目的:建立一种远志中远志酮Ⅲ和3,6'-二芥子酰基蔗糖含量的HPLC-MS/MS分析方法。方法:采用ZORBAX Eclipse plus C18色谱柱,(4.6 mm×100 mm,3.5μm),流动相乙腈-0.1%甲酸水(28∶72),流速0.3 m L·min^-1,进样量10μL,...目的:建立一种远志中远志酮Ⅲ和3,6'-二芥子酰基蔗糖含量的HPLC-MS/MS分析方法。方法:采用ZORBAX Eclipse plus C18色谱柱,(4.6 mm×100 mm,3.5μm),流动相乙腈-0.1%甲酸水(28∶72),流速0.3 m L·min^-1,进样量10μL,柱温20℃。质谱条件:电喷雾负离子化模式,多反应监测模式(MRM),检测离子对分别为m/z 567.2~345.1(远志酮Ⅲ),m/z 753.3~205.1(3,6'-二芥子酰基蔗糖)。结果:两种成分的线性关系良好,分析过程只需6 min。远志酮Ⅲ的日内精密度和日间精密度的RSD分别为2.2%,2.5%,回收率为96.1%~101.7%(RSD 2.4%);3,6'-二芥子酰基蔗糖的日内精密度和日间精密度的RSD分别为2.3%,2.4%,回收率为95.7%~101.4%(RSD 2.0%)。结论:该方法快速、灵敏、选择性好,适合远志中远志酮Ⅲ和3,6'-二芥子酰基蔗糖的定量分析。展开更多
AIM:To simplify the procedure for synthesis of 2′ acetyl 12 hydroxy 2,3,10,11 dianhydro 6 O methyl erythromycin A(7). METHOD:Selective removal of the cladinose residue of clarithromycin was accomplished by the treatm...AIM:To simplify the procedure for synthesis of 2′ acetyl 12 hydroxy 2,3,10,11 dianhydro 6 O methyl erythromycin A(7). METHOD:Selective removal of the cladinose residue of clarithromycin was accomplished by the treatment with aqueous HCl at room temperature. Acetylation with acetic anhydride/triethylamine in methylene chloride provided the 2′ protected macrolide(9) with high yield. Then 2′ acetyl 12 hydroxy 2,3,10,11 dianhydro 6 O methyl erythromycin A(7) was synthesized from 9 which was treated with excess p toluenesulfonic acid and triethyl orthoformate at 50 ℃ in acetone.RESULT:The overall yield was 27%. The structure of the product was confirmed by IR,MS,NMR and elemental analysis. CONCLUSION: Our synthetic procedure of 7 was shorter and more convenient than that reported by Abbott Laboratories.展开更多
文摘AIM:To simplify the procedure for synthesis of 2′ acetyl 12 hydroxy 2,3,10,11 dianhydro 6 O methyl erythromycin A(7). METHOD:Selective removal of the cladinose residue of clarithromycin was accomplished by the treatment with aqueous HCl at room temperature. Acetylation with acetic anhydride/triethylamine in methylene chloride provided the 2′ protected macrolide(9) with high yield. Then 2′ acetyl 12 hydroxy 2,3,10,11 dianhydro 6 O methyl erythromycin A(7) was synthesized from 9 which was treated with excess p toluenesulfonic acid and triethyl orthoformate at 50 ℃ in acetone.RESULT:The overall yield was 27%. The structure of the product was confirmed by IR,MS,NMR and elemental analysis. CONCLUSION: Our synthetic procedure of 7 was shorter and more convenient than that reported by Abbott Laboratories.