%, respectively. On univariate analysis, the factors statistically associated with performance indicators (cure, drop-out, death) were gastroenteritis (P-value Conclusion: This study reveals that the frequency of severe acute malnutrition remains high at the Kalaban Coro reference health center. Better prevention of illnesses such as malaria, gastroenteritis, and respiratory infections, as well as timely referral, could help facilitate its management.展开更多
Introduction: the problem of Severe Acute Malnutrition (SAM) and its consequences concern and challenge all social actors worldwide. The objective of the study was to identify the real obstacles that actors face in th...Introduction: the problem of Severe Acute Malnutrition (SAM) and its consequences concern and challenge all social actors worldwide. The objective of the study was to identify the real obstacles that actors face in the integrated management of children aged 6 - 59 months suffering from SAM at the University Hospital of Mother and Child (CHUME) and the Chad-China Friendship Hospital (HATC) of N’Djamena. Methodology: this is a cross-sectional descriptive and interpretative study conducted from January to October 2023 at the CHUME and HATC of N’Djamena. It is based on questionnaires and direct observation of two hundred and thirty-six (236) parents or guardians of malnourished children admitted to these health facilities. All 236 agreed to participate freely in this study. Results: it appears from this study that the obstacles to the integrated management of malnourished children were: poor care-care relationship (26.7%) permanent product breaks (22.9%), the direct costs of services ranged from 30,000 to 60,000 FCFA (41.53%), drugs used for the treatment of patients came from the street (66.10%), malaria and anemia occupy an important place (27.5%), 57.2% respondents had monthly income below 60,000 CFAF. Conclusion: the present study carried out has identified the real bottlenecks to the integrated management of children suffering from SAM in the therapeutic nutritional units of the city of N’Djamena. .展开更多
目的:研究CD59分子在LAT(Linker for activated T cells)介导的T系淋巴细胞活化中所起的作用。方法:构建LAT-GFP融合蛋白,以逆转录病毒为载体转染Jurkat细胞,建立稳定转染细胞株(Jurkat-GFP)。分别使用CD59抗体刺激和pSUPER-siCD59干扰...目的:研究CD59分子在LAT(Linker for activated T cells)介导的T系淋巴细胞活化中所起的作用。方法:构建LAT-GFP融合蛋白,以逆转录病毒为载体转染Jurkat细胞,建立稳定转染细胞株(Jurkat-GFP)。分别使用CD59抗体刺激和pSUPER-siCD59干扰质粒(GFP标记)沉默Jurkat-GFP细胞。免疫荧光观察CD59和LAT分子在细胞膜的表达和定位;MTT比色法检测细胞增殖率的变化;Western blot检测LAT活化信号转导通路中相关蛋白分子磷酸化水平。