目的构建香菇C91-3 cDNA文库。方法低速离心法收集香菇C91-3菌丝体发酵液中的菌丝,提取总RNA,巢式PCR扩增5SrRNA非转录间隔区2片段并测序鉴定。纯化mRNA,以mRNA为模板进行反转录反应;产物与pBlue-Script II SK(+)载体连接,并转化E.coil ...目的构建香菇C91-3 cDNA文库。方法低速离心法收集香菇C91-3菌丝体发酵液中的菌丝,提取总RNA,巢式PCR扩增5SrRNA非转录间隔区2片段并测序鉴定。纯化mRNA,以mRNA为模板进行反转录反应;产物与pBlue-Script II SK(+)载体连接,并转化E.coil DH10B,检测库容及插入片段。结果香菇C91-3cDNA文库初始库容为7.2×106cfu,插入片段长度0.4～3.4kb,平均1.5kb。结论成功构建香菇C91-3 cDNA文库,适合用于研究香菇蛋白的编码基因。展开更多
Aim To investigate the plant origin and the identity of the red substance on the surface of Hong Dangshen, a unique medicinal material for diarrhea in Hong Kong. Methods The HPLC fingerprints and 5S rRNA gene spacer s...Aim To investigate the plant origin and the identity of the red substance on the surface of Hong Dangshen, a unique medicinal material for diarrhea in Hong Kong. Methods The HPLC fingerprints and 5S rRNA gene spacer sequences of Hong Dangshen were obtained and compared with those of genuine species of Radix Codonopsis. The X-ray diffraction spectrum of the red substance was analyzed and compared with that of Halloysitum fingerprints to the Codonopsis species and the highest similarity to Rubrum. Results Hong Dangshen showed very similar HPLC Codonopsis pilosula var. modesta in terms of 5S rRNA gene spacer sequences. The X-ray diffraction spectrum of the red substance was consistent with that of Halloysitum Rubrum. Conclusion The source plant of Hong Dangshen was suggested to be Codonopsis pilosula var. modesta, one of the genuine original plants of Radix Codonopsis （Dangshen） in the China Pharmacopoeia （2005 edition）. The red substance on the surface of Hong Dangshen was indicated to be Halloysitum Rubrum, a traditional medicinal mineral for chronic diarrhea. Our data suggest that Hong Dangshen is derived from the roots of Codonopsis pilosula var. modesta which has been processed with Halloysitum Rubrum, and the name is suggested to be Radix Codonopsis Praeparata Halloysita Rubra.展开更多
基金Hong Kong Jockey Club Charities Trust (ProjectNo. HKJCICM-2-02R).
文摘Aim To investigate the plant origin and the identity of the red substance on the surface of Hong Dangshen, a unique medicinal material for diarrhea in Hong Kong. Methods The HPLC fingerprints and 5S rRNA gene spacer sequences of Hong Dangshen were obtained and compared with those of genuine species of Radix Codonopsis. The X-ray diffraction spectrum of the red substance was analyzed and compared with that of Halloysitum fingerprints to the Codonopsis species and the highest similarity to Rubrum. Results Hong Dangshen showed very similar HPLC Codonopsis pilosula var. modesta in terms of 5S rRNA gene spacer sequences. The X-ray diffraction spectrum of the red substance was consistent with that of Halloysitum Rubrum. Conclusion The source plant of Hong Dangshen was suggested to be Codonopsis pilosula var. modesta, one of the genuine original plants of Radix Codonopsis （Dangshen） in the China Pharmacopoeia （2005 edition）. The red substance on the surface of Hong Dangshen was indicated to be Halloysitum Rubrum, a traditional medicinal mineral for chronic diarrhea. Our data suggest that Hong Dangshen is derived from the roots of Codonopsis pilosula var. modesta which has been processed with Halloysitum Rubrum, and the name is suggested to be Radix Codonopsis Praeparata Halloysita Rubra.
