BACKGROUND Diabetic intracerebral hemorrhage(ICH)is a serious complication of diabetes.The role and mechanism of bone marrow mesenchymal stem cell(BMSC)-derived exosomes(BMSC-exo)in neuroinflammation post-ICH in patie...BACKGROUND Diabetic intracerebral hemorrhage(ICH)is a serious complication of diabetes.The role and mechanism of bone marrow mesenchymal stem cell(BMSC)-derived exosomes(BMSC-exo)in neuroinflammation post-ICH in patients with diabetes are unknown.In this study,we investigated the regulation of BMSC-exo on hyperglycemia-induced neuroinflammation.AIM To study the mechanism of BMSC-exo on nerve function damage after diabetes complicated with cerebral hemorrhage.METHODS BMSC-exo were isolated from mouse BMSC media.This was followed by transfection with microRNA-129-5p(miR-129-5p).BMSC-exo or miR-129-5poverexpressing BMSC-exo were intravitreally injected into a diabetes mouse model with ICH for in vivo analyses and were cocultured with high glucoseaffected BV2 cells for in vitro analyses.The dual luciferase test and RNA immunoprecipitation test verified the targeted binding relationship between miR-129-5p and high-mobility group box 1(HMGB1).Quantitative polymerase chain reaction,western blotting,and enzyme-linked immunosorbent assay were conducted to assess the levels of some inflammation factors,such as HMGB1,interleukin 6,interleukin 1β,toll-like receptor 4,and tumor necrosis factorα.Brain water content,neural function deficit score,and Evans blue were used to measure the neural function of mice.RESULTS Our findings indicated that BMSC-exo can promote neuroinflammation and functional recovery.MicroRNA chip analysis of BMSC-exo identified miR-129-5p as the specific microRNA with a protective role in neuroinflammation.Overexpression of miR-129-5p in BMSC-exo reduced the inflammatory response and neurological impairment in comorbid diabetes and ICH cases.Furthermore,we found that miR-129-5p had a targeted binding relationship with HMGB1 mRNA.CONCLUSION We demonstrated that BMSC-exo can reduce the inflammatory response after ICH with diabetes,thereby improving the neurological function of the brain.展开更多
Fifth Generation(5G)communications are regarded as the cornerstone to household consumer experience improvements and smart manufacturing revolution from the standpoint of industries’objectives.It is anticipated that ...Fifth Generation(5G)communications are regarded as the cornerstone to household consumer experience improvements and smart manufacturing revolution from the standpoint of industries’objectives.It is anticipated that Envisaged 5G(E5G)mobile technology would be operational in certain developed countries by 2023.The Internet of Things(IoTs)will transform how humans live when combined with smart and integrated sensing devices,such as in-home sensing devices.Recent research is being carried out all over the world to produce a new technique that can be crucial in the success of the anticipated 5G mobile technology.High output,reduced latency,highly reliable,greater scalability,high performance,capacity,bandwidth efficiency,virtual open-air transmission,and efficient energy mobile wireless communications are all being investigated currently.In this work,a comprehensive path for addressing the difficulties and developments associated with 5G mobile technology is provided.The debate and description of a complete analysis of current situations,certain characteristics and prospective scenarios,important technologies,problems and advances,and spectrum allocation of envisioned 5G mobile technologies are provided.Furthermore,this paper analyzes the most notable elements of 5G mobile technology,such as Cognitive Radio(CR),flexibility,accessibility,and cloud-based service offers,which will assure 5G mobile technology’s dominance as the main protocol for international communication.Eventually,this paper provides a method for integrating CR with current wireless communication systems,the necessity for further evolution of the E5G network,and the need for comprehensive consideration of architecture evolution and function enhancement to enhance the E5G mobile technologies.展开更多
Mobile Edge Computing(MEC) is an emerging technology in 5G era which enables the provision of the cloud and IT services within the close proximity of mobile subscribers.It allows the availability of the cloud servers ...Mobile Edge Computing(MEC) is an emerging technology in 5G era which enables the provision of the cloud and IT services within the close proximity of mobile subscribers.It allows the availability of the cloud servers inside or adjacent to the base station.The endto-end latency perceived by the mobile user is therefore reduced with the MEC platform.The context-aware services are able to be served by the application developers by leveraging the real time radio access network information from MEC.The MEC additionally enables the compute intensive applications execution in the resource constraint devices with the collaborative computing involving the cloud servers.This paper presents the architectural description of the MEC platform as well as the key functionalities enabling the above features.The relevant state-of-the-art research efforts are then surveyed.The paper finally discusses and identifies the open research challenges of MEC.展开更多
基金Supported by the National Natural Science Foundation of China,No.81900743Heilongjiang Province Outstanding Young Medical Talents Training Grant Project,China,No.HYD2020YQ0007.