结果:荧光显微镜下可见Jurkat-GFP细胞绿色荧光表达于细胞膜,转染干扰质粒后细胞膜和细胞质均有绿色荧光表达。激光共聚焦显微镜下可见CD59抗体刺激前CD59和LAT分子均匀分布于细胞膜,且转染干扰质粒的Jurkat-GFP细胞CD59表达量下降。CD59抗体刺激后CD59分子和LAT分子点状聚集共定位于细胞膜。MTT结果表明相对于正常Jurkat-GFP细胞,抗体活化后细胞增殖速率明显升高(P>0.05),而电转干扰质粒后细胞生长减慢。Western blot结果表明,CD59抗体刺激后细胞ZAP-70、LCK、PLC-r总蛋白表达无明显差异(P>0.05),而磷酸化蛋白表达显著增高(P<0.05)。结论:抗体活化后细胞膜上CD59和LAT分子聚集并共定位于细胞膜,且细胞信号转导通路下游分子磷酸化水平增高,进一步证实CD59分子经抗体活化后可促进LAT介导的T细胞信号转导。展开更多
文摘目的探讨小鼠心肌梗死进程中巨噬细胞亚群的动态变化与CD59、P2X7表达的动态变化。方法将成年雄性C57BL/6J小鼠36只随机分为对照组(不做任何处理,6只)和心肌梗死组(30只)。通过冠状动脉左前降支结扎建立小鼠急性心肌梗死模型,心肌梗死组30只小鼠又随机分为A、B、C、D、E组,分别在心肌梗死后1、3、5、7、14 d取材。收取小鼠的外周血和心脏组织,采用流式细胞术检测巨噬细胞亚群的动态变化与CD59和P2X7的表达情况。结果心肌梗死后1、3 d外周血中单核细胞数量均高于对照组[(9.5±1.7)、(10.3±3.7)×10^(4)/ml比(3.2±3.1)×10^(4)/ml](均P<0.05)。心肌梗死后1、3、5 d心脏组织白细胞计数、巨噬细胞计数均高于对照组(均P<0.05)。心脏组织中Ly6C^(high)巨噬细胞计数在心肌梗死后3 d达到高峰,而Ly6C^(low)亚群在心肌梗死后5 d达到高峰,均高于对照组(均P<0.05)。心肌梗死后1 d P2X7^(-)CD59^(-)巨噬细胞占Ly6C^(high)巨噬细胞百分比高于对照组,而P2X7^(+)CD59^(+)巨噬细胞占比低于对照组(均P<0.05)。心肌梗死后1、3、5 d P2X7^(+)CD59^(+)巨噬细胞占Ly6C^(low)巨噬细胞百分比均低于对照组,而P2X7^(-)CD59^(+)巨噬细胞占比均高于对照组(均P<0.05)。结论在小鼠急性心肌梗死的不同阶段,心脏组织巨噬细胞的亚群呈现动态变化,同时伴随CD59和P2X7表达的动态变化。
文摘目的:探讨干扰三元基序59(TRIM59)表达对慢性粒细胞白血病K562细胞柔红霉素(DNR)化疗敏感性的影响及相关分子机制。方法:采用RT-q PCR法检测慢性粒细胞白血病患者骨髓组织和K562细胞中TRIM59 m RNA表达水平。用脂质体转染法将TRIM59特异性小干扰RNA(si-TRIM59)转染至K562细胞,并用DNR处理细胞。CCK-8法检测细胞增殖,流式细胞术检测细胞凋亡,Western blot法检测凋亡相关蛋白和Wnt/β-catenin信号通路相关蛋白表达。结果:与初治时骨髓组织相比,化疗耐药时患者骨髓组织中TRIM59 m RNA表达水平升高(P<0.05)。与对照组比较,si-TRIM59组和DNR组细胞增殖抑制率、细胞凋亡率均显著升高(P<0.05);细胞中Bax、Caspase3、Cleaved-Caspase3蛋白表达量均显著升高,而Bcl-2、Wnt3α、GSK-3β蛋白表达量、p-β-catenin/β-catenin比值均显著降低(P<0.05)。与si-TRIM59组和DNR组比较,si-TRIM59+DNR组细胞增殖抑制率、细胞凋亡率均显著升高(P<0.05);细胞中Bax、Caspase3、Cleaved-Caspase3蛋白表达量均显著升高,而Bcl-2、Wnt3α、GSK-3β蛋白表达量、p-β-catenin/β-catenin比值均显著降低(P<0.05)。结论:干扰TRIM59表达可增强K562细胞对DNR的化疗敏感性,其作用机制可能与调控Wnt/β-catenin信号通路相关。
文摘Malnutrition is a major public health concern in developing countries,contributing to 45%of deaths among children under five years old.In the Republic of Guinea,the prevalence of acute malnutrition is estimated at 6.7%,largely influenced by inadequate breastfeeding and complementary feeding practices.A descriptive cross-sectional study was conducted in the Prefecture of Fria to assess the impact of feeding practices on the growth and development of children aged 6 to 59 months.A sample of 450 children residing in the prefecture was included in the study.The findings revealed a prevalence of acute malnutrition of 8.9%,while 4.4%of the children were underweight,and 7.1%suffered from chronic malnutrition.
