ENHANCEMENT OF DNA SYNTHESIS IN CULTURED ADULT RAT HEPATOCYTES BY 5-HT THROUGH STIMULATION OF 5-HT_2RECEPTOR

Hepatocytes were isolated from livers of adult male SpragueDawley rats and cultured in Williams'E Medium with 3 H thymidine. The effect of 5hydroxytryptamine (5HT) was investigated through adding various concentrations (10-810-3 mol/L) of 5HT to the hepatocyte cultures in the presence or absence of epidermal growth factor (EGF) and insulin. The involvement of 5HT2 receptor was examined by adding a 5HT2 receptor antagonist, ketanserin (10-6 mol/L), to some of the cultures containing 5HT. The increment of DNA synthesis was measured by 3 H thymidine incorporation. The results showed that 5HT2 (10-6 mol/L) significantly (P<005) increased the amount of DNA synthesis induced by EGF and insulin in the cultured adult rat hepaptocytes. The effect of 5HT in enhancing DNA synthesis began to appear at a concentration between 10-7 and 10-6 mol/L and reached maximum at concentrations of 10-4 mol/L. The enhancement of DNA synthesis by 5HT was significantly (P<005) antagonized by ketanserin, suggesting that this effect of 5HT was mediated by 5HT2 receptor subtype.

Correlation between psychological factors and the expression of galanin and 5‑hydroxytryptamine in different subtypes of gastroesophageal reflux disease

Objective:The aim of this study is to analyze the differences in galanin(GAL)and 5‑hydroxytryptamine(5‑HT)levels in patients with different subtypes of gastroesophageal reflux disease(GERD)and its correlation with anxiety and depression in patients.Methods:Using the reflux disease questionnaire,238 patients with GERD were selected as the study group.According to the endoscopic performance,they were divided into the nonerosive reflux disease(NERD)group(114 patients)and the RE group(124 patients).Sixty healthy people were selected as the control group.All research subjects were tested using the self‑rating anxiety scale(SAS)and the self‑rating depression scale(SDS)to assess the severity of anxiety and depression.The levels of 5‑HT and GAL were analyzed using enzyme‑linked immunosorbent assay.Results:Compared with the control group,5‑HT level in GERD patients without anxiety and depression was higher(t=1.97,P<0.05)and GAL level was lower(t=1.97,P<0.05).NERD patients demonstrated more server anxiety(F=55.92,P<0.05)and depression problems(F=36.51,P<0.05)compared to reflux esophagitis(RE)patients.The 5‑HT level was lower(F=54.53,P<0.05)and the GAL level was higher(F=8.00,P<0.05)in NERD patients compared with the control group.Furthermore,5‑HT level was negatively correlated with SAS(r=−0.789,P<0.05)and SDS(r=−0.787,P<0.05)scores;GAL level was positively correlated with SAS(r=0.688,P<0.05)and SDS(r=0.705,P<0.05)scores;5‑HT and GAL level were negatively correlated(r=−0.744,P<0.05).Conclusions:5‑HT level is higher and GAL level was lower in GERD patients without anxiety and depression than healthy people;the symptoms of anxiety and depression of NERD patients are more severe compared to those of RE patients;the severity of anxiety and depression was negatively correlated with the level of 5‑HT and positively with GAL level.

Synthesis of Oligomer with N-(O,O-diisopropyl)-phosphoryl Arginine and Its Effect on 5-Hydroxytryptamine Receptor

Oligomer (OMPA) with N(O,Odiisopropyl) phosphoryl arginine (DIPPArg) has been synthesized and its structure has been identified by IR, 1H NMR,31P NMR, and elemental analysis. The functional conversion ratio of this reaction was found to be about 30%. The effect of OMPA on the 5hydroxytryptamine (5HT) receptor was studied by a functional test of constriction in rat blood vessels. The results showed that OMPA inhibited 5HT receptorinduced blood vessels constrictions with pD2 values of 565±021 (OMPA) and 745± 030 (DIPPArg) and exhibited significant pharmacological action of antagonism effect on the 5HT receptor.