文摘BACKGROUND Diabetic intracerebral hemorrhage(ICH)is a serious complication of diabetes.The role and mechanism of bone marrow mesenchymal stem cell(BMSC)-derived exosomes(BMSC-exo)in neuroinflammation post-ICH in patients with diabetes are unknown.In this study,we investigated the regulation of BMSC-exo on hyperglycemia-induced neuroinflammation.AIM To study the mechanism of BMSC-exo on nerve function damage after diabetes complicated with cerebral hemorrhage.METHODS BMSC-exo were isolated from mouse BMSC media.This was followed by transfection with microRNA-129-5p(miR-129-5p).BMSC-exo or miR-129-5poverexpressing BMSC-exo were intravitreally injected into a diabetes mouse model with ICH for in vivo analyses and were cocultured with high glucoseaffected BV2 cells for in vitro analyses.The dual luciferase test and RNA immunoprecipitation test verified the targeted binding relationship between miR-129-5p and high-mobility group box 1(HMGB1).Quantitative polymerase chain reaction,western blotting,and enzyme-linked immunosorbent assay were conducted to assess the levels of some inflammation factors,such as HMGB1,interleukin 6,interleukin 1β,toll-like receptor 4,and tumor necrosis factorα.Brain water content,neural function deficit score,and Evans blue were used to measure the neural function of mice.RESULTS Our findings indicated that BMSC-exo can promote neuroinflammation and functional recovery.MicroRNA chip analysis of BMSC-exo identified miR-129-5p as the specific microRNA with a protective role in neuroinflammation.Overexpression of miR-129-5p in BMSC-exo reduced the inflammatory response and neurological impairment in comorbid diabetes and ICH cases.Furthermore,we found that miR-129-5p had a targeted binding relationship with HMGB1 mRNA.CONCLUSION We demonstrated that BMSC-exo can reduce the inflammatory response after ICH with diabetes,thereby improving the neurological function of the brain.
文摘Fifth Generation(5G)communications are regarded as the cornerstone to household consumer experience improvements and smart manufacturing revolution from the standpoint of industries’objectives.It is anticipated that Envisaged 5G(E5G)mobile technology would be operational in certain developed countries by 2023.The Internet of Things(IoTs)will transform how humans live when combined with smart and integrated sensing devices,such as in-home sensing devices.Recent research is being carried out all over the world to produce a new technique that can be crucial in the success of the anticipated 5G mobile technology.High output,reduced latency,highly reliable,greater scalability,high performance,capacity,bandwidth efficiency,virtual open-air transmission,and efficient energy mobile wireless communications are all being investigated currently.In this work,a comprehensive path for addressing the difficulties and developments associated with 5G mobile technology is provided.The debate and description of a complete analysis of current situations,certain characteristics and prospective scenarios,important technologies,problems and advances,and spectrum allocation of envisioned 5G mobile technologies are provided.Furthermore,this paper analyzes the most notable elements of 5G mobile technology,such as Cognitive Radio(CR),flexibility,accessibility,and cloud-based service offers,which will assure 5G mobile technology’s dominance as the main protocol for international communication.Eventually,this paper provides a method for integrating CR with current wireless communication systems,the necessity for further evolution of the E5G network,and the need for comprehensive consideration of architecture evolution and function enhancement to enhance the E5G mobile technologies.
文摘Mobile Edge Computing(MEC) is an emerging technology in 5G era which enables the provision of the cloud and IT services within the close proximity of mobile subscribers.It allows the availability of the cloud servers inside or adjacent to the base station.The endto-end latency perceived by the mobile user is therefore reduced with the MEC platform.The context-aware services are able to be served by the application developers by leveraging the real time radio access network information from MEC.The MEC additionally enables the compute intensive applications execution in the resource constraint devices with the collaborative computing involving the cloud servers.This paper presents the architectural description of the MEC platform as well as the key functionalities enabling the above features.The relevant state-of-the-art research efforts are then surveyed.The paper finally discusses and identifies the open research challenges of MEC.