文摘Introduction: Malnutrition is an important reason for consultation in Mali’s health facilities and remains a major public health problem. The aim of this study was to describe the epidemioclinical profile and associated factors with performance indicators of integrated management of severe acute malnutrition in children aged 06 to 59 months. Methodology: this was a cross-sectional study with retrospective data collection (January 2021 to December 2022). All children hospitalized for severe acute malnutrition in the pediatric department and whose medical records were usable were included. Data collected using a standardized questionnaire was analyzed with SPSS Version 20 software. Results: A total of 534 children were included. The 12 to 23 months age group (49.1%) and the female sex (53.18%) were the most affected. Fully vaccinated children by age represented 49.4%. The predominant form of malnutrition was marasmus (77.7%). Diarrhea/vomiting (30.3%), fever (18.4%) and cough (15.5%) were the main reasons for consultations. Cure, discontinuation and death rates were 78.5%, 2.1% and 9.2%, respectively. On univariate analysis, the factors statistically associated with performance indicators (cure, drop-out, death) were gastroenteritis (P-value Conclusion: This study reveals that the frequency of severe acute malnutrition remains high at the Kalaban Coro reference health center. Better prevention of illnesses such as malaria, gastroenteritis, and respiratory infections, as well as timely referral, could help facilitate its management.
文摘Introduction: the problem of Severe Acute Malnutrition (SAM) and its consequences concern and challenge all social actors worldwide. The objective of the study was to identify the real obstacles that actors face in the integrated management of children aged 6 - 59 months suffering from SAM at the University Hospital of Mother and Child (CHUME) and the Chad-China Friendship Hospital (HATC) of N’Djamena. Methodology: this is a cross-sectional descriptive and interpretative study conducted from January to October 2023 at the CHUME and HATC of N’Djamena. It is based on questionnaires and direct observation of two hundred and thirty-six (236) parents or guardians of malnourished children admitted to these health facilities. All 236 agreed to participate freely in this study. Results: it appears from this study that the obstacles to the integrated management of malnourished children were: poor care-care relationship (26.7%) permanent product breaks (22.9%), the direct costs of services ranged from 30,000 to 60,000 FCFA (41.53%), drugs used for the treatment of patients came from the street (66.10%), malaria and anemia occupy an important place (27.5%), 57.2% respondents had monthly income below 60,000 CFAF. Conclusion: the present study carried out has identified the real bottlenecks to the integrated management of children suffering from SAM in the therapeutic nutritional units of the city of N’Djamena. .
文摘目的:研究CD59分子在LAT(Linker for activated T cells)介导的T系淋巴细胞活化中所起的作用。方法:构建LAT-GFP融合蛋白,以逆转录病毒为载体转染Jurkat细胞,建立稳定转染细胞株(Jurkat-GFP)。分别使用CD59抗体刺激和pSUPER-siCD59干扰质粒(GFP标记)沉默Jurkat-GFP细胞。免疫荧光观察CD59和LAT分子在细胞膜的表达和定位;MTT比色法检测细胞增殖率的变化;Western blot检测LAT活化信号转导通路中相关蛋白分子磷酸化水平。结果:荧光显微镜下可见Jurkat-GFP细胞绿色荧光表达于细胞膜,转染干扰质粒后细胞膜和细胞质均有绿色荧光表达。激光共聚焦显微镜下可见CD59抗体刺激前CD59和LAT分子均匀分布于细胞膜,且转染干扰质粒的Jurkat-GFP细胞CD59表达量下降。CD59抗体刺激后CD59分子和LAT分子点状聚集共定位于细胞膜。MTT结果表明相对于正常Jurkat-GFP细胞,抗体活化后细胞增殖速率明显升高(P>0.05),而电转干扰质粒后细胞生长减慢。Western blot结果表明,CD59抗体刺激后细胞ZAP-70、LCK、PLC-r总蛋白表达无明显差异(P>0.05),而磷酸化蛋白表达显著增高(P<0.05)。结论:抗体活化后细胞膜上CD59和LAT分子聚集并共定位于细胞膜,且细胞信号转导通路下游分子磷酸化水平增高,进一步证实CD59分子经抗体活化后可促进LAT介导的T细胞信号转导。
